LOCUS       NZ_CP065933             9316 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Enterococcus faecium strain Dallas 5 plasmid p5_9kb, complete
            sequence.
ACCESSION   NZ_CP065933
VERSION     NZ_CP065933.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17038066
            Assembly: GCF_016126675.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Islam,M.J. and Palmer,K.
  TITLE     Molecular characterization of VREfm isolates from Dallas, Texas
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Islam,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 1520 Richardson Dr Apt 1418, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:26:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,938
            CDSs (with protein)               :: 2,938
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 42 of 115
            Pseudo Genes (incomplete)         :: 64 of 115
            Pseudo Genes (internal stop)      :: 31 of 115
            Pseudo Genes (multiple problems)  :: 18 of 115
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065933.1.
            Bacteria and source DNA available from Dr. Kelli Palmer, UTDallas.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Dallas 5"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p5_9kb"
                     /geo_loc_name="USA: Dallas"
                     /collection_date="2015"
     gene            complement(285..455)
                     /locus_tag="JCP67_RS15495"
                     /old_locus_tag="JCP67_15495"
     CDS             complement(285..455)
                     /locus_tag="JCP67_RS15495"
                     /old_locus_tag="JCP67_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(471..1010)
                     /locus_tag="JCP67_RS15500"
                     /old_locus_tag="JCP67_15500"
     CDS             complement(471..1010)
                     /locus_tag="JCP67_RS15500"
                     /old_locus_tag="JCP67_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1038..1775)
                     /locus_tag="JCP67_RS15505"
                     /old_locus_tag="JCP67_15505"
     CDS             complement(1038..1775)
                     /locus_tag="JCP67_RS15505"
                     /old_locus_tag="JCP67_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            2420..2506
                     /locus_tag="JCP67_RS15510"
                     /old_locus_tag="JCP67_15510"
     CDS             2420..2506
                     /locus_tag="JCP67_RS15510"
                     /old_locus_tag="JCP67_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2626..3315)
                     /locus_tag="JCP67_RS15515"
                     /old_locus_tag="JCP67_15515"
                     /pseudo=""
     CDS             complement(2626..3315)
                     /locus_tag="JCP67_RS15515"
                     /old_locus_tag="JCP67_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3317..3595)
                     /locus_tag="JCP67_RS15955"
     CDS             complement(3317..3595)
                     /locus_tag="JCP67_RS15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(3555..4469)
                     /locus_tag="JCP67_RS15520"
                     /old_locus_tag="JCP67_15520"
     CDS             complement(3555..4469)
                     /locus_tag="JCP67_RS15520"
                     /old_locus_tag="JCP67_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4451..4831)
