LOCUS       NZ_CP065845             4000 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain KqPF9 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP065845
VERSION     NZ_CP065845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016746
            Assembly: GCF_016066695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 359x, 153x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:33:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,597
            CDSs (total)                      :: 5,474
            Genes (coding)                    :: 5,326
            CDSs (with protein)               :: 5,326
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 95 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 37 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KqPF9"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-05"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            complement(join(3842..4000,1..81))
                     /locus_tag="I8N79_RS27750"
                     /old_locus_tag="I8N79_27750"
     CDS             complement(join(3842..4000,1..81))
                     /locus_tag="I8N79_RS27750"
                     /old_locus_tag="I8N79_27750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153677472.1"
                     /translation="MKPGGAGLFKTQLPSTKNAIQLLADESKQATLRFPNLSFALFSHQ
                     FMWLADDFATQKKWKDKLLAFLATPIFSRVNQNI"
                     /name="hypothetical protein"
     gene            complement(446..559)
                     /locus_tag="I8N79_RS28290"
     CDS             complement(446..559)
                     /locus_tag="I8N79_RS28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_087796368.1"
                     /translation="MHEPPVQSDCCALSGNYRLDSNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            976..1167
                     /locus_tag="I8N79_RS27755"
                     /old_locus_tag="I8N79_27755"
     CDS             976..1167
                     /locus_tag="I8N79_RS27755"
                     /old_locus_tag="I8N79_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1167..1568
                     /locus_tag="I8N79_RS27760"
                     /old_locus_tag="I8N79_27760"
     CDS             1167..1568
                     /locus_tag="I8N79_RS27760"
                     /old_locus_tag="I8N79_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087796372.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHYGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2420..3184)
                     /locus_tag="I8N79_RS27765"
                     /old_locus_tag="I8N79_27765"
     CDS             complement(2420..3184)
                     /locus_tag="I8N79_RS27765"
                     /old_locus_tag="I8N79_27765"
                     /inference="COORDINATES: protein motif:HMM:NF017885.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="WP_087796371.1"
                     /translation="MTTPRLQAALVSEQWLRGKHFADALPVDCIKIAESLGIKVQGAPL
                     KDEFQGALFITPDVKAIIFNENIKEDGRKNFTIAHEIGHYCLHSDREEVQCRLEDMGAF
                     VLTPHGKEIEKEANEFAACLLMPEKDVQQQLLDKNLDLKLVLGLTLRYGTTVTATACQM
                     VHVSDKPLAVVMLNEDNTTKWCLRNSHFRDFFIKKGTLINIQEHLPEELSPTYQDTWFS
                     SSVKSVWNLKASSLDMEEYKQRIYMISGSKLT"
                     /name="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(3193..3486)
                     /locus_tag="I8N79_RS27770"
                     /old_locus_tag="I8N79_27770"
     CDS             complement(3193..3486)
                     /locus_tag="I8N79_RS27770"
                     /old_locus_tag="I8N79_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_087796370.1"
                     /translation="MLSEKIRELRTSRGLSLDQLASNVGSTKSYIWELEKKPGIKPSAE
                     LVSKLAQALDVTMDSLMDEKKENDQDIVFFREYKSLSEETKVQLMNILRAIK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3713..3895
                     /locus_tag="I8N79_RS28130"
     CDS             3713..3895
                     /locus_tag="I8N79_RS28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087796369.1"
                     /translation="MKPGNITMRSGQYEIVGPRGGHTGVEVTSVKGKPLPPTPKSGQSY
                     VLVDPTKNGSGKKSK"
                     /name="hypothetical protein"
     Typing          complement(447..928)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.152"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gctctcatca gccaataact ggatggcatt tttagttgat gggagctgcg ttttgaataa
       61 acctgcacca ccaggcttca aatgggtatt ctgccgttat cgtcgggtta aaaactcgac
      121 gagattgctt gatgcgcatg attacggcta cgaagcatgg tcatttttag tcccagggaa
      181 accgtaacca gggaggggtg aggaacctaa acacctcagt aagtaaggct gctaaggcgg
