LOCUS       NZ_CP065780             9318 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Enterococcus faecium strain 16-021 plasmid p16-021_3, complete
            sequence.
ACCESSION   NZ_CP065780
VERSION     NZ_CP065780.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16988091
            Assembly: GCF_018517105.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother 76 (8), 1978-1985 (2021)
   PUBMED   33895846
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:34:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,862
            CDSs (total)                      :: 2,771
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 68 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065780.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="16-021"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p16-021_3"
                     /geo_loc_name="France: Caen"
                     /lat_lon="49.18 N 0.36 W"
                     /collection_date="2016-02"
                     /collected_by="NRC for Enterococci"
     gene            join(9039..9318,1..1847)
                     /locus_tag="I8F57_RS14075"
                     /old_locus_tag="I8F57_14070"
     CDS             join(9039..9318,1..1847)
                     /locus_tag="I8F57_RS14075"
                     /old_locus_tag="I8F57_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002289343.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            1844..2464
                     /locus_tag="I8F57_RS14080"
                     /old_locus_tag="I8F57_14075"
     CDS             1844..2464
                     /locus_tag="I8F57_RS14080"
                     /old_locus_tag="I8F57_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            2578..2715
                     /locus_tag="I8F57_RS14085"
                     /old_locus_tag="I8F57_14080"
     CDS             2578..2715
                     /locus_tag="I8F57_RS14085"
                     /old_locus_tag="I8F57_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            3157..3537
                     /gene="mobC"
                     /locus_tag="I8F57_RS14090"
                     /old_locus_tag="I8F57_14085"
     CDS             3157..3537
                     /gene="mobC"
                     /locus_tag="I8F57_RS14090"
                     /old_locus_tag="I8F57_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002319809.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            3519..4433
                     /locus_tag="I8F57_RS14095"
                     /old_locus_tag="I8F57_14090"
     CDS             3519..4433
                     /locus_tag="I8F57_RS14095"
                     /old_locus_tag="I8F57_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4393..4671
                     /locus_tag="I8F57_RS14485"
     CDS             4393..4671
                     /locus_tag="I8F57_RS14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002351557.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLTQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            4673..5362
                     /locus_tag="I8F57_RS14100"
                     /old_locus_tag="I8F57_14095"
     CDS             4673..5362
                     /locus_tag="I8F57_RS14100"
                     /old_locus_tag="I8F57_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002341840.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     FIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5483..5569)
