LOCUS       NZ_CP066008             8688 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Salmonella enterica strain FDAARGOS_1067 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP066008
VERSION     NZ_CP066008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357236
            Assembly: GCF_016127435.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 7.1.0, HGAP v. 4
            Genome Coverage                   :: 730.77x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:46:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,975
            CDSs (total)                      :: 4,852
            Genes (coding)                    :: 4,724
            CDSs (with protein)               :: 4,724
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 53 of 128
            Pseudo Genes (incomplete)         :: 81 of 128
            Pseudo Genes (internal stop)      :: 28 of 128
            Pseudo Genes (multiple problems)  :: 31 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1067"
                     /culture_collection="DSM:24522"
                     /db_xref="taxon:28901"
                     /plasmid="unnamed1"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(8596..8688,1..744))
                     /locus_tag="I6I24_RS00005"
                     /old_locus_tag="I6I24_00005"
     CDS             complement(join(8596..8688,1..744))
                     /locus_tag="I6I24_RS00005"
                     /old_locus_tag="I6I24_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(745..1163)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(748..1547)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1608..2423)
                     /gene="sul2"
                     /locus_tag="I6I24_RS00015"
                     /old_locus_tag="I6I24_00015"
     ARG             complement(1612..2423)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2731..3582)
                     /gene="repC"
                     /locus_tag="I6I24_RS00020"
                     /old_locus_tag="I6I24_00020"
     CDS             complement(2731..3582)
                     /gene="repC"
                     /locus_tag="I6I24_RS00020"
                     /old_locus_tag="I6I24_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(3569..4408)
                     /locus_tag="I6I24_RS00025"
                     /old_locus_tag="I6I24_00025"
     CDS             complement(3569..4408)
                     /locus_tag="I6I24_RS00025"
                     /old_locus_tag="I6I24_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(4438..4644)
                     /gene="urfF"
                     /locus_tag="I6I24_RS00030"
                     /old_locus_tag="I6I24_00030"
     CDS             complement(4438..4644)
                     /gene="urfF"
                     /locus_tag="I6I24_RS00030"
                     /old_locus_tag="I6I24_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(4646..4858)
                     /locus_tag="I6I24_RS00035"
                     /old_locus_tag="I6I24_00035"
     CDS             complement(4646..4858)
                     /locus_tag="I6I24_RS00035"
                     /old_locus_tag="I6I24_00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(4919..5890)
                     /locus_tag="I6I24_RS24705"
     CDS             complement(4919..5890)
                     /locus_tag="I6I24_RS24705"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_010890158.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(5887..6300)
                     /locus_tag="I6I24_RS24710"
     CDS             complement(5887..6300)
                     /locus_tag="I6I24_RS24710"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<6455..7048)
                     /locus_tag="I6I24_RS24715"
                     /pseudo=""
     CDS             complement(<6455..7048)
                     /locus_tag="I6I24_RS24715"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            7247..7531
                     /locus_tag="I6I24_RS00045"
                     /old_locus_tag="I6I24_00045"
     CDS             7247..7531
                     /locus_tag="I6I24_RS00045"
                     /old_locus_tag="I6I24_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            8085..>8489
                     /locus_tag="I6I24_RS00050"
                     /old_locus_tag="I6I24_00050"
                     /pseudo=""
     CDS             8085..>8489
                     /locus_tag="I6I24_RS00050"
                     /old_locus_tag="I6I24_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     ARG             complement(2..744)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="89.21"
     Typing          complement(7138..7293)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(3570..4391)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(4923..7048)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga
       61 gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc
      121 cggatcgtag aacatattgg cggcgccaaa gcccacttca ccgaccagac cgacgggatc
      181 tatcaccagc cagccgcgac tggagaacat gatgttttca tgatgcagat cgccatgtag
      241 cccacgcagt tccgaggcat tgctcatcat ttgatcggct ataatcgccg cgtggacgta
      301 gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc tgaaacaaag ctgcaaagcg
      361 atcccggatc gggagaaggg cagaaggcag gggttcctca gatgcggcat acagcttcgc
      421 cattagttcc gctgcaattt cggtcgcctg gtagtcgccg tgctcggcaa cgatgtgaga
      481 gagcattcgc tccccggcat attcgagcaa catcagattg ttctcacgac cgagcaaccg
      541 gactgctccc ctcccattgc gccataccag atagtcggcc ccgcgcagtt catcagcaat
      601 gtcttctata ggtttcaatc ccttgacgat tgcaggagtc ccgtctggca atgaaacttt
      661 ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt tgcgaaacgt gccaatgagc
      721 aggaaaaaca ggcggcatga acatcaaccc caagtcagag ggtccaatcg cagatagaag
      781 gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat tgaataggac agcgaaggcg
      841 cgctctgctt catctggcgc tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa
      901 tcggcatagc gatctgctgt tccgagccgc ccaaggtcga tcagacccgt gcattgaaga
      961 gttttagggt ccaccatgaa gttcggcatg cagggatcac catggcaaac aaccatatcg
     1021 gtgcgctctt ggtcgagccg caccggtagc tctcgttcga cacgagccaa aagatcgagc
     1081 tgcggcgtac tcttgtcctc gtccggtaag aagtcgggat tgacggcatt gcgggacacc
     1141 acatcaacgg cgcgtccgaa cattcgcgac agcctgcgct caaacggaca ttgatcaacc
     1201 gataggctgt gaacagcgcc aagttgctgc cccattgacg gccacgcttt gagcaaatcc
     1261 gctccagaca gatcagccgc cggtactccc ggaattgccg ttatcaccaa gcatgcaccc
     1321 tcctgttcct cctgccagtt gatgacctcg gggcaagcca cacctcgacc tttgagccaa
     1381 atgaggcggt cacgctctcc agcgagctca ccgcggcggg aagcaggtgc gattttcgcg
     1441 aaggcatgcc cgtcaccacg tcgaaaaaca aaatcaccag attctccgcc tctgacaggc
     1501 aaccagtcag aatgcgattc accaaaaaaa atattagttc gattcaatgg aggttccttc
     1561 agttttctga tgaagcgcga atatagagaa atatcccgaa tgtgcagtta acgaattctt
     1621 gcggtttctt tcagcgccgc caataccgcc agcccgtcgc gcaaggggcg cggctcgtgt
     1681 gtgcggatga agtcagctcc acctgcggcg gcggcaagct ctgcagcgag tgtcgcggcc
     1741 ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa aggatttgcg cgaaacagac
     1801 agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga accgcgccag caccgagagc
     1861 gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg gatcaaggac aaggcggttg
     1921 cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt caaagaacgc cgcaatgtga
     1981 tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct gcccgtcttg caccgaatgc
