LOCUS       NZ_CP065654            11858 bp    DNA     circular CON 15-FEB-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_913 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP065654
VERSION     NZ_CP065654.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450443
            Assembly: GCF_016026915.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11858)
  AUTHORS   Jackson,S., Kralj,J., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7
            Genome Coverage                   :: 2034.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 04:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,517
            CDSs (total)                      :: 2,433
            Genes (coding)                    :: 2,341
            CDSs (with protein)               :: 2,341
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 58 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 33 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065654.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_913"
                     /culture_collection="ATCC:12228-1"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed1"
                     /collected_by="NIST<ATCC"
     gene            complement(22..790)
                     /locus_tag="I6G70_RS00005"
                     /old_locus_tag="I6G70_00005"
                     /pseudo=""
     CDS             complement(22..790)
                     /locus_tag="I6G70_RS00005"
                     /old_locus_tag="I6G70_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1315..2550)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00010"
                     /old_locus_tag="I6G70_00010"
                     /pseudo=""
     CDS             complement(1315..2550)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00010"
                     /old_locus_tag="I6G70_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     ARG             complement(7851..8311)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.34"
                     /coverage="33.33"
     ARG             complement(8306..8550)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.75"
                     /coverage="17.65"
     gene            complement(4277..5221)
                     /locus_tag="I6G70_RS00020"
                     /old_locus_tag="I6G70_00020"
     CDS             complement(4277..5221)
                     /locus_tag="I6G70_RS00020"
                     /old_locus_tag="I6G70_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(5748..6989)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00025"
                     /old_locus_tag="I6G70_00025"
     CDS             complement(5748..6989)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00025"
                     /old_locus_tag="I6G70_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     ARG             complement(11600..11856)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="18.52"
     ARG             complement(2740..4115)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8712..9618)
                     /locus_tag="I6G70_RS00035"
                     /old_locus_tag="I6G70_00035"
                     /pseudo=""
     CDS             complement(8712..9618)
                     /locus_tag="I6G70_RS00035"
                     /old_locus_tag="I6G70_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(10177..11413)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00040"
