LOCUS       NZ_CP065828             4444 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KpPF25 plasmid p3, complete sequence.
ACCESSION   NZ_CP065828
VERSION     NZ_CP065828.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17016749
            Assembly: GCF_016066025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Venkitapathi,S., Sharon,B.M., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Complete Genome Sequences of Six Uropathogenic Klebsiella pneumoniae
            Strains Isolated from Postmenopausal Women with Recurrent Urinary
            Tract Infection (rUTI)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Sharon,B.M., Venkitapathi,S., Ratna,T.A., Arute,A.P., Zimmern,P.E.
            and De Nisco,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2020) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 311x, 154x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:52:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 5,122
            CDSs (with protein)               :: 5,122
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 55 of 127
            Pseudo Genes (incomplete)         :: 65 of 127
            Pseudo Genes (internal stop)      :: 22 of 127
            Pseudo Genes (multiple problems)  :: 12 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065828.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpPF25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2018-09"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            join(4278..4444,1..91)
                     /locus_tag="I8N74_RS26670"
                     /old_locus_tag="I8N74_26670"
     CDS             join(4278..4444,1..91)
                     /locus_tag="I8N74_RS26670"
                     /old_locus_tag="I8N74_26670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_198091797.1"
                     /translation="MKENKKRNKTAKELAKITGKSERTIRSYFAQDRSSYEEEAKKRRL
                     KAYELKNNGLKLAEIATALGCSYNAAAGLIKRYKKQDLTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(160..1557)
                     /locus_tag="I8N74_RS26675"
                     /old_locus_tag="I8N74_26675"
     CDS             complement(160..1557)
                     /locus_tag="I8N74_RS26675"
                     /old_locus_tag="I8N74_26675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198091798.1"
                     /translation="MIHKIIGGKTKLNQRNIKNSISYLLREKKPAEQDLIRVLSGSKEQ
                     MLNFNSLIASKDLKTPYVAGVLSFEEENIPEAEKFKIMNDFEAMAFSGVPEEFRPPVLW
                     VEHRDKGRLELNYLTFNSLTTGRSFPVYYDKKDRRLFNDFTEVINYEGGYSSPFDDFTN
                     TQKIRMAEPPGKTLPAEKKALVEGLNNRLCSLILERKITNKADLITYLQEKEGFKINRI
                     AKNTVSIITDLDDTPIRLKGDIYQDGRDFGDYFKEKQAKPERTQAEISEKLSNHKPSYQ
                     KGLECRTERNRRVYIDPIIRRQQKEEELNKLTIKEEIKPEADTVAVTIPEVNNYDNKRE
                     LTESPATEIFRTIERIRDQQSKINTEQQFINKNTERSRSRIVELGNNTKGNRGRLGFFR
                     DYFDRFSCFINEKLRSVIEFAAEAERKLEEKTEQKQEVRVKKVKVKRRPQLGGRYRKHK
                     EDDELSN"
                     /name="hypothetical protein"
     gene            complement(1554..1955)
                     /gene="mobC"
                     /locus_tag="I8N74_RS26680"
                     /old_locus_tag="I8N74_26680"
     CDS             complement(1554..1955)
                     /gene="mobC"
                     /locus_tag="I8N74_RS26680"
                     /old_locus_tag="I8N74_26680"
                     /inference="COORDINATES: protein motif:HMM:NF017524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_142900245.1"
                     /translation="MREKRKIKRIEPELSPVSKNYKAKYKRKNMVSLCLNDDEYLKIEK
