LOCUS       NZ_CP065658            22021 bp    DNA     linear   CON 15-FEB-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_913 plasmid unnamed4.
ACCESSION   NZ_CP065658
VERSION     NZ_CP065658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450443
            Assembly: GCF_016026915.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22021)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22021)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7
            Genome Coverage                   :: 2034.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 04:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,517
            CDSs (total)                      :: 2,433
            Genes (coding)                    :: 2,341
            CDSs (with protein)               :: 2,341
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 58 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 33 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065658.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..22021
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_913"
                     /culture_collection="ATCC:12228-1"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed4"
                     /collected_by="NIST<ATCC"
     gene            331..1260
                     /locus_tag="I6G70_RS11940"
                     /old_locus_tag="I6G70_11940"
     CDS             331..1260
                     /locus_tag="I6G70_RS11940"
                     /old_locus_tag="I6G70_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_077431479.1"
                     /translation="MNKEEYLKIIQKETKKINTDEKEDILSKYESYFINGYKNNKNDEE
                     IIKELGDPVKIAKEINAVNSLYKVENKKSVKSIFSAAFSIMELSILNLILIISIFYTMT
                     QVHNNHKVSGETILTLSTWLFAFWGVMIITSFLVRDFSQGTSQLYLNSSKNRVKYFISQ
                     LISILIASVIVFLTLYVFVLIMQNIGDGEPLKNEVVGKALGIYILLFLFYGLFLFLITL
                     LVKSSSLVFSIGVFLILIIPIATNLVPLIPEYGDEVRKALKYIPFNFLINNVWLGSLKL
                     NGWQTFISIASVILLAIIDFFTISKRDY"
                     /name="DUF1700 domain-containing protein"
     gene            complement(<1494..1629)
                     /locus_tag="I6G70_RS12665"
                     /pseudo=""
     CDS             complement(<1494..1629)
                     /locus_tag="I6G70_RS12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829553.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /name="RepB family plasmid replication initiator protein"
     gene            <2277..2600
                     /locus_tag="I6G70_RS11945"
                     /old_locus_tag="I6G70_11945"
                     /pseudo=""
     CDS             <2277..2600
                     /locus_tag="I6G70_RS11945"
                     /old_locus_tag="I6G70_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3107..3199)
                     /locus_tag="I6G70_RS11950"
                     /old_locus_tag="I6G70_11950"
     CDS             complement(3107..3199)
                     /locus_tag="I6G70_RS11950"
                     /old_locus_tag="I6G70_11950"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_115172131.1"
                     /translation="MSLIFVCIIAPIIVECCVAIFTFWLNNRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3652..4071)
                     /locus_tag="I6G70_RS11955"
                     /old_locus_tag="I6G70_11955"
     CDS             complement(3652..4071)
                     /locus_tag="I6G70_RS11955"
                     /old_locus_tag="I6G70_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002502136.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICALADIREMNVTEYIRHTAIG
                     NRIKPTVIEQIKESSEVSELFKKDNEALKSNNQALQHHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLNFNTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            4526..5734
                     /gene="mobV"
                     /locus_tag="I6G70_RS11960"
                     /old_locus_tag="I6G70_11960"
     CDS             4526..5734
                     /gene="mobV"
                     /locus_tag="I6G70_RS11960"
                     /old_locus_tag="I6G70_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_071965886.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLI
                     EYERHLYHRVKDRVDYYKEQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     HLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRTISRDDFKKDEEKYQKEIKAYEASL
                     SGLRMNYRDLNKRNQHLTTQNNQLEHHLDLLNARHQAMNDMVRNDTRILFAGLRCALKD
                     RNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            complement(6493..6648)
                     /locus_tag="I6G70_RS11965"
                     /old_locus_tag="I6G70_11965"
     CDS             complement(6493..6648)
                     /locus_tag="I6G70_RS11965"
                     /old_locus_tag="I6G70_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="WP_018113591.1"
                     /translation="MEHISKLGEAIVNTISASQSGDSAELAKSIVNIAANGIGVISDVA
                     KAFGLM"
                     /name="beta-class phenol-soluble modulin"
     gene            7156..8022
                     /locus_tag="I6G70_RS11970"
                     /old_locus_tag="I6G70_11970"
     CDS             7156..8022
                     /locus_tag="I6G70_RS11970"
                     /old_locus_tag="I6G70_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494364.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_018113587.1"
                     /translation="MTGETVVYKNDLNLVPLRKFTSTEINLFFAMCNKLKEKETNTLHL
                     SFDELKELSNYSPKTRNINRFVKDLDDVYKKMLELTIRYEDDEVIERFILFNHYRIHKK