                     /locus_tag="I8F57_RS14105"
                     /old_locus_tag="I8F57_14100"
     CDS             complement(5483..5569)
                     /locus_tag="I8F57_RS14105"
                     /old_locus_tag="I8F57_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            6213..6950
                     /locus_tag="I8F57_RS14110"
                     /old_locus_tag="I8F57_14105"
     CDS             6213..6950
                     /locus_tag="I8F57_RS14110"
                     /old_locus_tag="I8F57_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            6978..7517
                     /locus_tag="I8F57_RS14115"
                     /old_locus_tag="I8F57_14110"
     CDS             6978..7517
                     /locus_tag="I8F57_RS14115"
                     /old_locus_tag="I8F57_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            7533..7703
                     /locus_tag="I8F57_RS14120"
                     /old_locus_tag="I8F57_14115"
     CDS             7533..7703
                     /locus_tag="I8F57_RS14120"
                     /old_locus_tag="I8F57_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(8017..8478)
                     /locus_tag="I8F57_RS14125"
                     /old_locus_tag="I8F57_14120"
     CDS             complement(8017..8478)
                     /locus_tag="I8F57_RS14125"
                     /old_locus_tag="I8F57_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            8611..8985
                     /locus_tag="I8F57_RS14130"
                     /old_locus_tag="I8F57_14125"
     CDS             8611..8985
                     /locus_tag="I8F57_RS14130"
                     /old_locus_tag="I8F57_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     BGC             3611..9318
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6214..6950
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3520..4430
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattttctaa aaaaaatata ttagcaagaa acgattctaa agtccattat actaataaca
       61 aaaattctaa gtaccaatta aaacttccaa ataatttttt aaatataaat atttatcctt
      121 tttctcatgt ggaaaatatt ggattaagtg aaatattata tgtacaagga aactcaaaca
      181 aattaattcc tgtactgaaa aaatatggaa cacctactat ttccaatatc aatcaatcag
      241 atacttttca aattaatact aatattgttc ttgttatctg ctatcttagt atatttattg
      301 taattactac aattatattt gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt
      361 taggctataa ctcttttgat actataaaag attctattaa agagttaaac ctcttatttt
      421 gttctttgat tataactttc attttatcta tttttttgag catatattac agtaacgaaa
      481 attttttcct atcatttctt ctatgtagta tttttcattt tataattagt tttttattta
      541 tcatagttac tctaagtatc tatcatttat ctagtataaa aaattatcta aaaaattcac
      601 gaccacttaa gttaaatctt tgtatattaa atatctgtct attcttaagc atgatccttc
      661 tgttaatagc ttctacaaaa gtaataaata tccataatga agccgaaaat aattctctta
      721 aatattggga aagaactact aatttatata aaacaaatat tactaatcaa ttaaatagaa
      781 acaacaccgt agaagaaaat aactacttga aaaaagctag taaatttgtt tataaaatac
      841 agaagaatta taaaactttt attatcgcgc catacaatta cgcaactatc caagaaaata
      901 ataaagaatt ttttattgga caaaaagttt atccaaattt tgaagactat gttagtcaac
      961 ctgctggtgc aggaatatgt gttgatttaa actatctaaa atattacaat cctatttctt
     1021 ttgaagaatc tactgactta aatttaataa actctgatcc actaactact tacattctag
     1081 tacctaaaaa atataaagaa tatgcaaaaa tgattgaaaa aaattattta gaagacatac
