LOCUS       NZ_CP065941             4853 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain MSB1_8J plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP065941
VERSION     NZ_CP065941.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17054651
            Assembly: GCF_016126855.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Wyres,K.L., Hawkey,J., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Antibacterial resistant infections in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Wyres,K.L., Hawkey,J., Judd,L.M., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:33:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,083
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,856
            CDSs (with protein)               :: 4,856
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 40 of 103
            Pseudo Genes (incomplete)         :: 72 of 103
            Pseudo Genes (internal stop)      :: 21 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065941.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MSB1_8J"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2014"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            complement(join(4782..4853,1..357))
                     /locus_tag="JDC97_RS25260"
                     /old_locus_tag="JDC97_25260"
     CDS             complement(join(4782..4853,1..357))
                     /locus_tag="JDC97_RS25260"
                     /old_locus_tag="JDC97_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            642..1070
                     /locus_tag="JDC97_RS25265"
                     /old_locus_tag="JDC97_25265"
     CDS             642..1070
                     /locus_tag="JDC97_RS25265"
                     /old_locus_tag="JDC97_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1127..1618)
                     /locus_tag="JDC97_RS25270"
                     /old_locus_tag="JDC97_25270"
     CDS             complement(1127..1618)
                     /locus_tag="JDC97_RS25270"
                     /old_locus_tag="JDC97_25270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127627441.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIQP
                     "
                     /name="hypothetical protein"
     gene            complement(2082..2297)
                     /locus_tag="JDC97_RS25275"
                     /old_locus_tag="JDC97_25275"
     CDS             complement(2082..2297)
                     /locus_tag="JDC97_RS25275"
                     /old_locus_tag="JDC97_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2304..2795)
                     /locus_tag="JDC97_RS25280"
                     /old_locus_tag="JDC97_25280"
     CDS             complement(2304..2795)
                     /locus_tag="JDC97_RS25280"
                     /old_locus_tag="JDC97_25280"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2830..3534)
                     /locus_tag="JDC97_RS25670"
                     /old_locus_tag="JDC97_25285"
                     /pseudo=""
     CDS             complement(<2830..3534)
                     /locus_tag="JDC97_RS25670"
                     /old_locus_tag="JDC97_25285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3473..3796)
                     /locus_tag="JDC97_RS25290"
                     /old_locus_tag="JDC97_25290"
     CDS             complement(3473..3796)
                     /locus_tag="JDC97_RS25290"
                     /old_locus_tag="JDC97_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015060398.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3912..4085)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          4009..4555
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1985..3483)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3480..3796)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="1.6e-61"
ORIGIN
        1 tagacgttgg aagtggatga cccaatcaga gactttgtac ttcccgtgaa atgtatcgat
       61 gaaaacggga tcgcctatat gggcatgcca caatttatta gccctggcat gttcttcaat
      121 agcttctttg ttttcgtaac tgtcaggcac gttccaagat ggcgatacct tgccaatcca
      181 agccggatgg ccttttaagg catcgccaaa aagattgtac ttctcgaaat gttcgatgaa
      241 gtcatcgatt ttatcgagtg ttttttgaga aaagcgggat gtgtaataaa gattgaatgt
      301 ctcagagaaa gagacaacat tcaattgtgg attttgcgtg cttgatccgc tgcccatgct
      361 cttctctgct ccctacttag accagatggc attgtaacag atctctcatt cgctctggaa
      421 atcaacagag acaaggatac accaaatatt tcagtatctt ttacaagatc atgatgttgt
      481 ttggttttca tatcactaac tccgtattta atgcagatag aaatgttttt ctgttctcag
      541 attagccttt tatagttgtg taataatggc gctggtgtca ccctattcat atgaatactt
      601 atattatata tatctcaaaa aaaatattta caggataatc aatgagttac gcaatagaaa
      661 aaaaatggaa ggctggaggt ctgtcattag gatggactgc agtacctact gtgttgtttt
      721 ttatacaaaa tgagaagaaa ttgagccctg tagcattcaa taccttgctg aatctcatag
      781 tacactggtg gaatctacag gagtggccgc acccctcaat ggagagcctg gctatacgga
      841 tgggggtgtc agtaaggacg gtgcaaaggg ctattaatga cctagaaaaa gccaatctct
