LOCUS       NZ_CP065840             3478 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain KqPF26 plasmid p2, complete
            sequence.
ACCESSION   NZ_CP065840
VERSION     NZ_CP065840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016750
            Assembly: GCF_016415705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 271x, 39x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 23:32:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,244
            CDSs (total)                      :: 5,125
            Genes (coding)                    :: 5,035
            CDSs (with protein)               :: 5,035
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 44 of 90
            Pseudo Genes (incomplete)         :: 43 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KqPF26"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="p2"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-09"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            complement(180..581)
                     /locus_tag="I8N75_RS26065"
                     /old_locus_tag="I8N75_26065"
                     /db_xref="GeneID:64298051"
     CDS             complement(180..581)
                     /locus_tag="I8N75_RS26065"
                     /old_locus_tag="I8N75_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_198974790.1"
                     /db_xref="GeneID:64298051"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTGSPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(581..772)
                     /locus_tag="I8N75_RS26070"
                     /old_locus_tag="I8N75_26070"
                     /db_xref="GeneID:64298052"
     CDS             complement(581..772)
                     /locus_tag="I8N75_RS26070"
                     /old_locus_tag="I8N75_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_087497919.1"
                     /db_xref="GeneID:64298052"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELELDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1177..1310
                     /locus_tag="I8N75_RS26290"
                     /pseudo=""
                     /db_xref="GeneID:68611052"
     CDS             1177..1310
                     /locus_tag="I8N75_RS26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /db_xref="GeneID:68611052"
                     /name="replication initiation protein"
     gene            complement(1441..1779)
                     /gene="mazF"
                     /locus_tag="I8N75_RS26075"
                     /old_locus_tag="I8N75_26075"
                     /db_xref="GeneID:64298053"
     CDS             complement(1441..1779)
                     /gene="mazF"
                     /locus_tag="I8N75_RS26075"
                     /old_locus_tag="I8N75_26075"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_011977836.1"
                     /db_xref="GeneID:64298053"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(1776..2006)
                     /locus_tag="I8N75_RS26080"
                     /old_locus_tag="I8N75_26080"
                     /db_xref="GeneID:64298054"
     CDS             complement(1776..2006)
                     /locus_tag="I8N75_RS26080"
                     /old_locus_tag="I8N75_26080"
                     /inference="COORDINATES: protein motif:HMM:NF015947.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_011977835.1"
                     /db_xref="GeneID:64298054"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2198..2875
                     /locus_tag="I8N75_RS26085"
                     /old_locus_tag="I8N75_26085"
                     /db_xref="GeneID:64298055"
     CDS             2198..2875
                     /locus_tag="I8N75_RS26085"
                     /old_locus_tag="I8N75_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977834.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_225372707.1"
                     /db_xref="GeneID:64298055"
                     /translation="MIYKEKKINRNQIPVILASRWGYSILEEAGWTTVPNLLLEKQMVL
                     ELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTVQRTINKLISKGFLKRKI
                     RAKSVNDKGLSNLYSFEPLIEKLLISISNNKGQYEEPQKEQGERDLKTVDQLVRIAAAG
                     GGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKTVDELVRIAAAGKGCVLF
                     ELE"
                     /name="helix-turn-helix domain-containing protein"
     Typing          821..1278
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.712"
                     /coverage="90.0"
