LOCUS       NZ_CP065655             6585 bp    DNA     circular CON 15-FEB-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_913 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP065655
VERSION     NZ_CP065655.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450443
            Assembly: GCF_016026915.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7
            Genome Coverage                   :: 2034.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 04:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,517
            CDSs (total)                      :: 2,433
            Genes (coding)                    :: 2,341
            CDSs (with protein)               :: 2,341
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 58 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 33 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065655.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_913"
                     /culture_collection="ATCC:12228-1"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed2"
                     /collected_by="NIST<ATCC"
     gene            269..1126
                     /locus_tag="I6G70_RS00050"
                     /old_locus_tag="I6G70_00050"
     CDS             269..1126
                     /locus_tag="I6G70_RS00050"
                     /old_locus_tag="I6G70_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458658.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_002498908.1"
                     /translation="MSGETVVYKNDMNLVPLRKFNNTEVNLFFTLCNKLKDKGNLTVNV
                     PFEELKELSNYYSHDKKLFIQDLEKFYDKVFSLTYREETENVIRKFILFTKVEIYKDKE
                     YVAIGVNPDLEHIINSLTSNFTKFELKEMTHLKSTYSKHMFRILKQYKHTGYVKIKIDD
                     FRERLDIPNSYRMTNINQKVLAPIIKELGFIFNNLNINKIKAKKGRKIEWLEFTFDAEK
                     RIHNKRQPQMANIDKSRQYISREKTPRWLEERTYEKQIKNEYDPQLEKERKAFLKQLQL
                     DWED"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(1174..1779)
                     /locus_tag="I6G70_RS00055"
                     /old_locus_tag="I6G70_00055"
     CDS             complement(1174..1779)
                     /locus_tag="I6G70_RS00055"
                     /old_locus_tag="I6G70_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002498907.1"
                     /translation="MNKSKVAEIRKQKSLTQENLAEKAYVTVRTIQRMEAGEEVSSETL
                     KSVSNALDVTVNELFESISSSDKEEEIMEISKEQQKQFNYRKNELFTIKLITFSFIFVF
                     LGLFGIFVGELNGLEQNIYRIIWIFALFLSLAIMYYFLNVFISKKLDKKYPMTIGMKNR
                     NRKNEPVKNGWDFMARYWWIVFPIGGFLSWLIPELTGK"
                     /name="helix-turn-helix domain-containing protein"
     gene            2513..2707
                     /locus_tag="I6G70_RS00060"
                     /old_locus_tag="I6G70_00060"
     CDS             2513..2707
                     /locus_tag="I6G70_RS00060"
                     /old_locus_tag="I6G70_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002447414.1"
                     /translation="MKKISKLLIAFSLVTTLSYASASTGFMNDNSHEAFAKSKKKKHKG
                     NGNKHNCFPVGNSEFCTTP"
                     /name="hypothetical protein"
     gene            2803..3603
                     /locus_tag="I6G70_RS00065"
                     /old_locus_tag="I6G70_00065"
     CDS             2803..3603
                     /locus_tag="I6G70_RS00065"
                     /old_locus_tag="I6G70_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498218.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_002498218.1"
                     /translation="MKSSLFILKEHFNNLYLMKRLAQFEMKISNKNNYLGTAWELINPS
                     IQIFMYWFIFGLGFRNNRTVENIPFIFWLIVGISMWFFVNQGILNGTKAVTSKYKQVAK
