LOCUS       NZ_CP066017            10308 bp    DNA     linear   CON 30-NOV-2023
DEFINITION  Acinetobacter baumannii strain FDAARGOS_1036 plasmid unnamed.
ACCESSION   NZ_CP066017
VERSION     NZ_CP066017.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357205
            Assembly: GCF_016127515.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   Kerrigan,L., Long,C., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1314.88x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:53:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,916
            CDSs (total)                      :: 3,820
            Genes (coding)                    :: 3,740
            CDSs (with protein)               :: 3,740
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 44 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 12 of 80
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1036"
                     /culture_collection="ATCC:BAA-1605D-5"
                     /db_xref="taxon:470"
                     /plasmid="unnamed"
                     /geo_loc_name="USA: VA"
                     /collected_by="ATCC"
     gene            <1..432
                     /locus_tag="I6H93_RS19460"
                     /old_locus_tag="I6H93_19460"
     CDS             <1..432
                     /locus_tag="I6H93_RS19460"
                     /old_locus_tag="I6H93_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_198493667.1"
                     /translation="SDQQKKIISLKQRINANVLVQDGDQPSEDPNELLKQVFSLIQEFQ
                     KLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRESDRYSTREIKW
                     HKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(755..928)
                     /locus_tag="I6H93_RS19465"
                     /old_locus_tag="I6H93_19465"
     CDS             complement(755..928)
                     /locus_tag="I6H93_RS19465"
                     /old_locus_tag="I6H93_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(928..1299)
                     /locus_tag="I6H93_RS19470"
                     /old_locus_tag="I6H93_19470"
     CDS             complement(928..1299)
                     /locus_tag="I6H93_RS19470"
                     /old_locus_tag="I6H93_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(1571..1825)
                     /locus_tag="I6H93_RS19745"
     CDS             complement(1571..1825)
                     /locus_tag="I6H93_RS19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            2509..3459
                     /gene="repM"
                     /locus_tag="I6H93_RS19475"
                     /old_locus_tag="I6H93_19475"
     CDS             2509..3459
                     /gene="repM"
                     /locus_tag="I6H93_RS19475"
                     /old_locus_tag="I6H93_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            3452..4027
                     /locus_tag="I6H93_RS19480"
                     /old_locus_tag="I6H93_19480"
     CDS             3452..4027
                     /locus_tag="I6H93_RS19480"
                     /old_locus_tag="I6H93_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            4047..4190