                     /old_locus_tag="I6G70_00040"
                     /pseudo=""
     CDS             complement(10177..11413)
                     /gene="mobV"
                     /locus_tag="I6G70_RS00040"
                     /old_locus_tag="I6G70_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            complement(11599..>11835)
                     /locus_tag="I6G70_RS00045"
                     /old_locus_tag="I6G70_00045"
                     /pseudo=""
     CDS             complement(11599..>11835)
                     /locus_tag="I6G70_RS00045"
                     /old_locus_tag="I6G70_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             complement(7438..7853)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.28"
                     /coverage="30.07"
     ARG             complement(7175..7398)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="16.12"
     Typing          complement(24..151)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.154"
                     /coverage="99.0"
                     /evalue="1.74e-30"
     Typing          complement(170..790)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4278..5221)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8713..9653)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.683"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1955..2550)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.975"
                     /coverage="99.0"
                     /evalue="1.2e-138"
     Typing          complement(5752..6989)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10261..10709)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.02e-96"
     Typing          complement(10904..11259)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.958"
                     /coverage="99.0"
                     /evalue="1.98e-80"
ORIGIN
        1 tcagtaatat taatatgtac actatttcca aaatttaaat tcatgtgcca aaaatcgatt
       61 tgtttttgca attgtttttc gttcgctttt aaagtcgatt tcattaagtc cgttaaatcg
      121 actggcgaaa tttcttttat caaattctta tccactagcc attcataaaa atgttcagac
      181 cataacttca gcatctgcat tatctttacg ttcttgcttt ttattataaa ttctaataat
      241 ctattactat ctctcacgcc aaaatatttt gtttctggct taccattacg accataaaaa
      301 tagttttctt aactgcttta tcaacattgc atagtagtca ctcaaatcat cttcaaaatc
      361 aaaggctaaa tctatcttgt aaaaccgtca tcttccatgt agcttattat attttgtttt
      421 aaccaaatca tttcatctcg tgtaagtttg tttggattaa attcaatacg catattacgt
      481 ctatcccaac tatctgcttt cactttgtca tattcaatat aactttttct tgaagtgctt
      541 tagctttaaa ctttgtttga agatatccca aagtcttatt tggggctcta cactcataaa
      601 tttagaaagg gcttgagcgt tgtctcggtt gagatttcca acgatcgtca ttgcatcaaa
      661 acttattttg gatcagatat gcacacagta tgtgcgtcca accggctatt agagtagccg
      721 gttttagaaa aattgtctaa tcgtgatttt ccaaatgatt tgaatgattt gcatgattgt
      781 ttttatacat aaaaatcgac tccttaatct caatttcgtt taaggaatcg ctcacccaaa
      841 tatatatctt gatgtatatt taaatatcgt ttaatatcta aatatacaag attataaaaa
      901 caactcagtg tttttttctt tgaatgatgt cgttcacaaa ctttggtcaa ggcgtgagcg
      961 actccttttt attttgttat taatataaca ctatcaaaag acttggtcta atcagatcaa
     1021 gtctttttta tttaagcatt tgtattatct ggtaaacagt taaaactact aaaacaccaa
     1081 gtacatactt acttgttata aaattctcaa aggcttctct gaatttcatt tcttcaactc
     1141 cttttattgc tttattccaa tttccttatt ggtcggaacc tacagggctt tatgtgttgt