                     DQYLEISTSPNLKHILNSITSNFTKFELKEMTNLKSTYSKNMFRILKQYKHTGYMKIKI
                     DDFRERLDIPKSYRMTDINKNVLKPIVTELNAIFNNLHINKIKAKKGRKIEWLEFIFDP
                     EKRIHNKRQPQMTNVGKQRQYINREKTPEWLNEGRLGQSQEIHNEDSKLRQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            8690..9256
                     /gene="lepB"
                     /locus_tag="I6G70_RS11980"
                     /old_locus_tag="I6G70_11980"
     CDS             8690..9256
                     /gene="lepB"
                     /locus_tag="I6G70_RS11980"
                     /old_locus_tag="I6G70_11980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503824.1"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA];
                     GO:0006465 - signal peptide processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="WP_018113588.1"
                     /translation="MKKEFKEWLIAIIVAILLLFLVNNFLFKPYTVSGLSMYPTFNNKD
                     KVVVSKISKSLNHLKSGDVIIFHQNSNNDFIKRLIGKPGDQIEYKNDKLYINKNYIKEP
                     YLDYNKKMNNSSENLTEDFNVSNIKGSKHKMIIPKDKYLVLGDNRTNSIDSRYSEVGLI
                     SKKQIVGKVILRFWPFNDMKYNFKR"
                     /name="lepB"
     gene            complement(9457..9555)
                     /locus_tag="I6G70_RS11985"
                     /old_locus_tag="I6G70_11985"
     CDS             complement(9457..9555)
                     /locus_tag="I6G70_RS11985"
                     /old_locus_tag="I6G70_11985"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_309498286.1"
                     /translation="MMYLIFVFIIAPIIVECSVTLFKYWLEQRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(9863..10348)
                     /locus_tag="I6G70_RS11990"
                     /old_locus_tag="I6G70_11990"
     CDS             complement(9863..10348)
                     /locus_tag="I6G70_RS11990"
                     /old_locus_tag="I6G70_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020368224.1"
                     /translation="MKKFSKIVLASGIVLGFSVTPELFNATQAQAQESIEPHYNYDGYT
                     AYQSGFILDKNFKESLKYDNFTINGYQISKNPTGDNRINLYDQTLYGVSENKANGVFFS
                     LDGKSINKNTLIQNYGQPNSYSPTSAWGEEYMYKIGNKDVRFYIYNGYVIKCQIISE"
                     /name="hypothetical protein"
     gene            complement(10864..11229)
                     /locus_tag="I6G70_RS11995"
                     /old_locus_tag="I6G70_11995"
     CDS             complement(10864..11229)
                     /locus_tag="I6G70_RS11995"
                     /old_locus_tag="I6G70_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494365.1"
                     /translation="MKWLKIIILILSLVPIEFIGLFTDYQTGLLIGYIPFIIVAILISR
                     SIFKFGLKNNISIIISRCIGTFLSWECVHWFMNIYDSSDYFKPLTTDIFALFLGAIHFI
                     VIMLIYLVIYGFSHRNN"
                     /name="hypothetical protein"
     gene            complement(11359..11727)
                     /locus_tag="I6G70_RS12000"
                     /old_locus_tag="I6G70_12000"
     CDS             complement(11359..11727)
                     /locus_tag="I6G70_RS12000"
                     /old_locus_tag="I6G70_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494366.1"
                     /translation="MKKILKILITSIIVIIITLTVWTFSVITYQEHKSEKIIDHVIERK
                     GWDKKIKNEKMSFNIMMGYAEKDIAFKDQPYSEYEYNVTPAPAPWTDDKEYKVWGETDL
                     QKKDSYYKYLLESEPYRK"
                     /name="DUF3139 domain-containing protein"
     gene            11831..12226
                     /locus_tag="I6G70_RS12005"
                     /old_locus_tag="I6G70_12005"
     CDS             11831..12226
                     /locus_tag="I6G70_RS12005"
                     /old_locus_tag="I6G70_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494367.1"
                     /translation="MKRKYKITGIIFIVLLIVLTLIFSIVHHYANVQKHEEAKLKQKVH
                     HIFKQKGWEDKVKEEKNIFTFNTGDNDLQVTFKDEPYNTYTYSIDEDNKVYGNAVLKDE
                     YDKDFDSKKKYKEYLRKMHFEEKYDLK"
                     /name="DUF3139 domain-containing protein"
     gene            12460..12639
                     /locus_tag="I6G70_RS12830"
     CDS             12460..12639
                     /locus_tag="I6G70_RS12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001829546.1"
                     /translation="MKSIVHFICLFIIFCIFGYVIDLIFFKEINIFLILESAFILSLLI
                     MPILKIVKEIEIEK"
                     /name="hypothetical protein"
     gene            12837..13286
                     /locus_tag="I6G70_RS12010"
                     /old_locus_tag="I6G70_12010"
     CDS             12837..13286
                     /locus_tag="I6G70_RS12010"
                     /old_locus_tag="I6G70_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494368.1"
                     /translation="MKKLLSVFIASLLVLTGLSFSSINQGNTASAKTKYKTTITYKGQK
                     YVYVGHYKHHFSKKEVKKVVKFSKGVHSGNKLVSAASKLSKNGYVKASSAIYKAFDFGL
                     KNELKGSYFYTAAKKGTGVTISYDMYTATTGPNHTIAKNFKHSYN"
                     /name="hypothetical protein"
     gene            complement(13446..13832)
                     /locus_tag="I6G70_RS12015"
                     /old_locus_tag="I6G70_12015"
     CDS             complement(13446..13832)
                     /locus_tag="I6G70_RS12015"
                     /old_locus_tag="I6G70_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494369.1"
                     /translation="MKKAIFSIIISLILVLTVTGCSNSSKEKPIKKSALEINPTSKAVN
                     ITINKKENNKPEKIGKVYRYKNNNAKEITNDGIKKDTKDTLIWKGVANKYDNVKDLLGE
                     SILYEVKYKNGDIKKFERKIKYTE"
                     /name="hypothetical protein"
