LOCUS       NZ_CP065762             2056 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Enterococcus faecium strain 18-276 plasmid p18-276_8, complete
            sequence.
ACCESSION   NZ_CP065762
VERSION     NZ_CP065762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16988099
            Assembly: GCF_018516925.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother 76 (8), 1978-1985 (2021)
   PUBMED   33895846
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:29:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,703
            CDSs (with protein)               :: 2,703
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 36 of 102
            Pseudo Genes (incomplete)         :: 68 of 102
            Pseudo Genes (internal stop)      :: 22 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065762.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-276"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-276_8"
                     /geo_loc_name="France: Corbeil-Essonnes"
                     /lat_lon="48.6131 N 2.4659 W"
                     /collection_date="2018-05"
                     /collected_by="NRC for Enterococci"
     gene            complement(join(1732..2056,1..278))
                     /locus_tag="I8F55_RS14355"
                     /old_locus_tag="I8F55_14360"
     CDS             complement(join(1732..2056,1..278))
                     /locus_tag="I8F55_RS14355"
                     /old_locus_tag="I8F55_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(345..500)
                     /locus_tag="I8F55_RS14360"
                     /old_locus_tag="I8F55_14365"
     CDS             complement(345..500)
                     /locus_tag="I8F55_RS14360"
                     /old_locus_tag="I8F55_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1117..1338
                     /locus_tag="I8F55_RS14365"
                     /old_locus_tag="I8F55_14370"
     CDS             1117..1338
                     /locus_tag="I8F55_RS14365"
                     /old_locus_tag="I8F55_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            1339..1596
                     /locus_tag="I8F55_RS14370"
                     /old_locus_tag="I8F55_14375"
     CDS             1339..1596
                     /locus_tag="I8F55_RS14370"
                     /old_locus_tag="I8F55_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     Typing          1499..1674
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(1805..2056)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="3.99e-129"
ORIGIN
        1 tttgcattag cacatttttg cgggtttgga gaccgaagct tttgagttat ctcacgtatt
       61 tgatcatacg tctttttacc gctaaacatt atcaaaacat gccaatgagg ctttttcaat
      121 tcaccgtcag gattcacatc tttatcatga agtggactct caacccaagg aacatgtaac
      181 tcatctaaaa actccctcca attttcagga gccgattcag gatagactac aaacgtccaa
      241 ttccttgtgc gttcatcttt agtttttcta ctcgtcatgc tataattacc ttaattgaac
      301 aacagtctaa cagcaaaagc agtgcgccaa cactaatgct tttgctattt tttttgctca
      361 ttttcctttc cgttagcatt aacccaaatt gtaataagac cagacttact aagacccatt
      421 tgtttcgcaa ttttatcaag attatctgcc acttccttag taagcgttac aacgactctc
      481 tttttttcat tactgatcat aattcgtatc ctccaacgac tttttcttaa aaaccatagt
      541 aacaaaaaag taactaaggt gcaacagttt agatactaat tagcgaataa taggccttaa
      601 acccttggca ttactgattt tgtcaaaaaa aaacgctgaa acccttggga gagtaaggct
      661 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      721 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      781 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      841 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      901 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
      961 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1021 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1081 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1141 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1201 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1261 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1321 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1381 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1441 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1501 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1561 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1621 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1681 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1741 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1801 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1861 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1921 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     1981 atgagcgata atatcacttt tagcatattg aaatttttct ggattatcca tatgagcaaa
     2041 atatcgcacc atacct
//