                     ITSALNVPRATASREILISNADDILSKIKRNDNKVLVLELNKIGNNLNQITKKINSNLE
                     LCLSGEGENFALAVDRLNETFNKILEAIK"
                     /name="mobC"
     gene            complement(2281..2808)
                     /locus_tag="I8N74_RS26685"
                     /old_locus_tag="I8N74_26685"
     CDS             complement(2281..2808)
                     /locus_tag="I8N74_RS26685"
                     /old_locus_tag="I8N74_26685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_103849472.1"
                     /translation="MSDIEDLNPDHMFDHLNSIFNEKTEDKEEEEKLFTEKELSDLFEL
                     FEEKMWEDLKKIKRECNQKNLQAYCSAALKNYKRNLVCSHIEKIKEVENLKITTRAADM
                     FFISYLGRSVGEKFLCANFGRDFKKGDQIILTDKYKGELNLIIAKYADECEKNLINIIS
                     LYRKLKNYIIYA"
                     /name="hypothetical protein"
     gene            3434..4276
                     /locus_tag="I8N74_RS26690"
                     /old_locus_tag="I8N74_26690"
     CDS             3434..4276
                     /locus_tag="I8N74_RS26690"
                     /old_locus_tag="I8N74_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_198091796.1"
                     /translation="MYEDNRQNFISQMPYKPYCSNDLTSGLTIRKREKALEMLYIQANQ
                     PTIQTCLLFDIDKENSFYTFEEAGLPVPQFITKSPDSGRCHYGYILRSGVCKTQQARLK
                     PLKYAAAVENGMADALGSDRAYVGLVTKNPLNSHWSPYWSGADLYELDFLADCVDLTVP
                     KQATKYENYGLGRNVNLFEDLRHYAYKHVLNFKKSANFEQFLNEVERQGINLNAVCNVG
                     NLLPFSEVKATVKSICRWTWKNFDSATFSAIQSQRAKKKSIKTKNKTKDLILKAMEDF"
                     /name="replication initiation protein"
ORIGIN
        1 attagctgag atcgctacgg ctttgggttg ttcatataat gcggcggcgg gactaattaa
       61 gcgatataaa aagcaagact taaccgctta attagctaat atgggggcta tataagcgat
      121 tatttagctc cttccttttg ggttgtttct tgatagtatt taattagaaa gctcatcgtc
      181 ctctttatgc tttctatatc ttcctccaag ctggggtctt cttttaactt taactttttt
      241 aactctaact tcttgttttt gttcggtttt ttcttcgagt tttctttcgg cttcggcagc
      301 aaattcgata actgatcgca atttttcatt aatgaagcaa ctaaatcgat caaagtagtc
      361 tctaaaaaat ccaagtcttc ctctgtttcc ttttgtattg tttccaagtt caacaattcg
      421 agatcttgat ctttcagtgt ttttattaat aaattgttgt tctgtattaa tttttgattg
      481 ttgatctcta attctttcaa ttgttctaaa aatttctgtt gctgggcttt ctgtaagttc
      541 tcttttattg tcataattat ttacctctgg aatagtaacg gccacagtgt cggcctctgg
      601 tttaatttct tcttttattg ttaatttgtt taattcttct tctttttgtt gcctgcgaat
      661 aattggatca atgtaaaccc gtctatttcg ctctgtacgg cattctaagc ccttctgata
      721 gctcggctta tgattgctta acttctcaga gatctcggct tgtgttcgct ctggtttagc
      781 ctgtttttct ttgaagtaat cgccaaaatc acgaccatct tgataaatgt cgcccttaag
      841 tctgatcggt gtgtcatcaa gatcggttat gattgaaacc gtgtttttag ctatcctatt
      901 gattttaaag ccttcttttt cttgcaggta ggttataagg tcggctttat tggtgatttt
      961 tcgctctaat ataagactac ataagcgatt atttagcccc tctacaagtg cctttttttc
     1021 tgctggcagg gtttttccgg gcggttctgc cattctgatc ttttgtgtgt ttgtgaagtc
     1081 gtcgaacggg ctgctataac caccttcata atttatcacc tctgtgaaat cattaaatag
     1141 gcggcgatct tttttgtcat agtaaacagg aaagctcctg cctgtggtga ggctgttaaa
     1201 tgttaaataa ttaagctcca atcttccctt gtctctgtgt tcaacccaaa gcacaggggg
     1261 gcgaaattct tctggaactc cagaaaatgc cattgcctca aaatcattca ttattttgaa
     1321 tttttcggcc tctggtatgt tttcttcttc aaatgacaaa acccccgcaa catatggagt
     1381 ttttaaatct tttgaggcta tcaaactatt aaaatttagc atctgctctt ttgaacctga
     1441 taaaacccta atcagatctt gctctgctgg ttttttttct ctcaacaaat aagatataga
     1501 atttttaata tttctttgat ttagttttgt tttgcctccg attattttat ggatcatttt