      901 tagataaaaa accgacatca aaaagcgaca accgatatgg tggccgcaac atatacgacc
      961 ttacaaagct tgtagattat ttagacataa ttggtccttc tgtgtccgag caagtaagaa
     1021 agccgcgaca taaaaagcca atatatacag taaggaaaaa agccgattag ttatgctggt
     1081 tacatgcagg aacagcccgc atttggaggc cgggcttatc gttaaattag ggctgaatcg
     1141 taagaccaga acgttgaatg tattcatcaa cgcgatcgcc aatgtttaca attttggcga
     1201 aattcacatc agccgatgac acgtttagat ccgttgcgga tgcaggcata ggcaaggttg
     1261 cacgatgacc atcgacggaa accaaggttt ttctatcaac aaacgaagaa ccatagtgaa
     1321 cggtgtactc gactgaacac gcattactat caggatgacg aacagcccaa ggctcattaa
     1381 acgagttgta gtcgtcgtaa tcagctctct taatgtgcag tgctaaatca tttttataag
     1441 taaacgcccc tgtctcatca tccacaatcc aatcattttg actcgattgc gcaagaattc
     1501 gtgtaacttc atcagcattc ataaatagct ccatcaggca gtgtgtagat aaaaagcaaa
     1561 caacatcacc tacatgggta ttatcagaac ctttatcaac ggacggttga tttaccataa
     1621 catctgttgc ccgcacagct gaaacggggt tggctgcgtt tccggtgcca gacgcgagaa
     1681 agtggcgaca gacatgacga aaaaacaccg gaaacgcagc cgctggatat tacacttatg
     1741 catagtcatg cagccagatg catgccgcgt aaagtcacac taaaatcagc gcgaacgcct
     1801 taaaaaccgg caaatgcctc tccggcaact cacacagctc catcaccagg taaaaacagt
     1861 tcagcagggc ttcaataacg ttaagaattt tcatcacttc atccgggata agtcactacc
     1921 attccggtag tcagcctatt tagcaggaac gcgatgagcc cttcttcgtg cgggctgtca
     1981 cctcatcttc ggaccatcaa aactcttctg cttctgacgg agcgtcagct ccagctcgtt
     2041 ctgacgctga tactccagta cagccttctc ctcgatggca ttcagttccc ggacaaacgg
     2101 cgcagctgct cgcttaaact cagcacctgc gtgctcaagt ccgacacgcg ctcgttcagc
     2161 accgcgttct cccgttgcat aagcccacac atgctccgcc attccgcgaa ggcgctctcc
     2221 cattcgtcca gcctttgcga gtagtcctgc tgtagctgct ctaatgcgct cagcaactgc
     2281 ttttccagct ctgtcatctt ccgctactcc tgtttcaggc ggatccatgt cgtcccgtcg
     2341 taagggatgg cttcggtttg catccccggc ggcaggataa gaaacctccg gccgtcgctg
     2401 ctctcctgat agcgcactcc ccacgtccgg gcggtcattt tcgccagggt atcttcctgc
     2461 tgacgaagat gcgtgtaatt gtcggttatc tgctgcccct gccaccacag aatgctcccg
     2521 ctcgtgccga tcagcagcgc tgacaccatc aggatggtga gccatgtccg cccggcagtc
     2581 cgcagtacgc tccgccggtt tttctccatc gcctcgctca tactctgctc gtgggcgagg
     2641 atggcgtcgc tgattctcct cgcgcttgcg ctcagttccg ctctgacaga ctgctcgtgc
     2701 tcgctgaatg cggctttcag catctcgccg gtactctgct tctgcgcttt cgattgctgc
     2761 tctaagtcct tcgccagcgt taaaagactg ctcatagatg gctcccttca gtcggatgtt
     2821 tcgccccccg tccgggtcgg caatgctgat actgcttttc gtggtgcgca ccacctcaaa
     2881 acctgaccgt tccagcgcct cagtgacatc ctgacggctt ttcagctccc cggacgaagc
     2941 aagggtcagc agtccacgcg tgatggcttc tgcggcctcc tgcttcgttt tcggcagtcc
     3001 ggacggcgtg accagcgccc ggcggttttc cggcgcgttc gggtcatgca gccccagtct
     3061 gccgttcacg atggtctgcc aggcatcgat gcgcggccgg tcggcgcggt cgtagtacgg
     3121 ctggagccgc ctgccggtca gcagctcggt attggggatc agaaagttca gctccagacg
     3181 ccccttgtcc gcgtgctcca cccacagaat gctgtactga tctttatcaa gtccgggcat
     3241 cagaacccgc tcgaagctgg ccatcagttt ttcccgctgg ccgggaggca gatcggcctc
     3301 cgcaaacgac aggacgccgg aagtgtattt cttcacgtaa ggcgacgcgt caatcagctc
     3361 ccggacttct tccggcttcc cctgcaggac ggtggcacca tcacgctgcc ggtctttacc
     3421 cagcaggtag tccaccggcc cggcgccacc gccgcgaccg cgtggatgaa atttaacgat
     3481 catcgtccgt tcctttctca agaacggcgt gccgcagccg ctcaagtccg gcatcgatgg
     3541 ccatcagcgc cgccacaatc tgcaccctgt catggccagt gccgccgccg gcgttaacgc
     3601 ggcgggctat ctggttcagg ttattgccca taccggcaag ctggcgcagc agcgccggcg
     3661 agagtgaggg gagctttccg gaacgtgccg gcttttcgtc caggcaggtc tgccgcatcc
     3721 acgctgccag ctgcctgccg tcgcagcgct caagcagccg ccggtgctcg tcctccgtca
     3781 cccacatggt gagcatcttg ttccgtttct ctgacaaacc ggcctcctga taacgccggg
     3841 acgggcggga aagcctttcc cggtcggcgc ggggttggac aagccgcagg cgcgtcagtg
     3901 gcttttagcg ggtttcgggg cgcagccctg aaccagtcac gcagcgctag cggagtgtat
     3961 actggcttac tatgttggca cgggtgcggg aatcagtgaa gtgcttcatg tggcaggaga
     4021 aaaaaggcgg caccggcgcg taagcagaat atgcgataca ggatatattc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgcgggc ttacgaatgg
     4141 ggcggaaatt tcctggtaga tgccaggaag aaacttaaca gggaaacgat aaggccgcgg
     4201 cgaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     4261 atcagtggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     4321 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     4381 gcgtttgtct cattccacgc ctgacactca gttccgggaa ggcagttcgc tccaagctgg
     4441 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcaac
     4501 ttgagtccaa tccggaaaaa cacgacaaag cgccactggc agcagccact ggtaacaggt
     4561 ttagaggagg agagtcttga agtttcgggt gcgataaggc tacactgaaa ggacagtttt
     4621 ggtgactgtg ctcctccaag ccagttaccg cggttcaaaa gagcgtcaac tcttgcgaac
     4681 cttcgaaaaa gccaccttgc cgggtggttt tttcgttttc accgcaagag attacgacga
     4741 gggtcaacga atctcaaggg atcattcatc cgaacaagag atcattcgaa attttggggg
     4801 agttgcatag ggtcatgata cccaagctct aacagtttta tatggttcgg cgc
//