     1201 tgagttggta ctttcttggg attaatccca attcaagtcc aaccaactcg ctaacaagtt
     1261 agctaacaca tagcccattc caaccaataa gttttctcgg cataaatgca tgttctataa
     1321 gtgcatacct ctatctcgtt ttgattcttt tgttgttctt gttgctgttc ctgttctgaa
     1381 tataattctg gattcatttt ttatctaagt ttcctgctaa accatttagt tttggttcat
     1441 cttctgttaa cttattaact ctttccgcaa aggtatttct tcctaaactc gttctaactc
     1501 ttttagtaag atttttaaaa gctttaacgc tatttctata ttctctttaa gtggctttgc
     1561 attttcgtaa agttggttaa aattatcgtc tgcgttttca atacgctcaa ctgctttatt
     1621 taataaatca tctttctctc gtatttcctt atctttatca tctaaggctc taccagactt
     1681 tatatactcg taatcttccg aatatcttga gcagctttta tctgtttctg aaattcattg
     1741 aaatcttttt ggcttattac aacatttcca gtttcttgta tttctttgct aaataaacca
     1801 cctacttttc agtttcttgc tcatacggaa catttatagg ctttttaagc gtatttaacg
     1861 atttttggta ctcttgcatt aatttatcgt tcttttgctt tatatggtct gttttttggc
     1921 tctcacgttc atattcttgc ttatgatatt ctgtttttgt ttatactgac ttatttgctc
     1981 atgtttagca tttgttactt gtcttgattg cccacgttct aaatcatatc ctcgttgttt
     2041 aacatgctca ttaaatctat cttgaaacgc tgttaaagct tttttattac ctacaacttc
     2101 tttagcactt aaacgaccat catcagttat tggaacaacg ccataatgca tatgtggtgt
     2161 tttttcgtcc atgtgaactg ttgcatataa taaattatct ttaccgtatt cttgttctaa
     2221 aaactcttta gcatattcaa aaaactgctt tgtatcttct ggcgtttgat tatcaaagaa
     2281 atcattgtct gatgtaatta aaccatcaat gtgtttaatc gcgtctgttc taatttttct
     2341 tttgcctgta taattctgtt cgattttttc atcaatcaag ttattaaaat tctgtttatt
     2401 agcatttacc aaatcataat ttaagtaagt tttactatgg tctatatctt cattttcata
     2461 attattattt tctctttgaa catgtttttg tatgcccgtt gtatttgttc cagatttaac
     2521 ttttgaaact ctaacaatgg aataagacat ataaaaatca ctcctaagca caaaggtttc
     2581 atgtgtattt aatagtttaa gtctaacaca ctagacttat ttttaataag tcgttaatac
     2641 gtgtgctctg cgaggcttaa acctgttttt gctaacgcaa aaaatgagtg gcaaaatgct
     2701 agccactcat agttctaaac caaaatataa tataactatt caaactgctt ttcagaacgt
     2761 ttaaatacaa taatcgtcaa aagacaacat aaaataataa ggatagccat ggctacaaga
     2821 atattactat acactccaga agaataattt ataaattcca gaactagttt acgattaatc
     2881 aattgtagtg acaataaacc tcctacaatt gctatacctg ttccctctga taaaaaactt
     2941 gtgaaattta gcaaactcat tccagaagca acttcttctt cagaaagact acttgatact
     3001 atttttgata taactgtttt agtaaaagat aatccgccca taacaaatat aaacataaaa
     3061 gtagtcaacc acatactaaa ctcaacaaaa aatgcaatag ttaaaaaact tatagagata
     3121 gacaatgatc ctaaaataaa aacaaataat gatccttttc tatccactaa aaaaccacca
     3181 aaataaccaa aaacaataac actcatggtt ccaggaaaaa taacactatt acctattgtc
     3241 gctacattta catgataaat agttttcatc atataaggca ccattgatat aaaaccagct
     3301 actatagaaa atattagccc accagaaaac aaaccaagca taaacggaat gtttttccct
     3361 agtttaggat taataaaagg gttagaaact cttgaaatat gtttaataaa aatcacaaaa
     3421 aagattgtga agagtattaa aaaagtccaa ttataatttg tcgtaaataa cataaaacat
     3481 ataatactta tagacattaa aacaatacct acgatatcta atgtattttt tgttgattta
     3541 ccaggtacca ttactttaat aagaaaaggt atagttacta ttgtaatcat aggaagtata
     3601 agtaggtaag accaatgaat ataatgtgct attattcccc ctattgaagg acctaaccct
     3661 tcacctaaag ctacaattga tcctataaaa ccaaaggctt tgccttgttt ttttcttgta
     3721 atatttctag ctacaaccac cataatcagt gaagggaatg cagcagatcc tactccttgt
     3781 actaacctac caaaaatcaa aataaaaaag tgattgtgac caataaaagc aatcaatgaa
