LOCUS       NZ_CP065761             3199 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Enterococcus faecium strain 18-276 plasmid p18-276_7, complete
            sequence.
ACCESSION   NZ_CP065761
VERSION     NZ_CP065761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16988099
            Assembly: GCF_018516925.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother 76 (8), 1978-1985 (2021)
   PUBMED   33895846
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:29:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,703
            CDSs (with protein)               :: 2,703
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 36 of 102
            Pseudo Genes (incomplete)         :: 68 of 102
            Pseudo Genes (internal stop)      :: 22 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-276"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-276_7"
                     /geo_loc_name="France: Corbeil-Essonnes"
                     /lat_lon="48.6131 N 2.4659 W"
                     /collection_date="2018-05"
                     /collected_by="NRC for Enterococci"
     gene            290..514
                     /locus_tag="I8F55_RS14330"
                     /old_locus_tag="I8F55_14335"
     CDS             290..514
                     /locus_tag="I8F55_RS14330"
                     /old_locus_tag="I8F55_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1564..2283
                     /locus_tag="I8F55_RS14340"
                     /old_locus_tag="I8F55_14345"
     CDS             1564..2283
                     /locus_tag="I8F55_RS14340"
                     /old_locus_tag="I8F55_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(2420..2836)
                     /locus_tag="I8F55_RS14345"
                     /old_locus_tag="I8F55_14350"
     CDS             complement(2420..2836)
                     /locus_tag="I8F55_RS14345"
                     /old_locus_tag="I8F55_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2839..3123)
                     /locus_tag="I8F55_RS14350"
                     /old_locus_tag="I8F55_14355"
     CDS             complement(2839..3123)
                     /locus_tag="I8F55_RS14350"
                     /old_locus_tag="I8F55_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          1565..2283
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaatgctat acataagcta cccacgatgg cgaaaaagtg atatcaaatt tttttctgag
       61 aaacgtgata ggcacagcct atctacccac tttttttaga aagtgggtac acgtttcctt
      121 atgctcttta aaattcgggg gggagtgctg cacgtgtaat cgataaccaa atttaaaaat
      181 gcacgatcaa aaatttattt agtttttcaa acgacatttt ttttgagagt atagacggca
      241 aagaagtgat tttatttcta gaaaaattgt gagactggag aaagaaaaaa tggaaaaatt
      301 tccttacgaa tattttcaaa acttagaaaa attagagcaa gcaattcaag caatcaagtt
      361 cgatttaaca gacgaaaaat tgttggaata taaaaatatg acacccgaaa gaaaacgatc
      421 gttggaaaaa ttaatgcaac tagtgaacga agacggcgga atttcagacg cagcttttga
      481 cgcatggagc gaatttcatg gagaatataa ttaagtgggt ttaaatggct ctaaaatcgc
      541 ttctaagagt tttttagctt ggaccaatga aatacaccaa aaactaaata aaacgtctta
      601 aatcgaaaaa tacgagctct cagagcgact ttatttgtaa atgttgcata taagaaataa