     gene            complement(13852..14013)
                     /locus_tag="I6G70_RS12020"
                     /old_locus_tag="I6G70_12020"
     CDS             complement(13852..14013)
                     /locus_tag="I6G70_RS12020"
                     /old_locus_tag="I6G70_12020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162153024.1"
                     /translation="MNEREQRQKLNITLPPKLITNLDKVKTKYGINKSKQIENLISKYL
                     NEEYGNLE"
                     /name="hypothetical protein"
     gene            complement(14806..15324)
                     /locus_tag="I6G70_RS12025"
                     /old_locus_tag="I6G70_12025"
     CDS             complement(14806..15324)
                     /locus_tag="I6G70_RS12025"
                     /old_locus_tag="I6G70_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_023567445.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGVTVHHS
                     TVYRWVQEYAPILYQIWKQKHKKAYYKWHVDETYIKIKGKWCYLYRAIDADGHTLDIWL
                     RKQRDNHAAYAFIKRLIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRSLQIYGFSPC
                     REISIMLAS"
                     /name="IS6 family transposase"
     gene            complement(15444..16355)
                     /locus_tag="I6G70_RS12030"
                     /old_locus_tag="I6G70_12030"
     CDS             complement(15444..16355)
                     /locus_tag="I6G70_RS12030"
                     /old_locus_tag="I6G70_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; predicted Zn-dependent protease with
                     a strongly conserved HExxH motif; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="WP_020368409.1"
                     /translation="MYKINIVRSDKVYLGLLDTEYENKSKYFKDNLLKYFKEKFEIQNI
                     PFESDNFRFDALQFLNMTHISPEAFNTNYRSEVALLNDAFWNDCKLYIEDGIEQFEKNG
                     IESHIDKYTFTVLLGDSKKPTMYLNNNYGGDGGIPGYIFISLVPNQYTISRIKSAIAHE
                     INHNIRYQYIKWDGGSLTELIISEGLAENFVEKMYGTKYLGPWVTEIDWDQQNNKIKNT
                     IKENLDINNMFEAMPYLYGDEITRMQGGVPVGLPHAAGYTCGYYLIKYYLEKTQRTIEE
                     ATIKSSDEILKEVNDFWNTNII"
                     /name="DUF2268 domain-containing putative Zn-dependent
                     protease"
     gene            16442..16960
                     /locus_tag="I6G70_RS12035"
                     /old_locus_tag="I6G70_12035"
     CDS             16442..16960
                     /locus_tag="I6G70_RS12035"
                     /old_locus_tag="I6G70_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_023567445.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGVTVHHS
                     TVYRWVQEYAPILYQIWKQKHKKAYYKWHVDETYIKIKGKWCYLYRAIDADGHTLDIWL
                     RKQRDNHAAYAFIKRLIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRSLQIYGFSPC
                     REISIMLAS"
                     /name="IS6 family transposase"
     gene            17075..17449
                     /locus_tag="I6G70_RS12040"
                     /old_locus_tag="I6G70_12040"
     CDS             17075..17449
                     /locus_tag="I6G70_RS12040"
                     /old_locus_tag="I6G70_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494371.1"
                     /translation="MKKIVYILGTIIVLGFCYFYVNLIFFDSWTRYSAEKEINNYISNH
                     DTKKLKEISANNKTYQLLKHAKHVTIKGDTDNQGGGHIGYFTSTINGKEGALFVHLNFG
                     LFPEIPKVTKLEVFDKNIYE"
                     /name="hypothetical protein"
     gene            complement(18040..18492)
                     /locus_tag="I6G70_RS12045"
                     /old_locus_tag="I6G70_12045"
     CDS             complement(18040..18492)
                     /locus_tag="I6G70_RS12045"
                     /old_locus_tag="I6G70_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494373.1"
                     /translation="MPKKQSSKEKILTVRVSQAQYKKIQDLAYIRSMNVTEYIRQTAVG
                     NRIKPTVIEYPKEQITMDDSNSKNNTNNLYKVIEHLEEDNEALKSDRDALRQRHDQLED
                     DIEQYKRENEIFHRLLEHFDSTAFMNFNTYRDDRPLKKEIKRLKEH"
                     /name="hypothetical protein"
     gene            18947..20154
                     /gene="mobV"
                     /locus_tag="I6G70_RS12050"
                     /old_locus_tag="I6G70_12050"
                     /pseudo=""
     CDS             18947..20154
                     /gene="mobV"
                     /locus_tag="I6G70_RS12050"
                     /old_locus_tag="I6G70_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            20827..21693
                     /locus_tag="I6G70_RS12055"
                     /old_locus_tag="I6G70_12055"
     CDS             20827..21693
                     /locus_tag="I6G70_RS12055"
                     /old_locus_tag="I6G70_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_077431477.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYNPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     ERYLEIATSPDLKYILNSITNNFTKFELKEMTHLKSTYAKNMFRMLKQYKHSGYMKIRV
                     EDFRERLDIPNSYRMSNINQFVLTPIIRELSTIFNNLKINKIKAKKGRKIEWLEFIFDA
                     EKRIHNKRQPQMTNVGKQRQYINREKTPEWLNEGRLGQFQEIHNEDTKLKQDREAFQRQ
                     LEEKWED"
                     /name="RepB family plasmid replication initiator protein"
     Typing          7157..8022
                     /name="rep_cluster_1439"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.885"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          20828..21585
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.151"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          4455..5731
