LOCUS       NZ_CP065694             4783 bp    DNA     circular CON 15-FEB-2024
DEFINITION  Enterobacter asburiae strain FDAARGOS_892 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP065694
VERSION     NZ_CP065694.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450422
            Assembly: GCF_016027695.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 6.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 589.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 05:35:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,713
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,501
            CDSs (with protein)               :: 4,501
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 33 of 100
            Pseudo Genes (incomplete)         :: 62 of 100
            Pseudo Genes (internal stop)      :: 18 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_892"
                     /culture_collection="DSM:17506"
                     /type_material="type strain of Enterobacter asburiae"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed2"
                     /collected_by="DSMZ"
     gene            332..>454
                     /locus_tag="I6G49_RS23730"
                     /pseudo=""
     CDS             332..>454
                     /locus_tag="I6G49_RS23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(774..1130)
                     /locus_tag="I6G49_RS23435"
                     /old_locus_tag="I6G49_23435"
     CDS             complement(774..1130)
                     /locus_tag="I6G49_RS23435"
                     /old_locus_tag="I6G49_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1333..1650)
                     /locus_tag="I6G49_RS23440"
                     /old_locus_tag="I6G49_23440"
     CDS             complement(1333..1650)
                     /locus_tag="I6G49_RS23440"
                     /old_locus_tag="I6G49_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1837..2079
                     /locus_tag="I6G49_RS23445"
                     /old_locus_tag="I6G49_23445"
     CDS             1837..2079
                     /locus_tag="I6G49_RS23445"
                     /old_locus_tag="I6G49_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2750..2965)
                     /locus_tag="I6G49_RS23450"
                     /old_locus_tag="I6G49_23450"
     CDS             complement(2750..2965)
                     /locus_tag="I6G49_RS23450"
                     /old_locus_tag="I6G49_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2972..3463)
                     /locus_tag="I6G49_RS23455"
                     /old_locus_tag="I6G49_23455"
     CDS             complement(2972..3463)
                     /locus_tag="I6G49_RS23455"
                     /old_locus_tag="I6G49_23455"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3498..4202)
                     /locus_tag="I6G49_RS23735"
                     /old_locus_tag="I6G49_23460"
                     /pseudo=""
     CDS             complement(<3498..4202)
                     /locus_tag="I6G49_RS23735"
                     /old_locus_tag="I6G49_23460"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4141..4464)
                     /locus_tag="I6G49_RS23465"
                     /old_locus_tag="I6G49_23465"
     CDS             complement(4141..4464)
                     /locus_tag="I6G49_RS23465"
                     /old_locus_tag="I6G49_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4580..4753)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          2..441
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.331"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2659..4151)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4148..4464)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 gcgagcggtg cgggcttacg aacggggcgg aaatttcctg gaagatgcca ggaagaaact
       61 taacagggaa gcgataaggc cacggcagag ccggttttcc ataggctccg cccccctgac
      121 gagcatcaca gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      241 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcggttc
      301 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
      361 ctgcgcctgt tccggtaact atcgacttga gtccaacccg gaaagacacg acaaaacgcc
      421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      481 gttcttgaag tggtggccta actacggcta cactggaagg acagtatttg gtatctgcgc
      541 tctgctgaag ccagttacca ggttaagcag ttccccaact gacttaacct tcgaaaaacc
      601 gcctccccag gcggtttttt cgtttacaga gcaagagatt acgcgtacag agcacggatc
      661 tcaagaagat cgtaagaaga aagttaacat gagcgttttt atccctcaaa aagaaggctt
      721 ctagttatag aagccttctt tttgagggat aacttatatt tttttaaaaa aaatcatgca
      781 atagcatgtt tcttctcaga actatcaaca atgtgtttcc tttctatgat ttcatgagcg
      841 ggaatcactc tgtctatttc ttcttgcaat cgagggctac tagtaacctc ccataaatca
      901 acagactgct gtatatttag ccagaacttt gcagtggtat cgaaggcttt agccaaccta