                     /locus_tag="I6H93_RS19485"
                     /old_locus_tag="I6H93_19485"
     CDS             4047..4190
                     /locus_tag="I6H93_RS19485"
                     /old_locus_tag="I6H93_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(4379..4897)
                     /locus_tag="I6H93_RS19490"
                     /old_locus_tag="I6H93_19490"
     CDS             complement(4379..4897)
                     /locus_tag="I6H93_RS19490"
                     /old_locus_tag="I6H93_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            5270..5557
                     /locus_tag="I6H93_RS19495"
                     /old_locus_tag="I6H93_19495"
     CDS             5270..5557
                     /locus_tag="I6H93_RS19495"
                     /old_locus_tag="I6H93_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5544..5858
                     /locus_tag="I6H93_RS19500"
                     /old_locus_tag="I6H93_19500"
     CDS             5544..5858
                     /locus_tag="I6H93_RS19500"
                     /old_locus_tag="I6H93_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            5986..8397
                     /gene="znuD2"
                     /locus_tag="I6H93_RS19505"
                     /old_locus_tag="I6H93_19505"
     CDS             5986..8397
                     /gene="znuD2"
                     /locus_tag="I6H93_RS19505"
                     /old_locus_tag="I6H93_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            8702..9163
                     /locus_tag="I6H93_RS19510"
                     /old_locus_tag="I6H93_19510"
     CDS             8702..9163
                     /locus_tag="I6H93_RS19510"
                     /old_locus_tag="I6H93_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(9486..9659)
                     /locus_tag="I6H93_RS19515"
                     /old_locus_tag="I6H93_19515"
     CDS             complement(9486..9659)
                     /locus_tag="I6H93_RS19515"
                     /old_locus_tag="I6H93_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(9659..10030)
                     /locus_tag="I6H93_RS19520"
                     /old_locus_tag="I6H93_19520"
     CDS             complement(9659..10030)
                     /locus_tag="I6H93_RS19520"
                     /old_locus_tag="I6H93_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     Typing          2510..3459
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtgatcaac aaaaaaaaat tatttcacta aaacaacgta tcaatgcaaa tgtattggta
       61 caagatggtg atcaaccatc tgaagatcca aatgaactac taaaacaagt attttcttta
      121 attcaagaat ttcaaaaatt aagttatgca attcatgaaa ctaatgcttt aacaaaatta
      181 aatgatggcc gttctctact tgctttattg actttgcgtg atgaatttgt agaacagcac
      241 aaaaccctaa ctgcagcaat aagtaataca tctcgtgaat ctgatcgtta tagtacgcgg
      301 gaaataaagt ggcataaagt tattccagtt tcatcattac aaaaacaagc tgatgatatt
      361 agtcttaaat tacgtgattt aaacgtgtta attcagtcga ctaattggaa aattgatttg
      421 attgaagcct aatagtcctt tagatataat caagttgaac atactgggcg agtacaagat