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.122"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18923..20151
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.878"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaagaagtt acacgatata ctgtaattaa caaattgtag gtgtacatca ctgtacgcaa
       61 agacccccac gtagcttgcc ggctgtgggg gtctttttta ctatcgttgt ttcaaactat
      121 cataaaaata gtttgccata atactaatga tgagtggatc aacgatattt aagataacaa
      181 attgtaggat atccaacact atacgcacac ctcctttctg cacaggaagt gcgttctaat
      241 tataatatac aagttttttc aatgcattac ttaaagagtg gaatgccttt actaaaacaa
      301 taaatagatt tgtaaaagag agtggcatta gtgaataaag aagaatattt aaagattatt
      361 caaaaagaaa caaaaaagat aaatactgat gaaaaagaag atattttatc taaatacgag
      421 tcttatttta ttaatggata taaaaacaat aaaaatgatg aggaaattat taaagaactt
      481 ggtgatcctg taaaaatagc caaagaaata aatgctgtaa attcattata taaagtagaa
      541 aataaaaaaa gtgttaaaag tatattttct gcagcctttt ctattatgga gcttagtatt
      601 ttaaacttaa ttttaataat ctccatattt tatacaatga ctcaggttca taataatcat
      661 aaagtttctg gagaaactat tttaactctt tcaacatggc tatttgcatt ttggggagtt
      721 atgattatta ctagtttttt agtcagagat ttttctcaag gtacgtcaca gttgtatttg
      781 aatagttcta aaaatagagt gaagtacttt atatctcaac tgatatccat attaattgct
      841 agtgtcatag tatttttaac attatacgta ttcgttttga taatgcagaa cataggagac
      901 ggtgaacctt taaaaaatga agtagtggga aaagctttag gaatatatat cctattattt
      961 ttattttatg gtctatttct atttctaatt actttattag taaaaagttc ttctttagta
     1021 ttttcaattg gggtttttct tatattaatc atacctatag caactaattt agttccatta
     1081 attccagaat atggtgatga agttagaaaa gccttgaaat atataccttt taacttttta
     1141 atcaataatg tttggcttgg atctctcaaa ttaaatggat ggcaaacatt tatttcaata
     1201 gcttcagtaa tactattagc tataattgac ttcttcacca tttctaaaag agattattaa
     1261 agattaacaa aaccaatgtt tttcctaaca gactgattaa gagcgattag catagttgct
     1321 agtcgctcat tttattgttg ttctttcaat cgcttaatcg cattttttaa ggatctttat
     1381 tattaagact aaaccataat atatattatt atgaatttta aaatgatata acgctcttat
     1441 aaaagatgct attacaataa aattgtatga ttatcaataa aaaaacgatg tctaaacgga
     1501 ataattactt ttctcgtatc ctgttcttta agtttattgt ataatggaaa gaataaatct
     1561 acttcagtac ttgtaaaacg tcttaatggt actaaactta tttatatata ctactgtttt
     1621 accagacata aaaaactcct ctccaaatat ataggtgatg caagatacaa aacatctctt
     1681 ttaacgtctc aaacctcttt ttgctttaaa gctgagatag tgtctaaaaa atttataaaa
     1741 agatattaaa aatttttggg ctttagccct tcacaaattc aaaaaaacaa aaaggaaaga
     1801 gtccagagat tttatgttcc ggactcttca attctaaaat atttagttta gtaaagaata
     1861 tagttactag taattgtact aggttataag ttaatgttaa atagtttcgg tatcaataat
     1921 ttttattaaa gaccaatacg ttttaagaat ccaataaatt cttcatagat atcttctaac
     1981 ataaatttcc ctccttgata ttacaaataa gtaatatact tagcaataat tattttgtgt
     2041 aaggtttgtc ttggccataa tattcgattt tatcatagaa gaattgacca aaaattgtta
     2101 agaagcgatg taacatgtaa tggcacctcc ctatttacta taatacatat ttagtataac
     2161 cctattttca aatcataaaa catttattta caattataag tgaaatgttt cttaattaag
     2221 tttaaaaatg aacagaataa cgctttttgt aatagataat attttcgctt aataagagta
     2281 tacctattat gaacgaagtt attggtaacc cattattaga taaatttatg aaaaatctaa
     2341 tcattcaaat attagcaatg gtttcagaac aagaaagaaa tgaaagtaag cgcagacaag
     2401 cacaaggcat taaagctgtt aaagaaaaag gtgtatataa aggacgccca ttattgtatt
     2461 ctgcagatgc aaaagatcca caaaaaccca ttatctatca tcgagttgta gaaatgttag
     2521 aacaagtcaa tacaattggt aaagaagtca atatcacaag acacacggtt cataggatta
     2581 aacagaataa aaacatataa atgtgttcta tttcaatcaa agagaaaaag tgagtgacaa
     2641 tctagtatat acagaagttt tcctacacgc tgataagata gtatagagga tgcactctat
     2701 tctttgatct agttgataac tataatagag aaaattggaa aagtataatt ggaagtaaag
     2761 ttattgataa tcatataaaa cactaaattg acttctcata atcacagaca aataataaga
     2821 atttggcttt aatatgttta aagtttttta ataaggttaa acaaagtatg tgagacaaaa
     2881 aataaggaag tataatttta agcgatgaat gataaaaaag ctaagttata aagctagctt
     2941 gagatattgt tgacgattat ataattagtt cattcgaata tctatttaat tttacaaatc
     3001 tcttcatgca agatatactg aaattaacaa atattgaact catgtatcac cgaaccccaa
     3061 cctatgccag taggttgggg ttctttagtg gtgaggctat gtcgccttat ttattgcggt
     3121 tgtttagcca aaacgtgaaa atcgcaacac agcactctac tataataggt gctattatac
     3181 aaacaaatat taaactcatg atttcacctc cttccgccga atctatcgcc ggaagtaaga
     3241 cgacctaagt attataacag aaacttaatt aatatacttc tttggtatta ttctttatat
     3301 aacaaataaa ataaatgaaa aatttcagat aactaatcaa ataaataaat ttaaaccgct
     3361 gttttgtcat ttgagatttt gaataaagat acaaaatgct cacaaatgaa aaagaatgaa
     3421 aacgcgtcgt taaagggatg tataaaagtt gtataaaggg aaatgatgac aaatagggac
     3481 aaatgccgaa atgacggcgt ttgtcccttg attgtaagtt aacataatta actattatag
     3541 gtagttagtt tgtatagttt atgcatcttc taactagtta tcggtgacta ttacatcata
     3601 agttagaagc tacttaatat aataaaagca agtgattcac aaaattgtaa atcacttgct
     3661 tttatactgt tcttttaatc gtttaatcgc attttttagt ggccgatcat cgcgatatgt
     3721 attgaagttt aaaaatgctg tactatcaaa atgttgtaat aagtgatgaa agacgtcatt
     3781 ttcttgtctc agttggtcga tttcatcttc caactgatga atatgatgct ggagtgcctg
     3841 attattcgat ttgagtgcct cgttgtcttt tttaaacaat tcactgactt cactggattc
     3901 tttaatttgt tcgatgactg tcggtttaat gcgattacca atggctgtat gacgtatgta
     3961 ttcggtcaca ttcatctctc gaatatccgc aagtgcacaa atcttttcgt attgcgattg
     4021 acttacacga attgttaaga ttttttcttt tgaattttgt ttttttggca ttgaatcacc
     4081 tcataattaa tatatgataa gcatacatat gtgtgcttat ctttatatta atataacata
     4141 catatgtatg ttaataaata tgtatgattc gaggtgttgt gagctaacat ttggcataca
     4201 tattatcaaa catatttgtt tgataatgcg tatgtcattt gttagctata gaacggaatt