      961 aaagccatat cggtagacag ctttctatta ttgttaacta aagcactggc agagtttcta
     1021 tgcacattca acatttcagc caagtcattg atttttaaac cacttggttc gagatattca
     1081 taaagaataa tatcccctac agttgatggt tttctagttg cttgggacat gctttactcc
     1141 taaatttttt acttctctct gttagatgag gaagataagt atttcgtcta ttaccagctg
     1201 tagcgatgag taattatctt aaatatagtc acgtcatttt ttttattaat cattatttgt
     1261 aatgacattt taatgtaggt gagggaacgc ctcacctact tgttttataa gataacagcc
     1321 acgacctgtt atctatcggt aatcatgtgg atcgagatac aaatcctcga ctccgttttc
     1381 accccactta aatatcaagc ggtacttctc attcacccgt atggatgaat accctgtcaa
     1441 tggagggttg agattctcaa atcggtttcc cgggggagac cgcaaatctc caactttatc
     1501 agcagcattc agcatatcca gcttgcgacc taatgaagca ctaatcgcag ttggaatctt
     1561 tcgattggct ttaccatatt tgtaaaactg gtatagccaa tcgtctcgaa actgaatgcg
     1621 gcaatcatca tgttcatgat cttgctgcat ttttctttta cctcaatttt tgccgcaact
     1681 tggcaatttt ttcgatcacc ttcttacctc cgatatagta cagcaaacat atcaaatgca
     1741 caacagcaca gtgcacttgt gcaatcaatg gctatgctgt agaatgcact catgcacagt
     1801 gcgggtgtgc aaaataaatg ataatttgga ggttgcatgg ctcttgttcg ctcaggaaac
     1861 aaaagcaaga tttttgaagc agatacgaag tttaactcgg tttttgacac tgaatataag
     1921 ccgggagaaa catgtgaata tgaagggatt tataaatgta ccggttgcgg taaagaagta
     1981 actatcgcag gcgggaaaac aatcccacca caaaaccacc accaacatac ccccgcgcaa
     2041 gggaacattc gttggcaatt gtttgtttat gctcagtgag gtatagaaaa tggcagatcg
     2101 tcgaccactc aacccacacg ttgtcgttcc taaaccaggc ggcggaactc aacctcgtga
     2161 ccgtaacaat gacggaaggg tcagaggaaa acgctcagat gcaggaaagc cacgaaaata
     2221 acagcaagcc gccagcctgg cggctttttt atatctgcag ctgatactga tttaccataa
     2281 catccgttgg gcgcaccgct gaaacggggt tggctgcgtt tccggtgcca gacgcgagaa
     2341 agtggcgaca gacataacga aaaaacacca gaaacgcagc cgctgaatat tacactggtg
     2401 catagtcatg cagccagatg catggatgtt aaagtcacac taaaatccgc gccaatgcct
     2461 taaaaaccga caaatgcttc tccagccagt cccacagctc cgccgccaga tacaggcagt
     2521 taagcaatga ttcaatgaga tttaacagct tcatttcccg ctcctgaata agtcgtgacc
     2581 tttttgatca cgaacctatt tagcgggaga aataagagcc cctcttcgtg cgggttgtca
     2641 gctgcctgga tggtttaaag tgtcggcccg cggtaggttt tctgcggctg gcgctccagc
     2701 tccagtgttt tctgatactc attctccctc tgccggagct cgatggcatt cagttcccgg
     2761 acaagcggcg cagctgctcg cttaaactca gcacctgcgt gctcaagtcc gacacgcgct
     2821 cgttcagcac cgcgttctcc cgttgcataa gcccacacat gctccgccat tccgcgaagg
     2881 cgctctccca ttcgtccagc ctttgcgagt agtcctgctg tagctgctct aatgcgctca
     2941 gcaactgctt ttccagctct gtcattttcc gctactcctg tttcaggcgg atccatgtcg
     3001 tcccgtcgta ggggatggct tcggtttgca tcccgggcgg caggataaga aacctccggc
     3061 cgtcgctgct ctcctgatag cgcactcccc acgtccgggc agtcattttc gccagggtat
     3121 cttcctgctg acgaagatgc gtgtaattgt cggttatctg ctgcccctgc caccacagta
     3181 tgctcccgct cgtgccgatc agcagcgctg acaccatcag gatggtgaac catgtccgcc
     3241 cggcagtccg cagtacgctc cgccggtttt tctccatcgc ctcgctcata ctctgctcgt
     3301 gtgcgaggat ggcgtcgctg attctcctcg cgcttgcgct cagttccgct ctgacagact
     3361 gctcgtgctc gctgaatgcg gctttcagca tctcgccggt actctgctgc tgcgctttcg
     3421 attgctgctc taagtccttc gccagcgtta aaagactgct catagatggc tcccttcagt
     3481 cggatgtttc gccccccgtc cgggtcggca atgctgatac tgcttttcgt ggtgcgcacc
     3541 acctcaaaac ctgaccgttc cagcgcctca gtgacgtcct gacggttttt cagctccccg
     3601 gacgaagcaa gggtcagcag tccgcgcgtg atggcttcag tggcctcctg cttcgttttc
     3661 ggcagtccgg acggcgtgac cagcgcccgg cggttttccg gcgcgttcgg gtcatgcagc
     3721 cccagtctgc cgttcacgat ggtctgccag gcatcgatgc gcggccggtc ggcgcggtcg
     3781 tagtacggct ggagccgcct gccggtcagc agttcggtat tggggatcag aaagttcagc
     3841 tccagacgcc ccttgtccgc gtgctccacc cacagaatgc tgtactgatc tttatcgagt
     3901 ccgggcatca gaacccgctc gaagctggcc atcagttttt cccgctggcc gggaggcaga
     3961 tcggcctccg caaacgacag tacgccggag gtgtatttct tcacgtaagg cgacgcgtca
     4021 atcagctccc ggacttcttc cggcttcccc tgcaggacgc tggcgccttc acgctgccgg
     4081 tctttaccca gcagatagtc caccggcccg gcgccaccgc cgcgaccgcg tggatgaaat
     4141 ttaacgatca tcgtccgttc ctttctcaag aacggcgtgc cgcagccgct caagcccggc
     4201 atcgatggcc atcagcgccg ccacaatctg taccctgtca tggccggtgc cgccgccggc
     4261 gttaacacgg cgggctatct ggttcaggtt attgcccata ccggcaagct ggcgcagcag
     4321 cgccggcgag agtgagggga gctttccggt gcgcgcaggc ttttcgtcca ggcaggtctg
     4381 ccgcatccac gccgccagcg gcctgccgtc gcagcgctca agcagccgcc ggtgctcgcc
     4441 ctccgtcacc cacatggtga gcatcttgtt ccgtttctct gacaaaccgg cctcctgata
     4501 acgccgggac gggcgggaaa gcctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
     4561 cgtcagtggc ttttggcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
     4621 gagtgtatac tggcttaata tgttggcacg gatacggaaa tcagtgaagt gcttcatgtg
     4681 gcaggagaaa aaaggcggca acggcgcgtc agcagaatat gcgatacagg atatattccg
     4741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcg
//