                     MNFPLSILSSYIVLSKFYTHVLLLILIMLICALNGYYPTIYTLQLLIFIPYTLLLTMTI
                     TLLTSTLAVIIRDIQMLMQALMRIIFFISSILYLPTNALVLKVIQFNPIYFIAESYRAA
                     ILYQNWFFITHWKLSLYNLAFLLITFVLGSILHVKYREQFADFL"
                     /name="ABC transporter permease"
     gene            3939..4325
                     /gene="mobC"
                     /locus_tag="I6G70_RS00070"
                     /old_locus_tag="I6G70_00070"
     CDS             3939..4325
                     /gene="mobC"
                     /locus_tag="I6G70_RS00070"
                     /old_locus_tag="I6G70_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_011117491.1"
                     /translation="MSEQNTFVASDETVGRNRKPNRKAPKQISFRVSESEYLKLQQSAE
                     TLNMSVPAFVKKKAQGARLVAPKLDQATRQSVAKDLSMLGANANQIAKYCNQHQHEAPN
                     YEALERNISELRERLDEIWNMIQK"
                     /name="mobC"
     gene            4491..5501
                     /locus_tag="I6G70_RS00075"
                     /old_locus_tag="I6G70_00075"
     CDS             4491..5501
                     /locus_tag="I6G70_RS00075"
                     /old_locus_tag="I6G70_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002498904.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARE
                     LYGKTDGNQGHVIIQSFQPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHI
                     VLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGLSVPEKDTARLRYTQTEKAIADPN
                     TTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSITYRHLAENKKVR
                     GRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSDW
                     DQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5404..6009
                     /locus_tag="I6G70_RS00080"
                     /old_locus_tag="I6G70_00080"
     CDS             5404..6009
                     /locus_tag="I6G70_RS00080"
                     /old_locus_tag="I6G70_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_077431415.1"
                     /translation="MKRLEEIEKREYERNQRHDQLLTSIETTASNFNQATDFTQKRFIS
                     AARHYIERINNDNLKQDFHTAIQKELEDVKTDTQNVVKEVQNNQSELRQANNNYKKMID
                     ERMKSNAIVIKQYDQVIHRLTKGITAMFFIVALVMVAFLVISPLGDWLGVQHFYEWLNH
                     VLKTGHSAWRYFMLVFYLVPYILFGVLIYAILKAYERL"
                     /name="mobilization protein"
     Typing          complement(3730..3968)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.72e-122"
     Typing          303..1126
                     /name="rep_cluster_1256"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4483..5498
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.705"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttaggag tcatttgatc cttagagtga caatagattt gaaatgttaa aggcaaccaa
       61 tcattaaact aaaggcaacc aatcattaaa ctaaaggcaa ccaatcatta aactaaaggc
      121 aaccaatcat taaactaaag aaactttttt tatattaggt ttctttatgt tattattgtt
      181 tataacatag tgattataag tatttacaat taaagttaat aggaatcttt attctatata
      241 ggtttctttt agattggagt ggagttagtt gagtggagaa acagtagtat ataaaaatga
      301 tatgaattta gttccactga gaaaatttaa taatactgaa gttaatctat tttttacttt
      361 gtgtaataaa ttaaaagata aaggcaattt gactgttaat gtgccatttg aagaattaaa
      421 agaattaagt aattactata gtcatgataa aaagcttttt attcaagatt tagaaaaatt
      481 ctatgacaaa gttttctctt taacatatag agaagaaact gagaatgtta ttagaaaatt
      541 tattcttttt actaaagtag aaatttataa agataaagag tatgtggcta ttggtgttaa
      601 tccagattta gaacatatta ttaattcatt aactagtaac ttcactaaat ttgaacttaa
      661 agaaatgaca caccttaaat ctacatattc taagcatatg ttcagaatac ttaagcagta
      721 taaacatact ggctatgtaa aaattaaaat tgatgatttt agagaacggc tggatattcc