     3841 ccaagacagc tcaaactaat accaataatt aacaattttt ttatatttat ataatcagat
     3901 aattttccat atactgctgt tcctatcgaa aaagttaaca tatatgcagt gtttacccag
     3961 tttgtaattc caggagtagt attaaaatga tttgcaatat caggtaaaga aacatttaaa
     4021 accatttcat ttaatacact aaaaaatgaa agaatacaaa gccaaaataa aacgctataa
     4081 aacaaacctt taaatttttt atataaacta aacaatttta cctctttact tgaggtgact
     4141 aaagtttata ggggtaaatt attctttcac ctcatataaa ttccccaaat ttttaaatag
     4201 acacttcata aaaaaatcct cctaaattag aatagtttaa atatatcatt tttgttcagt
     4261 aatattaata tgtacactat ttccaaaatt taaattcatg ttgccaaaaa tcgatttgtt
     4321 tttgcaattg tttttcgttc gcttttaaag tcgatttcat taagtccgtt aaatcgactg
     4381 gcgaaatttc ttttatcaaa ttcttatatt ttgttctaga atttctgtga agctttcccc
     4441 attcttcttc atcactcaat aacataaaaa ctattgctct atccgcagtg cgttggatag
     4501 ttttccaatc tggttgcaag atatgtaaat cactaaagca atcattccag taatccacca
     4561 tatctctttt aagttcgatt tctacacgcc ataaatgttc agacataact tcagcatctg
     4621 cattatcttt acgttcttgc tttttattat aaattctaat aaatctatta ctatctctca
     4681 cgccaaaata ttttgtttct ggcttaccat tacgaccata aaaaatagtt ttcttaactg
     4741 ctttatcaga cattgcatag tagtcactca aatcatcttc aaaatcaaag gctaaatcta
     4801 atcttgtaaa accgtcatct tccatgtagc ttattatatt ttgttttaac caaatcattt
     4861 catctcgtgt aagtttgttt ggattaaatt caatacgcat attacgtcta tcccaactat
     4921 ctgctttcac tttgtcatat tcaatataaa ctttttcttg aagtgcttta gctttaaact
     4981 ttgtttgaag aatatcccaa agtcttattt ggggctctac actcataaat ttagaaaggg
     5041 cttgagcgtt gtctcggttg agatttccaa cgatcgtcat tgcatcaaaa cttaattttg
     5101 gatcagatat gcacacagta tgtgcgtcca accggctatt agagtagccg gttttagaaa
     5161 aattgtctaa atcgtgattt tccaaatgat ttgaatgatt tgcatgattg tttttataca
     5221 taaaaaatcg actccttaat ctcaatttcg tttaaggaat cgctcaccca aatatatatc
     5281 ttgatgtata tttaaatatc gtttaatatc taaatataca agattataaa aacaactcag
     5341 tgtttttttc tttgaatgat gtcgttcaca aactttggtc aaggcgtgag cgactccttt
     5401 ttattttgtt attaatataa cactatcaaa agacttggtc taatcagatc aagtcttttt
     5461 ttatttaagc atttgtatta tctggtaaac agttaaaact actaaaacac caagtacata
     5521 cttacttgtt ataaaattct caaaggcttc tctgaatttc atttcttcaa ctccttttat
     5581 tgctttattc caatttcctt attggtcgga acctacaggg ctttatgtgt tgttgagttg
     5641 gtactttctt gggattaatc ccaattcaag tccaaccaac tcgctaacaa gttagctaac
     5701 acatagccca ttccaaccaa taagttttct cggcataaat gcatgttcta taagtgcata
     5761 cctctatctc gtttttgatt cttttgttgt tcttgttgct gttcctgttc tgaatataat
     5821 tctggattca tttttttatc taagtttcct gctaaaccat ttagttttgg ttcatcttct
     5881 gttaacttat taactctttc cgcaaaggta tttcttccta aaactcgttc taactctttt
     5941 agtaagattt ttaaaagctt taacgctatt tctatattct ctttaagtgg ctttgcattt
     6001 tcgtaaagtt ggttaaaatt atcgtctgcg ttttcaatac gctcaactgc tttatttaat
     6061 aaatcatctt tctctcgtat ttccttatct ttatcatcta aggctctacc agactttata
     6121 tactcgtaat cttccgaaat atcttgagca gcttttatct gtttctgaaa ttcattgaaa
     6181 tctttttggc ttattacaac atttccagtt tcttgtattt ctttgctaaa taaaccacct
     6241 actttttcag tttcttgctc atacggaaca tttataggct ttttaagcgt atttaacgat
     6301 ttttggtact cttgcattaa tttatcgttc ttttgcttta tatggtctgt tttttggctc
     6361 tcacgttcat attcttgctt atgatattct gttttttgtt tatactgact tatttgctca
     6421 tgtttagcat ttgttacttg tcttgattgc ccacgttcta aatcatatcc tcgttgttta
     6481 acatgctcat taaatctatc ttgaaacgct gttaaagctt ttttattacc tacaacttct
