LOCUS       NZ_CP065609             6888 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_946 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP065609
VERSION     NZ_CP065609.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450476
            Assembly: GCF_016026195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 6.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 644x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 16:05:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,163
            Genes (coding)                    :: 4,919
            CDSs (with protein)               :: 4,919
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 96 of 244
            Pseudo Genes (incomplete)         :: 119 of 244
            Pseudo Genes (internal stop)      :: 71 of 244
            Pseudo Genes (multiple problems)  :: 36 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065609.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_946"
                     /culture_collection="DSM:4779"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /collected_by="DSMZ"
     gene            join(6738..6888,1..269)
                     /locus_tag="I6H03_RS26105"
                     /old_locus_tag="I6H03_26115"
     CDS             join(6738..6888,1..269)
                     /locus_tag="I6H03_RS26105"
                     /old_locus_tag="I6H03_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(832..1065)
                     /locus_tag="I6H03_RS26110"
                     /old_locus_tag="I6H03_26120"
     CDS             complement(832..1065)
                     /locus_tag="I6H03_RS26110"
                     /old_locus_tag="I6H03_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1072..1590)
                     /locus_tag="I6H03_RS26115"
                     /old_locus_tag="I6H03_26125"
     CDS             complement(1072..1590)
                     /locus_tag="I6H03_RS26115"
                     /old_locus_tag="I6H03_26125"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001318237.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(<1628..2059)
                     /locus_tag="I6H03_RS26590"
                     /pseudo=""
     CDS             complement(<1628..2059)
                     /locus_tag="I6H03_RS26590"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2268..2591)
                     /locus_tag="I6H03_RS26125"
                     /old_locus_tag="I6H03_26135"
     CDS             complement(2268..2591)
                     /locus_tag="I6H03_RS26125"
                     /old_locus_tag="I6H03_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2656..2847
                     /locus_tag="I6H03_RS26130"
                     /old_locus_tag="I6H03_26140"
     CDS             2656..2847
                     /locus_tag="I6H03_RS26130"
                     /old_locus_tag="I6H03_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4057..4398
                     /locus_tag="I6H03_RS26140"
                     /old_locus_tag="I6H03_26150"
     CDS             4057..4398
                     /locus_tag="I6H03_RS26140"
                     /old_locus_tag="I6H03_26150"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(4395..5960)
                     /locus_tag="I6H03_RS26145"
                     /old_locus_tag="I6H03_26155"
     CDS             complement(4395..5960)
                     /locus_tag="I6H03_RS26145"
                     /old_locus_tag="I6H03_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            6220..6657
                     /locus_tag="I6H03_RS26150"
                     /old_locus_tag="I6H03_26160"
     CDS             6220..6657
                     /locus_tag="I6H03_RS26150"
                     /old_locus_tag="I6H03_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     Typing          complement(2933..3020)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          3031..3589
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(729..2278)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.031"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaagccga cacaacacga atgatggtgc ctttttttcg gcagttctgg gatatgggag
       61 ctaaagataa agcgacagga aaatcacgga gtgatgtgca acaacgcatt caacagtttc