     4261 caaaaatata tttttgaatg tagtgatggc ctcgccagag cgaagataac tcttacattt
     4321 gaatgtaaga gtatatcgag ctatactctc attgcggaat cagcctcaca tgtttgaggc
     4381 gtagcggtga gagtatccat cattttacat aagcgaagca ttgtcgaagg tagacaatca
     4441 cagcgcttgt aaaatgatat ttgcgaaggt tctcgaaagt agagaaccac agacaaatca
     4501 aaaagcttcg ggaagggtgc tcaatttgaa attagcttgg aatacgcaaa aaaataaaat
     4561 gagcgatgtt aaaggcaaag agatggaaca agaacgtaaa gggagaatca aaaacgatga
     4621 gattgatttg aaacggacaa acttaaactt tgatttaatt gaatatgaac gtcatttgta
     4681 tcatcgtgtg aaagatagag tcgattatta taaagaacag gggtcacgtg tacagaaaaa
     4741 tagtgtggtg atgtattcca atatcattac gttatcgaaa gaagaggccg accgtatggg
     4801 agaaacacgt acaaaacact actttaaaac atgtaaagat tacttcagtg aacgttttgg
     4861 cgaagccaat gtcgtttcag cgaaagtgca tatggatgaa tcagcgcctc atatgcacct
     4921 tcactttata cctgtgaatc atcaagggcg gttatcagca cgtacagcta tgaatcgaca
     4981 agccattaat cacatccatg atgaactaac aacacatctg tgccagcaag gttttgatgt
     5041 tgaacgtggg agtaccgatg ataataaaac gtatattcaa gatattcatg aatacaaaaa
     5101 gaaagcgaaa gttttaagtg aattggacca acaaattgaa acgaaaaaac aattgttgag
     5161 ggatatcaac gatttacacg atgaaaaaga acatttaact cgggatattc aaaacttaaa
     5221 acggaaaaat cattttttac aagaggattc tcaacaggct caagaagata tagacctata
     5281 caagcaactg agaacgattt ctagggacga ttttaaaaaa gatgaggaaa agtaccaaaa
     5341 agaaatcaaa gcgtatgagg cgtctctgag cggccttaga atgaattacc gagatttgaa
     5401 caagcgcaat caacatttaa caacacaaaa taatcaactc gaacaccatt tagacctgtt
     5461 aaatgcacgt catcaagcga tgaacgacat ggtaagaaat gatacacgta ttttgtttgc
     5521 gggacttaga tgtgcattga aagataggaa cattgaatta agcgcagaaa ttaaatatca
     5581 acgtatgcca gaagtcggta ttgatcccga tacggtggat cagggggctt taacacatgc
     5641 atatcaaggt gatgtgtcag ggatagacca tcatataaga aaggcatcag cacctattaa
     5701 aaaagagaca gagaatgact tagaattaga ataagtcaat aaaaataagc actcaggcaa
     5761 gtatgatact tgtttgagcg cttatctcaa tgaaaagagt aagtaaaaac ttaataagct
     5821 ggcgacactt attaagagca ataataactt taattactga attagtagac tagttcagta
     5881 attaggaacg atacctttgt atttctatat tattataaga attccaaggt agcaatgata
     5941 ttgatttaat atgagcaaat tgatattgga agcaaaagaa acccactcaa acgaacgagt
     6001 gggttttgaa aaatgatacg tattaacttg ataaaattaa tcattttata gtttaaagaa
     6061 aaggaatgca taattatagt aacacaaatt aatgtttgtt acactagtag tgctgctcaa
     6121 tatataaagt agtaaaccat aactattctt cgcatctctt gttaaaaagt taattttttg
     6181 ctttgtacaa ggaaaagcac tcaaaattga gtgctttttt catattaaaa ttaaacttta
     6241 aaaattaaga cagcgacaca tatcagtgtc gctgtcttaa gatatattat atgagttcaa
     6301 aagatatata gctattgtag cataatagtg tttttattaa aaaacaagac gaaatagttt
     6361 gaattactaa ataacttatg tcttaaatct tttaatttat ttaagatacg ttgttattaa
     6421 gaaaataaaa aaagatataa atcaactaaa taattgattt atatcttcat atgtattgat
     6481 ttaaaatact tactacatta aaccaaacgc tttagcaacg tctgatatta caccaatacc
     6541 attagctgca atattaacaa ttgatttagc taattcggca ctatctccag attgtgatgc
     6601 tgaaatagta ttaacaatag cttcacctaa ttttgaaata tgttccatga aaaacaaacc
     6661 tccttacata taaaatcatt actcaaataa aataacatat attacgataa ttctcttcta
     6721 cttttaaata attgtaatta aatttacgtt ttcttaaaaa taaataaagg gattgcaatg
     6781 ttgttactat taaaattaat aaaaaaactt tgaatataat aatagctatt ttacatggaa
     6841 ttttttaata tctttttata aatcttttta taaacctttt tagaagtcgt ttgagcttta
     6901 gagccacagc ttatttgaga tgcttaagga gtagttttgc cacataaaag gagtagtttt
     6961 gccacataaa aggagtagtt ttgccacata aaaggagtag ttttgccaca taaaaggagt
     7021 agtttttatt ataaaccttc ttttgttaat tgacattgtt atcaaagtgt tttaacgtga
     7081 aaatatcaat tcaataagga gtatcataaa aaaatatgct ccttattgaa ataattatgt
     7141 tgaggtatct atataatgac gggagaaaca gtagtataca aaaacgattt gaatttagtt
     7201 cctttaagaa aattcaccag tactgaaatt aatttatttt ttgccatgtg taataaactt
     7261 aaggaaaaag aaactaacac acttcattta tcttttgatg aattgaaaga attgagtaac
     7321 tatagtccga aaacacgtaa tattaataga ttcgttaaag acttggatga tgtatataag
     7381 aaaatgttgg aactaacaat tagatacgaa gatgatgagg ttatagaaag atttatttta
     7441 tttaatcatt atagaattca taaaaaagat caatacttag aaatttcaac ttctcctaat
     7501 ttaaagcata tacttaattc aattactagc aactttacta aatttgaatt aaaagaaatg
     7561 acaaatttga aatctacata ttcaaaaaac atgtttagaa tacttaaaca atataaacac
     7621 actggttata tgaaaattaa aattgatgat tttagagaac gcctagatat ccctaaatct
     7681 tatagaatga cagatattaa taaaaatgtt ttgaagccta ttgttacaga gttaaacgct
     7741 atttttaaca atcttcacat taataaaatc aaggctaaaa aaggacgtaa aattgaatgg
     7801 ctggagttta tctttgatcc tgagaaacgc attcacaata aacgacagcc tcaaatgacc
     7861 aatgtaggta aacagcgtca atatatcaat cgtgagaaaa caccagaatg gttaaatgaa
     7921 ggaagattag gacaatctca agaaatacat aatgaagatt caaaattaag acaagatcga
     7981 gaggcatttc aacgtcaatt agaagaaaaa tgggaggaat aataacttaa atgagctaca
     8041 tctatatttt gcttttattt cctttaataa tgggattttt cttatcacta aaagagaaaa
     8101 agtatgataa aaaatgacta attcactatt gtaaagaaaa tacagaattt tatgagtaaa
     8161 acttgtaatt ttattgcaag tgaagaaact acagtatata ctgaaactaa taaatattga
     8221 actcatatat caccgaaccc caacctactg acataggttg ggttctttag tggtaaggct
     8281 atcttgactt atttattgcg gttgtttagc caaaacatga aaatcgcaac accgcatcct
     8341 actataattt gaataaagca ttaaaaatgg ccactcaaca atttgagtgg cattgaaatc
     8401 gttaaggagt tacttacgtt aaaaaaatta atgttagtta gggacaatta tatgttttca
     8461 ctttaacact tatttatatt taactcactc aaaaaatatt taacgctgga aatttttgcc
     8521 ttttttgaaa cgagtggaac gagttcattc tgttcttgat agtaaatttt attatttcaa
     8581 taaagaaaag acatcataaa taatgaacta cttttggtag gatattaaaa atataagtga
     8641 taacctaaaa taggataaag tttataaaat ctaaaaagga aattatgtaa tgaaaaaaga
