LOCUS       NZ_CP065758             4466 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Enterococcus faecium strain 18-276 plasmid p18-276_4, complete
            sequence.
ACCESSION   NZ_CP065758
VERSION     NZ_CP065758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16988099
            Assembly: GCF_018516925.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother 76 (8), 1978-1985 (2021)
   PUBMED   33895846
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/18/2023 05:29:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,703
            CDSs (with protein)               :: 2,703
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 36 of 102
            Pseudo Genes (incomplete)         :: 68 of 102
            Pseudo Genes (internal stop)      :: 22 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-276"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-276_4"
                     /geo_loc_name="France: Corbeil-Essonnes"
                     /lat_lon="48.6131 N 2.4659 W"
                     /collection_date="2018-05"
                     /collected_by="NRC for Enterococci"
     gene            complement(669..1103)
                     /gene="bacA"
                     /locus_tag="I8F55_RS14270"
                     /old_locus_tag="I8F55_14275"
     CDS             complement(669..1103)
                     /gene="bacA"
                     /locus_tag="I8F55_RS14270"
                     /old_locus_tag="I8F55_14275"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(1509..1682)
                     /locus_tag="I8F55_RS14275"
                     /old_locus_tag="I8F55_14280"
     CDS             complement(1509..1682)
                     /locus_tag="I8F55_RS14275"
                     /old_locus_tag="I8F55_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2367..2906)
                     /locus_tag="I8F55_RS14280"
                     /old_locus_tag="I8F55_14285"
     CDS             complement(2367..2906)
                     /locus_tag="I8F55_RS14280"
                     /old_locus_tag="I8F55_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(2922..3845)
                     /locus_tag="I8F55_RS14285"
                     /old_locus_tag="I8F55_14290"
     CDS             complement(2922..3845)
                     /locus_tag="I8F55_RS14285"
                     /old_locus_tag="I8F55_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     Typing          complement(2923..3845)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaaacgca aattagagct tgataaagat atttgctcgt gtgcctagac ttttaaaaaa
       61 gtcggcacac gccatgacaa agctgaaata aactgcttca gctttgtcat ggcggattcg
      121 ctcacttgaa aaagtgttta tttcgctcga ttattagcat atatttttta gcttttttgc
      181 tagccaaatt ttgcattaat ttgctcataa gtcccaccct ttcagtacgt cctacggacg
      241 tatcgttcgt aatgtgaaag tacctttaaa ggtactttct acaaaactca ctaaaagggt
      301 ttggcaagct ctatatttgc tagccaaata cctccgtatt tggtgcaaat aaccgcttgt
      361 caaaggggcg caagctcccc cttgaatccc cctcaaaacc cacgctccac aatacaaaaa
      421 gtggctagaa aatgctagcc actttttgta aaatttagta tgatttattt ttagctattt
      481 agagttttta tttccgtaaa tacttctaaa aattgctaag ataaatgcta taggaattag
      541 ataaagaata cttttttgta agcttatatt gaaaattagc ctaaatatga agaacatagt
      601 gattaaaact attattgaaa taattacagc taaaatcttt ttattcatga gatagtttca
      661 ctttctgatt attttattac ataacgagcg taatcattgt attgatatga tgttgtattg
      721 ctaaagtgag cattatatgt taattgtaca ccttttcctt gatttgcagc tcttacaaag
      781 acattcattg catcaacagc actactaaaa gcatttgtaa taacccatgt tccaactcca
      841 ccaataggaa taaaagatgc aatttgagca ggagtcttta gcttattata attttttttc
      901 aaatctctac acattttatt agtaacataa tagttcattt tatagcaagt aaatccacga
      961 tgattatgat tatatcgtga acttgcagct tccactttta tacctctagc actaaaagga
     1021 aacagattta acggagtact tgctccaaca atcataccga acaaaaatag acaagtaata
     1081 attttaataa ttttattttt cattgttact tcctccttag atataaaatc ctaggaaagc
     1141 gctttctgta tatatttaga ataatcagaa aattaaatat tgtcaagagg gtttatttta
     1201 taaaacgtgt tattttaaag gttagtagac ataatgtcga ctagaccaag ttgcccctct