     1141 agtttagact tgaagactct cctcctcaag ctccttataa aactcctaat ttgaaaaatt
     1201 taaaaataaa actaatattt gttaataata accaaaagta ttttagtttt aataccttat
     1261 atggaaaggc aaaagataac tatacaataa ctgatcccat agttagagtt attaatccag
     1321 aaataacaga atcattattt tggggaaata tattgactag tgatggtgga ttattttttg
     1381 atcagaaaca tacttcaaac caaaactttt ttaatcaaat taatccgtat ataaaagaat
     1441 ttaacctaga aaatatcata aatttttctt attctgtttt tcaagaaact ggtgaattat
     1501 tatctcaaaa aaaagtaaat aaattaaatg catatctaca atatctttta atattaggat
     1561 tatttataat aattaatttt caaatgattt ttgttagttt tactataaat tcaaaaaaaa
     1621 tagctttaaa aaaaatattt ggatattcac caataagtca agttttagat ttatgtttac
     1681 tcccttttac aatagagtta tttgctacta tgtgttttca attgatattt tcaaaaaaca
     1741 cttacttgta ttttataatt tttatgatct ttttaataaa tctaattact tctattatta
     1801 tttataaaaa aaatagtttt aaatatttta aggagaatat tttatgatat tatcagttga
     1861 gaatttcacc ctgtcaatta ataaaaaaaa gatcaaacaa aattggaact tttcaatatc
     1921 agaaaaagat tttattatag taacaggaaa aagtggtagt ggaaaaacta ctctgttaaa
     1981 taatttaagc ctaatagata aacaatatac cggtaactta ttctattttg aaaagaaagt
     2041 ttctaaacat gatatacaac gtctacgaag aaatcatatt agctatttat ttcaaaacta
     2101 tgctcttctt gaaaatcaaa cagtacaata caatttcgaa ttagcaaaaa agtttaacag
     2161 taatatttcc aatgatcata tttataattt attaacagat ttttctttgc caaaagatat
     2221 cttaaaagaa aaaatctttc tcttaagcgg tggagaacaa caaagaatag cattaattag
     2281 aaatatgtta aaaccatttg atatattatt tgcagatgaa cctacaggaa atttagataa
     2341 agaaaactca acttttataa tgaactttct gaaaaattta acagaaaaag aaaaaaaagc
     2401 cgttgtacta gtaacacatg acttaaatgt tctaaactat ggaactaata ttatagaatt
     2461 ataattttta cccgtgatgc tagctgattt aaaactagca ccacgggtat ttattttata
     2521 ttccatctct cctatcccaa aatttaaata atcacttaat aaaaaggagg cacccaagtg
     2581 attttattga aaaaagagta tgaaaaattt tatgatagac ttatttattc tttgcaattg
     2641 cccaaaaaat taaaccgtca attacaaaag acttatgaaa ctaaatcaac tgaagaatgg
     2701 gagagttatt gctagatttt tggtagaagt aacaaaaagt ggacatatta tcataatgac
     2761 tcgccgaaag tctgataaaa aattggctaa aaatattgct gaatgtttaa taaaactatc
     2821 agttttagct aaatagttag tagtttacac aaaaaagcat ttatgttttt tgttgcaaag
     2881 caacaatttc atcttcggtg aaataagcga aactttacaa aaagtggcta gcatttgcta
     2941 gctttttttg tattgtggag cgtgggtttt gagggggatt caagggggag cttgcgcccc
     3001 tttgacaagc ggttatttgc accaaatacg gaggtatttg gctagcaaat atagagcttg
     3061 ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac
     3121 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa tcaaaatttg
     3181 gctagcaatt attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     3241 gtgagcgaat ccgaatttgc aaagctgaag caatcggctg aagctttgca aatgagtgtg
     3301 ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     3361 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
     3421 gctaagtggt gcaatacaca ccaacatgac gttgcttctg gtgacgctca acgtttatcc
     3481 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacaatt aaggtaagcc
     3541 gaaccacttc ttgtagtcgt gcaattaatt atgcagaacc acgagcaacc attaaaacag
     3601 gaataaactg tgacgtcgat tatgccaaaa gtgaaatgaa acaaattcgc atgctgtatg
     3661 gaaaagacga ccgtgtacag gcacacttat tgattcaatc ttttcgacca ggagaaatta
     3721 ccgctgaaaa agctaatcaa ctaggggcag agtatgcaaa aaaaatagcc cccgatcacc
     3781 aaatagctat ttacacacat acagacaaag accatattca taatcacatt gtcattaact
     3841 ccgttaatct ggagactgga aaaaaatttc aagctcatgg tcaagcattt ctaaacaaat
