LOCUS       NZ_CP065608             4011 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_946 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP065608
VERSION     NZ_CP065608.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13450476
            Assembly: GCF_016026195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2020) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 6.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 644x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 16:05:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,163
            Genes (coding)                    :: 4,919
            CDSs (with protein)               :: 4,919
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 96 of 244
            Pseudo Genes (incomplete)         :: 119 of 244
            Pseudo Genes (internal stop)      :: 71 of 244
            Pseudo Genes (multiple problems)  :: 36 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065608.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_946"
                     /culture_collection="DSM:4779"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /collected_by="DSMZ"
     gene            complement(join(3093..4011,1..206))
                     /locus_tag="I6H03_RS26085"
                     /old_locus_tag="I6H03_26095"
     CDS             complement(join(3093..4011,1..206))
                     /locus_tag="I6H03_RS26085"
                     /old_locus_tag="I6H03_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640319.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097514844.1"
                     /translation="MIYRFEVEGFKGFSDKLVFDLTHRKNYEFNQDCILDGVVKKSLVY
                     GKNGIGKSNLGLALFDIVAHLTEFNVNDGMYNNYINALNNSGIARFKYDFKFGDNIVTY
                     LYGKRSVKELVFEELFINGRLVTKFNRVLDSNAYFSLSGTETLNTALAESKISIINYIK
                     NNAILNDGIEKDLFNQFIVFVEKMLYFRSLDSNNYLGLEMGRHLITPDIVERGNVDDFE
                     RFLNKAGVKCKLCVMKIQSNNGVVEDIGFDFGSKQIPFYGVASTGTKSLALFYYWLQRF
                     KDEKCVSFIFVDEFDAFYHHSLSMLIVKEMKKAGAQVIITTHNTNVMTNELLRPDCYFI
                     MDDKGITPLFEKTDKELREAHNIEKMYKAGTFND"
                     /name="ATP-binding protein"
     gene            complement(781..1104)
                     /gene="mobA"
                     /locus_tag="I6H03_RS26090"
                     /old_locus_tag="I6H03_26100"
     CDS             complement(781..1104)
                     /gene="mobA"
                     /locus_tag="I6H03_RS26090"
                     /old_locus_tag="I6H03_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1376..1594
                     /locus_tag="I6H03_RS26095"
                     /old_locus_tag="I6H03_26105"
     CDS             1376..1594
                     /locus_tag="I6H03_RS26095"
                     /old_locus_tag="I6H03_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041626.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFEAESGTRQDAERYTCA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2318..3100)
                     /locus_tag="I6H03_RS26100"
                     /old_locus_tag="I6H03_26110"
     CDS             complement(2318..3100)
                     /locus_tag="I6H03_RS26100"
                     /old_locus_tag="I6H03_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225877509.1"
                     /translation="MTERILFVFEGEKTEKTITDSLLKKFIENDKRVVLSSFKTDIYSL
                     YKLMSEDEDLDVFAVLKEKNPDLVDFDRDSFSQLFLFFDYDGHAPTASDEKIDRLLSFF
                     NEETENGKLFISYPMVESIKCISHIDAIEDFCRHTVKICDCSKFKGYVAEYAHKSLIHF
                     NLYSDEAWNDVVRMHCVKSNFIMKGNMIFPSNYFSQKDIFSMQKSKYIDPNGSVSTLSS
                     FPMLLLDFFGHQRLFVLVSGEQIEDGDVLSSEEAQRTI"
                     /name="hypothetical protein"
     Typing          complement(1114..1339)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.75"
                     /coverage="99.0"
                     /evalue="2.28e-73"
     Typing          1589..2110
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.156"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(785..1104)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.78e-64"
ORIGIN
        1 aactcagtta agtgagcgac aatatcaaaa agagcaagcc ctaagttgct tttccctata
       61 ccatttttac cgtataccaa tgattttttg acaacaccat caagtataca atcttgatta
      121 aattcataat tcttcctatg agtaagatca aacactaatt tgtctgaaaa ccccttaaac
      181 ccctcaactt caaatctgta tatcataaaa gcctcacatc aaagatgccg taactatatc
      241 atggcaccgt aaaaaaatta caccaaatat agatattgat ttttcttatc gctgcaatta
      301 aagttgcaag cgaaggctct cgcagtccta tgaaactcag catgcttgct gttttttata
