LOCUS       NZ_CP065835             5542 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KpC5 plasmid p2, complete sequence.
ACCESSION   NZ_CP065835
VERSION     NZ_CP065835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016747
            Assembly: GCF_016066475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 284x, 150x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 23:01:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 5,072
            CDSs (with protein)               :: 5,072
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 80 of 140
            Pseudo Genes (internal stop)      :: 32 of 140
            Pseudo Genes (multiple problems)  :: 25 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpC5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-05"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            complement(join(5485..5542,1..674))
                     /gene="mobC"
                     /locus_tag="I8N78_RS26435"
                     /old_locus_tag="I8N78_26435"
     CDS             complement(join(5485..5542,1..674))
                     /gene="mobC"
                     /locus_tag="I8N78_RS26435"
                     /old_locus_tag="I8N78_26435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_064190304.1"
                     /translation="MLISDPTERRLASESKRLKILKFLRDEIWSSANVLAAVAGLQSAQ
                     AIHKTLTQLERDGMVRRHLFPIAGRVTKTIWGITPHGLAHAWSDDEQYEDRPRFEPSKL
                     VLARVPHQLDMQEARLKAEASGWGEWVRGERLGFKPAIRPDAMTVSPKGLKVAVELERT
                     IKTKLRYQVIIREHLRAIRTGQWQVVVYITPDGMPLRLKRIFDSIDYVSINGQHEILSD
                     KHRNLFRFISLSDWPSMEKSI"
                     /name="mobC"
     gene            complement(661..2502)
                     /locus_tag="I8N78_RS26440"
                     /old_locus_tag="I8N78_26440"
     CDS             complement(661..2502)
                     /locus_tag="I8N78_RS26440"
                     /old_locus_tag="I8N78_26440"
                     /inference="COORDINATES: protein motif:HMM:NF014581.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_198068767.1"
                     /translation="METFNYLINIVFSSLVTIYFFLVPFGGFLSVFLGLIYLTANPEKR
                     QKLQSLGLLSLVIGLINPLGYFWIPLLRSYVYSVDYELLFPLGFLALTLGSAFLGFAAW
                     LGWTRYCVPFINKYLHQFTRRTELERNRKTDVREIEKFLPTAKLDYNPDKWLSVDPKKT
                     GLFAGINELGKPIFIPYEKWRSAHVQIVGTSGFGKGVATGILLSQAMRAGETVIVFDPK
                     NDEWAPHVLRQSAERAEVPFYLVDLESDFGQLDLLAGATAFEIEELLSAGFSLGDSGDI
                     ADHYRLKDRAAIRNISQLVNAGARTVADLAYSELANSYEKECEGAIFKLRELALCRAIS
                     GVGGIDMKTVVKNGGCLYIIGSMDNSRLMMAQKMLLVRLQQIVKKRDRITETPRPVCAF
                     LDEVKYLLSRKSLEGLGAARDKGLHFVLTHQSLGDLKDCPRDLNPEAVVGAIVENCSIR
                     IAYRVQNPDTALWLAKMTGEIQVDDETRRVERNAGGAELLSHERTIRQSTRNLIDTNML
                     LNLPGRTAVVFGSGQPQFAHICPILAEKKPLTVYVAPEPVVPVVEPLIPAPAVTTVPVE
                     KNSKPKPQPKKTVPIETRNYEDDDMHDYLQEYNNADF"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            2633..2827
                     /locus_tag="I8N78_RS26445"
                     /old_locus_tag="I8N78_26445"
     CDS             2633..2827
                     /locus_tag="I8N78_RS26445"