     3901 gttatgacct aaatgacgag atttgtcttg cacataactt accaatcact gagcgaggaa
     3961 agaagccaga aaaacgaacc atgtctgaaa taaaattaaa agaaaaaaat gaacctgttt
     4021 ggaaagacga aattcggatt gcgatcgatc aaacaatgaa aaacaaaaaa accagaacct
     4081 atgaccaatt ttgtaattcc ctcaaaacat tggggattca ttgcttcaat cgcgggaaaa
     4141 atttcactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaga ctaggaaagg
     4201 actacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc tataaccaac
     4261 agcgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac
     4321 gaagagaaca acgaactctt acgcctgaac aaccggaaat tagaccggct cgaagaaacc
     4381 atgaacaaca cgttgaaaga ggaacagacc accaacaaga attatcgctc taaaacgtta
     4441 aaagacatag aaaacgaaat aaagaaacaa cgtgcttttc tcgttcctta tacgcaagaa
     4501 aggatacaaa ctacgattga tttacaagtt aaaaaactcc atcaagagct attagaccag
     4561 cgaaataagg aacagaaaaa ggtgcttact caacgatcat tggattttct tgccccctta
     4621 tgttgcttag tcctgacact gttcgcattt tggttagtat ggggaggcta aaatggcatt
     4681 ttatttaccc tatctactca tttttgttag tatttcagga agtatttggc tcatctataa
     4741 aatttttcaa acaagatatt cattaaaagg ctctaaaatt cgatttaagc gatttttctt
     4801 tcttggcttt atattttact tgataatcgt ttcaagcggc ttattcggcg ttttagaggg
     4861 aaataagcgc gtttctagaa gtattttatt aggaaatgtc actcaaaagt atgaatcagc
     4921 gcgtgataaa aagaaaaaag agcaagcatt agcacaaaaa atggaagagt ttaccacttg
     4981 ttatgaggaa atgaacgata tttttgtgaa tcaagaaaaa cgtttgacgg ataaaaacat
     5041 ggaaaaactc actcgtcttt atcaaaactt gccagaaaaa cagcaaaaag aggttcaaga
     5101 caactatgag cgaaccaaaa aagacgtgca atatgtaaaa gatacaaaga tagaagaagc
     5161 ctgtagtgat ctatttggag acacaaaccc ttggtttgcc agtgaagaag aaaaaaaaga
     5221 aaaacaacaa tctgtcacat atgaacgcta tgaaatcttg cttcaacaag caactaacat
     5281 gcaaaatccg acaaaaaaag aaaccgcctt aaactatcta aaaagtgtca aggaatggtt
     5341 agatcagcaa caacaaaatt gaatttgatt taaaatcatg ttaaaatgaa tgtgcaaatg
     5401 atcaaacatt agatacacga aagccccaga gaggtcggat tctctggggc tttttttcag
     5461 gttgatgtcg cctttattga ttttacttct tgtttcggtt attcaaccaa taactgaata
     5521 aagagacaca aactcctaca agaagggggc aaatgaacag ttcaaacatt agatacacct
     5581 ccttccagtc gcaagactgt cggataggcg acaagttatt atatcttaaa aaattaaatt
     5641 tgtgtggtgt ttttaagtat cacttgtttt agggttgtta gggcttgccc tgaccgcctg
     5701 taaggcgctt ctcgactaga ttaagggaat taatcaagaa ttatcttaga acggcttaga
     5761 caagctttca gagcctcaca cctcctcttt gaacaacggt tttagcaaag gagccggagg
     5821 cgactgcgca gtataatatg agagcatagc gatcacacct ttttagcgac cgtagggagc
     5881 aagttttcca gtttagaaaa attcgcgaac ctttttggag gaaaaagtag gagtttttta
     5941 ggcgggaaat tcaatgtgct tttaagatat actgttagat atactgttag atatactgtt
     6001 aataataact gttagggatt agcaacctta tgggagagta agtattgaca taggtagtgg
     6061 gggataaatt gtcacactat gggggataaa ttgtcacact atgggggata aattgtcaca
     6121 ctatggggga taaattgtca cactatgggg gataaattgt attttacatc ctcttatgtc
     6181 tatgataaca taatttattg aggtgatttt tattgagtaa cgatatagtt cgatataata
     6241 atggattaaa ttctgtccct ttaagaaaat ttacccctat agatatggat ttattttggg
     6301 gaatatgttc taaaatgaaa agaaaaggta caaaagaaga aatttttaca tttaaacaaa
     6361 taaaagaatt aattgattac aatataaatg cacgtggcga ccgttttgct aatgatttaa
     6421 taaaaatgtc aaaaaaaatc attactttgt cttttattta tgaagacgaa aaaacatatg
     6481 aacaattaaa tctgtttcaa aaatttaaaa tcgataaagt gaatgaaaca ttattaataa
     6541 aggtttctga agaatttgaa tatatactta attcaatcgg aactaatttt actcgtttcg
