LOCUS       NZ_CP065942             2495 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain MSB1_8J plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP065942
VERSION     NZ_CP065942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17054651
            Assembly: GCF_016126855.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wyres,K.L., Hawkey,J., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Antibacterial resistant infections in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wyres,K.L., Hawkey,J., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:33:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,083
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,856
            CDSs (with protein)               :: 4,856
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 40 of 103
            Pseudo Genes (incomplete)         :: 72 of 103
            Pseudo Genes (internal stop)      :: 21 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065942.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MSB1_8J"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2014"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            790..1017
                     /locus_tag="JDC97_RS25295"
                     /old_locus_tag="JDC97_25295"
     CDS             790..1017
                     /locus_tag="JDC97_RS25295"
                     /old_locus_tag="JDC97_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1383..1568
                     /locus_tag="JDC97_RS25300"
                     /old_locus_tag="JDC97_25300"
     CDS             1383..1568
                     /locus_tag="JDC97_RS25300"
                     /old_locus_tag="JDC97_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1859..2077)
                     /locus_tag="JDC97_RS25305"
                     /old_locus_tag="JDC97_25305"
     CDS             complement(1859..2077)
                     /locus_tag="JDC97_RS25305"
                     /old_locus_tag="JDC97_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1616..1703)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          49..555
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
     Typing          2330..2495
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.31"
                     /coverage="99.0"
                     /evalue="5.56e-43"
ORIGIN
        1 aggctccgcc cccctgacaa gcatcacaga atctgacgct caaatcagtg gtggcgaaac
       61 ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca
      121 ttcctgcctt tcggtttacc ggtgtcactc cgctgttatg gccgcggttt ctcattccac
      181 gcctgacact cagttccggg aaggcagttc gctccaggat ggactgtatg cacgaacccc
      241 ccgttcagtc cgaccgctgc gccttatccg gtaactatca acttgagtcc aacccggaaa
      301 gacacgacta atcgccattg gcaacggcca ttggcaaaca ggatgtgcga gagatgtctc
      361 aagagttctt gaagtggcgg ccttagtgag gctacactga aaggacaaat ttggtgacat
      421 gtctcgcaca agactgttac cacggttagg ggatctgcca ggattaccta accttcgaaa
      481 aaccacctgc cagggtggtt ttttcgttta cagggcaaga gattacgcgc ggaaaaaaag
      541 gatctcaaga agatccttta taaattagac atccaacctc acaaagagga atacttaaat
      601 catcttggta tatgtatcgg ccggattaca tctcatataa gcctcctccc cgcctgacac
      661 cacactttga ccatagcacc tcgcgcctaa ctcgatatac agcccaccat caagcctctt
      721 ttcacataaa aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag
      781 gagctggctt tgaaaaagat ttttgtttca gtgttcgcag cggcagttgc gctttcagcc
      841 ctcaccggct gcacgcgcac aagctatgcc atacacacca atgacggtcg cactattgtc
      901 agcgacggca aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt
      961 gtcaaacagc agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca
     1021 tcttctccga aaaaggccgc taatgtggcc tttttcttta cctgtggttc tccgccaaaa
     1081 tcccagcaaa ttgcatcacc aaagctaaaa gctttcaggg ttgtctaaaa agcacaagac
     1141 ataagaggaa gtgcggtatt ttataatcaa gcccccaaga atttaccata acatccgttg
     1201 cccgcaccgc ctgagacgcg ttcagcgcgt tcctgacgaa accatgacaa aaaccacaac
     1261 aaaccacccc ggaacccgtc agaaacgcgc ctgttaaatt ttaacggcat gcatgactat
     1321 gcaccagaat gacgccatgc tcttttcacg caaaaatcat caccagacgg ggaaaatcac
     1381 cagtgaccag acaggaatcc gccgccctca atatggccaa atttatccgc gcacagacac
     1441 ttctcctcct tgagcggctc gagcagatgg atctggatga ggctgccggc tgctgtgagc
     1501 acctgcacga tcaggccgaa gcgctttacg ccatgctgaa cgcacagata ggcgaggaaa
     1561 atgcgtgaaa atcggtgaac gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg
     1621 gtgtcggggc gcagccctga ccagggtatt tgtgatgccg gcgcgtgcgc ggtattacaa
     1681 atgcacatcc tgtcccggaa cggacaccgg gaaacagcaa aaaaaaccgg gcggcacgcc
     1741 cggaactcaa tcaagttaga ttagattact ctcactcgtc cataacagca tcatggaacg
     1801 acgaccaccg tccgtgacgg ccgcctcgtt taagtatgga cagaaataca gaaaatgctc
     1861 aggacgaaat gtaatgaatg cgaacggatt caagaaattc gagcatgaca gtccttacgg
     1921 ccggttcggt ttcagacaaa atctgccggt atgcatccag catcatggct ccggcatccc
     1981 ctccggcacg ccgtagccag accgaaacaa cggacacaag caggtgtcgc tcatcatcac
     2041 taagagtcat cagggctccg gaagaaaaac caaacatggc ttacctactg ttaacggcaa
     2101 cgcagaagaa aggtttacag ggtagcgggc agggatgaaa aaaaaaaaat tgcagtcagg
     2161 ccggaacggc tgctgaaccg gtacaggcgg gttccgggcg cagcgccgga ccagtcacgg
     2221 agcactagcg gagtgtatac tggcttaaga tgtagcatgg cagtaacaca tagcattaat
     2281 gccacataag acaggcgcag aaagtgaaag aaaacgcgtc aggagaaaat gtgatacaga
     2341 atatattccg cttcctcgct cactgactcg ctccgctcgg tcgttcggct gcggcgagcg
     2401 ggacgggctt acggatggat cagaaatttc ctgggggatg ccaggaagat acttaacgaa
     2461 gaaagcataa ggccgcgcca aagccgtttt tccat
//