      481 cccttacttc cacaacatag gattgtaaat aaagtgtagt ggcttgcaaa gcaagtggat
      541 aagcttaaaa ttttaagtac aaatcaatcc atcacttggc ataactcaaa tctttgtgca
      601 aacaaagtta cacgttactt ataactacca tgtactgaca gtatgttcac ctcaattact
      661 aaaaataaag aaatgaacaa gcatcagctc aactacattt ctatttttaa ttcagttcgg
      721 catttaacat aaaatctctt atacgaagcc caggttaatt ctgggctttt tgttcaatct
      781 catcattggc cgagaacata cttggattcg taagcctgat attttcctga caacttccac
      841 acagcatcaa tcctgcaaaa agttgtacat tttgtctatt ttcacaaagc gtacatggct
      901 ttgaaagctg aataatatta tctgacacta ctttaccctc ttatagttat catctgcata
      961 tgaataacct ttagcttgta tcattctaac ctcatcttta gaagcgccat ttttcattaa
     1021 gagccacaac atcatgaaat atagtccaga aaagtcttca tagtattttt cagaaaaatt
     1081 gttatcttcc tcaaaatcat ctattcgctt ttctaatcga ttcaccagat cctcaatacg
     1141 atcggcttgc tgtttaacag caatagattg aaaatcattg gtcaaaattt tactgaataa
     1201 tttactggtt ctgattcctt ctgaattagc caattgatcg tagaattctg ctacttgtgg
     1261 atctaacctg actgcataag ttttgacttt ttcgcccata aattacacct cttattaaaa
     1321 ttagattttg tacaaaatta aatatatata attcaactaa ttaaataaaa aataataatt
     1381 agattacgta caaacttatt tgtacagaat taatttattc taaaacaaaa acttatttaa
     1441 atataatgct ttttttatgt acaatcatga gtacatattt tttcttaaat ttatcaatat
     1501 cttatctaaa aaaagtgttg ttttgatgta caaccatgag cacagcataa aaaatagata
     1561 attcaatcac ttattctaaa aaaagaccat ttttctgtac acgtttctga gaccctaaaa
     1621 aatcgctgaa atccttatcc tgtaaggctt tgaggggtgt catcgttaga tgcgtggtgt
     1681 gtgacaaaaa aatcccggca tgtgccggat cttggattgg aaaattggct aaagtgacgt
     1741 agggccggtg cttggttttg catggaaaaa agtatttatt tccttgttta gaaaaacgta
     1801 aaaaatcaat tttttgtcca ttcatccagg ttaaaaattt caacccaaaa ctttaattat
     1861 gaaaagcttc atagaacgcg ttcaaatgcg atttaagagc ctttacctag aaaacataga
     1921 tctcatagtg aaaaacacaa aacagctcaa aaaacgcaaa tgagagcgaa ataaagaggt
     1981 attttgactt tagatagatg catagagcga gtgtctacga gcgaactatc aaaatttgcg
     2041 cctagaccct ctgaaaaaca tttttttgtc ctctttagcc taagaaagcg taagtttcgt
     2101 gcagaaattt gctcctggat cgagcgtagc gagtaaaaaa gttttatgag cgaagcgaat
     2161 tccaagtttc ttttgctttt ttttaaagtc acgccagtat taaccaaaaa attgccccaa
     2221 cgaactgagc gaaagcgaag ttcaatagag tttgagcgaa gcgaaaacca agggcaattt
     2281 ttcattccct gggcttttaa ttattttaaa gtttttaaat acttttaaat ggcttgaaag
     2341 ccttatgaat aaagggtttt agctcttata tgtccacgtt taccttgcaa tatgtccacg
     2401 tttaccttgc aatatgtcca cgtttacctt gcaatatgtc cacgtttacc ttgcattagt
     2461 acacaaataa tattaacgtg tacttataca caataaaaaa tagtggctat gagagattta
     2521 gttgtaaagg acaatgcctt aatcaacgca agctataact tagacttagt agaacaacgt
     2581 ttaattttat tggctattgt tgaagcaagg gaaagtggga aagggattaa tgcaaatgat
     2641 ccattagaag ttcatgcaga tagttatatc aatcaatttg gtgtacaccg taatacagct
     2701 tatcaagcct taaaagatgc ttgtaaggat ttattcgcgc gtcaatttag ctatcaagag
     2761 aaaaaagcta atgggaatat ccgaaatgtt atgagtcgtt gggtatctca aattgcttat
     2821 aacgacaatg aagcaactgt agacttaata tttgcacctg ctgttgttcc cttcataacc
     2881 cgactggaag aacaatttac taaatatgaa ttacagcaag ttagtagtct tagtagcgct