      121 acagccagga atttttaaac tcactccggg gcacaactca atttgcgggt actgattacc
      181 gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc tgacacgatt tctgcggttt
      241 atctcgatgg ctacgagggc agacagtaag tggatttacc ataatccctt aattgtacgc
      301 accgccgaaa cgcgttcggt gcgatcacgg cagcagacag gtaaaaatgg caacaaacca
      361 cccggaaaac tgccgcgatc gcacccggta aattttaacc acatgcatac ctatgcagcc
      421 aggtgaatca cgcaggaatt gccagtcaga gaccgctgaa acggattttt ttcatacttt
      481 cggaagcgaa gaagacgggg atttgagcag gaaaacagaa gttcagggga atgagcgcga
      541 tctggcaata gaggcaaaaa cacgactatg aaagacacac cagaatcgcg cccgtatgcg
      601 ttttaacgcg ttcctgtgtt ttcagcaagg tttatgactg aagcgcgttc ccggtgcccc
      661 gaaccaacca ttatcccaaa aaatgcaccg gaaacgcgct gacaacattt tagcctgtct
      721 ctgattacca tcccagcgaa gggccatcca gtgtccgttc ctgtatttcc agctggcgct
      781 cacgctccag ggataactca tgttcgcgtt cctgtatcag ccgttcgtcc cttatctcct
      841 gttctcgctg tataactggc tcaagcgttc tgtccgctcg ctcaagtgct gcacctgctg
      901 actcaacttc atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat
      961 tttctgcaat tccacgaagg cgctctccca ttcgttcagc cgctgcatat agtcctgttg
     1021 cagttgctct aatgcgttca gcaaatgtct ttccagctcc gtcactctgt gtcactccat
     1081 cagttgtacc cagtccttcc cctgatgggg aatcacctct gctgatttcc cgtacggaag
     1141 gaccagaaat ttcctgttcc cgtcctgtac gaactccacg ccccatgtct tcgcgttcag
     1201 tttctgcaat gtctcttcct gcttcctgat atcttccagg ttcgcctgta tcatccctcc
     1261 aagataccag agcgtcccgc aactcacggt aaacaggaaa atgaccatcc ccagtaacat
     1321 catgcccgta ttccctgcca gctttaacac gtccttcctg tgctgcatca tcgcctcttt
     1381 caccccttcc cggtgctttt tcagtgattc ctctgtcgaa gctgtgaaca gggctatagc
     1441 gtctttgatt ttcgtctcgt ttgatgtcac agccttgctt acagattttt cgagcttgct
     1501 gaactcgtcg ttcagcattg tctctgtaga ttcggctctc tctttcagct ttttctcgaa
     1561 ctccgcgccc gtctgtaaaa gattgctcat agaatgctcc tttcagtctg atattcttcc
     1621 cgccgttcgg gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact tcaatccctg
     1681 cttccgtgag cgcctgaatc acatcctgac ggccttttat ctctcctgca tggtaaagag
     1741 catctatccc gcgtgtgacg ccctcagcaa acgcctgttt cgtttccggc aggttatcag
     1801 ggagagtcag cgtccggcgg ttctccgggg cgttcgggtc atgcagcccg taatggtgat
     1861 tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgta
     1921 ggcgttttcc ggtctccagc tccatgttcg gaatgacaaa attcagctca agccgtccct
     1981 tgtcctggtg ctccacccac aggatgctgt actgattttt ttcgagaccg ggcatcagta
     2041 cacgctcaaa gctcgccatc actttttcac gtcctcccgg cggcagctcc ttctccgcga
     2101 acgacagaac accggacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga
     2161 cttcttccgg attaccctga agcaccgttg ccccttcccg gttacgctcc cttcccagca
     2221 ggtaatcaac cggaccactg ccaccacctt ttcccctggc atgaaactta actatcatcc
     2281 cgcgccccct gttccctgac ggccagacgc aaccggcgca gctcatcccc gatggccatc
     2341 agtgcggcca ccacctgaac ccggtcgccg gaagaccact gtccgctgtt caccttacgg
     2401 gctgtctggt tcaggttatt cccgatggct gccagctgac gcagtaacgg cggtgccagt
     2461 gtcggcagcc tcccggaacg ggcaaccggc tcacccaggc agacccgccg catccatacc
     2521 gccagttgtt taccctcaca gcgttccagt aaccgggcat gttcatcatc agtaacccgt
     2581 atcgtgagca tcctctcgcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     2641 tacacggagg catcagtgac taaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     2701 agaagccaga cattaacgct gctggagaag ctcaacgaac tggacgcgga tgaacaggca
     2761 gatatctgtg aatcgcttca cgaccacgcc gatgagcttt accgcagctg cctcgcgcgc
     2821 ttcggggatg acagtgaaaa cctctgacat atgcagctcc cggagacggt cacagcttgc
     2881 ctgtgagcgg atgccgggag cagacaagcc cgccagggcg cgtcagggtg ttttagcggg
     2941 tttcggggcg cagccctgaa ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     3001 atgcagcatc agtgcggatt gtatgaaaag tgcaccatga ccagtgtgaa ataccgcaca
     3061 gatgcgtaag gagaaaatgc acgttccggc gcttttccgc ttcctcgctc actgactcgc
     3121 tacgctcggt cgttcggctg cggcgagcgg tactgactca ctcaaaaacg ataatacagt
     3181 tatccacaga atccggggat aagaccggaa agaacatgtg cgcaaaaagg caaagcccaa
     3241 caaaaggcca cgccgatggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3301 aaccgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg ttggtatctc