      781 taatagttac cgtatgacaa atattaatca aaaagtatta gcccctatca ttaaagaatt
      841 aggctttatt tttaataacc ttaatatcaa taaaataaag gcaaaaaaag gacgtaaaat
      901 tgaatggtta gagtttactt ttgacgctga gaaacgcatt cacaataaac gacagccaca
      961 aatggcgaat atagataagt cacgacaata tataagtcgg gaaaaaacgc cgagatggtt
     1021 agaagaacgt acttatgaaa aacaaataaa aaatgaatat gatccacaat tagaaaaaga
     1081 gcgaaaagca tttttaaaac aacttcaatt agactgggaa gattaaaaca aaagatcttt
     1141 agcccccttg ctaaagatct tttgttttgt attttatttt cctgttaatt caggaattag
     1201 ccaacttaga aaccctccaa taggaaaaac aatccaccaa tatctagcca taaaatccca
     1261 gccatttttt acaggttcat tttttctatt tctatttttc attccaatag tcataggata
     1321 ttttttatct aatttttttg aaataaaaac atttaaaaaa taatacatta ttgctaagct
     1381 caaaaataaa gcgaaaatcc aaatgatgcg gtaaatattt tgttctaaac cgtttaactc
     1441 acctacaaag attccaaata gtcctaaaaa cacaaaaata aaactaaatg ttattaattt
     1501 aatagtaaaa agttcatttt tacgataatt aaactgcttt tgttgttctt tagatatttc
     1561 cattatttcc tcctccttat cagaagacga gatagattcg aatagttcat tgactgtcac
     1621 atctaatgcg tttgagacac ttttaagtgt ttcactacta acttcttctc cagcctccat
     1681 tctttgaatt gttcttactg ttacataagc cttttctgct aaattttctt gagttaacga
     1741 tttttgtttt ctaatttctg caacttttga tttgttcata ctcataaact actcctcaac
     1801 taaaaaaatg aacacgaaaa attgccgaca gatgggcgac aatgaaacga cagttacttc
     1861 aaaattatat tcttaaaaga aagtatatat ctaattttac aaaaagcata agaaaggata
     1921 tactgaaagt aacaaatagc agatatatca ttgtatgtga aaacccccag cgtgccggct
     1981 gggggtttct ttttacttat gctcttgttg taatagataa agaacataac caacaataat
     2041 tggaaaaatg agcttatcta gtacaaatag caagattatc attgtatgtg acacctcctt
     2101 tgtgcacgga agtgcttttc cagtataaca aaaaactaca atttaaaacg gtgtgttatc
     2161 acgtgaaatt gtgaataaag atacaaaagt tcacttaatg aaaaagaatg aaaacacgtg
     2221 agaacaacga tgtataaaag atgaataaac gataatcaca agacaaaggc ttaaacgttg
     2281 atttaacagc gtttaagcct ttattgttag ttgacataat tactgattat aggtagttag
     2341 tttgtataga atacgtataa tttgtaaaat tgaatagaat attaaaaaat tctatatttt
     2401 actcttcaaa aatatatgct aaaaccaatt aataaattta tatttttttc gtcttgattt
     2461 ttcttaaaat tgtgatatct ttactttaaa ttaatatttt ggaggggtag aaatgaaaaa
     2521 aatctcgaaa cttcttattg cattttcatt agtaacaaca ctatcatatg caagtgcttc
     2581 aactggtttt atgaatgata actctcatga agcatttgct aaatctaaaa agaaaaaaca
     2641 taaaggaaat ggtaacaaac ataattgctt cccagtcgga aacagtgaat tttgtacaac
     2701 tccataatta aaatagaaaa ggtaaagcaa taaacaattg ctttaccttt tttttaggtt
     2761 tcatttataa tattgataat cttagaaagg taacttaatg ttatgaaatc atctttgttt
     2821 atattaaaag aacattttaa taatttatat ttaatgaaaa gattagcaca atttgaaatg
     2881 aaaatttcta acaaaaataa ttatttggga acagcatggg aattaattaa tccttcaata
     2941 cagattttta tgtattggtt tatttttggt ttaggtttca gaaataatag aactgttgag
     3001 aatatacctt ttattttttg gttaattgta ggaataagta tgtggttttt tgtgaatcaa
     3061 ggtattttaa atggtacaaa agcagttact agcaaatata agcaagtagc taaaatgaat
     3121 tttccattat ctatattatc tagttatatc gtcttaagta aattttatac ccatgtgtta
     3181 ttgcttattt taattatgct tatatgtgct ttaaatggat attaccctac tatatataca
     3241 ttacaattat taatttttat cccttacact ctgttactga caatgacaat aacactatta
     3301 acttcaacat tagctgtaat tattagagac atccaaatgc ttatgcaagc tcttatgaga
     3361 ataatatttt ttatatcttc aattttatat ttaccgacaa atgctttagt tttaaaggtt
     3421 atacaattta atcctatcta ttttatagct gaaagttata gagcagcaat tctatatcaa
     3481 aattggtttt ttataacaca ttggaaacta tctttatata atcttgcttt ccttttaatt
     3541 acttttgtgt tgggctctat tttacatgtt aaatatcgtg aacaatttgc agatttttta
     3601 taaacagaat ataaagaaca ttatttttga tcttattctc atttcttttt taattttatt