     2941 tatgccattc gcttatatga gcttctaatt cagtggcgaa gcactggtaa aaccccaacc
     3001 atagaactac aagaatttag aaagaagtta ggcgttcttg ataatgaata tttacggatg
     3061 gctcatttaa aagagcgtgt tttagagctt tcaattaaac aaataaatga gcatacggat
     3121 ataactgtaa aatatgaaca gcataaaaga ggacgttcta tttcaggatt ttcttttacc
     3181 tttaaacaga agaagaagga tagcccatca atagaaagag atccgaacac tttggagctt
     3241 ttttcaaaga tgaccgatgc tcaacggcat atgtttgcaa ataaactttc agaactccct
     3301 gaaatgggtc gctattcaca aggaacagaa agctaccaac agtttgctgt acgtattgct
     3361 gagatgctac aagatcccgc tcaattcaaa gagctatacc catacctaaa aaaagtggga
     3421 tacatgccat caaataaaaa ggacaccgta aatggctaag ttatcactaa gtgaagtatc
     3481 taaaaaattt catgtggata gatcaaccat ttacagagct gtacgtaatg gacgtttatc
     3541 acgctccagt gatggacaat tcgatctagc agaggtcata cgatgctttg gagaacctga
     3601 gcaaacatct caaaaaattg aatcatctaa gcaagaaggt gatgaatcta caaaaaaact
     3661 tattgcccat ttagaaaatg aagtcaaaaa ataccaagaa cgtgaagaac ggttaatgca
     3721 acaaattgac cgtatgcaaa cactcattga gctgaaaagt gttgcacctg ccacagcagc
     3781 accacaccaa gatgctacgg catgcgacac caagatgcca cagcatgcga caacacaaca
     3841 agacactgat aacaaaaaga ataatgaatt aaatattgca gaaaatgtag cagtgccaca
     3901 gcaagaaact acggcatacc acacccaaac gctacagcat gccacgttgc aaagtgtggc
     3961 agtgccacaa cacaaaaaac gtggtttatt tggccgtgtg ctgaatgccg tctttgataa
     4021 tgactgagca ataagggtga aagatcatgc cgaaactgaa agacattgcc ctgggaatta
     4081 ttgtagctcc gctgctgatc ccgatcatgc tgattgcatc gtaccaggat aaaaaggcgc
     4141 tgaagaaaga gctggatgaa tgccaaaaag aaaaagacca ggcatcctga ttattcaatt
     4201 gagctaatca aaagtgtgga acattgatga aataatagcg tggaacgtga aaagtcataa
     4261 aaaaagcccg aacagggatg ctcgggctat aaactagaaa ctacagcgtt gaattataag
     4321 gataattata cagcatttcg tataaggtgt attatgttaa ttttaggaaa tattaaaact
     4381 aattttcaga cttatcccac caactttgag ctaaatcagt atttttagga atacctatac
     4441 catcacgata cataacggaa agacttcgca ttgctttctt attacctaac tttgcagcct
     4501 tttcacgaag ttcaaaagct ttattaatat taacttctat tccgctatct gcacgagtat
     4561 atagatcagc taactcaacc attgattcag aatggccatt ttctgaggct ttctgaaacc
     4621 acttaaaagc tttttggaaa tgttctaaag aatcgttttt gtcatcatct tccattaatc
     4681 tagcataagt aacatatcca tcagctaacc aaaacattgc tgctacattt ccattttctg
     4741 ctgatttaag agcccaaact tcagcttgat tttcatctga atcaacacca tgaccatata
     4801 gataaatctc ggctaattct aactgagcag aagtatctcc attttctgct ttcgctaata
     4861 aatcattaga aaaaacaggt tctgcaaatg tttccattga aaggcaaact cctaaagcca
     4921 taaaaatatt tgttaattta ttcaattttt aacctttaaa ctaaactgtt aggcttgtat
     4981 atttttatat catacctaaa agaaaactct aaaattagag tatgtcttat ctctatttaa
     5041 cataatggcg attatacgaa gtacacttgt atattaatat aaatatcaac tacttaataa
     5101 aaatagaaaa atcccaaaat gcccaaaaag ccgactttga aacaagtcgg cttttttatg
     5161 agcagttttg atacttcttg ccaataattt tgactaaaaa ttacacgaat ctggttgagt
     5221 gatacaattt tgtgatacat tattgtatat acaaatttga atgtttttta tggaacagta
     5281 ttttgaatgg gatgaggcta agaatcgaaa gaatcagaaa aagcatgata tctctttcga
     5341 aactgcaagc cttgtttttg aagatccatt acggatctca atccaagaca gacataccaa