     3481 ggctcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3601 gcgccactgg cagcagccgt tggtaacttg gttagcagag cgatgatggc gcaaatggtg
     3661 caacagagtt cttgaagtag tggcccgact acggctacac tggaaggaca gtatttggta
     3721 tctgcgctct gctgaagcca gttacctcgg ttaagcagtt ccccaactga cttaaccttc
     3781 gatcaaaccg cctccccagg cggttttttc gtttacaggc agcagattac gcgcagaaaa
     3841 aaaggatctc aagaagatcc tttgatcttt tctactgaac agagatcccc gtcaatttag
     3901 aagaagatga tggagccaca gtccctccct gaacatcacg tatatagtta gcctgacatc
     3961 caacaaggag gtttatagcg aatattccca caaaaaatct tttcctcata actcgatcct
     4021 tataaaatga aaagaatata taacgaggtt taatttatga gtttaagata ctacataaaa
     4081 aatattttgt ttggcctata ctgcgcactt atatatatat accttataac aaaaaacaac
     4141 gaagggtatt atttcctagc gtcagataag atgctatacg caatagtgat aagcactatt
     4201 ctatgcccat attcaaaata tgctattgaa cacatatttt ttaagttcat aaagaaagat
     4261 tttttcagaa aaagaaaaaa cctaaataat gcccccgtgg caaaattaaa cctatttatg
     4321 ctatacaatc tactttgttt ggtcctagca atcccatttg gattgctagg actgtttata
     4381 tcaataaaga ataattaaat acccaacacc tcatttatag tattaagttt attcttatca
     4441 ataaaggcac atagaatgcc tgtaacaata gcaatccccc agatacctaa tgtagttcca
     4501 gcaagcacac taaaaagtaa aacaacaaca taactaactc cagcatctac agctttcttc
     4561 tctaatgtaa gaaatagcgg cttccagtca cctgtatcct taatttttag gatatcagat
     4621 accacatcat atccaaaaga aacatgtccc gtaatcttca agtacttggc aaactgatct
     4681 aaatgcttag cccagtcttc atacttaacc gcctccagtg cattgaaaat tgcatcacgg
     4741 tctgctttgc tgaatttctt atttaataca tccttgtatt tctcaaaagc agctagagct
     4801 tcattcacat tgctgatttt cttaccttta gatttatcag caagttcctg tgccattttt
     4861 gaatattttt caccatattt ttcagatagc gtttgataaa agctaactgt tgcatccaca
     4921 gcatccttaa tctgcgagtt caggagatta gtctgtgctg ttttcaaatt atcttcagca
     4981 tcatgaatac gtgaaactgc tgtacttcgc ttattattag cctgagaaat agcctcttgg
     5041 attccattta tctcagagct aatctggtta agacgcgttt ctgatgctgt tacctgcttt
     5101 tgtttttcct ccatctcatc accagctctc gcccgtagtc tggtggcctc aaaaaaagga
     5161 cgactctgaa gcggatcatt cgccctagga gataaaagtt taaccctttc atccagttct
     5221 ttatatttag cagatgcctg atccaactca tttcgttttc cggacagcgt attcgtttct
     5281 gcatcacggg catgaatact ggagcttaaa cgggtattga gcgtattaat ctcttcatcc
     5341 actttcgcca gctcagattg tgcagcagca agattttttt gtgccacctc cacagcccgg
     5401 gcctcttcac tcaatgctgc caggcgtttc tcttcatcct cagccagttt caattggcgt
     5461 tctgtttcag cctgctcctt ctctatctct ttacgtcgtt gttctgcttc ctgaaaagcc
     5521 ttttccgcag cttccgcttc tttacgggct ttttcttctg cttttgcaag gcgcaaccgc
     5581 tctgcttctg cctgcatcgc tgcattattg gcatgagcca ggtcaataac ctccggatga
     5641 gtggaattat ggcgaagcgc ctcattcaca atatccttca gatgttgagt cagcgcatcc
     5701 cggtttgctt ttgctttagc ctgtgcttct gcggcagctt ttgctcgggc agcctgttct
     5761 gcctgcgttt tcttcaattg agcagtagac catttagctg tggcatgaat ggctgcagaa
     5821 ctttcacttt tgctacctcc agttccacca ccaccgccag agccactccc gtctggatta
     5881 ccattcaaaa gagtaatgat tacctgcccc ttatcatcat aaggaacacc atctttatag
     5941 tacgctacag ctgtttccat tataaaatcc tctttgacat taaaaacaaa tagttaaaag
     6001 caatatactg tatatataat cactggtttt atatacagtt taaaattacg ccgctgcatt
     6061 ttccctgtca accctgtgga ttttcatttt tgtgaaaacg atcaaaaaaa cagccaccca
     6121 caccacgtgc tatttccacc cgatgccaca aaaaccagca caaacattac cgttctcaga
     6181 cctcattatg ttttactgaa accatgagat gagacaccta tgggacactg taactttatg
     6241 gcatggcaca cactccggga cgcactgaaa atgactggaa gatcgcgttc acagttttac
     6301 cgtgatatgc gctcaggtct tgtcagttac cgtaccagcc aggacggacg acgggagttt
     6361 gaaaccagtg aactgatccg ggcatacggc gaattaaagc ggaatgagac accagaaagg
     6421 cacactcaag gacactcaga aaaacctcat gatcagcaga cagaacgcat tctccgggaa
     6481 ctgaatgagc tgaaacaatg cctgacgctg atgcttgagg ataaacaagc ccaggatacg
     6541 gagcgccggc gccaggaagc agaacggaaa cagctacaga atgagatagc ccagctcagg
     6601 caggctctgg agcaggaaaa gaaacgggga ttctggtcca ggctgttcgg tcactgaacg
     6661 cgctcagaaa ctgatgataa gataatcttc ggataataac tcaccgagaa taaatacttt
     6721 aaggtaggga gacactcatg agacgtaccg gaaacaaact ttgtcttatc gccatgataa
     6781 cagcaacagt agctctcaca gcctgtaccc caaagggcag cgtggaacaa catacccggc
     6841 attacgtata tgcttctgat gacggttttg atcccaactt ttccaccc
//