                     /old_locus_tag="I8N78_26445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198068768.1"
                     /translation="MVQEPGGSKHNRRSTVPRTVAGCMGGPTTKNGPSHFSAAHSAVVS
                     WGRSPPSEERERRLTPGIN"
                     /name="hypothetical protein"
     gene            3179..3895
                     /locus_tag="I8N78_RS26755"
     CDS             3179..3895
                     /locus_tag="I8N78_RS26755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_227657057.1"
                     /translation="MTNLVGRQNGRGGTARRSRTTERSELVSEEAEFHLSGHKNQYALM
                     PKNGRVRVRTYIRYRRGYPGSRANAGFFLTIVISQDNLVISQDNYQGRFMKRVGQIDLD
                     TGEEIGSFVAVIRPKQKSTFQRHFTMNQAALLTIANELNHEQMRVLMALLAELDYENYI
                     QVAQTDISVALQMQKTNVSRAIKNLLEFGIILEGPRIGRSKTYRLNPQFGWKGTVSNHK
                     KALKNGLSVIQGGRAV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3965..4246)
                     /locus_tag="I8N78_RS26455"
                     /old_locus_tag="I8N78_26455"
     CDS             complement(3965..4246)
                     /locus_tag="I8N78_RS26455"
                     /old_locus_tag="I8N78_26455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198068763.1"
                     /translation="MKKVIFSLIVTLGLTACAGKPTGDITLLSDPNGGNEVKTYRMGYT
                     CYVHYNPQNYRSADVLRGVDTCGEMVGATNSEKFLKYRADVESKLSNS"
                     /name="hypothetical protein"
     gene            complement(4257..5153)
                     /locus_tag="I8N78_RS26460"
                     /old_locus_tag="I8N78_26460"
     CDS             complement(4257..5153)
                     /locus_tag="I8N78_RS26460"
                     /old_locus_tag="I8N78_26460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198068765.1"
                     /translation="MKNKFLYVALAASITAGCDQSSPAGASVSSGESSTQEQTQQVQRN
                     KQISTGQGANNITETAAFPLIQRAIADIYPNLPYDVGVNFCGPAREGKKIEEVTESLKS
                     EIGATEYSEYLKLVDGVTNINLACAVSLVRFSVNPINGWVIGQTYTDQAKVLLAVSIAS
                     SKVLSPIAVEISKKPGMNEKELTSLIQNRFMDDNEAVFYEMRNELQKNRNYVIDATGVK
                     GIHFTTSDGYDVIGNGNGVTVSNYGTTWYGSGYIEGNFYKVSTISSRAINMTKDNTITS
                     GSSISSGESSSADISTK"
                     /name="hypothetical protein"
     gene            complement(5156..5485)
                     /locus_tag="I8N78_RS26465"
                     /old_locus_tag="I8N78_26465"
     CDS             complement(5156..5485)
                     /locus_tag="I8N78_RS26465"
                     /old_locus_tag="I8N78_26465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126478625.1"
                     /translation="MPSPLAIMAVIAPSALLGAFLFFFVYDEHSTKNDIQRDKHQLETM
                     KFDRDFSNAWNGDSVTPPKENDIASMEEKIKMKEEKAKKEKEERERRIKKIQDDVEHTI
                     AKSGE"
                     /name="hypothetical protein"
ORIGIN
        1 cggtgtttgt cagataagat ctcatgctga ccattaatag aaacataatc aatagagtca
       61 aaaattcgtt ttagacgcag gggcattcca tcaggagtaa tgtatacaac aacctgccac
      121 tgacctgtcc tgatagccct gaggtgctcc ctaataatga cctgatagcg cagcttagtt
      181 ttgattgtcc tctccagctc taccgcgacc tttagccctt ttgggcttac agtcattgcg
      241 tcagggcgaa tagccggttt aaacccaagg cgctcccctc tcacccactc accccagccg
      301 ctagcctcag cctttaacct ggcctcttgc atgtctaact ggtgcggaac ccgcgccaga
