LOCUS       NZ_CP065830             3303 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KpPF25 plasmid p5, complete sequence.
ACCESSION   NZ_CP065830
VERSION     NZ_CP065830.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016749
            Assembly: GCF_016066025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 311x, 154x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:52:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 5,122
            CDSs (with protein)               :: 5,122
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 55 of 127
            Pseudo Genes (incomplete)         :: 65 of 127
            Pseudo Genes (internal stop)      :: 22 of 127
            Pseudo Genes (multiple problems)  :: 12 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065830.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpPF25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-09"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            complement(211..633)
                     /locus_tag="I8N74_RS26720"
                     /old_locus_tag="I8N74_26720"
     CDS             complement(211..633)
                     /locus_tag="I8N74_RS26720"
                     /old_locus_tag="I8N74_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004216558.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(633..824)
                     /locus_tag="I8N74_RS26725"
                     /old_locus_tag="I8N74_26725"
     CDS             complement(633..824)
                     /locus_tag="I8N74_RS26725"
                     /old_locus_tag="I8N74_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1292..1441)
                     /locus_tag="I8N74_RS26730"
                     /old_locus_tag="I8N74_26730"
     CDS             complement(1292..1441)
                     /locus_tag="I8N74_RS26730"
                     /old_locus_tag="I8N74_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165383661.1"
                     /translation="MPSEYDDPRDSTDTQLPGEYDLFDLMAKVTEENLHAPVDSGPPVG
                     QEAL"
                     /name="hypothetical protein"
     gene            2197..2718
                     /locus_tag="I8N74_RS26735"
                     /old_locus_tag="I8N74_26735"
     CDS             2197..2718
                     /locus_tag="I8N74_RS26735"
                     /old_locus_tag="I8N74_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023325449.1"
                     /translation="MSDLQNKLVFSESPRVELGSNFFINVPVILQYKDTPLISVESAVD
                     ASFTTSIPIYHNDGTYLAKAKGTQLYLTEEGRKAGVTLRHEDKITVCLINGKEVFELRR
                     QSAAALKAEAELYVPDGKLISYRGSSPKLFSTENNQLAIGGFVMSGSTFSGCKIGILLD
                     ENSIAIGVNR"
                     /name="hypothetical protein"
ORIGIN
        1 accgacgact tggattgacc ttacagaagg taatactgcg taatttaata acaaccaact
       61 aactgatatt aatgagttat ctgcaaaaat ttaattttgc gtggggtgtg gatattttta
      121 gagtgaggag cgttcagaga tctggcgatt tttgggaagg ttcaggaacg ccccgggatg
      181 gccggagagg aaaactcaag catggcggga tcaggatttt ttccagaatt tccaccacgg
      241 ggaatggggt ggtgaagaag gttcgtgctt gtgctccaga agctgcatag cctgacgaag
      301 ggaatcaata tgctcatctc gagccttaac agccccttta agaccatcgt tttcagcttc
      361 aagtaaagcg attctatgtt gtaaatcatg taaaccaacg ctgttaatac ctgtagattc
      421 tgcgtcactt tttacatcat attttacagc atcgattaca ccatcaagag aaccaaacac
      481 tctaataagc tctgatgtat caataacttt aatacccttc ccgtgcttta caacagaggt