     3661 tttcatttgg gtttaataat tatagcaaaa atatttttgg ttgctgcggt tttgatggtt
     3721 ttgaatttag tggctagcaa ttagccacta ttttttcgtt agaaaaaatc cataaggggc
     3781 ttggggatta cccccaacaa gcaggcgcgt ctgccacgtt agtggctagc aaagccaatg
     3841 cttgccaaac cacttaacga cttgatgaat cttactttat catataaagt aagatgtaag
     3901 aacggcgtta tctttcgaag aaagcgtggt gggattgaat gagcgaacaa aacacatttg
     3961 tggctagcga cgaaactgtt gggcgaaacc gtaaacccaa ccgtaaggcg ccgaaacaaa
     4021 ttagttttcg tgtgagcgaa tccgaatatt taaagttaca acaatcagct gaaactttaa
     4081 atatgagtgt tcctgcgttc gttaagaaaa aggcacaagg cgcccgattg gtcgcaccca
     4141 aattggatca agcaacgcga caatcggtag ctaaagattt aagtatgttg ggtgcaaatg
     4201 ccaatcagat tgcgaaatat tgcaaccaac atcaacacga agcaccgaac tacgaagcat
     4261 tagaacgtaa cattagtgaa ttacgtgaaa ggttagatga gatatggaac atgatccaaa
     4321 aataaaaatg attgtaagtg ccattttatt tgtgatgtgt ttaatgatat tgttttattt
     4381 tattttcttt gatccacaat ttccggtgat tttgtgtttt tcacttttaa cttttatcac
     4441 aggtattgat ttatatatat catataaaaa gaaaaaagcg cgtgatacca atggcaacaa
     4501 ctaaattaag tgcgactaaa tcaacatcac gggccatcaa ttacgctgaa aaacgtgccg
     4561 ttgagaaaag tggcttaaat tgtgacgtgg actatgccaa atctagtttt aaagccgctc
     4621 gtgaattgta tggcaaaaca gacggtaatc aagggcatgt cattatccaa tcgtttcaac
     4681 ctggcgaagt gacgcccgaa caatgtaacc aattaggatt agcgcttgct gaaaaactcg
     4741 tccccaatca tcaagtcgcc gtctatacgc atgcagatac aaaccatgta cacaatcata
     4801 ttgtacttaa ttcaattgac ctcaatacag gtaagaaatt taacaataat aaacaagccc
     4861 tacgcgatgt gagagcgttc aacgacgagg tttgtcgagc acaggggttg agcgtgcctg
     4921 agaaagacac agcacgctta cgttacaccc aaacagaaaa agcgattgcc gaccctaaca
     4981 cgacgtcgac agcacaatac tcatggaaag atgacatacg tgaggcaatt gaccaatcaa
     5041 cagcgacaaa tatggaagaa tttcaaaaac atctgaatca acatggtatt acgattgaac
     5101 gtgttacccc caaaagcatt acctatcggc atttagctga aaataaaaaa gtacgcgggc
     5161 gtaaattggg cgaagattac aataaaggag gcatccaaca tggctttgaa agacaaatac
     5221 aacgacgaca acaacaaaaa cataaatcag acacgaactt ccagccaaaa cgatcaactt
     5281 caaaccgtca tgaatcaact gaacgagata caggcgtcac tcaatcagat tgggaccaat
     5341 tcgcccaaga cacaaatgag cttgagcgtc gccgacaagc tgcagaatca gcaagacttg
     5401 ttgatgaaaa ggctcgaaga gatcgagaaa agagagtacg agagaaacca acgtcacgac
     5461 caattattaa catcgatcga gaccacggcc tcgaacttta atcaagccac agacttcaca
     5521 caaaagcgtt ttataagcgc ggcaagacat tatattgaac ggattaataa cgataattta
     5581 aaacaagatt ttcacacagc tatacaaaaa gaattagaag atgtcaaaac agacacacaa
     5641 aacgtagtta aggaagttca aaacaaccaa tccgaactgc gacaagccaa taataattat
     5701 aaaaaaatga ttgatgaacg tatgaagagc aacgcaatag tgattaaaca atatgatcaa
     5761 gtcatacatc gtttaactaa aggcatcaca gccatgtttt ttatcgttgc cttagtgatg
     5821 gtcgcctttt tagttataag tcccttaggt gactggttag gcgtccaaca tttttacgaa
     5881 tggctgaacc acgtgcttaa aacggggcat tcagcatggc gttatttcat gcttgttttc
     5941 tatcttgtac cttatatttt atttggagtg cttatatatg ccattttaaa agcatatgag
     6001 cgtctttaat acaaaataaa tagaaaatta ccttttgttt aatgaagaag ctatatcaag
     6061 cttaaaatag cgtagaaacc aaaaaggagt gctcgccttg aattctattg ttcactttat
     6121 ttgttttttt attgtatttt gtctttttgg atatgtggtc aacttaatat tttttgaagg
     6181 aacaaatatt tttctgattt tagaatcagc atttatttta tcattactaa tcacaccgat
     6241 attaaaggtt gtaaaagata tggaaaatga agaatagcac atgcgcttat gccgagaaaa
     6301 tttattgagc attgagaaga acccttaatt aaactgtttc gacgaatgtc gggatagtgt
     6361 gagctatgaa accgacgagt cgacagtttt agggtttatt aagggttcag aggctcaatg
     6421 tcaataaagc aattggaata atgcattaaa gttatatcac ttattacttt tttaaataat
     6481 aaaaacccac cacaaatgtg gtgggtttcc tttttgtctt ttgaatttgt gaggggcaaa
     6541 ttgccccgaa ctttttaata tctttttata aatcttttta aaacc
//
