LOCUS       NZ_CP065837             3550 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KpC5 plasmid p4, complete sequence.
ACCESSION   NZ_CP065837
VERSION     NZ_CP065837.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016747
            Assembly: GCF_016066475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 284x, 150x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 23:01:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 80 of 140
            Pseudo Genes (internal stop)      :: 32 of 140
            Pseudo Genes (multiple problems)  :: 25 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065837.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpC5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-05"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            join(3422..3550,1..1404)
                     /locus_tag="I8N78_RS26495"
                     /old_locus_tag="I8N78_26495"
     CDS             join(3422..3550,1..1404)
                     /locus_tag="I8N78_RS26495"
                     /old_locus_tag="I8N78_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_134366960.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPIINSYYKINNLEKKNIHHYLWVTGNVYNKDLKRLDSFKN
                     NNKNIDVDYLFGEHLDDFFYKKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     gene            2309..2458
                     /locus_tag="I8N78_RS26500"
                     /old_locus_tag="I8N78_26500"
     CDS             2309..2458
                     /locus_tag="I8N78_RS26500"
                     /old_locus_tag="I8N78_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138881.1"
                     /translation="MPSEYDEQRDATDTQLPGEYDLFDLMAKVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          2101..2316
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.845"
                     /coverage="100.0"
                     /evalue="7.43e-58"
     Typing          complement(1453..1932)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.137"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 agcacggaca taggtaagct gccatttttg aaattcagcc ataacaggag attcatttat
       61 ctaaaagatg attttggcag tttcaaattt tggcgagctc tcgaaaagtg catgtacgaa
      121 gcaaactcaa cttactcaca cgcgatactt gccgtaataa ataatggcgg ttatttgaag
      181 gtgaaagatt ttgggattgt gtctggatct cctataaaac aagctaaaca tttaagttat
      241 gaaacagttc taagaaatct actttcggca aaaatactta gagccgtgta tattgatgga
      301 gttggtgatt gcgtactgat aaataacaac atagccaacg atgttaatat tagaacaatg
      361 gcaaattgcg aatccttttt tgacaaacca atattggagc ttgttaaagc ttggttgagg
      421 aatttaggcc tagtcgcttt taatcagata aaaacaaaat atgatgggga gggaaatcca
      481 gttgtaggct cttttgaatg ggatatgacc gctccaagtt acgtttcccc tctcgctgaa
      541 tatgtcggcg gaaaactgat gcctggtttc gttgcctgtg atttttctct cggctttaat
      601 cgtgatgaaa ttactaccgc tgcagcagaa acattcataa gaaaagtcca gatgaccaag
      661 tcttctcgag cgagtcagag gataatgttt gttattttcg caaggcgttt cggcaaaata
      721 gccttcaata agttgagaag tgagggggtt ctggctgtaa ctatagcaaa tgcgtttggt
      781 aataaagttg atgaaagctt aaccaggttg tcgagggtgg ttcagggttc tttgtccatt
      841 gaaaagcatc cagatgagtt gcttcagatg gttaaagatc tggagtctgt ttctggagag
      901 aatggcaatt tgagagggta tatttttgag ctttttgtat cctcgcaaat ttctaatttt