      541 gtaacttaac ttaccactgt taatcatctt gtaaatagta gggcgactaa ccttaacaag
      601 ccgtgctgct tcactaatag acactctggc catcagttac catcctgtaa tgcatccagt
      661 ctcgaggaca gtgtacggaa aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg
      721 gcctcagcat cgagatcaag ctcgttaagc ttctccagca ggagcaaaga ctgattctgg
      781 atgaatttag ccatgttcag cgctgttttc tgttgtttcg tcataaggga cctcagagca
      841 tcagtgctca gtgtaaaacc ggtaaggggt cggatgtacg aatggttcag agaacggatc
      901 tgaggtaatc ctgtagttat tttgtagttt atatgtagtc atcgcaaaaa atcaggatta
      961 agtcatcagc ttttgtagtc ttcctgtagt ccggaaaaat aatccgttca tgcaagcatg
     1021 aacgaagcag ttaaagacgc tttttcgtca ccgatgcacc ttaacctgcg cagttaaccc
     1081 agccagaatg ataatttttt gccccttaaa tgatcataca cctaacgcag ccgcgatttt
     1141 catcgcaact gcatgaatta aatggtaaaa atggaaatat tcgtttattt gtgtaatacg
     1201 caaggtatta caatcacatg taactacatg aaaataaata aataagatgt aatacctgcg
     1261 tattaccacg tacgcgggca agcgaaaaaa gttataacgc ttcctggccg acaggcggcc
     1321 cagaatccac aggggcatga aggttctctt cagtaacctt cgccattaaa tcaaaaaggt
     1381 catattcgcc gggaagctgc gtatcagtcg aatcccgtgg atcatcatat tcagatggca
     1441 tgtgataata tccagtagct gagaggttgt tagcggtttt aggggtgcaa ccctgaaccc
     1501 gtcacgtagc actagcggag tgtatactag cttgacatgt tgccatgagc gaaaagtcag
     1561 gtgaagtgcg ccaatatgca tgaccagagt gtccggtgaa ccatgccaag gcagtggctc
     1621 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtgag
     1681 ggtgtctttg tggggatgtt tttgattctc tggctttaaa tgtacaaaag ccccgtgaca
     1741 gaatcacagg gcttcggtac tctaattctt cgcagatgcc tgccaggaat agacgtcaga
     1801 aatcaaatcc tagcagggct ggctagcatt gtcactacca gcttaatacc attcaacgac
     1861 attaaaataa tatctgcacc ttagacactc aggtctacgc cccggactcc ggcaggataa
     1921 acaggcaatt ggtggatatg taacttaacc gcaaaagagt taagctctac cccgtctcgc
     1981 tgtacgataa caagcacctg ataaaacgag ggtctaagaa agtagtaagc ggagtagtgt
     2041 gattgaaagc gtcggacact acacccacac aatcgaacct tttgcagggt gagacacaca
     2101 agaaacgagc tggacgaagg tcgctacgct ccccgtgatt tttatatacc caccacgggg
     2161 gcattaggtt ttttttgaca aaaaaaggat gtatccatga gtgatttgca gaataaactc
     2221 gtatttagtg aaagccctcg agttgagctt ggttctaatt ttttcatcaa cgtcccagta
     2281 atacttcagt ataaagacac cccacttatt agtgttgaga gtgcggttga tgctagtttc
     2341 actaccagca tccctatcta tcacaacgat ggaacatacc tggccaaggc taaaggaacg
     2401 cagctctacc ttactgaaga aggtagaaag gcaggggtga ctctccggca tgaagataag
     2461 ataactgttt gtcttattaa cgggaaagag gtattcgagc tcagaagaca aagcgcagct
     2521 gcactaaaag cagaggctga gctttatgtt cctgatggaa agctaataag ttatcgagga
     2581 tcctcgccaa agttgttcag cacggaaaac aatcaactag ctataggcgg ttttgtgatg
     2641 tccggatcaa cattcagcgg gtgtaaaata gggatattgc tagatgaaaa cagcatagcg
     2701 attggtgtaa atcggtagcc gcattggtta tttcttgcgc gcgcaataaa agatgatggt
     2761 ttatcccgct cacttgtatt taccataaca tccgttgcgt gcaccgcctc agaggccctc
     2821 agcgcgtttc tgaggcctcc ggcccgaaac acacaccttt ctgtgcccat atcgagaaaa
     2881 aacgcccctg atgattctgg cgcgactgca tggctatgca cggacatacc tggccgggat
     2941 tttagcggct aaaatccgat gaacgccctg aaagccacca caaaccaccc cgaaaacggt
     3001 gaacggggac gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg
     3061 ttttcagcac gttcaggaat cgggttcgtc gacggccggc aatcacgtta tggcaagtcg
     3121 ccggacgact caccacacga ttcaccggcg attcgactcg tgacttgact ctagagtcac
     3181 cgcggtcaaa aaaacgccaa agcgacgatt cgactctaga gtcaagtcgt cataagttac
     3241 tgatagtgtg gagtgagtcg tcgatgactc tatgacgagt cgtcggcaag tcgtacacga
     3301 atc
//