     6541 ttagcactta aacgaccatc atcagttatt ggaacaacgc cataatgcat atgtggtgtt
     6601 ttttcgtcca tgtgaactgt tgcatataat aaattatctt taccgtattc ttgttctaaa
     6661 aactctttag catattcaaa aaactgcttt gtatcttctg gcgtttgatt atcaaagaaa
     6721 tcattgtctg atgtaattaa accatcaatg tgtttaatcg cgtctgttct aatttttctt
     6781 ttgcctgtat aattctgttc gattttttca tcaatcaagt tattaaaatt ctgtttatta
     6841 gcatttacca aatcataatt taagtaagtt ttactatggt ctatatcttc attttcataa
     6901 ttattatttt ctctttgaac atgtttttgt atgcccgttg tatttgttcc agatttaact
     6961 tttgaaactc taacaatgga ataagacata taaaatcact cctaagcaca aaggtttcat
     7021 gtgtatttaa tagtttaagt ctaacacact agacttattt ttaataagtc gttaatacgt
     7081 gtgctctgcg aggcttaaac ctgtttttgc taacgcaaaa aatgagtggc aaaatgctag
     7141 ccactcatag ttctaaacca aaatataata taactattca aactgctttt cagaacgttt
     7201 aaatacaata atcgtcaaaa gacaacataa aataataagg atagccatgg ctacaagaat
     7261 attactatac actccagaag aataatttat aaattccaga actagtttac gattaatcaa
     7321 ttgtagtgac aataaacctc ctacaattgc tatacctgtt ccctctgata aaaaacttgt
     7381 gaaatttagc aaactcattc cagaagcaac ttcttcttca gaaagactac ttgatactat
     7441 tttgatataa ctgttttagt aaaagataat ccgcccataa caaatataaa cataaaagta
     7501 gtcaaccaca tactaaactc aacaaaaaat gcaatagtta aaaaacttat agagatagac
     7561 aatgatccta aaataaaaac aaataatgat ccttttctat ccactaaaaa accaccaaaa
     7621 taaccaaaaa caataacact catggttcca ggaaaaataa cactattacc tattgtcgct
     7681 acatttacat gataaatagt tttcatcata taaggcacca ttgatataaa accagctact
     7741 atagaaaata ttagcccacc agaaaacaaa ccaagcataa acggaatgtt tttccctagt
     7801 ttaggattaa taaaagggtt agaaactctt gaaatatgtt taataaaaat cacaaaaaga
     7861 ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt aaataacata aaacatataa
     7921 tacttataga cattaaaaca atacctacga tatctaatgt attttttgtt gatttaccag
     7981 gtaccattac tttaataaga aaaggtatag ttactattgt aatcatagga agtataagta
     8041 ggtaagacca atgaatataa tgtgctatta ttccccctat tgaaggacct aacccttcac
     8101 ctaaagctac aattgatcct ataaaaccaa aggctttgcc ttgttttttt cttgtaatat
     8161 ttctagctac aaccaccata atcagtgaag ggaatgcagc agatcctact ccttgtacta
     8221 acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat aaaagcaatc aatgaaccaa
     8281 gacagctcaa actaatacca ataattaaca attttttata tttatataat cagataattt
     8341 tccatatact gctgttccta tcgaaaaagt taacatatat gcagtgttta cccagtttgt
     8401 aattccagga gtagtattaa aatgatttgc aatatcaggt aaagaaacat ttaaaaccat
     8461 ttcatttaat acactaaaaa atgaaagaat acaaagccaa aataaaacgc tataaaacaa
     8521 acctttaaat tttttatata aactaaacaa ttttacctct ttacttgagg tgactaaagt
     8581 ttataggggt aaattattct ttcacctcat ataaattccc caaattttta aatagacact
     8641 tcataaaaaa atcctcctaa attagaatag tttaaatata tcatttttgt tcagtaatat
     8701 taatatgtac actatttcca aaatttaaat tcatgttgcc aaaaatcgat ttgtttttgc
     8761 aattgttttt cgttcgcttt taaagtcgat ttcattaagt ccgttaaatc gactggcgaa
     8821 atttctttta tcaaattctt atattttgtt ctagaatttc tgtgaagctt tccccattct
     8881 tcttcatcac tcaataacat aaaaactatt gctctatccg cagtgcgttg gatagttttc
     8941 caatctggtt gcaagatatg taaatcacta aagcaatcat tccagtaatc caccatatct
     9001 cttttaagtt cgatttctac acgccataaa tgttcagaca taacttcagc atctgcatta
     9061 tctttacgtt cttgcttttt attataaatt ctaataatct attactatct ctcacgccaa
     9121 atattttgtt tctggcttac cattacgacc ataaaaaata gttttcttaa ctgctttatc