     1561 atagcctcca aaattttgtt aaatgtttca tttaaacgat caacagcaag agcgaaattt
     1621 tccccctcgc ctgataaaca caattcaaga ttagaattta ttttttttgt gatttggttt
     1681 aaattgtttc cgattttgtt taattccaac actaatactt tgttgtcgtt tcttttgatt
     1741 ttagacaaaa tatcatcagc atttgatatt aaaatttctc tcgatgctgt ggcacgagga
     1801 acatttaaag cagatgttat tttttcaatt tttaaatatt catcatcatt taaacacaaa
     1861 ctgaccatat tttttctttt gtattttgct ttatagtttt ttgagacagg cgaaagctct
     1921 ggctctattc ttttgatttt tcttttttct ctcacaaaca cactccgaat tatctttttt
     1981 cttttgattt tgacctggct tttggtgggg attgaagggg aggcgaagcc acccctcacc
     2041 agcgagcaat ccgaaggatt gattgtgctg gctctctaca ttaaaagcct tataaaataa
     2101 ataagtgata ataaaaataa gtcaacatca gattttaatt aagcgattaa atagcaatag
     2161 ctaataaaaa actatttaag cgattaagtt gccgaggggc ttaatagctg cacgagataa
     2221 aaaagcctta aatatcaata acttacataa tattattttt taaggctaac taagcgatta
     2281 ttaagcatat ataatataat ttttaagctt tctatacagt gagataatat tgattaaatt
     2341 tttctcacat tcgtcagcat attttgcaat tattaaattc aactcgcctt tatatttgtc
     2401 tgttaaaata atctgatctc cttttttaaa atcccttccg aagtttgcac ataaaaactt
     2461 ttctccgaca gacctgccta aataactaat aaaaaacata tctgcggctc tggttgttat
     2521 tttcaaattt tctacttctt ttattttttc aatgtgtgaa catactaaat tacgtttata
     2581 atttttcagt gcagcagaac aataagcttg taaatttttt tgattacact cgcgctttat
     2641 tttttttaag tcttcccaca ttttttcttc gaataattca aataaatctg ataattcttt
     2701 ttctgtaaat aatttttctt cttcttcttt atcttctgtt ttttcattaa agatagaatt
     2761 taaatggtca aacatatggt cagggtttaa atcttcaata tcactcatac acacctcttt
     2821 tttttagtta tagttattta atcgcttata tagtctatat atgatattaa aaaaataagt
     2881 caaatttatt ttgatgcttt tttttaaaag aaaaaacggg aaggggttta ttttccgaga
     2941 tgcggaactg atcgggggtt tttgatggca atcgtgaacg ggtaaaaatt ttaatttttg
     3001 cctgttcggg attaataagg tgtttaaggt taattaagcg attatttagc tataagatat
     3061 aatctttttt gaaaatgacg gcggcgaccg attttggttt actcaattaa attaaaataa
     3121 taataaaggt tttcttggtt taactcactt cgtgccatag tgaaaacata aaaaggcttg
     3181 cttgcctggc agggccaaac cccttaaaaa ggggctgtta tctgatatgg catattgcct
     3241 acttgacaaa gtaaaaaaag gcttgcttgc ctggcagggc caaacccctt aaaaaggggc
     3301 tgttatctga tatggcatat tgcctacttg acaaagtttt tttatgtatt acaacataag
     3361 aaaacaaaaa ccccctatgt tgttgaaagt ttggcgactg ttcaacatag ggggtaacat
     3421 aaaaggactt cttatgtatg aagataatcg acaaaatttt ataagtcaaa tgccctacaa
     3481 gccctattgt agtaacgatc tgaccagtgg cctaactatc agaaagaggg aaaaagccct
     3541 tgaaatgctt tacatacaag caaaccagcc aacgatccaa acgtgtctct tattcgacat
     3601 agacaaagag aacagctttt acacatttga agaggctggc ttacctgttc ctcagttcat
     3661 aacaaaaagt ccagactctg gaaggtgtca ctatggctac attctgcgat ctggcgtgtg
     3721 taagacacaa caagcaagat taaagccatt aaagtatgcc gcagccgttg aaaatgggat
     3781 ggctgatgct ttgggttcgg atcgtgccta tgtgggttta gtgacaaaaa acccgcttaa
     3841 ctcgcactgg tctccctatt ggtcgggtgc tgatttgtac gaattggatt ttttagctga
     3901 ttgtgtagac cttacagtgc ctaaacaggc cacaaagtac gaaaattacg ggcttggtag
     3961 aaatgttaac ttgttcgagg acttacgaca ctatgcctat aaacacgttt taaatttcaa
     4021 aaagtcggca aattttgagc agtttttaaa tgaagtagaa aggcagggaa ttaatttaaa
     4081 tgctgtctgt aatgtaggaa atttattgcc attcagtgaa gtaaaagcga ctgttaaaag
     4141 tatatgtcgt tggacgtgga aaaattttga tagtgctact ttttcagcta tacaatcaca
     4201 aagagccaag aaaaaatcta ttaaaactaa aaacaaaaca aaagatttaa tcctaaaagc
     4261 tatggaggat ttttaatatg aaagaaaata aaaaaagaaa taagacagct aaagaattag
     4321 ctaaaataac aggaaaaagc gaaagaacta tacgaagtta ctttgctcag gatagatctt
     4381 cttatgaaga agaagcaaaa aaaagaagat taaaagccta tgagttaaaa aataatggat
     4441 tgaa
//