      361 accagttttg atggttcgaa tcgtggtcgg tcttcgtact gctcatcatc actccacgca
      421 tgggctagcc cgtgcggagt gattccccag attgttttag ttacacggcc agctatcggg
      481 aagaggtgcc gtctgaccat tccgtccctc tccagctgcg tgagcgtttt atggattgcc
      541 tgagcactct gtagtccagc tacagcagcg aggacgttgg cagaactcca gatttcatcc
      601 ctgagaaatt taagaatttt cagtcgctta ctttcagacg caaggcggcg ctctgtcgga
      661 tcagaaatca gcattgttat attcctgtag atagtcgtgc atatcatcgt cctcatagtt
      721 ccgagtttct atcggcactg tttttttagg ttgaggttta ggtttcgaat tcttttccac
      781 cggtacagtg gttacagctg gggcagggat tagtggctcg actaccggca caaccggttc
      841 aggtgctacg taaactgtca gaggtttttt ttcggccaga atcgggcaaa tatgcgcgaa
      901 ttggggttga ccagagccaa aaaccacagc ggttctgcca ggtagattta acagcatgtt
      961 tgtatcgatc aggttccggg ttgactgcct gatagtgcgt tcgtggctga gcagctcagc
     1021 tccgcccgcg ttccgctcaa cccgccgcgt ttcatcatca acctgaattt caccagtcat
     1081 tttagccagc cagagcgccg tatcagggtt ttgaactctg taggcaatac gaatcgagca
     1141 gttttcaaca atggccccta caacagcctc agggttcaaa tcccgggggc agtctttcag
     1201 gtcgccgagt gattgatgag taaggacaaa atgcagcccc ttatcccggg cagcgcctag
     1261 gccctccaga gattttctgg acagaaggta cttaacctca tccagaaaag cacagacagg
     1321 ccgcggtgtt tctgtaatcc ggtcgcgttt tttaacgatc tgttgcaggc gcaccaacag
     1381 cattttttgg gccatcatca gtcgggaatt gtccatcgag ccgatgatgt acaggcaccc
     1441 tccgttttta acaaccgtct tcatgtcaat accaccaaca ccagaaatgg cgcggcatag
     1501 agccagttca cgcagtttga aaatcgcgcc ctcgcattct ttttcgtatg agtttgctag
     1561 ctcgctgtag gccaaatcag ccacagttcg cgcgccggcg ttaaccagct gcgaaatgtt
     1621 ccggatagca gcgcgatctt tcagcctgta gtggtcggca atatccccgg aatccccgag
     1681 ggagaacccg gcagaaagca gttcttcaat ttcaaatgca gtagccccgg ccagcaggtc
     1741 cagttggccg aaatcggact ccagatcaac caggtagaac ggcacttcag cccgttcggc
     1801 ggactgcctc aaaacgtggg gtgcccattc gtcgttttta gggtcaaaaa caataacagt
     1861 ttcccctgcc cgcatagcct gcgacagcaa aatcccagtt gccacccctt taccaaaccc
     1921 ggacgtacca acaatctgga cgtgggcgga acgccatttc tcgtagggga tgaaaattgg
     1981 tttacccagc tcattaatcc cggcaaacag gcctgttttt ttaggatcta cagagagcca
     2041 tttatccggg ttgtagtcca attttgcagt aggtagaaat ttttcaatct cgcgaacgtc
     2101 ggttttgcgg ttacgctcca gttcagtccg ccgggtgaac tggtgcaaat atttgttgat
     2161 gaacgggaca caatacctag tccatcccag ccatgctgcg aaccccagaa atgcagatcc
     2221 cagagttaac gccagaaacc caaggggaaa cagcagctca taatcaacgc tgtagacata
     2281 agatcgcagc aacggtatcc agaaatagcc tagcgggtta attaatccga ttaccagaga
     2341 cagcagaccc agagactgca atttttgccg tttttccggg ttggcagtta ggtaaattag
     2401 ccccaaaaaa acgctcaaaa aaccaccaaa tgggaccaaa aaaaagtaaa tagtgaccaa
     2461 tgaagaaaaa actatattta tcagatagtt aaatgtttcc attgtttaaa tcatccagag
     2521 gcatcaacga gaaaaaagga tgccctggtg ctgatcatct gtccagttaa aattcgtaca
     2581 ggaccagaaa gcgggggagc gtagcgaggg gcggcagccc ccactggtgc ctatggtgca
     2641 agagccaggc ggctcgaagc acaaccggcg ttccaccgtc ccccggacgg tagccggttg
     2701 catggggggg ccgaccacca aaaatggccc cagccatttt agcgctgccc acagcgcggt
     2761 ggtgagttgg gggcgtagcc cgccgtcaga ggagcgcgag cgacgcttga ccccggggat
     2821 taactagact cgttcagagg ctaggtgaat ttccggggtc aagcgtagcg agacggcccc
     2881 gggtgaatgg tgctgattgt gagggcctcc ccgaacaatc agtgccagag ggcgaagccc