     8701 atttaaggaa tggttaatag ccataatagt agctatatta ttattatttt tagtaaataa
     8761 ttttttattt aaaccttata ctgtatcagg attatcaatg tatccaacgt ttaacaataa
     8821 agataaagta gtagtcagca aaatatccaa atcattaaat cacttaaagt ctggagatgt
     8881 tattatattt catcaaaata gcaataatga ctttataaaa cgattgattg gtaaaccagg
     8941 agatcaaatt gaatataaaa atgacaagtt atatatcaat aaaaattata tcaaagaacc
     9001 ttaccttgac tataataaaa aaatgaataa ctccagtgaa aatttaacag aagattttaa
     9061 tgtatcaaat ataaagggat ctaaacataa aatgataata cctaaagata agtatttagt
     9121 tctcggagac aataggacaa acagtattga tagtagatat tctgaggtgg ggttaatatc
     9181 taaaaaacaa atcgtaggaa aagttatttt aagattttgg ccttttaatg atatgaagta
     9241 taattttaag cgatgaatga taaaaaagat aagttataaa gctagcttga gatattgttg
     9301 acgattacat aattagttcg ttcgaatatc tatttaattt tacaaatcta tacttacaag
     9361 atatactgaa agtaacaaat attaagtgca tcaaaacacc aatcccccaa cctatgccag
     9421 tagattgggg gatcttttag tgttgactat gtcgtcttat tttttattac gttgttccag
     9481 ccaatattta aacaacgtaa cactgcactc tactataata ggtgctatta taaaaacaaa
     9541 tattaagtac atcattacac ctccttccgt cgaatctatc gccggaagta agacgacttc
     9601 aatagtataa caaaaatttt taatttaaaa cgatagttta ggacttgcca tatgaataaa
     9661 gatacaaaag ttcacttgat gaaaaagaat gaaaacgcgt cgttaaaggg atgtataaaa
     9721 gttgtataaa gggaaatgat gacaaatagg gacaaacgct gaaatgacgg cgtttgtccc
     9781 taatatgtta gttaacataa tataaagaaa acatgaagta agcaaacttg caacttacct
     9841 catgttttat acctaaaatg tcttattcac ttataatttg acatttaatc acatagccat
     9901 tgtaaatata aaatctgaca tctttattac caattttgta catatattct tcaccccatg
     9961 ctgaagtcgg agagtaagaa tttggttgac cataattttg aattaaagta tttttattaa
    10021 tagattttcc atcgagagag aagaaaacac cattagcttt attctcagat acaccataaa
    10081 gtgtttgatc gtataaattt attcgattgt caccagttgg attcttcgat atttgatagc
    10141 cgttgatagt aaaattatcg tatttcaatg actctttgaa atttttgtct aatataaatc
    10201 cactttgata cgcagtataa ccatcataat tataatgagg ttcaatagac tcttgcgcct
    10261 gtgcttgcgt tgcattaaat agttcgggcg ttacagagaa acccaaaaca ataccactag
    10321 ctagaacaat ttttgaaaat ttcttcataa aaaacctcct aaatatttat aattcatcta
    10381 tcattattac aaatatatta caaaaaatca tccgttatta caaaaacatt acaaaaagtt
    10441 tatctaaagt taatgcttta tcaacttcta aatttaaaac accaactatt ttttcgtgac
    10501 tatcatagtt aaactctgtt taattatggt ttctttttat gtagtgccta tagacaaatt
    10561 ggacatccaa aaagctttca tactgaaagt aaaattactc gcaataatct aatgatctta
    10621 atttcaattt taaaggggga tttcaaagta ttataaatcc aatttatagt ttaataccta
    10681 gaactttctg tctgctctca agacaagaag aagctcgttt ctaaaagcaa aaaaacaagc
    10741 cgagatttcg gcctattttc tttacataat taactattat gggtagtgcc tttgtataaa
    10801 tactcaaaaa cacaaacatt gttaaattaa tctttgtgtc ctttaatata agtaacataa
    10861 tagttaatta ttgcgatgag aaaaaccata gataactaaa tatataagca taataactat
    10921 gaaatgaata gctcctaaaa ataatgcgaa aatatctgta gtaagaggtt taaaataatc
    10981 tgatgaatcg taaatattca taaaccaatg aacacattcc caagaaagaa atgttccaat
    11041 acatctactt attataatac ttatattatt ttttaagccg aatttaaaaa tagatctact
    11101 tattaatata gcaactatta taaatgggat gtaacctatt aataaacctg tttgataatc
    11161 agtaaataat ccgataaatt ctattggaac taaagataaa attaatataa tgatttttaa
    11221 ccatttcatt ttaaaatttc tcttttcaat attatttttc cttcaatatc attttattaa
    11281 tataattagg taaaagtcta cgctgtcttt aagtgataga tgaatgatct ctatttttta
    11341 ttatgttata catttatatt attttctgta aggttctgat tctaaaagat atttataata
    11401 ggagtctttc ttttgtaaat ctgtttcccc ccatacctta tattctttat catctgtcca
    11461 tggtgctggt gctggtgtca cgttatactc atactcacta tatggttgat ctttaaaagc
    11521 aatatctttt tcagcatatc ccatcataat attaaaactc attttttcat tttttatttt
    11581 tttatcccaa cccttacgtt ctataacgtg atcgatgatt ttctcactct tatgttcctg
    11641 ataagtaatc acactaaaag tccaaactgt taaggtaata atgataacaa taatagaagt
    11701 tattaatatt tttaagattt tcttcatttt tgtataattc ctctttttta ataattttta
    11761 tgataaatat gtattattgt atttaatttt acaaaatatt aattaaataa atttacttaa
    11821 ggagactaat atgaaaagaa aatataaaat tacaggtatc atctttatcg ttcttcttat
    11881 cgttttaaca ttaattttta gtatagtgca ccattatgct aatgttcaaa aacatgaaga
    11941 agctaaacta aaacaaaaag ttcaccatat tttcaaacaa aaaggttggg aagataaagt
    12001 taaagaagaa aaaaatatat ttacgttcaa tactggagat aatgatttac aagtcacttt
    12061 taaagatgag ccttataata cgtatacata ttctattgat gaagacaata aagtatatgg
    12121 aaatgctgtt ttgaaagatg aatatgataa agattttgat agtaaaaaaa agtacaaaga
    12181 atatttaaga aaaatgcatt ttgaagaaaa atatgatctg aaataaaaaa gcaagcgact
    12241 acgataattg atagtcgctt gctttttagt gttctttcaa tttgtttaat gcttttttat
    12301 gattcgtttt ctatcttgta atttggaggc ttatatatgc cattttaaaa gcatatgcgc
    12361 gtctttaata tgaaatatac taaaattacc tatatgatat taaatgaaaa gcctgtataa
    12421 agtttaaaat agcttagaaa gcaaaaagga gtattagcat tgaaatcaat tgttcatttt
    12481 atttgcctct ttatcatatt ttgtattttt ggatatgtga tagatttaat attttttaaa
    12541 gaaataaata tttttctgat tttagaatca gcctttattt tatcattact aatcatgccg
    12601 atattaaaga ttgtaaaaga gatagaaatt gaaaaataga actggtaaat accattttct
    12661 atacttaatg tttaataaaa ataggctact aaaattcagt taataaagat ccatctttat
    12721 taactgaatt ttaaaaaata tatatagtgt taattttttt aattaaagta gttataactt
    12781 aatttatatg ataaagtcat atttaaacat ataaatattt tacaggaggt ctttttatga
    12841 aaaagttact ttcagttttt attgcaagtt tattagtttt aaccggatta tctttttctt
    12901 caattaatca aggtaatact gcgagtgcta aaacaaaata taaaacaact ataacttaca
    12961 aaggtcaaaa atatgtatat gttggtcatt ataaacacca tttttctaaa aaagaagtta
    13021 aaaaagtagt taaattttct aaaggagttc attcaggaaa caaattagta tctgctgcta
    13081 gtaaacttag taaaaatggt tatgttaaag catcctcggc tatttataaa gcctttgatt