     9181 agacattgca tagtagtcac tcaaatcatc ttcaaaatca aaggctaaat ctaatcttgt
     9241 aaaaccgtca tcttccatgt agcttattat attttgtttt aaccaaatca tttcatctcg
     9301 tgtaagtttg tttggattaa attcaatacg catattacgt ctatcccaac tatctgcttt
     9361 cactttgtca tattcaatat aaactttttc ttgaagtgct ttagctttaa actttgtttg
     9421 aagaatatcc caaagtctta tttggggctc tacactcata aatttagaaa gggcttgagc
     9481 gttgtctcgg ttgagatttc caacgatcgt cattgcatca aaacttaatt ttggatcaga
     9541 tatgcacaca gtatgtgcgt ccaaccggct attagagtag ccggttttag aaaaattgtc
     9601 taaatcgtga ttttccaaat gatttgaatg atttgcatga tgtttttata cataaaaatc
     9661 gactccttaa tctcaatttc gtttaaggaa tcgctcaccc aaatatatat cttgatgtat
     9721 atttaaatat cgtttaatat ctaaatatac aagattataa aaacaactca gtgttttttt
     9781 ctttgaatga tgtcgttcac aaactttggt caaggcgtga gcgactcctt tttattttgt
     9841 tattaatata acactatcaa aagacttggt ctaatcagat caagtctttt tttatttaag
     9901 catttgtatt atctggtaaa cagttaaaac tactaaaaca ccaagtacat acttacttgt
     9961 tataaattct caaaggcttc tctgaatttc atttcttcaa ctccttttat tgctttattc
    10021 caatttcctt attggtcgga acctacaggg ctttatgtgt tgttgagttg gtactttctt
    10081 gggattaatc ccaattcaag tccaaccaac tcgctaacaa gttagctaac acatagccca
    10141 ttccaaccaa taagtttctc ggcataaatg catgttctat aagtgcatac ctctatctcg
    10201 tttttgattc ttttgttgtt cttgttgctg ttcctgttct gaatataatt ctggattcat
    10261 tttttatcta agtttcctgc taaaccattt agttttggtt catcttctgt taacttatta
    10321 actctttccg caaaggtatt tcttcctaaa actcgttcta actcttttag taagattttt
    10381 aaaagcttta acgctatttc tatattctct ttaagtggct ttgcattttc gtaaagttgg
    10441 ttaaaattat cgtctgcgtt ttcaatacgc tcaactgctt tatttaataa atcatctttc
    10501 tctcgtattt ccttatcttt atcatctaag gctctaccag actttatata ctcgtaatct
    10561 tccgaaatat cttgagcagc ttttatctgt ttctgaaatt cattgaaatc tttttggctt
    10621 attacaacat ttccagtttc ttgtatttct ttgctaaata aaccacctac tttttcagtt
    10681 tcttgctcat acggaacatt tataggcttt taagcgtatt taacgatttt tggtactctt
    10741 gcattaattt atcgttcttt tgctttatat ggtctgtttt ttggctctca cgttcatatt
    10801 cttgcttatg atattctgtt ttttgtttat actgacttat ttgctcatgt ttagcatttg
    10861 ttacttgtct tgattgccca cgttctaaat catatcctcg ttgtttaaca tgctcattaa
    10921 tctatcttga aacgctgtta aagctttttt attacctaca acttctttag cacttaaacg
    10981 accatcatca gttattggaa caacgccata atgcatatgt ggtgtttttt cgtccatgtg
    11041 aactgttgca tataataaat tatctttacc gtattcttgt tctaaaaact ctttagcata
    11101 ttcaaaaaac tgctttgtat cttctggcgt ttgattatca aagaaatcat tgtctgatgt
    11161 aattaaacca tcaatgtgtt taatcgcgtc tgttctaatt tttcttttgc ctgtataatt
    11221 ctgttcgatt ttttcatcaa tcaagttatt aaaattctgt tattagcatt taccaaatca
    11281 taatttaagt aagttttact atggtctata tcttcatttt cataattatt attttctctt
    11341 tgaacatgtt ttgtatgccc gttgtatttg ttccagattt aacttttgaa actctaacaa
    11401 tggaataaga catataaaaa tcactcctaa gcacaaaggt ttcatgtgta tttaatagtt
    11461 taagtctaac acactagact tatttttaat aagtcgttaa tacgtgtgct ctgcgaggct
    11521 taaacctgtt tttgctaacg caaaaaatga gtggcaaaat gctagccact catagttcta
    11581 aaccaaaata taatataact attcaaactg cttttcagaa cgtttaaata caataatcgt
    11641 caaaagacaa cataaaataa taaggatagc catggctaca agaatattac tatacactcc
    11701 agaagaataa tttataaatt ccagaactag tttacgatta atcaattgta gtgacaataa
    11761 acctcctaca attgctatac ctgttccctc tgataaaaaa cttgtgaaat ttagcaaact
    11821 cattccagaa gcaactagtt taggattaat aaaagggt
//