                     /gene="mobC"
                     /locus_tag="JCP67_RS15525"
                     /old_locus_tag="JCP67_15525"
     CDS             complement(4451..4831)
                     /gene="mobC"
                     /locus_tag="JCP67_RS15525"
                     /old_locus_tag="JCP67_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(5273..5410)
                     /locus_tag="JCP67_RS15530"
                     /old_locus_tag="JCP67_15530"
     CDS             complement(5273..5410)
                     /locus_tag="JCP67_RS15530"
                     /old_locus_tag="JCP67_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(5524..6144)
                     /locus_tag="JCP67_RS15535"
                     /old_locus_tag="JCP67_15535"
     CDS             complement(5524..6144)
                     /locus_tag="JCP67_RS15535"
                     /old_locus_tag="JCP67_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(6141..8267)
                     /locus_tag="JCP67_RS15540"
                     /old_locus_tag="JCP67_15540"
     CDS             complement(6141..8267)
                     /locus_tag="JCP67_RS15540"
                     /old_locus_tag="JCP67_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(8321..8695)
                     /locus_tag="JCP67_RS15545"
                     /old_locus_tag="JCP67_15545"
     CDS             complement(8321..8695)
                     /locus_tag="JCP67_RS15545"
                     /old_locus_tag="JCP67_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8828..9289
                     /locus_tag="JCP67_RS15550"
                     /old_locus_tag="JCP67_15550"
     CDS             8828..9289
                     /locus_tag="JCP67_RS15550"
                     /old_locus_tag="JCP67_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     BGC             3321..9316
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1039..1775)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3559..4469)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagttgacat aatgtacact atgccaagta gcccctctac caatggatag agacgctcac
       61 tttgtgattt ttaattggtc tagctaaaag caactttcta aagcttgatt tttcgcggtt
      121 atctatatct aattctttgt gcatttttat ctgtcatttt tttaagactt gttatttttt
      181 gtaaaatatg gtaaattttt ggtagcgctt cataagcgcg tgctttttca agaataaaca
      241 ctcattattt tttaactggc tgctcggaag agcagtcttt tttattattt atccgtctct
      301 tttacaaaca attcttcgtt aggactgttg taataatttg gattcaattt caattcacca
      361 tgtttattcc ctttattgat tactgacaat aacatcggac tgaagcctag aattcctagt
      421 aaaatagaac cgaatagaag attttttctt gtcatgaatg ttccctccag ttatttccaa
      481 agttgccacc atttttgctt cttctttttt gtgtgttttt ctatcgattc tgtttcttca
      541 gaaagaatag tagttgattc atcttctgga gtttcttccg tataagctaa ttgtaactgt
      601 tcttgcagtt tttcgatctg ttgatttgcc tgtagggtga gctgttgttg ctggtcgagt
      661 aatttttgca tattagaatt attttgctct ttgagggaaa gttgttctcg taaaaattca
      721 ttttcttcac gcaacttatc tagcaactcg tttacaatca cgtttttgct tgatatataa
      781 acgtttttat cgttttcttg ttcatactca tgcaacctat atagtaacct ttggttacct
      841 aagttgctag caagttgcaa tactccagaa cgtaatacaa ccttataacc acgctcattt
      901 ttagctaata aggacgggtc taatttatca acatgtttag agattgcttg cctacttatt
      961 tcaaataatt cagataattc ctttattgta aaggtttctt tgggttgcat gataattgcc
     1021 tcctaaattg ttaggtatta tttctctaac caattatcta aggtaattga aggtatattt
     1081 tcttcaaaat aaatttgcag agatcctatc ttatttcctt tgtaggcttt aatttttttg