     5401 tggtgaagaa cgttggcaaa ccattggacg agttaaaggc gtactaatgt tattggtggc
     5461 tcacaccatt tttgatgaag atgactgtga aatcatccga atcattagtg caaggcaagt
     5521 cactaaagcg gagcgaaaca aatatgagca tggttagata ctcacacaaa gagctgaatg
     5581 agaaatttgg tgagaagcaa gatgctgaaa ttcaacgctt gcttgctaaa ggcacggttc
     5641 ctgatgatca gctagatcta tcagatattc ctgaaattac agactggagc aatgctgtac
     5701 gccaaaatca attttaccgc ccagtaaagc aacaaacttc aattcggtta gatgctgatg
     5761 tacttgcttg gttcaaagca caaggcaaag gttatcaaac acgtatgaat gaaattttac
     5821 gagatgctat gctaaaagaa ttaaaaaatc atcaataaga atgggagctt aggctcccat
     5881 ttttgacttc atttttaggg gggattacca ttcaaaaatt taaatagtag attttataaa
     5941 tttttaagtg ttatattata atataacatt aaatttggtt gttttatgct aaataaaagt
     6001 aaactatttt tagctttaat tactttaggg gcctcgaaaa ttttattggc agctgaaggg
     6061 cctgttacta ccttaaatac aatagtatta actgcacaat ctgatgagtt agggtctgag
     6121 ctgttaggca aaagtctaaa tgttagtaat cagtttattg atactagtaa acttaaacag
     6181 cgttcgacaa ctctagggga tgccttgggt acggaattag gaattcattc taatcaatat
     6241 ggaggtggag catctactcc tattattcgt gggcaggaag gaaaacgtat taaagtttta
     6301 cagaataatg ctgacgtgtt ggatatgtct aatatgtccc cagatcacgc tgtcacagtt
     6361 gaaccaagtt tagctaagtc tattgaaatt atacgtggtg caagtaccct tttgtatagt
     6421 tcaaatagtg cggcaggggt agtcaatgtc attgactata aaattccaac ccaaatgcct
     6481 caagacggat tagagggtaa tactacatta cgttttaata ctggcagtaa tgagaagttg
     6541 accacagcag gtgtaacagt tggcttaagc ccacatgtag ctttacgggc agaagggtta
     6601 tatcgtaatg ctggtaacta taaaactcct cattaccagt ctagctctta taattcttta
     6661 gaagatcttg aaaaccaaaa cagtatttat aaaaatttaa aatatttacc agaaagctgg
     6721 gcagagtcga gagtaggtac gcttggatta tcctggatag acgataatac ttatttagga
     6781 gtttcttata cacatcgtca tgatgaatat ggtttaccag cccatagcca tttatatgaa
     6841 ggttgtggtg cctctgcaat cggtatcgat tcacgaatta gtggattaaa aaattatctt
     6901 ttatattatc ctcaattgat ggatgagcaa gatattaatt acataaatcc acgtcctgat
     6961 tgtcatcagc ataatcatat tcatgaaact aatttctctc ataatgctcc ttatattgat
     7021 ttaaatactc gacgttatga tgtgcgtgga gaattcactc aaccatttac cggtattgac
     7081 aaaattcgta cctcattgag ttatatagat tattttcaca atgaactcga gggagataag
     7141 attactaact tttttaaaaa tacaggaaaa gtaggccgca tagagttatc acatcagcca
     7201 ttaggggaat taaccggtat cttggggcta caatatttgg aacaggataa tagtgcttta
     7261 tcaccagtcc attctcaaga aggccatacc acctatctag ataatcagca gctattaaat
     7321 cgaaatgtca ctaaaaactt tagtgtattt ggcctagaaa aatataactg gaacgacttt
     7381 actttcgaac taggtgctcg tatagaaaag cagaaagtta gtatggatta tgatatagaa
     7441 aaaattaaag attcaatgaa accttggcct aataaatata acagtcctta tgtagaaaaa
     7501 aataataaaa tcagggctca aaatttaaaa tctattttag aagctgttca acctaacaaa
     7561 gaaacagctt tttcttatgc aggtacagtc cactggagat ttgctccaaa ttatatctta
     7621 tcattaacag gtacacatca agaaagatta cctaatgcac aagaaatgta tactcatggt
     7681 atgcatctcg ctactaattc ttttgaaata ggtaaccgct ttctaagaaa agagaaatcc
     7741 aataatttag aaatcagtct ggcctataag gatgatttac ttgattatca aatcagtact
     7801 tactattatg attttgataa ctatatatat ttacagacac tcaatgaagt tttaggaact