    13141 ttggattgaa aaatgaatta aaaggtagct atttttacac agcagctaaa aaaggtacag
    13201 gcgtaacaat ttcttatgat atgtatactg caacaacagg tccgaaccat actatagcta
    13261 agaattttaa acatagttat aattaaataa atgtgagggg aaggattaat ccttcccctc
    13321 acatttattt tcaagctttt ttgaacggtt gaattttaga ccataattta tgaattcata
    13381 gatattagat aatatttcag ctattattct taaaatattt tccattatca tcactacttt
    13441 caattttatt cagtatattt aatttttctc tcgaattttt ttatatcccc atttttatat
    13501 ttaacttcat aaagaatact ttctcctaat aaatctttta cattgtcgta tttgtttgct
    13561 acacctttcc aaatcaatgt atctttagta tcttttttaa taccgtcatt agtaatttct
    13621 tttgcattgt tatttttata tcgatacact ttcccaattt tttcaggttt gttattttct
    13681 tttttattta ttgtaatatt aacagcttta cttgtaggat taatttctaa tgcacttttt
    13741 ttaattggtt tttctttaga actattacta catccagtaa cagttagaac taaaataaga
    13801 gaaataataa tactaaatat tgcttttttc attctaatca tccttttttg cttattcaag
    13861 attaccatat tcttcattta aatattttga tattaaattt tcaatttgtt tagatttatt
    13921 aataccatat tttgttttaa ctttatctaa attagttatt aatttaggag gaagagtaat
    13981 atttaacttt tgtctttgtt ctctttcatt caaacccaca acactctcct tttatttatg
    14041 cgtataaagt agcattacat gcgtacaaag tcaagtttgt acccataaaa gaagtatagt
    14101 atagtttgtt ttttacttta tggagctatt ataattacga caaacgataa aagaaaaaca
    14161 attttttcct ctcaaaaaag ccctatagtt ttcaactaag aataaatgag caaagcaaat
    14221 ggtaagatag tccttcagct atgctgaata ctaccttagg cggtaatgga tttccttatg
    14281 ctctttaaaa tacaaaagtt acattatata cctacttttt caggttttag cttttttcta
    14341 agaagaacaa ttgagataaa aaatatattt tcctttctga aagtgttgag aaagaaattt
    14401 taaaatgatt tatagcctat ctgttgaaca aaaaagtttt tagtcatttt ttaataaaat
    14461 gttctagaaa gggcttaaaa tgcaaaataa aaagactact caaaaaatga gtagtctgaa
    14521 gaatagtttt tgatgtatta aatgaaaggg agtactacat taatcatatc acacgcattt
    14581 ataccgagaa aatttattga tgttgagaag aacccttaac taaacttgaa gccgaatgtc
    14641 ggcatagcgt aagctattaa gccgacgatt cgataagttt agggattgtt aaagattcgg
    14701 aggttcaacg tcaataaagc aatttaaaac tatagttaaa atagggttct gttgcaaagt
    14761 cagaaagata agtatataac cttatgttta aaataatacg tcatattaac tcgctagcat
    14821 gatgctaatt tcacggcatg gtgaaaaccc gtagatctga agagacctgc ggttcttctt
    14881 atatagtcca taaatacatt caatgccttt gattgtattc tttgccgtat tgatactttg
    14941 ataacttatt ttccttactt tgataagacg cttaataaaa gcgtatgccg catgattatc
    15001 tcgttgctta cgcaaccaaa tatctaatgt gtggccgtct gcatcaatcg cacgatataa
    15061 atagcaccat ttgcccttaa ttttgatata tgtctcatcg acatgccatt tataataagc
    15121 ttttttatgc ttttgcttcc aaatttgata caaaatcggt gcatattctt gaacccaacg
    15181 atagactgtt gaatgatgaa cagtgacacc acgttctctt aatatttcag atatatcacg
    15241 ataactcaat gcgtatctta ggtagtagcc aacggctaca gtgatgacat ctttatcgaa
    15301 ttgtttatat ctgaaatagt tcatacagaa gactcctttt tgttaaaatt atactataaa
    15361 attaactttg caacagaacc ttgcttaaca tattcctata tgtaacgttc tttttgatac
    15421 acctgttatt tcaataatgt cttttaaatt atattggtat tccagaaatc attcacctct
    15481 tttaaaattt catctgatga tttaatagta gcttcttcta tagtacgttg tgttttttct
    15541 aaatagtatt ttataagata ataaccacac gtatatccgg ctgcatgtgg taatccaaca
    15601 ggcactccac cttgcattct tgtaatttca tctccataaa gatatggcat tgcctcaaac
    15661 atattattaa tatctagatt ttcctttatt gtatttttga ttttattatt ttgttgatcc
    15721 caatcaattt cagttaccca tggccctaaa tattttgttc catacatttt ttccacaaaa
    15781 ttttctgcga gtccttctga aataattaat tcagtcagag atccgccatc ccatttaatg
    15841 tactgatatc taatattatg atttatttcg tgagcaattg cacttttaat acggcttatt
    15901 gtatattgat tgggtactaa tgaaatgaat atataaccag gtatcccacc atctccacca
    15961 taattattat ttagatacat cgtgggtttt ttactgtccc ctaataatac agtaaacgta
    16021 tatttatcaa tatgtgattc aattccattt ttctcaaatt gttctatacc atcttctata
    16081 tataacttac aatcattcca aaaggcatca tttagcagtg caacttcact cctataattt
    16141 gtattaaatg cttctggcga aatatgagtc atatttaaaa attgtaatgc atcaaatcta
    16201 aagttgtcac tttcaaaagg tatgttctga atttcaaatt tttctttaaa gtattttaga
    16261 agattgtctt taaaatattt cgacttattt tcatattctg tatcaagtaa acctaaatat
    16321 accttatctg atctaactat atttatttta tacatattta tccctccata tcactaacaa
    16381 taaaaggttc tgttgcaaag ttaattttat agtataattt taacaaaaag gagtcttctg
    16441 tatgaactat ttcagatata aacaattcga taaagatgtc atcactgtag ccgttggcta
    16501 ctacctaaga tacgcattga gttatcgtga tatatctgaa atattaagag aacgtggtgt
    16561 cactgttcat cattcaacag tctatcgttg ggttcaagaa tatgcaccga ttttgtatca
    16621 aatttggaag caaaagcata aaaaagctta ttataaatgg catgtcgatg agacatatat
    16681 caaaattaag ggcaaatggt gctatttata tcgtgcgatt gatgcagacg gccacacatt
    16741 agatatttgg ttgcgtaagc aacgagataa tcatgcggca tacgctttta ttaagcgtct
    16801 tatcaaagta aggaaaataa gttatcaaag tatcaatacg gcaaagaata caatcaaagg
    16861 cattgaatgt atttatggac tatataagaa gaaccgcagg tctcttcaga tctacgggtt
    16921 ttcaccatgc cgtgaaatta gcatcatgct agcgagttaa tatgacgtat tattttaaac
    16981 ataaggttat atacttatct ttctgacttt gcaacagaac cactttaatt taccataaaa
    17041 acttaaatat tttgaggaga taaggagtct ttaaatgaaa aaaatagttt atatacttgg
    17101 tactattata gttttaggct tttgttattt ttatgtaaat ttaatttttt ttgatagttg
    17161 gacacgttat tctgctgaaa aagaaataaa taattatatt tcaaatcatg atacaaagaa
    17221 actaaaagaa atatccgcga ataataaaac atatcaactt ttgaaacatg ctaaacatgt
    17281 gacaataaaa ggagatacag ataatcaagg tggaggtcat atagggtatt ttactagtac
    17341 gattaatggt aaagaaggag cgctttttgt acatcttaat tttggtttgt ttcctgaaat
    17401 tcctaaagta accaagttgg aagtttttga taaaaatatt tatgagtagc ataattgcta
    17461 ctcatttttc gtgttagtta ttctggttct gttgcaaagt tagaaaaata tagctaatta
    17521 cgtccatata gcattaagct tctatgctat gataatcgtt agaaatctgg ctaagaatct