      241 ccttttttgt ttcttgacgt tgacctacag gacagcgtag tcttctttga acgaaaaaac
      301 cgccttgcag ggcggttttt tcgaaggttc tctgagctac caactctttg aaccgaggta
      361 actggcttgg aggagcacag tcaccaaaac tgttcgttta gtttagcctt aaccggcgca
      421 taacttcaag actatctcct ctaaatcagt taccagtggc tgctgccagt ggcgcttttg
      481 catgtcttac cgggttggaa tcaagacgat agttaccgga taaggcgcag cagtcggact
      541 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
      601 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
      661 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
      721 cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt tgtgatgctt gtcagggggg
      781 cggagcctat ggaaaaacgg caacgccgcg gccttctcct tgttctcttt ggctctctgt
      841 gctgtcgtct cgtgccctta tacgtgagtt gcgaccgctc gccgcagccg aacggcagga
      901 gcgtagcgac tgagtgagcg aggaagcgga aaagagaaca tccctacatt gagcactgac
      961 gctctgaggc cccttatgac gaaacaacag aaaacagcgc tgaacatggc taaattcatc
     1021 cagaatcagt ctctgctcct actggagaag cttaacgagc ttgatctcga tgtcgaggcc
     1081 gatctttgtg agaaactcca cgatgatgcg gagcatcttt tccgtacact gtcctcgaga
     1141 ctggatgcat tacaggatgg taactgatgg ccagagtgtc tattagtgag gctgcccgac
     1201 tgacgggtaa gagtagaacg acactgcaca ggttaattaa agcaggtgac ctgtccacct
     1261 gtagcggtga acgcaatgca aaaatggtag atacttcaga gcttttacgt gtctttggtc
     1321 cctttgaaca accgaagggt gaacaccatt atggacaggt ctctgaacag cgtgatacag
     1381 cctctgctga ccagagtgaa caggtgattc gtcatctgaa acaggagatt gaacacctga
     1441 acaccttact tttgtccaag gattcgcaca ttgacagcct gaaacaggcc atgctccttc
     1501 ttgaaaatca ccaggcaaaa ttaacggaat ccccggcacc atggtggaaa ttctggaaaa
     1561 aatcctgatc ctgccatgct tgagttttcc tctccggcca tcccggggag ttcctgaacc
     1621 ttcccaaaaa tcgccagatc tctgaacgct cctcacgact aaaaataccc acacactacg
     1681 caaaattaaa attttgaaga tttgtattta atatcagtag cttactttcc tgaaattccg
     1741 gtttttaaat tttatttaca tagatccgac tctatgacga ctcgtcatag agtccccggc
     1801 gagtcgtcat agagtcggtg acgactcagg gctataaaaa caatgagtta ataacgaccc
     1861 gactctagac ccgactcccc gtttggttgt tttttagact cggcgactct gggtacgact
     1921 ctagtgaaga gtcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc cataacgtga
     1981 ttgccggccg tcgacgaacc cgattcctga tcgtgctgaa aaccggtttt ccggctgcgc
     2041 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatggaaa
     2101 gaacgccgga aacaggactt atcctggctg aatggtgtca atttccgcgc tatgcacttg
     2161 cgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2221 ggtttgttac ctgttttacc ggtttcccgt cagaaacgcc ctgaggccgt tttagcggtg
     2281 cgtacaatta agggattatg gtaaatacaa tatattggtg ggtgttaaag tacggttttt
     2341 ttcatggtta ccgtagaggg tagacctgtc tgcctaagtt tattggggga ggctgtcgct
     2401 gattatgaga acaataattt taagtaagct tactgcctga aatcatatag attctttgtt
     2461 tgtattcctc catatctaaa gatgaagcct ttagattcca cacgcttttc acagagcttg
     2521 aaaaccatgt gtcttgatat gttggtgaca gttcctccgg taagtgttct tggatgttta
     2581 taagagtccc ttttttgata aagaaatcac ggaaatgcga attacgaaga caccattttg
     2641 tcgtgttatc ctcgtttaac ataacgactg caagtggttt atcagacacg tgcaccatct
     2701 gacaagctgt tgcagttact gttgttccat accgaagagt gagaccaagc acgagtttga
     2761 gatcgaggtt tttatctaag agttgctgct gtacatcttt ttctggcatc aatagacaag
     2821 ctgcaaactc attggcttct ttttctatct ccttaccatg cggtgttaga acgaatgctc
     2881 ccatgtcctc taatcggcat tgaacttcct ctctatcgct atgtagacag tagtgaccaa
     2941 tttcatgggc tatggtgaaa tttttccgcc catcttcttt tatgttttca ttgaaaataa
     3001 tggcttttac atcaggggtt ataaagaggg ccccttggaa ttcatcctta agaggggccc
     3061 cttggacttt tattcctagg ctttcagcta tcttaatgca atcaacagga agagcatcag
     3121 cgaagtgctt tcctctaagc cactgttctg aaaccagtgc cgcctggagc ctaggtgtag
     3181 tcatctagaa ccttatttta ttgctcgcaa tatattcatc agttgcactt ttgtttcttc
     3241 tgataagctt ttgtattctc taaagaaaac tatatcttgg tcgttttctt ttttctcatc
     3301 catcagagag tccattgtaa catctaatgc ttgagcaagt ttagaaacaa gctctgcaga
     3361 tggttttatc cctggttttt tttctaattc ccaaatgtag ctttttgtag aacctacgtt
     3421 tgatgctaat tgatccaagg acagcccgcg agatgtcctc agttctctga ttttttcgct
     3481 aagcatatga tattcctatt caaattgtgt ttatgggttg ggtggttgtg cttttctgca
     3541 cggttaatta caggcagtat gaaataaaag tattgctata gcaatacgac gtatcgccgt
     3601 ggcgatactt ttttgtaccg tcagggcttg actgaaccgt gagcgacaat gtattgttca
     3661 agttgaagtg atacgagtaa gttcgttttg acaatactat acagggggca ttatgaaacc
     3721 gggaaatatt acgatgcgtt cggggcaata tgagattgtc ggaccgcgtg gtgggcatac
     3781 gggtgtggaa gtgacttctg tgaaaggtaa gcctttgccg ccaactccca agtcgggtca
     3841 atcatatgtt ttggttgacc cgactaaaaa tgggagtggc aagaaaagca agtagcttat
     3901 ctttccattt tttttgtgtg gcgaagtcat ccgccagcca cataaactga tgggaaaata
     3961 aagcaaaact tagattgggg aatcgaagtg tagcttgttt
//