      361 ggtgtcagtt cctacaactg acgaagacag gagagtacaa aatgaaacgt aaaagcgtat
      421 tcgttcgtgc ctaccgtcgc tttcgctacg gcgagtggga agacgtatgc agtcattacc
      481 gctcaatgcc tcgctaaacg acctggctga ccgcgaagag ccttcaatgc gtaaaagttt
      541 tgtaacttgc cgtgccatac cgatttacca taacgtcaca ttacgcgcac cgctaaaacg
      601 cgctgaacgc gattctgccg caaacaccgc aaaaagagat gctttccccg gggtaaaccg
      661 gaaaaatgcg tcaggatcgc tttcggctca ctgcatagct atgcatgaaa gtgaatggcg
      721 atcggtttgg gagcttacgg cgttcatacc gtctgttttc gacagtttct ctccgggaag
      781 ctaatctgcc ataagcctgg ataatagggc ccggtgatac tccgtaatag ctatcagcac
      841 ctccgcatac tctctgtccc cgacacgctt accgtcgata aagagttttt tctgcaacgc
      901 ccccagtcgc atgacttctt tcagcacccg gtcatcagtc agtgaattaa ctttcttacc
      961 gagagctgcc gcccgtaaaa aaccagaaac ggtcttgccg cattcagcag ccctttttct
     1021 gatttcctgg tcttcttctg ccgtcagacg aacagggcga ctgatagccc gcctgcgcgt
     1081 attacttcca cttcgttttg tcataaaaca tggctccgta tctgacatgg ggtgtcgggg
     1141 cgaagccctg accagggtaa ttgtaatagc gtgcatgtat gcgcggtata acaattgcac
     1201 atcctgtcct gtctgcaagt tcgaattacc aacaaagcac tgctgttttt tggcagtgac
     1261 agtgttgtct gactgaaatg tccgtcggca gaatacctgc agtaagacgg tcgaaaagtg
     1321 gtgcgcgata taatcacacg acgcagatga cagacagaac ggagaaaaca gaaagatgaa
     1381 caagcagcag caaaccgcac tcaacatggc aaaattcata aaaagccaga gcctgacgct
     1441 gctcgaaaaa ctggactcac tcgatgctga tgcacaggcc gccatgtgtg agaaactgca
     1501 cgaactcgca gaagaactcc agaacagcat acagacacgc tttgaagcgg aaagtggcac
     1561 cagacaggat gcagaacggt atacctgtgc gtaaggagaa aatgacacat aaggctctct
     1621 tccgcttcct cgctcactga ctcgctacgc tcggccgttc ggctgcggcg agcggtatca
     1681 gctcactcaa aaacggtaat gcggtgatcc acagaatcag gggataaagc caaaaggagc
     1741 atgtgagcaa aaaacgagaa ccaggaaaag gccgcgccta tggcgttttt ccataggctc
     1801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1861 ggactataaa gataccaggc gtttcccccc ggaagctccc tcgtgcgctc tcctgttccg
     1921 accctgccgt ttaccggata cctgtccgcc tttctccctt cgggaaacgt ggcgctttct
     1981 catagctcac gctgttggta tctcggcccg gtgtaggtcg ttcgctccaa gctgggctgt
     2041 gtgcacgaac cccccgttca gcccgaccgc tgcgtcttat ccggtaacta tcgtcttgag
     2101 tccaacccgg aaagacacga cttaacgcca ctggcagcag ccattggtaa ctgatttaga
     2161 ggagttagtc ttgatgttat gcgccggtta aggctaaact gaaagaacaa gttttggtga
     2221 ctgagctcct ctattctatt agccagttac ctcggttcag aaacttgccg gttactgaac
     2281 cttcgaaaaa accaccttgc cgggtggttt tttcttttca aatagtacgc tgggcttcct
     2341 ccgaactcaa tacgtctcca tcctcaattt gttcaccgct gaccagcaca aacaatcttt
     2401 ggtgcccaaa aaagtctaaa agtaacatcg ggaatgaact taatgtagat acagagccat
     2461 ttggatctat atattttgat ttctgcatac tgaatatatc tttctgtgaa aaataattag
     2521 aaggaaaaat catattcccc ttcattatga aattagattt cacacaatgc atcctaacca
     2581 catcattcca agcctcatca gaatacaaat taaagtgaat caatgactta tgagcatatt
     2641 ctgcaacata ccccttaaac ttagaacaat cacatatttt tactgtatgc ctacaaaaat
     2701 cctcaatcgc atcaatatgc gagatacatt ttatagactc aaccatagga taactgataa
     2761 ataatttacc gttttcagtt tcctcattaa aaaaacttag tagcctgtct atcttttcat
     2821 cagaagcagt tggagcatgc ccgtcataat caaagaacag aaaaagctga gagaaagaat
     2881 ctctatcaaa atctactaag tcaggatttt tttccttcaa cacggcaaat acatcaagat
     2941 cttcatcctc actcatcaac ttataaagtg aatatatatc tgtcttaaat gaagaaagaa
     3001 caaccctctt atcattctca ataaattttt ttaaaagact atcagtgata gtcttctccg
     3061 ttttctcccc ctcaaaaaca aataaaatcc gctcagtcat taaatgtacc cgctttatac
     3121 attttctcta tattatgagc ttctcgcaac tctttgtctg ttttctcgaa aagtggagta
     3181 atccctttat catccataat gaagtaacaa tcaggcctaa gcaactcatt cgtcatgaca
     3241 tttgtattat gcgttgtaat tatcacctgg gcgccggctt ttttcatctc cttaacaata
     3301 agcatggaaa gcgagtgatg ataaaaagca tcaaactcat ctacaaagat aaatgaaaca
     3361 catttttcat ctttaaatcg ttgcaaccag taataaaaaa gagctaatga ttttgtacct
     3421 gtggacgcaa ctccatagaa tggaatttgc ttactcccaa agtcaaagcc aatatcttca
     3481 acaactccat tattactttg aattttcatt acgcaaagtt tacatttaac ccctgcttta
     3541 ttcaaaaacc tttcaaaatc atcaacatta cctctctcca cgatatccgg agtgattagg
     3601 tgtcgcccca tctctagtcc aagataatta tttgaatcaa gagatctgaa atacaacatt
     3661 ttctcaacaa aaacaataaa ctgattaaat agatcctttt cgataccatc attaaggatg
     3721 gcgttatttt ttatataatt aatgatcgat attttagact ccgcaagagc agtgtttaaa
     3781 gtttctgttc ctgataatga gaaataggca tttgaatcaa gcactctatt aaatttagtt
     3841 acaagcctgc cattgataaa caactcttca aaaacaagtt ctttcacact ccttttccca
     3901 tataaataag ttacaatatt atcaccgaat ttaaaatcat acttaaatct cgcaatacca
     3961 gagttgttaa gtgcatttat atagttatta tacatgccat cattaacatt a
//