     2941 tccagatgtg agcggcgtcc agccgatcgc gtctggttgt cttttctggt tgatagcgaa
     3001 tgtccaagag catcaaccag aggagttgcc agcaactccg gcagttaaaa gcgctgctca
     3061 ggcaggtgga tggctcactg cgtgaaccct ccacccctgt aaagcgtgct tttcggtgca
     3121 gtcgtcaacg actaaatcgg aacccaccgc aaagcggcgg ggccccacga tttagccatt
     3181 gacgaattta gttgggagac aaaacggcag aggaggaacc gctcgccgca gccgaacgac
     3241 cgagcgtagc gagttagtga gcgaggaagc ggaatttcac ctgtccgggc ataagaatca
     3301 atacgctctg atgcctaaaa atggccgtgt gcgtgtgcgc acgtatataa gatacagaag
     3361 gggctatccg ggaagccgcg ccaatgctgg attttttttg acaatagtta tctctcaaga
     3421 taacttagtt atctctcaag ataactatca gggtagattc atgaaaaggg tgggacagat
     3481 cgatctcgat acaggtgagg agatcgggtc attcgttgca gtaatcagac ctaagcaaaa
     3541 atcgacattt cagaggcatt ttacgatgaa tcaagccgcc ctcctgacca ttgcaaacga
     3601 gctgaaccat gagcaaatgc gcgttttaat ggcactgtta gccgagctcg attacgaaaa
     3661 ctacatacag gtagcccaaa cagatatctc agtagcgctc cagatgcaga agaccaacgt
     3721 tagccgagcg attaaaaacc ttctggagtt tggcatcatc cttgaggggc ctaggatcgg
     3781 acgttcaaaa acatatcggc taaaccctca gttcggttgg aaagggactg tgagtaatca
     3841 caaaaaggcc ctcaaaaacg gcctgagcgt cattcagggc ggccgggcgg tataatccat
     3901 ggtaggaagt caccagaggg ccttaaaacg attctagggc cctctgaggc gtttttagcc
     3961 gctatcagga gttgctcagt ttagattcca catcggccct gtatttcagg aatttttccg
     4021 agttagtggc accaaccatc tccccgcagg tgtccacacc gcgcagaacg tccgctgaac
     4081 ggtagttctg tgggttatag tgaacgtaac aggtgtaccc catgcggtag gttttaacct
     4141 cattcccgcc gttagggtca gaaagtaacg taatatcccc ggtaggtttt ccggcgcagg
     4201 cagttaaacc cagagttaca atcagagaaa atataacttt tttcatttcc taatccttat
     4261 ttagtgctta tatctgcgga tgagctttct ccgctggaaa tagaacttcc agaggtgatt
     4321 gtgttgtctt ttgtcatgtt tattgccctg ctactgatag tggaaacttt atagaagttc
     4381 ccttctatat atccgctccc ataccaggtg gtgccatagt tagaaactgt cacaccattg
     4441 ccgttaccaa tcacatcata tccatcagaa gttgtgaaat gaatcccctt tacaccagtt
     4501 gcatctatta cataatttct gtttttctgt aactcattac gcatttcata aaaaacagct
     4561 tcattatcat ccataaacct gttttgaatt aatgacgtta attctttttc attcatgccc
     4621 ggtttttttg aaatttctac agctatcgga gatagtacct tggaggatgc gatagagact
     4681 gcaagtaaaa cctttgcttg gtcagtatat gtctggccga tgacccatcc atttataggg
     4741 ttaacagaaa atctaaccaa agaaactgca caggcaagat ttatattggt tacaccgtcg
     4801 actagtttca aatattcaga gtattcagta gcgccaatct cactctttaa tgattcggta
     4861 acctcttcaa ttttctttcc ttcccgcgca ggaccacaaa aattaacccc aacatcataa
     4921 gggaggttgg ggtagatatc agcaattgcc cgttgaatca aggggaaagc agcagtttcg
     4981 gtaatattgt tagcgccctg ccccgtagat atctgtttgt ttcgctgaac ctgttgcgtt
     5041 tgttcctgcg tgctgctctc ccccgagctg acgctagctc cagctggaga actctgatcg
     5101 cagccagcag taatagacgc agcaagtgct acatacaaaa atttattttt catctttact
     5161 ccccagattt tgctattgtg tgctcaacgt cgtcctgaat ttttttaatt ctacgttctc
     5221 tttcttcttt ttcttttttt gctttctcct ccttcatttt tattttttct tccatgctgg
     5281 cgatgtcatt ttcttttggt ggtgtgacgc tgtcaccatt ccatgcatta gagaaatcgc
     5341 ggtcgaattt catggtttcg agttggtgct tgtctcgctg tatatcgttc tttgtactgt
     5401 gttcgtcata gacgaagaaa aacaaaaacg cccccagaag cgcggagggt gcaattacag
     5461 ccatgattgc taatggactc ggcattaaat agatttctcc atgctgggcc agtctgaaag
     5521 gctaatgaat ctgaatagat tc
//