     2041 ataacgacga gtttggcaga tgatttcgcc aattgcggat agaacgcagc gtctggaaaa
     2101 ccgcgaatat cattgagata ggccacacca cgcgacaagg cataggcttg cgtcgcgggt
     2161 tgataactgt cgagcgagac gggaatgcca tctgccttga gcgcgtccag caccggcgcg
     2221 atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt cgggattgct ggatgccgga
     2281 ccgaggtcga tcacatctgc cccctcggcc atcagcttac gcgcctgcgc aatggctgcg
     2341 tctggcgcca gataccggcc tccatcggag aaactgtccg aggttatgtt gacgatgccg
     2401 aaaatgatga gcgatttatt catgggggct tctataataa taataatcga gcatgagtat
     2461 catacggatg ctcgggtcga aagggaatcc ccaggcgagt aacctgtttg cggtgatcca
     2521 ttagctgcag gagcagaaga gcatacatct ggaagcaaag ccaggaaagc ggcctatgga
     2581 gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt ttctgagcat
     2641 ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat aaaagataaa
     2701 aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc
     2761 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
     2821 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
     2881 atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca
     2941 cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct
     3001 gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac
     3061 gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg
     3121 cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat
     3181 gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata
     3241 gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag
     3301 ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc
     3361 agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc
     3421 gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt
     3481 gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg
     3541 ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct
     3601 tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc
     3661 gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc
     3721 gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg
     3781 actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc
     3841 ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg
     3901 tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt
     3961 cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg
     4021 cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg
     4081 gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa
     4141 gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg
     4201 ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca
     4261 gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac
     4321 cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca
     4381 gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta
     4441 ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg
     4501 cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt
     4561 catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt
     4621 ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct
     4681 tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga
     4741 ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat
     4801 agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa
     4861 aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact
     4921 acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg
     4981 ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct
     5041 cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca
     5101 tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa
     5161 agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt
     5221 actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt tcgaggctgg
     5281 ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct
     5341 gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg
     5401 gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc
     5461 catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg
     5521 caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt
     5581 tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca
     5641 ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc
     5701 tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca
     5761 cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga
     5821 tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt
     5881 cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt
     5941 caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag
     6001 catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc
     6061 ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc
     6121 ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt
     6181 ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc
     6241 gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat
     6301 ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt
     6361 tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg
     6421 gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa
     6481 tgcgggcgtc gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc
     6541 gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct
     6601 ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat
     6661 tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg
     6721 ccagcgtata cggcaggcgc tcggcaccag tcaggtgctg ggcgaactcg gacgccagcg
     6781 ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc
     6841 gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga
     6901 actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc
     6961 cttcgcgctg gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg
     7021 ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc
     7081 catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga
     7141 agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc
     7201 ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag
     7261 caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga
     7321 aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa
     7381 ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg
     7441 gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct
     7501 gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
     7561 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
     7621 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
     7681 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
     7741 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
     7801 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
     7861 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
     7921 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac
     7981 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     8041 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     8101 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     8161 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     8221 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc
     8281 ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg
     8341 ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa
     8401 gtgccgcctt gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga
     8461 gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta
     8521 cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga
     8581 gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc
     8641 tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaag
//