     1141 atttcaaaag ttttaaaaat agcagggtga gttgaattaa cgtttaaaaa ttcttttttc
     1201 gcttgggcaa aaatttgttt atcaatatct ctcatgcgat aactttctgg tattgctaat
     1261 agagctctaa aatcttctat ttttacaaac cagtgacctt ttttagatct tctgtcttta
     1321 tgagccataa gttggcgata taactcttta acgtagctac tctttaagtt tgtcatattt
     1381 tctaattcga aacgagtaaa attagttccg attgaattaa gtatatattc aaattcttca
     1441 gaaaccttta ttaataatgt ttcattcact ttatcgattt taaatttttg aaacagattt
     1501 aattgttcat atgttttttc gtcttcataa ataaaagaca aagtaatgat tttttttgac
     1561 atttttatta aatcattagc aaaacggtcg ccacgtgcat ttatattgta atcaattaat
     1621 tcttttattt gtttaaatgt aaaaatttct tcttttgtac cttttctttt cattttagaa
     1681 catattcccc aaaataaatc catatctata ggggtaaatt ttcttaaagg gacagaattt
     1741 aatccattat tatatcgaac tatatcgtta ctcaataaaa atcacctcaa taaattatgt
     1801 tatcatagac ataagaggat gtaaaacaca atttatcccc catagtgtga caatttatcc
     1861 cccatagtgt gacaatttat cccccatagt gtgacaattt atcccccata gtgtgacaat
     1921 ttatccccca ctacctatgt caatacttac tctcccataa ggttgctaat ccctaacagt
     1981 tattattaac agtatatcta acagtatatc taacagtata tcttaagagc acattgaatt
     2041 tcccgcctaa aaaactccta ctttttcctc caaaaaggtt cgcgaatttt tctaaactgg
     2101 aaaacttgct ccctacggtc gctaaaaagg tgtgatcgct atgctctcat attatactgc
     2161 gcagtcgcct ccggctcctt tgctaaaacc gttgttcaaa gaggaggtgt gagactctga
     2221 aagcttgtct aagccgttct aagacaattc ttgattaatt tcccttaatc tagtcgagaa
     2281 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aacaagtgat acttaaaaac
     2341 accacacaaa tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     2401 ctggaaggag gtgtatctaa tgtttgaact gttcatttgc ccccttcttg taggagtttg
     2461 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     2521 acatcaacct gaaaaaagcc ccagagaatc cgacctctct ggggctttcg tgtatctaat
     2581 gtttgatcat ttgcacattc agtttaacat gattttaaat caaattcaat tttgttgttg
     2641 ctgatctaac cattccttga cactttttag atagtttaag gcggtttctt tttttgtcgg
     2701 attttgcatg ttagttgctt gttgaagtaa gatttcatag cgttcatatg tgacagattg
     2761 ttgtttttct tttttttctt cttcactggc aaaccaaggg tttgtgtctc caaatagatc
     2821 actacaggct tcttctatct ttgtatcttt tacatattgc acgtcttttt tggttcgctc
     2881 atagttgtct tgaacctctt tttgctgttt ttctggcaag ttttgataaa gacgagtgag
     2941 tttttccatg tttttatccg tcaaacgttt ttcttgattc acaaaaatat cgttcatttc
     3001 ctcataacaa gtggtaaact cttccatttt ttgtgctaat gcttgctctt ttttcttttt
     3061 atcacgcgct gattcatact tttgagtgac atttcctaat aaaatacttc tagaaacgcg
     3121 cttatttccc tctaaaacgc cgaataagcc gcttgaaacg attatcaagg aaaatataca
     3181 gccaagaaag aaaaatcgct taaatcgaat tttagagcct tttaatgaat atcttgttta
     3241 aaaaatttta tagatgagcc aaatacttcc tgaaatacta acaaaaatga gtagataggg
     3301 taaataaaat gccattttag cctccccata ctaaccaaaa tgcgaacagt gtcaggacta
     3361 agcaacataa gggggcaaga aaatccaatg atcgttgaat aagcaccttt ttctgttcct
     3421 tatttcgctg gtctaatagc tcttgatgga gttttttaac ttgtaaatca atcgtagttt
     3481 gtatcctttc ttgcgtataa ggaacgagaa aagcacgttg tttctttatt tcgttttcta
     3541 tgtcttttaa cgttttagag cgataattct tgttggtggt ctgttcctct ttcaacgtgt
     3601 tgttcatggt ttcttcgagc cggtctaatt tccggttgtt caggcgtaag agttcgttgt
     3661 tctcttcgta cttcttctgc agtttcgcga atgctagctc gagctcttcg tagctcgttg
     3721 gttcgctgtt ggttataggt tcgttctcgt ctgtccagct catgtaaaat cgtctccttt
     3781 tcgtagtcct ttcctagtct gttggatcgt acttttttct ttttgtggat caattcgtaa
     3841 gtgaaatttt tcccgcgatt gaagcaatga atccccaatg ttttgaggga attacaaaat