                     /evalue="3.64e-149"
ORIGIN
        1 atacagcgta atttaataac acccaactaa ctgatattaa ggagttatct tcaaaatttt
       61 aattttgcgt ggggtgtggg tatttttagt ggtgatgagc gttcagagat ctgacgattt
      121 ttgggaaggt tcaggaactc cccggaatgg ccggagagga aaactgaagc atgacggtgt
      181 caggattttt tccagaattt ccaccatggc gccggggatc cagttaattt tgcctggtga
      241 ttttcaagaa ggagcatggc ctgttttagg ctgtcaatgt gcgaatcctt ggacaaaagt
      301 aaggtgttca ggtgttcaat ttcctgtttc agatgacgaa tcacctgttc attctggtca
      361 gcaaaggctg tatcacgctg ttcagagacc tgtccagaaa gatgttcacc cttaggttgc
      421 tcaaaggaac caaagacacg caaaagctct gacgtatcta ccatttttgc attacgttca
      481 ccgctacagg tggacaggtc acctgcttta attaacctgt gcagtgttgt tctactctta
      541 cccgtcaatc gggctgcctc actaatagac actctggcca tcagtaacca tcctgtaatg
      601 catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcatgg agtttctcac
      661 aaaggtcggc ctcagcatca agttcaagct cgttaagctt ctccagcagg agcagagact
      721 gatgctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataagaagcc
      781 tcagagcgtc agtgctcagt gtaaaaccag tctcttttcc gcttcctcgc tcactcagtc
      841 gctacgctcc tgccgttcgg ctgcggcgag cggtcgcaac gcacacataa cggcatagga
      901 cagacagcac ggagagggac aaggccgcac aacggccgtt tttccatagg ctccgccccc
      961 ctgacaagca tcacagaatt tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     1021 aaagatacca ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cccgcctttc
     1081 ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc tcattccacg cctgacactc
     1141 agttccggtt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     1201 gactactgcg ctaattcggt aactatcaac ttgagtccaa cccgaacaag cacgccaaag
     1261 cgccattggc agtagccatc ggtcgctcta cgggggaaag aaaatcttga ttgatgtaac
     1321 agtaagcagt tacacttgat aaacaatatt ggttactgtt cccctttaga gcagtcaccg
     1381 aaggccgaaa atgctggtaa cattctcggc ctgaaaagac cggcttatgc cggtcttttt
     1441 ttatgcttta ataatttttg aaaccatatc aacaactgta gttactgtct cgccggaaac
     1501 gctatcaaca gtacgtgccg ctctggcctt ccagtctaaa gacttcactt gatcagccaa
     1561 gataacccct gttgttttag tagtcccatc aacaggaact tcaaaagggt acccttttat
     1621 ctgattagtt atggggcaaa caacagcaaa cccagtgact tggttaaaca aatcatcaga
     1681 aacgacaaga gcaggccttt tccctgcctg ctcatgccct gattgagggt taaaatctaa
     1741 aagaatgaca tcgcctctgg ctggacaata tgtcgtcaca gcatctcctt accttgagag
     1801 tcacatagat actcgtgatg gcgattttct gcagtgcact gtccgagcag ttcttcaagc
     1861 gtcgggccag ctttagtaat tactaaatcc ctttcagtga cgcggaaatt aacaacatca
     1921 ccgatactaa gcccaagctg cttcatgaac ggctgcggga tacgcaaagc agcgccatta
     1981 ccccatttac caatagccgt agtcataata cctccaaagc agatggataa acaatgtgta
     2041 aacaatattt gtattgtata gtctttgagt tcagtttgaa gggtggcatc aatgccaccc
     2101 taaaaagggg taactactta aacaaataaa tatttatttg tttaagtagt taccaactgc
     2161 aaaaaaaaat tttttttaac taagtggtga taacaaattg atttacaaag aaaaaaaaat
     2221 aaaccgcaac caaataccag tgatcttagc aagcaggtgg ggatatagca tcctagagga
     2281 agcagggtgg acaactgttc ctaatttatt gcttgaaaaa cagatggttc tagaattgag
     2341 taacaccgaa atgctgttac tcattcatct aatctctttc attcactttg cagacacccc
     2401 agcattccca agcatttcta cactagcagg taggatcaac caacacccaa gaaccgtgca
     2461 gcgaaccata aacaaattaa tatctaaagg gttcttgaag agaaaaatcc gcgcaaaatc
     2521 tgtcaatgac aaaggcttga gcaatcttta cagctttgaa cctttgatag aaaagctatt
     2581 aatatcaatt agtaacaaca aaggccagta tgaagaacct cagaaagaac aaggagaaag
     2641 agacttgaaa acagtagatc aattagttcg gattgctgca gcaggaggtg gtttacgttt
     2701 aagtgcatcc acaaaaacag tggatcagct cgtgcggatt gccgcagccg caggtaactc
     2761 gataaccccc aataaagcaa agttagttct gtttgatctt gagtccaaga ctgttgatga
     2821 gctagttcga attgctgcag caggaaaggg gtgtgtacta ttcgaattgg agtaacacac
     2881 taaaaagatc actataagtt taaacatcaa accagtctta atttaacata aagccctatt
     2941 acgtgcaccg ctaaaacggc ttcagggcgt ttctgacggg aaaccggtaa aacaggcaac
     3001 aaaccacccg aaaacctgcc agaaacgcgc aggggctgtt tttacggcat acatgagtat
     3061 gcatgcaagt gcatagagcg ggaattcatg ccattcagcc agggtaagtc ctgtttccgg
     3121 cgtttttctg atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaggcgt
     3181 tgccggcgca gccggaaaac ccgttttcag cacgttcaag aatccagttc agcgacagcc
     3241 ggcaatgacg tgatggcaag tcaccggacg acttagcaca cgattcaccg gcgattcgac
     3301 tcgtgacttg actctagagt caccaaggtc aaaaaaacac caaatgaacg actcaactct
     3361 agagtcaagt cgtcataagt tactgatagt atggagtgac ttgtcgatga ctcgtcatag
     3421 agtcatcgac aagtcgtacg cgaatcgccg acgacttgga ttgaccttac agaaggta
//