     1261 accaatggat agagacgctc actttgtgat ttttaattgg tctaggtaaa aagctctttt
     1321 taaggcttga ttttctgtag ttgcttcttt ccgtttcttt gtgaattttt accagtcatt
     1381 ttttactgga ttgttatttt ttataaaata tgataaaatt ttggtagcgc tttataagcg
     1441 cgttgctttt tcaagaataa actctcatta tttttttaac tggctgctcg aaagagcagt
     1501 cttttttatt atttatccgt ctcttttaca aacaattctt cgttaggact gttgtaataa
     1561 tttgaattta atttaagttc tccgttggaa ttgttttttc caattacgga taatagcatg
     1621 gggctaaaac ctaatattcc tagagaaata gaacccagta ccaacacagt tttttttaac
     1681 ataggttata cctccacttt ttttaacgct accacttgta tacacatttt aaatactagc
     1741 cttcagttta tcaaatgttt aaattgaacg gatataaatc aaaattaaat tttcaaaagt
     1801 tgttctaaaa aatttttctt ttttggaagt tactctaact ttttttgctt gtggtgcttg
     1861 gtatgttttc ttcgccgacc gtttaggttc ttgaccattt tctcttataa gtgggatttt
     1921 taccacgttg agagaaaatg gtcaagaacc tgggaggcgg agaatgccct taataccaag
     1981 caccgcaagc ctttcccata ctttattcga tgatcccacg atgatctcat aatgattcta
     2041 caatgaccct acgatgatca aaattctcaa aaaaaataaa cgaaatttca aacgattttt
     2101 ttgaaatttt gttaaaatca catttgtttt tttatgactt tttcaacaaa atcgattaga
     2161 atttattttg tttttccacc aaaaattgag tgagatttca acctatttta agcaataagt
     2221 caccatatag tttgatagtt tacaactata aacattgatt gtttacagtt gtaaactatt
     2281 ttttctttct cataaaaatt tgagtgatct ccccatcagg tagtacaact ttttcctcta
     2341 ctatccgaac taacttctag aaaattttag agattggttc tattagattt ccaaatttgc
     2401 caccactttt tttcaataag tggttgtgta ttgtctgctt ttttttcaga aaaggcagtt
     2461 gattcggttg ttggagtttc ttctgtataa gttaaatgga gctgttcttg taggttctca
     2521 atttgttggt tcgcttgtag agtgagttgt tgttgctggt cgagtaattt ttgcatttgt
     2581 tttatttgat tatctttttc ttgtagctgt atatcttttc gatttaactg tttttctagg
     2641 aaagaaagga gttttaattc ggaaacttgg ttggtgtttt tatggttttt ggtttttgat
     2701 ttttcgccaa ctagttttcg atttaagttt gcgttatcga ctaaaaacat tgattttata
     2761 gctttttgac cggtttcgtt aatcacaaac ttgtttccgt tttttgcaaa ccaaagtttt
     2821 cgatttttgt cagaaacttt tttgttaatt gctgttttac taactcctaa ttcttccgca
     2881 agttctttga ttgttttatc tgccataaat actccttaag cttaatcgaa taagttttgt
     2941 aaatttccta gtaaatcttg tttgagtttg tttgaaagtt ctcgtttttc tgcctgtttt
     3001 tcttcagttt gttgttgttg tttaaaatca gcttcatgag tgcctaaggg aagatttagt
     3061 attcgatctt tagcgcgcca tttttcttct cgtgttaatt ctccattgtg atcaatatta
     3121 aataatttta tacgtaaatc ttcttgtttc cctttggaaa aatcgtcagc atggttaatt
     3181 tcagctttcc aagtaaaacg ataaccgata acaggtttac ctcgaccttt accatatttt
     3241 ttacgaatag ttaatccttt aaataaggga gtgagttctt ctttaattgg ccttaagata
     3301 aatttgtcaa tatttgacgg actattccaa taacttttag gaatatctag taaatcaaaa
     3361 aatttttctt tggaaaattc agtccagcct tgagttcgat attgttttaa taatcgaaac
     3421 atagtttttg aatatgtgct atccaaatta gtaaattgct ctagattaaa tcttgtccag
     3481 ttctttaatt gattaaaaag tcctttaaat tctggattaa ctgaaattat aacaagttgt
     3541 ttatctcgta taatgctata ttttgtgaaa gcaacaaatt tagttaaagt atttccatca
     3601 tcattccaag cattaatact taataatttg tcatacgtgc tactaagatc ttttataaat
     3661 tgttccccac gttctgtata accgcttagt tttttcaaat cgtgaaatga aaaaataatc
     3721 tcatctacac ctttttcacg tacacgtgaa gcaattgaaa aaaataagtt catttcacga
     3781 ctattaaatt ttctcagagg aatatgattt aatctatttt catattttac aatttcattt
     3841 gccataagct tctcctactt ttataaagta tgaaataaaa gtaacatact ttataaaaat
     3901 actcaataat gtcggaaaac ctacttgaaa agcgtagaaa acctacttga aaagcgtaga
     3961 aaacctactt gaaaagcgta gaaaacctac ttgaaaaaac tctgaattgc ttgggagagt
     4021 aaggacacag ccgacataaa agagatataa aaagagatat aaaaagagat ataaaaagag
     4081 gggaggctaa ttttcccgcc taaaaaatca ctcctttttt ctctaaacag gttcgcgaat
     4141 ttatcaaaac tgccaattcc aaaccttgct ccctacggtt cgctaaaaag gtgtgatcgc
     4201 tctgctctca cattatactg cgcagtcgcc tccggctcct ttgctaaaag ccgttcataa
     4261 ggaacccttt gagaagctct gagcgttcgt ttaagacatt ttaagacagt cctagtataa
     4321 atcccttatc ttgttcttaa aacgccttac aggcggtcag ggcaagccct aacaacccta
     4381 aaattgtcta aatgccctct aaaattcgat ttaagcactc ttaaacctat tcttatatat
     4441 ttatatactt aggcgtttca aacgtc
//