     7861 acaaaagtta gagatcagca tacactacgt attaatcatt atagccagtc tgctgctaat
     7921 ttttatggat tagagggcaa catagggtat caatttaatt ctgtttatca cggctctctt
     7981 tttggagatt atgtgaaagg ccgtttaaca aatttacctg atgctgtaat tgcatacgat
     8041 atctggaata gagaaccgac tttagctccc caaaaggatc gttacactcc tagattacct
     8101 cctgctcgtt tgggtacacg actaaaagct gattttgatg aatcgcttaa aggtgaaata
     8161 gaatattatc gagtctttaa acaagataat atttctaaat ttgagcaggt tacatcaggt
     8221 tataatatgc ttaatatgac tttagcatat aaaaataagc tatctcacac agaatatgat
     8281 ttattcttta aagcgaataa tttacttgat cagaaagtct atgcccatga aacatttttg
     8341 ccatatattc cccaaattgg aagaaatttt agtttaggct taaatcttaa tttctagata
     8401 agatactgta atcttctaag aaaatttctt agaagatttt tcttataaaa tgattagatt
     8461 aataattaag gtaagcgtcc gctgaatccc atacctattt tttaattcga tttaggtttc
     8521 cagcagtgtg gcagtaactc ttcaatctgg gtcactttat gtgtcggcag ccttttcagc
     8581 actgaatagc caccgatttt gtagacacct tttagctaga taaaaagcaa tttttttgat
     8641 ctttaaagaa tgaggatata agctgtttaa aacttaattc taattggtta ataggtgaaa
     8701 aatgaaatta gcagaagctc tcttacttcg aagtgatcaa caaaaaaaaa ttatttcact
     8761 aaaacaacgt atcaatgcaa atgtattggt acaagatggt gatcaaccat ctgaagatcc
     8821 aaatgaacta ctaaaacaag tattttcttt aattcaagaa tttcaaaaat taagttatgc
     8881 aattcatgaa actaatgctt taacaaaatt aaatgatggc cgttctctac ttgctttatt
     8941 gactttgcgt gatgaatttg tagaacagca caaaacccta actgcagcaa taagtaatac
     9001 atctcgtgaa tctgatcgtt atagtacgcg ggaaataaag tggcataaag ttattccagt
     9061 ttcatcatta caaaaacaag ctgatgatat tagtcttaaa ttacgtgatt taaacgtgtt
     9121 aattcagtcg actaattgga aaattgattt gattgaagcc taatagtcct ttagatataa
     9181 tcaagttgaa catactgggc gagtacaaga tcccttactt ccacaacata ggattgtaaa
     9241 taaagtgtag tggcttgcaa agcaagtgga taagcttaaa attttaagta caaatcaatc
     9301 catcacttgg cataactcaa atctttgtgc aaacaaagtt acacgttact tataactacc
     9361 atgtactgac agtatgttca cctcaattac taaaaataaa gaaatgaaca agcatcagct
     9421 caactacatt tctattttta attcagttcg gcatttaaca taaaatctct tatacgaagc
     9481 ccaggttaat tctgggcttt ttgttcaatc tcatcattgg ccgagaacat acttggattc
     9541 gtaagcctga tattttcctg acaacttcca cacagcatca atcctgcaaa aagttgtaca
     9601 ttttgtctat tttcacaaag cgtacatggc tttgaaagct gaataatatt atctgacact
     9661 actttaccct cttatagtta tcatctgcat atgaataacc tttagcttgt atcattctaa
     9721 cctcatcttt agaagcgcca tttttcatta agagccacaa catcatgaaa tatagtccag
     9781 aaaagtcttc atagtatttt tcagaaaaat tgttatcttc ctcaaaatca tctattcgct
     9841 tttctaatcg attcaccaga tcctcaatac gatcggcttg ctgtttaaca gcaatagatt
     9901 gaaaatcatt ggtcaaaatt ttactgaata atttactggt tctgattcct tctgaattag
     9961 ccaattgatc gtagaattct gctacttgtg gatctaacct gactgcataa gttttgactt
    10021 tttcgcccat aaattacacc tcttattaaa attagatttt gtacaaaatt aaatatatat
    10081 aattcaacta attaaataaa aaataataat tagattacgt acaaacttat ttgtacagaa
    10141 ttaatttatt ctaaaacaaa aacttattta aatataatgc tttttttatg tacaatcatg
    10201 agtacatatt ttttcttaaa tttatcaata tcttatctaa aaaaagtgtt gttttgatgt
    10261 acaaccatga gcacagcata aaaaatagat aattcaatca cttattct
//