     6601 aattagaaaa tatgacaaac ttaaagagta gctacgttaa agagttatat cgccaactta
     6661 tggctcataa agacagaaga tctaaaaaag gtcactggtt tgtaaaaata gaagatttta
     6721 gagctctatt agcaatacca gaaagttatc gcatgagaga tattgataaa caaatttttg
     6781 cccaagcgaa aaaagaattt ttaaacgtta attcaactca ccctgctatt tttaaaactt
     6841 ttgaaatcaa aaaaattaaa gcctacaaag gaaataagat aggatctctg caaatttatt
     6901 ttgaagaaaa tataccttca attaccttag ataattggtt agagaaataa tacctaacaa
     6961 tttaggaggc aattatcatg caacccaaag aaacctttac aataaaggaa ttatctgaat
     7021 tatttgaaat aagtaggcaa gcaatctcta aacatgttga taaattagac ccgtccttat
     7081 tagctaaaaa tgagcgtggt tataaggttg tattacgttc tggagtattg caacttgcta
     7141 gcaacttagg taaccaaagg ttactatata ggttgcatga gtatgaacaa gaaaacgata
     7201 aaaacgttta tatatcaagc aaaaacgtga ttgtaaacga gttgctagat aagttgcgtg
     7261 aagaaaatga atttttacga gaacaacttt ccctcaaaga gcaaaataat tctaatatgc
     7321 aaaaattact cgaccagcaa caacagctca ccctacaggc aaatcaacag atcgaaaaac
     7381 tgcaagaaca gttacaatta gcttatacgg aagaaactcc agaagatgaa tcaactacta
     7441 ttctttctga agaaacagaa tcgatagaaa aacacacaaa aaagaagaag caaaaatggt
     7501 ggcaactttg gaaataactg gagggaacat tcatgacaag aaaaaatctt ctattcggtt
     7561 ctattttact aggaattcta ggattcagtc cgatgttatt gtcagtaatc aataaaggga
     7621 ataaacatgg tgaattgaaa ttgaatccaa attattacaa cagtcctaac gaagaattgt
     7681 ttgtaaaaga gacggataaa taataaaaaa gactgctctt ccgagcagcc agttaaaaaa
     7741 ataatgagag tttattcttg aaaaagcaac gcgcttataa agcgctacca aaattttatc
     7801 atattttata aaaaataaca atccagtaaa aaatgactgg taaaaattca caaagaaacg
     7861 gaaagaagca actacagaaa atcaagcctt aaaaagagct ttttacctag accaattaaa
     7921 aatcacaaag tgagcgtctc tatccattgg tagaggggca acttggtcta gtgtacatta
     7981 tgtcaactat gcaattaaat aactaactac ttcatattag cttaataaaa taatcaaaat
     8041 cactagggga aaaaatattg tataaaaaaa tttttcattt tttaaataag aatagtaaat
     8101 ataaaaataa tttaagatat ctattactat taatccagat agagcaaacc aagtaaatat
     8161 tccgtgtgta aagctaaaaa aattcatttg aatcaattca aataaaccta atatagtaac
     8221 aaatatatta aaaataatag ctgtcaatac cagtaaatta ttttgtttat tttttaaatt
     8281 atcttcattt aaaatagaat ttatgttagt actataaaat tcacaaattt tttttagtgt
     8341 ctctatatct ggtgttgttt tgttattttc ccactttgaa atagtttgac gtgtaacatt
     8401 aaattttctt gccacatatt cttgagtata cccttttttc actcttaatt ctcttagctt
     8461 agatcctaaa tcctccattt tttctcctat gtaaacgatt atttacataa aattttatca
     8521 taaaaatact aacgcgccaa aaaagttctt tattttataa aacacactaa aataaaataa
     8581 ttctagtaaa aaagatagga gaaataagtt atgaaaaaat ttttatgtct ttctgcttta
     8641 atgggtgtct tattaactag tggaggaata gttagtgcta cagaagcttt aaatctagat
     8701 gtacccgaag accataccgc catttacgga ggaggaatgg aagctacaga agaaggattt
     8761 tcttctagta aattgagata tgctgctggc ggcggagatt tcaactgtgg ggttaatgga
     8821 tttaaagttt atgcaaatta ctaccatgct agagctaaac atagtgcaac agctaaaaat
     8881 ggacgaggcg gtcaagtacg atcagttcag aaagctggag taagagctta tgcaacatgc
     8941 aatgccacac ttactggaaa tactggttgg tggaatgttt attaaaaaag agagtttaaa
     9001 ctctcttttt tatttacatt tgaaaggaaa ttcaaaatat gttaaaaaaa ataaacgctg
     9061 ttttatcact ttttgtctgc attacaatac tattcttttc tactaatgtt ataaatggta
     9121 taaagacttc tgaagcgcta tataaaaata ccgctgaaat tcatatgcaa tataaagatg
     9181 atatggactt taacagttat ataaacgctt tgtatgaaat atctacacaa gaaaatatta
     9241 atatttctca atatagtttt actgctgtta accaactaac tattcttgct acaaacccta
     9301 acgctaacaa acactgga
//