     3901 tggtcatagg ttctggtttt tttgtttttc attgtttgat cgatcgcaat ccgaatttcg
     3961 tctttccaaa caggttcatt tttttctttt aattttattt cagacatggt tcgtttttct
     4021 ggtttctttc ctcgctcagt gattggtaag ttatgtgcaa gacaaatctc gtcatttagg
     4081 tcataacatt tgtttagaaa tgcttgacca tgagcttgaa atttttttcc agtctccaga
     4141 ttaacggagt taatgacaat gtgattatga atatggtctt tgtctgtatg tgtgtaaata
     4201 gctatttggt gatcgggggc tatttttttt gcatactctg cccctagttg attagctttt
     4261 tcagcggtaa tttctcctgg tcgaaaagat tgaatcaata agtgtgcctg tacacggtcg
     4321 tcttttccat acagcatgcg aatttgtttc atttcacttt tggcataatc gacgtcacag
     4381 tttattcctg ttttaatggt tgctcgtggt tctgcgtaat taattgcacg actacaagaa
     4441 gtggttcggc ttaccttaat tgttgccata tcttttgtag ctccttttgc atgattttta
     4501 ggttttcgga taaacgttga gcgtcaccag aagcaacgtc atgttggtgt gtattgcacc
     4561 acttagcaat ttgattgata ttccctccag ctttggctaa atcgcgcgcg attgcctggg
     4621 caatgtctgg cgccactttc ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga
     4681 aagccggcac actcatttgc aaagcttcag ccgattgctt cagctttgca aattcggatt
     4741 cgctcactcg aaaactgatt tgttttggct cggatcgata ggtatatttt ttagaagaat
     4801 tgctagccaa attttgattt tgttcgctca taagtcccac cctttcagta cgtcctacgg
     4861 acgtatcgtt cgcaatacca ttgtacctaa aaaggtacaa tgagtaaagc tcactaaaag
     4921 ggtttggcaa gctctatatt tgctagccaa atacctacgt atttagtgca aataaccgct
     4981 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcaaa agccacgctc cacaatacaa
     5041 aaaaagctag caaatgctag ccactttttg taaagtttcg cttatttcac cgaagatgaa
     5101 attgttgctt tgcaacaaaa aacataaatg cttttttgtg taaactacta actatttagc
     5161 taaaactgat agttttatta aacattcagc aatattttta gccaattttt tatcagactt
     5221 tcggcgagtc attatgataa tatgtccact ttttgttact tctaccaaaa atctagcaat
     5281 aactctccca ttcttcagtt gatttagttt cataagtctt ttgtaattga cggtttaatt
     5341 tttttggcaa ttgcaaagaa taaataagtc tatcataaaa tttttcatac tcttttttca
     5401 ataaaatcac ttgggtgcct cctttttatt aagtgattat ttaaattttg ggataggaga
     5461 gatggaatat aaaataaata cccgtggtgc tagttttaaa tcagctagca tcacgggtaa
     5521 aaattataat tctataatat tagttccata gtttagaaca tttaagtcat gtgttactag
     5581 tacaacggct tttttttctt tttctgttaa atttttcaga aagttcatta taaaagttga
     5641 gttttcttta tctaaatttc ctgtaggttc atctgcaaat aatatatcaa atggttttaa
     5701 catatttcta attaatgcta ttctttgttg ttctccaccg cttaagagaa agattttttc
     5761 ttttaagata tcttttggca aagaaaaatc tgttaataaa ttataaatat gatcattgga
     5821 aatattactg ttaaactttt ttgctaattc gaaattgtat tgtactgttt gattttcaag
     5881 aagagcatag ttttgaaata aatagctaat atgatttctt cgtagacgtt gtatatcatg
     5941 tttagaaact ttcttttcaa aatagaataa gttaccggta tattgtttat ctattaggct
     6001 taaattattt aacagagtag tttttccact accacttttt cctgttacta taataaaatc
     6061 tttttctgat attgaaaagt tccaattttg tttgatcttt tttttattaa ttgacagggt
     6121 gaaattctca actgataata tcataaaata ttctccttaa aatatttaaa actatttttt
     6181 ttataaataa taatagaagt aattagattt attaaaaaga tcataaaaat tataaaatac
     6241 aagtaagtgt tttttgaaaa tatcaattga aaacacatag tagcaaataa ctctattgta
     6301 aaagggagta aacataaatc taaaacttga cttattggtg aatatccaaa tatttttttt
     6361 aaagctattt tttttgaatt tatagtaaaa ctaacaaaaa tcatttgaaa attaattatt
     6421 ataaataatc ctaatattaa aagatattgt agatatgcat ttaatttatt tacttttttt
     6481 tgagataata attcaccagt ttcttgaaaa acagaataag aaaaatttat gatattttct
     6541 aggttaaatt cttttatata cggattaatt tgattaaaaa agttttggtt tgaagtatgt