    17581 ggagtgtttt tatgattttt ttaagagaaa aaatagtcat gaaatataca aaaaaatcat
    17641 gtatattttg ttaaatagta cagtgatagc atttatatta ttgctaattg tgaaattaat
    17701 atttgatttc cttttttaga ctatattcat attttttcag atttgtattt ttattggggt
    17761 taattattgg tagtatccca gattttatag aaaagaaagg ttaattttat gttattaaaa
    17821 aataagattt attcaggcat tattattgtt atttttatag tttgtttgat tataggattg
    17881 aatgaaaata tcattggaaa tcctttagga gaaaaaatat tttatttatt aggttttcta
    17941 ttttttgtat tattaattat tggtacaaca aaaaatagaa agcattaatg tcttcttttt
    18001 gccccatttc ttacgataaa cattttttga gtagcctttt taatgttctt tcaatcgttt
    18061 aatttctttt tttaatggtc tatcatcacg atacgtatta aaattcataa atgcagtact
    18121 atcaaagtgc tctaacaagc gatgaaatat ctcattctcc cttttgtatt gttctatatc
    18181 atcttcaagt tggtcatgac gttgtcgaag tgcgtctctg tctgatttga gtgcttcgtt
    18241 gtcttcttct agatgttcaa taactttata caaattattc gtattattct tagaattaga
    18301 atcatccatc gttatttgct cttttggata ttcaatcacc gtcggtttaa tccgattacc
    18361 cacagctgtt tggcgtatat attctgtgac attcatacta cgtatgtagg ctaaatcttg
    18421 tatctttttg tattgcgctt gactgacacg aacggttaag attttttctt tcgagctttg
    18481 tttttttggc attgaatcac ctcataatta atatatgata agcatacata tgtgtgctta
    18541 tctttatatt aatataacat acatatgtat gttaataaat atgtatgatt cgaggtgttg
    18601 tgagctaaca tttggcatac atattatcaa acatatttgt ttgataatgc gtatgtcatt
    18661 tgttagctat agaacggaat tcaaaaatat atttttgaat gtagtgatgg cctcgccaga
    18721 gcgaagataa ctcttacatt tgaatgtaag agtatatcga gctatactct cattgcggaa
    18781 tcagcctcac atgtttgagg cgtagcggtg agagtatcca tcattttaca taagcgaagc
    18841 attgtcgaag gtagacaatc acagcgcttg taaaatgata tttgcgaagg ttctcgaaag
    18901 tagagaacca cagacaaatc aaaaagcttc gggaagggtg ctcaatttga aattagcttg
    18961 gaatacgcaa aaaataaaat gagcgatgtt aaaggcaaag agatggaaca agaacgtaaa
    19021 gggagaatca aaaacgatga gattgatttg aaacggacaa acttaaactt tgatttaatt
    19081 gaatatgaac gtcatttgta tcatcgtgtg aaagatagag tcgattatta taaagaacag
    19141 gggtcacgtg tacagaaaaa tagtgtggtg atgtattcca atatcattac gttatcgaaa
    19201 gaagaggccg accgtatggg agaaacacgt acaaaacact actttaaaac atgtaaagat
    19261 tacttcagtg aacgttttgg cgaagccaat gtcgtttcag cgaaagtgca tatggatgaa
    19321 tcagcgcctc atatgcacct tcactttata cctgtgaatc atcaagggcg gttatcagca
    19381 cgtacagcta tgaatcgaca agccattaat cacatccatg atgaactaac aacacatctg
    19441 tgccagcaag gttttgatgt tgaacgtggg agtaccgatg ataataaaac gtatattcaa
    19501 gatattcatg aatacaaaaa gaaagcgaaa gttttaagtg aattggacca acaaattgaa
    19561 acgaaaaaac aattgttgag ggatatcaac gatttacacg atgaaaaaga acatttaact
    19621 cgggatattc aaaacttaaa acggaaaaat cattttttac aagaggattc tcaacaggct
    19681 caagaagata tagacctata caagcaactg agaacgattt ctagggacga ttttaaaaaa
    19741 gatgaggaaa agtaccaaaa agaaatcaaa gcgtatgagg cgtctctgag cggccttaga
    19801 atgaattacc gagatttgaa caagcgcaat caacatttaa caacacaaaa taatcaactc
    19861 gaacaccatt tagacctgtt aaatgcacgt catcaagcga tgaacgacat ggtaagaaat
    19921 gatacacgta ttttgtttgc gggacttaga tgtgcattga aagataggaa cattgaatta
    19981 agcgcagaaa ttaaatatca acgtatgcca gaagtcggta ttgatcccga tacggtggat
    20041 cagggggctt taacacatgc atatcaaggt gatgtgtcag ggatagacca tcatataaga
    20101 aaggcatcag cacctattaa aaaagagaca gagaatgact tagaattaga ataagtcaat
    20161 aaaaataagc actcaggcaa gtatgatact tgtttgagcg cttatctcaa tgaaaagagt
    20221 aagtaaaaac ttaataagct ggcgacactt attaagagca ataataactt taattactga
    20281 attagtagac tagttcagta attaggaacg atacctttgt atttctatat tattataaga
    20341 attccaaggt agcaaagata ttgtattgga agtgataaat gtgagtatga tttttaaatt
    20401 aattgatgaa gatattttga gtttctaaac tttaaaaaat atctccattc ttttaaaagt
    20461 ttatttttgt gttggtttct ttataaatat tatttgaatg cgtttaaagt atttcactcc
    20521 gaccttttta atatattttt ataaatcttt ttaaaacctt tttaagtact gtttaagacc
    20581 tagagagaca aggcttttga aatgttaaag gcatagtttt gccacattaa aggcatagtt
    20641 ttgccacatt aaaggcatag ttttgccaca ttaaaggcat agttttgcca cattaaaggc
    20701 atagtttttt tattgttatg cctttatcta tatgttacct taattataag ggttatgagg
    20761 ttttttaaaa aattacaaag gcatagtttt tttattgtta tgcctttgta aagagaggag
    20821 tatcatatga gtggagaaac tgttatatat aaaaatgaaa tgaacttagt gcctttaaga
    20881 cgatttacag ctactgaaat caacttattt tttgcaatgt gtaataaatt aaaagagcaa
    20941 gacactaata cgttacactt atcctttgat gagctaaaag aattaagtaa ttataatcca
    21001 gaaacgcgta atataaacag atttgtagat gacctacaaa gagtttacga taaaatgcta
    21061 gatattcgat atactattag aacagaaaca aaaattaaaa aattcgttct attttataaa
    21121 tatgaaattg atatatctga aagatattta gaaattgcta cttccccaga tttaaaatat
    21181 attttaaact ctattactaa taactttact aaatttgaat taaaggaaat gacacatctt
    21241 aaatctactt atgccaaaaa tatgtttaga atgcttaaac aatataagca ttctggttat
    21301 atgaaaatta gggttgaaga ttttagagaa cgtttagata ttccaaattc ttatagaatg
    21361 tctaatatta atcaatttgt tctaactcca atcataagag aactatctac gatatttaat
    21421 aatctaaaaa tcaataaaat caaagctaaa aaaggacgta aaattgaatg gctggagttt
    21481 atctttgacg ctgagaaacg cattcacaat aaacgacaac ctcaaatgac caatgtaggt
    21541 aaacagcgtc aatatatcaa tcgtgagaaa acaccagaat ggttaaatga aggaagatta
    21601 ggacaatttc aagaaataca taatgaagat acaaaattaa aacaagatcg agaggcattt
    21661 caacgtcaat tagaagaaaa atgggaggat taataattta aatgagctac atgtatattt
    21721 tgcttttatt tcctttaata atgggattta tcgtatcaat aaaaaagaaa aaacatgact
    21781 aaggagacat aagaaggtat gagtaataaa tataatatac atccagcact atatatagga
    21841 ggatttgcgg tattttttag ttttatattt ggatttccta tagggacatt aatgggtcta
    21901 ggtataaggg agaaaactat ttaactcttt caacatggct atttgcattt tggggagtta
    21961 tgattattac tagtttttag tcagagattt ttctcaaggt acgtcacagt tgtatttgaa
    22021 t
//
