LOCUS       NZ_CP065951            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP2509 plasmid pKP2509-5, complete
            sequence.
ACCESSION   NZ_CP065951
VERSION     NZ_CP065951.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17053394
            Assembly: GCF_018279325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Lin,Y.
  TITLE     Genome sequencing of KP2509
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Lin,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2020) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 10:44:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,064
            CDSs (total)                      :: 5,940
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 53 of 155
            Pseudo Genes (incomplete)         :: 107 of 155
            Pseudo Genes (internal stop)      :: 18 of 155
            Pseudo Genes (multiple problems)  :: 20 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065951.1.
            Bacteria and source DNA available from Taipei Veterans General
            Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP2509"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP2509-5"
                     /geo_loc_name="Taiwan"
                     /collected_by="Taipei Veterans General Hospital"
     gene            join(9397..10060,1..1022)
                     /locus_tag="JDE60_RS27405"
                     /old_locus_tag="JDE60_27385"
     CDS             join(9397..10060,1..1022)
                     /locus_tag="JDE60_RS27405"
                     /old_locus_tag="JDE60_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1032..1289
                     /locus_tag="JDE60_RS27410"
                     /old_locus_tag="JDE60_27390"
     CDS             1032..1289
                     /locus_tag="JDE60_RS27410"
                     /old_locus_tag="JDE60_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1374..1523
                     /locus_tag="JDE60_RS27415"
                     /old_locus_tag="JDE60_27395"
     CDS             1374..1523
                     /locus_tag="JDE60_RS27415"
                     /old_locus_tag="JDE60_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3007..4971
                     /locus_tag="JDE60_RS27420"
                     /old_locus_tag="JDE60_27400"
     CDS             3007..4971
                     /locus_tag="JDE60_RS27420"
                     /old_locus_tag="JDE60_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4971..5702
                     /gene="mobC"
                     /locus_tag="JDE60_RS27425"
                     /old_locus_tag="JDE60_27405"
     CDS             4971..5702
                     /gene="mobC"
                     /locus_tag="JDE60_RS27425"
                     /old_locus_tag="JDE60_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5709..6239
                     /locus_tag="JDE60_RS27430"
                     /old_locus_tag="JDE60_27410"
     CDS             5709..6239
                     /locus_tag="JDE60_RS27430"
                     /old_locus_tag="JDE60_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6266..6445)
                     /locus_tag="JDE60_RS27435"
                     /old_locus_tag="JDE60_27415"
     CDS             complement(6266..6445)
                     /locus_tag="JDE60_RS27435"
                     /old_locus_tag="JDE60_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6471..6899)
                     /locus_tag="JDE60_RS27440"
                     /old_locus_tag="JDE60_27420"
     CDS             complement(6471..6899)
                     /locus_tag="JDE60_RS27440"
                     /old_locus_tag="JDE60_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7063..7377)
                     /locus_tag="JDE60_RS27445"
                     /old_locus_tag="JDE60_27425"
     CDS             complement(7063..7377)
                     /locus_tag="JDE60_RS27445"
                     /old_locus_tag="JDE60_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7365..7643)
                     /locus_tag="JDE60_RS27450"
                     /old_locus_tag="JDE60_27430"
     CDS             complement(7365..7643)
                     /locus_tag="JDE60_RS27450"
                     /old_locus_tag="JDE60_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7818..8183)
                     /locus_tag="JDE60_RS30385"
     CDS             complement(7818..8183)
                     /locus_tag="JDE60_RS30385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8180..8551)
                     /gene="tolA"
                     /locus_tag="JDE60_RS27460"
                     /old_locus_tag="JDE60_27440"
     CDS             complement(8180..8551)
                     /gene="tolA"
                     /locus_tag="JDE60_RS27460"
                     /old_locus_tag="JDE60_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8825..9070)
                     /locus_tag="JDE60_RS27465"
                     /old_locus_tag="JDE60_27445"
     CDS             complement(8825..9070)
                     /locus_tag="JDE60_RS27465"
                     /old_locus_tag="JDE60_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1627..2205)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4972..5699
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga
       61 gcgtaccgaa aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag
      121 gtgattcacg tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt
      181 tcccgaaaga gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg
      241 cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc
      301 atccggaaga ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag
      361 ataaaaatat tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg
      421 caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg
      481 attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct
      541 atcttgataa ggatattcag aatcagaatg agaaattaaa agcggctcag gcatctttaa
      601 acgcaatgaa tgaatcctta tccagagata aggctgcact taccggggcg atggagagcc
      661 ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca
      721 agcctcgcaa ggggactaaa gactacggcc atgattatca tccagtacct aaaacagaag
      781 acataaaggg acttggagag ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg
      841 gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa aatttatgaa tgggattccc
      901 agcacggtga gcttgaaggg tatcgtgcca gtgatggcga acacctaggc gcatttgatc
      961 ccaaaacggg taagcaggtt aaagggccag atccaaaacg gaacattaaa aaatatcttt
     1021 gagaggtaaa tatggggctt aaattacgtt tgacatggtt tgataagaaa actgaagagt
     1081 ttaaagggga agagtattct aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg
     1141 gaatgccttt aaaggataat attaataatg gtgagtttga tttgaagaaa gaatgggttc
     1201 ctttactaca gccatttttt aaaaatcaaa taaaacctga tgtggataat tgttatatag
     1261 cttttgatta tcgggacggt aactggtaac aaaaaattat caaggatgat ttgaaatact
     1321 gacttccggt gttcagggat gaatgccgga gtctttaaat acagggaaag tatatgaaaa
     1381 aggtaaagac tatttttctt tttatattga ttgttgctgg ctttcttctg gccgcatgtc
     1441 aggcaaatta tatccgggat gttcagggcg gaacggtggc accatcgtcc tcctctgaac
     1501 tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
     1561 gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg
     1621 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
     1681 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
     1741 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg
     1801 tgctttcccg ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga
     1861 acggggagtt cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac
     1921 cagtgtgtga gctatgagaa agcgccacac ttcccgcaag ggagaacggc ggacaggtat
     1981 ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg agcgacctac cggaaacggt
     2041 ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac
     2101 ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actctggcag gagggcggag
     2161 cctatggaaa aacgccaccg gcgcggccct gccgttttgc ctcacatgtt agtcccctgc
     2221 ttatccacgg aatctgtggg taactttgta tgtgtccgca gcgcccgccg cagtctcacg
     2281 cccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg
     2341 tcaccggacg cgctgatgtg ggatattgtc ccctgattaa gtctttctat tccctgacga
     2401 tcaattatca ggataggtga accatgtcgg tttttcgctg aacattttac gttaacctat
     2461 ctctgttgct tttttctcct tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga
     2521 cttcagggga aaagaccttg cttacgctgg ctggacgcca ccgacgcacg gtcttttccc
     2581 ctctttctgg ctctgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc
     2641 aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctga cgaagcagca
     2701 aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc
     2761 ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc
     2821 tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgcg
     2881 gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc
     2941 cgcggcttgt gcagcggggg ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga
     3001 gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc atatcccgga gctggcccgg
     3061 cagatttacc cgtgggtcag ctggctgtac cgggagtatt acagtctggc cggttttttt
     3121 ctggttatcg gcctgttttt cggtctggct gaactctggg ccggccgaat gcgttttgtg
     3181 gggctgagcc ataaggcgcg gcgggagtat gccgatcgct ataagcgcga taataaccgg
     3241 actgtgccat tttcggtgct ggcctcgctg gattcacccg ggcggatggt cagggcacgg
     3301 cgacatgtct ggaactacgc gtactggctg accggcgctg ccggtctggc ccttctgggg
     3361 gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg cccggacctc
     3421 tatgcgaacg cagattatta cctgctgcag ccggctaacc tgttcgggat agcctggtgt
     3481 gccggagggc ttgtcgccgg cgtccttctg gcgcggctgc tggcgttcct gctgcttgat
     3541 ggtcattttg cggccgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc
     3601 agcagccagc ggaccggaga aatgacagac gtccgccacc tgcatttcgg ggagccggta
     3661 ccggttaacg cgctggccga tttttccacg gagcaggccc ggaaacagca ggcggtgttt
     3721 ctgggtaagg atgagcaggg ccagccggtg ctggtccctc gtgatacctg gcgcaagacc
     3781 aatattcaga tcctcggcct gcccgggagc ggcaagagcg cgatggccac caacgcgctc
     3841 attcgctgcg tacgcgattt cggggacgct gtggtgtact tcgaccccaa aggggacgcg
     3901 tgggcgccgc acgtcttccg ggcgcactgc ccggacttca ccctgctgga tctgcggccg
     3961 gggaaaccgg cgcagctgaa tctgttccgc gatctcgacc agtacgccct gaagaatctg
     4021 ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc
     4081 gagcagaaag cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag
     4141 gtgctggcgg cggcgtacgc gttgccggag gccctgaaaa aggacgtgaa ggggctgatc
     4201 accaagctgg agaacgtggc cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg
     4261 gcggggataa tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg
     4321 gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat
     4381 gagttccgga ggtggccgca cgcgagcatc atgctcgatg agattaaata tctgctgtcg
     4441 aagtacgtgc tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg
     4501 caccagtcgc tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa
     4561 accacggtac tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc
     4621 gcgcagtggg cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc
     4681 agccgggagg cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg
     4741 cggccgctgt ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc
     4801 gggctgggcg tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt
     4861 tcgctgaaaa ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg
     4921 gccgacggcg gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg
     4981 agcagtacaa cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga
     5041 ccctgcgctg gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg
     5101 ggattgcggc ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc
     5161 tggtccgggc gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc
     5221 acggggtggc gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc
     5281 cgtcaagggt gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct
     5341 ccatggaggg ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga
     5401 tgaaagtgcc ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc
     5461 gcaccgtcaa gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc
     5521 gggcgaacgg catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg
     5581 tacagcgggc gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca
     5641 aaacggtgga gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt
     5701 gagggattat ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc
     5761 gctgcattat gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg
     5821 gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg
     5881 ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa
     5941 atccgcaatg ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa
     6001 aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac
     6061 cggagcagat taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga
     6121 acgagaagct gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg
     6181 tgcctgaact gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac
     6241 gccagcaccg gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc
     6301 agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca
     6361 gctcgttcag cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga
     6421 gggcagtttt ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac
     6481 cagccggacc agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg
     6541 gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta
     6601 tcttccagca tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga
     6661 agctgttcct gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt
     6721 ttctcaggga tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg
     6781 ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt
     6841 cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact
     6901 gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc
     6961 tgaattttac ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac
     7021 cggcgacggc cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc
     7081 cggatacccg gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg
     7141 gtcgtggcgg tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata
     7201 ttccagcggc agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat
     7261 agcccggacg atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa
     7321 gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc
     7381 ggagcatcct gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca
     7441 gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac
     7501 tggctgcgct tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc
     7561 gctgagcggt gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc
     7621 ctgatggatg ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact
     7681 actttaatcc gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc
     7741 ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct
     7801 gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact
     7861 ggtcgcggat cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc
     7921 attgccgggt gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa
     7981 aaaaggtgac agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac
     8041 gtggcggtca cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc
     8101 agtgtggtta cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc
     8161 actgtcagag taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc
     8221 aggtattttc atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt
     8281 tacagagttt cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta
     8341 tggatatccg cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt
     8401 caacagcggt cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat
     8461 aaattttcgg cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca
     8521 atgccagcag gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt
     8581 tacatgtata tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta
     8641 tcgtttttac accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca
     8701 atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag
     8761 ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata
     8821 ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct
     8881 cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc
     8941 tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg
     9001 ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca
     9061 ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct
     9121 ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa
     9181 tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt
     9241 acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca
     9301 cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt atgttggctt
     9361 taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt
     9421 ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg
     9481 tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg
     9541 ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga gtttggtaac
     9601 ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt
     9661 ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg
     9721 gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg
     9781 ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg
     9841 ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta
     9901 ccggccgatg cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt
     9961 cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc
    10021 gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac
//