     6601 ttctgatcaa aaaataatcc accatcacta gtcaatatat ttccccaaaa taatgattct
     6661 gttatttctg gattaataac tctaactatg ggatcagtta ttgtatagtt atcttttgcc
     6721 tttccatata aggtattaaa actaaaatac ttttggttat tattaacaaa tattagtttt
     6781 atttttaaat ttttcaaatt aggattttta taaggagctt gaggaggaga gtcctcaagt
     6841 ctaaactgta tgtcttctaa ataatttttt tcaatcattt ttgcatattc tttatatttt
     6901 ttaggtacta gaatgtaagt agttagtgga tcagagttta ttaaatttaa gtcagtagat
     6961 tcttcaaaag aaataggatt gtaatatttt agatagttta aatcaacaca tattcctgca
     7021 ccagcaggtt gactaacata gtcttcaaaa tttggataaa ctttttgtcc aataaaaaat
     7081 tctttattat tctcttggat agttgcgtaa ttgtatggcg cgataataaa agttttataa
     7141 ttcttctgta ttttataaac aaatttacta gcttttttca agtagttatt ttcttctacg
     7201 gtgttgtttc tatttaattg attagtaata tttgttttgt ataaattagt agttctttcc
     7261 caatatttaa gagaattatt ttcggcttca ttatgggtat ttattacttt tgtagaagct
     7321 attaacagaa ggatcatgct taagaataga cagatattta atatacaaag atttaactta
     7381 agtggtcgtg aattttttag ataatttttt atactagata aatgatagat acttagagta
     7441 actatgataa ataaaaaact aattataaaa tgaaaaatac tacatagaag aaatgatagg
     7501 aaaaaatttt cgttactgta atatatgctc aaaaaaatag ataaaatgaa agttataatc
     7561 aaagaacaaa ataagaggtt taactcttta atagaatctt ttatagtatc aaaagagtta
     7621 tagcctaaca ttttttttag agtgatttct ttcatttttg aaaaagcaaa tataattgta
     7681 gtaattacaa taaatatact aagatagcag ataacaagaa caatattagt attaatttga
     7741 aaagtatctg attgattgat attggaaata gtaggtgttc catatttttt cagtacagga
     7801 attaatttgt ttgagtttcc ttgtacatat aatatttcac ttaatccaat attttccaca
     7861 tgagaaaaag gataaatatt tatatttaaa aaattatttg gaagttttaa ttggtactta
     7921 gaatttttgt tattagtata atggacttta gaatcgtttc ttgctaatat atttttttta
     7981 gaaaatttcc agtgtttgtt agcgttaggg tttgtagcaa gaatagttag ttggttaaca
     8041 gcagtaaaac tatattgaga aatattaata ttttcttgtg tagatatttc atacaaagcg
     8101 tttatataac tgttaaagtc catatcatct ttatattgca tatgaatttc agcggtattt
     8161 ttatatagcg cttcagaagt ctttatacca tttataacat tagtagaaaa aaatagtatt
     8221 gtaatgcaga caaaaagtga taaaacagcg tttatttttt ttaacatatt ttgaatttcc
     8281 tttcaaatgt aaataaaaaa gagagtttaa actctctttt ttaataaaca ttccaccaac
     8341 cagtatttcc agtaagtgtg gcattgcatg ttgcataagc tcttactcca gctttctgaa
     8401 ctgatcgtac ttgaccgcct cgtccatttt tagctgttgc actatgttta gctctagcat
     8461 ggtagtaatt tgcataaact ttaaatccat taaccccaca gttgaaatcc ccgccgccag
     8521 cagcatatct caatttacta gaagaaaatc cttcttctgt agcttccatt cctcctccgt
     8581 aaatggcggt atggtcttcg ggtacatcta gatttaaagc ttctgtggca ctagctattc
     8641 ctccactagt taataagaca cccattaaag cagaaagaca taaaaatttt ttcataactt
     8701 atttctccta tcttttttac tagaattatt ttattttagt gtgttttata aaataaagaa
     8761 cttttttggc gcgttagtat ttttatgata aaattttatg taaataatcg tttacatagg
     8821 agaaaaaatg gaggatttag gatctaagct aagagaatta agagtgaaaa aagggtatac
     8881 tcaagaatat gtggcaagaa aatttaacgt tacacgtcaa actatttcaa agtgggaaaa
     8941 taacaaaaca acaccagata tagagacact aaaaaaaatt tgtgaatttt atagtactaa
     9001 cataaattct attttaaatg aagataattc aaaaaataaa aaaaataatt tactggtatt
     9061 gacagctatt atttttaata tatttgttac tatattaggt ttatttgaat tgattcaaat
     9121 gaattttttt agctttacac atggaatatt tacttggttt gctctatctg gattaatagt
     9181 aatagatatc ttaaattatt tttatattta ctattcttat ttaaaaaatg aaaaattttt
     9241 ttatacaatg tttttccccc tagttatttt gattatttta ttaagctaat atgaagtagt
     9301 tagttattta attgca
//