      661 ttacggattt tagacttttg taggatattt ttgaaataaa tatttatttc tgttataatt
      721 tgattgcaaa taagtgaaca cgaaacacac gaaagcccca gagaggtcgg attctctggg
      781 gctttttttg tgggctgagt cgccttaatt aagttttact tcttgtttcg gttattcaac
      841 cagtaactga acaaagcgct taaaagccca acaagaaggg agcaaataaa gttagtgaac
      901 acgaaacaca cctccttcca gtcgcaagac tgtcggatag gcgacaatca attatacaat
      961 aaaaaagcaa aaataagcct aagtttttta ttgccttggg gttgttaggg cttgccctga
     1021 ccgcctgtaa ggcgctttag aacgaaggac atagttttta tcgaatcttg tcttagaacg
     1081 gcttagacga gctctcagga cgccacaaag gagtccctaa tgaattattt ttggcgaagg
     1141 agcctaaagg cgactgctgc aagtaaaatg tgagagcgta gcgatcactc cttcttacag
     1201 cgaccatagg gagctagatt ggcagtttaa tatcatgcgc gaacctgttt attttaaaaa
     1261 gtgggtgatt ttttggcggg aaattttgct cgcgcgcgcg cgtttatttt atttattttt
     1321 taaagatttt ttagaaccct ttttaggggg cagttgagcc ttactctccc aacggataca
     1381 gaagggtact aacaactttt tatctgagta ctaacaactt tttatctgag tactaacaac
     1441 tttttatctg agtactaaca actttttatc tgagtactaa tatccttttg tataataaca
     1501 accaacctat ttacgagttg gttgttaaag gttattattt atttgtaagg ggggaagaaa
     1561 ggaatggatg aaaaaacgga attaacagtc aaatatcaaa atgaattgaa tttagtaccg
     1621 ctgaaaaatt tcaatgctaa gcagatggat ttattttttg cattatgcgc tcgtatgaaa
     1681 gatcgtggtt tgaaaaaagt tagattttct ttcgaggaat taaaagagtt aagtgattat
     1741 aagatgactg ctacaaaagc ctttgtcggt gatttagaaa agctctataa agatatgctt
     1801 aatcttactt accgtactga agatgaggat tctattcgct actttgtttt gtttactggt
     1861 tttgaaatca ataaaacaaa aaagtttgtt gaagtcagtg ttaatccgga cctagaatat
     1921 attattaatg gtttaacagc tgaatttagt agatttgaat tatctgcttt cacgtcgttg
     1981 cgttctacgt atgcaaaaac tctattccga ctgcttatgc agtaccgttc tactggttat
     2041 tatgtagttc gtattgaaga gtttaggagc ttaatggata tcccagaata ctatcaaatg
     2101 ggtaacattg atcaaaaagt attaaatcct gcattacgag aactaaaaaa atacttcaat
     2161 gatcttaaag tcacaaaaat aaaagcaaaa aagggcaata aaatcgccaa attagaattt
     2221 actttttcaa gcctaacgtc agatttgcca aagatttctc tgcgtaactg gttagaagaa
     2281 taaataaaaa agaactcgcc ccccttacat taagcaagta gacaagtccc ttacaaccga
     2341 ctaagattgc tagtacattc tagcatattg tatggggctt ctaaataaaa aacagatagg
     2401 aataactcct atctgttttt tatcgtttta gaaaatttgt tgggtcgaaa tttctagaag
     2461 tgactgtttg actggtttct ttttgatgat tctttggtcg atttcgattt gatggattta
     2521 gtttagcttc gattttttct ttcacttctt gatatacttt agaatttgtg ccaataagaa
     2581 cgcctgtatc ttttagatat aaatcagcaa attctgcgat gatttcagtc atgatggaaa
     2641 tttcttttcc catagcattt agttttgcct gattgctggt agtggtcgtg ttggtatcgg
     2701 gttcataact catacataaa aagcgaatgg cttccgagta gttattgata tctggatttt
     2761 tttcgatgat ggaatctaaa acgtcaacat gactagggta caaagtaaca tgtttttgga
     2821 cagaagtttt tctcatggtt atatcattcc tccgataagc atttcaatga tttgattatt
     2881 tgtgtagaca tactcgctta atatttgagt attttctgtt tggatagcaa taaatttatc
     2941 gagcaataaa tcgaattggt cttttttgta agtcgtaagt tgacggtcaa aatcactttc
     3001 gattaaaact tttagatatt tatttcgact ccatttttct cctgttttat tcgtgagttc
     3061 ttggcaacgt ttgtctattg ttgcaacaaa ctttggatca atattccgaa ctaaaatgtc
     3121 cataaaaaat ctccttttgc aattatgtat aatttatgta tatcatacaa atactatatt
     3181 tgtatagctg ttttttgcc
//