      961 tatggccttg gtaatattta cattaataga gagtataaga taaatggtaa gcatgcagaa
     1021 gctgatgttg tattggagag tagtgacgat atttatataa tagagtgcaa aaatgttaag
     1081 gtcttacctt caactgaact tacgcgttgg atgaaagaaa gggttccaat tataaactct
     1141 tactataaaa taaataattt agaaaaaaag aatatccacc attatttgtg ggttacaggg
     1201 aatgtgtaca acaaagattt aaaaaggtta gactctttta agaataataa taagaatatc
     1261 gatgttgatt atttgtttgg tgagcatttg gatgatttct tttacaagaa aaagcgggtg
     1321 ttcaacttat atagaaaagt catttcacct gattataaaa aaggcgtaat gcctgatttt
     1381 gagttagagg acgatttttt ctagctactg cgtttcttga cgatggcgct ttgaaaggcg
     1441 tatcttccat ctctgaaacg aaaaaaccac ccggcaaggt ggtttttcga aggttcagga
     1501 aaccggcaag tttctgaacc gtggtaactg gcttaaacgg agcgcagtga ccaaatctgt
     1561 ccttccagtg tagccgtagt caggtcaaca cttcaagaac tctgtgtatg cttgtacacg
     1621 cctctccgct ccgtaaacca gataccaatg gctgctgcca gtggcgtttg tcgtgtcttt
     1681 ccgggttgga ctcaagtcga tagttaccgg aacaggcgca gcagtcggac tgaacggggg
     1741 gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg
     1801 gaatgagata aacgcggcca taacagcgga gtgacaccgg taaaccgaaa ggcaggaaca
     1861 ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag tcctgtcggg
     1921 tttcgccacc actgatttga gtgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     1981 tggaaaaacg gcagcgccgc gtccttttcc tgccctgttt gtcatgccac atgcttttgc
     2041 tcttatccgt gtgcagctaa cgcccgccgc agccgaacgg caggagcggc agcgaccgag
     2101 tgagcgagga ggcgtcatat cacagtaacc cccattttct cccgtacgcc ctgtagctgc
     2161 attttcccct gtcacatgaa gcactttcct gaaccccgac cccgccgtaa catattcagc
     2221 tagtttagac tccgctagcg ctacgtgact ggttcagggc tgcgctccga aacccgctaa
     2281 caacccctta gctactggat atcaccccat gccatctgaa tatgatgaac aacgggatgc
     2341 gactgatacg cagcttcccg gcgaatatga cctttttgat ttaatggcga aggttactga
     2401 agagaacctg cacgccccgg tggattttgg gccgcctgtc ggccgggaag cgttatagct
     2461 ttttcgctta cccgcgtacg tgtaatacgc cggtattaca tcttatttct ttattttcat
     2521 gtgattacag gtggttgtaa tacgttgcgt attacataaa taaacgggaa cttcagtttt
     2581 taccttttaa ttcatgcaat tgcgatgaaa atcgcggttg cgtatggtgt atggccattt
     2641 aagggataaa gaatcatctc cttaccgggc tgagctttgc ctattctgag gagatctgtg
     2701 aaaaaaagcg tccataacta gctcgttcat acttgtatga acagcttatt cctcctgact
     2761 actaataaac tacagcgtca ctactgattt actactgatt tactacaaat cggaggagga
     2821 tgactactta tctactactg atttactact gaattactac tgaattacgt cataacagtt
     2881 ctctgaacca ttcgtacatc cgaccccctt ctcttcatgc ttgatgctct tgccaacgct
     2941 tcacggtcac gcaataaatt tcgtccccgc tcaccgtttt cggatgagaa gaacgccgga
     3001 aacaggactt ccactggctg cacggcgtga aattctgcgc tatgcacttg cgcgcatact
     3061 catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc
     3121 ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt gcatgcaacg
     3181 gatgttatgg taaatggagt agctgatagt tcgataaaaa aatgtcacaa tactatcttt
     3241 atgatttaaa aggattaaat aattataaag acgatatttc tttgtgctca atttaaagta
     3301 agtaattaac catctgattt ttaattattt tttatggtca cttttttttt aaaagactgc
     3361 gccctattac acgtagtgta atattataaa tagcgtagtg tgacagaaat aaggtgagga
     3421 tatgaaaaaa agtgttgtta gacaggtgct tgaaatgagc gggccatgca tctcttcgga
     3481 tcttgcagag agaattcagt ggcaacatcc ctcaatgtcc cctgaagcga tacgtaaaat
     3541 gatctcgcga
//
