LOCUS       NZ_CP065456             3579 bp    DNA     linear   CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain Beach Ranger plasmid pBR_03.
ACCESSION   NZ_CP065456
VERSION     NZ_CP065456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16872508
            Assembly: GCF_016067835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Huang,Y.-T.
  TITLE     Study of Klebsiella Pneumoniae Resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Huang,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) Computer Science and Information
            Engineering, National Chung Cheng University, 168, University Road,
            Chiayi 62102, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:53:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,636
            CDSs (total)                      :: 5,512
            Genes (coding)                    :: 5,403
            CDSs (with protein)               :: 5,403
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 50 of 109
            Pseudo Genes (incomplete)         :: 56 of 109
            Pseudo Genes (internal stop)      :: 12 of 109
            Pseudo Genes (multiple problems)  :: 9 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Beach Ranger"
                     /isolation_source="Taichung Veterans General Hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pBR_03"
                     /geo_loc_name="Taiwan"
                     /collection_date="2019-11-19"
     gene            complement(56..1021)
                     /locus_tag="I5M60_RS28030"
                     /old_locus_tag="I5M60_28030"
                     /pseudo=""
     CDS             complement(56..1021)
                     /locus_tag="I5M60_RS28030"
                     /old_locus_tag="I5M60_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(1056..1931)
                     /locus_tag="I5M60_RS28035"
                     /old_locus_tag="I5M60_28035"
     CDS             complement(1056..1931)
                     /locus_tag="I5M60_RS28035"
                     /old_locus_tag="I5M60_28035"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-14"
                     /protein_id="WP_001617865.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSG
                     GRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD
                     LVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETF
                     RLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAA
                     SIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLA
                     SAARIIAEGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-14"
     gene            complement(<2195..2617)
                     /locus_tag="I5M60_RS28040"
                     /old_locus_tag="I5M60_28040"
                     /pseudo=""
     CDS             complement(<2195..2617)
                     /locus_tag="I5M60_RS28040"
                     /old_locus_tag="I5M60_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            2606..3525
                     /locus_tag="I5M60_RS28045"
                     /old_locus_tag="I5M60_28045"
                     /pseudo=""
     CDS             2606..3525
                     /locus_tag="I5M60_RS28045"
                     /old_locus_tag="I5M60_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220840.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     ARG             complement(1060..1931)
                     /name="blaCTX-M-14"
                     /gene="blaCTX-M-14"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-14"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gctttgttga ataaatcaga tttcgggtaa gtctcccccg tagcgggttg tgttttcagg
       61 caatacgcac gctttcaggc atacctgctt tcgtcatttt gttcagcgct cgtaccaggg
      121 ccatagcctc cgcaacctga ccatcgtagt cacgcagcgt cagtgaaccc cgaacagctg
      181 ttttacccgg tacatcgccg tttccgctat cgagcgacgg ttgtaatctg ttgtccattt
      241 ccaccgcgca ttactcccgg tcattcgctg attagccact gcacggttac ggtctgcata
      301 ttcaccgggc cagtaacccg caccttttcg gggcgggata agcgcgctga ttttcttacg
      361 ccgcagttca tcgtgacaga gccgggtatc gtaagctcca tcggcggcgg ctgacctgat
      421 tttccggtgg gtttgccgga ttaacccggg gaaggcctct gagtccgtaa cgttgttcag
      481 cgacaggtca gcgcagatga tttcatgtgt tttactgtca actgccagat gcagcttacg
      541 ccatatacga cggcgttcct ggccatgctt tttgactttc cactcgcctt caccgaagac
      601 cttcagcccg gtggaatcaa ttaccggtgt gcgatttcac cccgggtggg cgttttgaaa
      661 ctgatattaa ccgactttgc ccgcctgctg acacagctgt aatccgggca gcgtagcgga
      721 acgttcatca gagaaaaatg gaatcaataa agccctgcgc agcgcgcagg gtcagcctga
      781 atacgcgttt aatgaccagc acagtcgtga tggcaaggtc agaatagcgc tgaggtctgc
      841 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat agcttcatca tccagccaga
      901 aagttatgga gccacggttg atgagggctt tattgtaggt gggccagttg gtgattttga
      961 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atacgtgatc tgatccttca
     1021 actcagcaaa agttcgattt attcaacaaa accagttaca gcccttcggc gatgattctc
     1081 gccgctgaag ccagcacatc gcggcggctc tctgcgttct gttgcggctg ggtaaaatag
     1141 gtcaccagaa ccagcggcgc acgaccctgc ggccagatca ccgcaatatc attggtggtg
     1201 ccgtagtcgc cgctgccggt cttatcaccc acagtccacg acgtcggtaa gccggcccga
     1261 atgctggctg cgccggtcgt attgcctttg agccacgtca ccaactgcgc ccgctgggtt
     1321 tcgcccagcg catgacccag cgtaagctga cgcaacgtct gcgccatcgc ccgcggcgtg
     1381 gtggtgtctc tcgggtcgcc gggaatggcg gtattcagcg taggttcagt gcgatccaga
     1441 cgaaacgtct catcgccgat cgcgcgggca aaagccgtca cgcctcccgg gccaccgagc
     1501 tgggcaatca atttgttcat ggcggtattg tcgctgtact gcaacgcggc cgcgctcagt
     1561 tctgccagcg tcattgtgcc gttgacgtgt ttttcggcaa tcggattgta gttaaccaga
     1621 tcggcaggct tgatctcgac aggctgatta agcagctgct tttgcgtttc actctgctta
     1681 agcaccgccg cggccgccat aactttactg gtactgcaca ttggaaagcg ttcatcaccg
     1741 cgataaagca cctgcgtatt atctgcggta tcgatgagcg cgacgcccag ccgccctccg
     1801 ctgcttttct ccagcgccgc cagcttttgc tgcaccgcac tcgtctgcgc ataaagcggc
     1861 gcgctgccca gcagcagcgg aatgcacgcc gccgccgcga acatcatccg ttgcactctc
     1921 tttgtcacca tctcaaactc ccaatacggt caatccgtgt tacatcagta ttccctaaat
     1981 tccacgtgtg ttttttatta gcttcaaaaa tcactatttc acgaagaatt tagactgctt
     2041 ctcacacatt gtaacattat ttacaaccac ctttcaatca tttttgataa atcattgatt
     2101 tcatctttgc tgcaatgata cttaataaac tctgcaagtt atccacagag caacactcaa
     2161 ttttattgat gatattctta ttataccaga cattttcata cactcccttg tacggatagt
     2221 tttccgacaa cttcatgatt acatatcttg cggttttgat tatttttgct gcaagaaata
     2281 catacttcaa acgaaaggtc tttatttgct gtctgtattc tgaagagtcc aaggaatcaa
     2341 acttgaacaa caaaaatagg ttatatgaaa gcatcatcat ttgaaacacg gcttcattcg
     2401 cccaaaatga ctttagcaag agatgaccca ccgccatgtc gtatttggct tctttgatat
     2461 agttttcagc attaccacgc ttttcatagt atataactac tttttcagaa agcaaggtag
     2521 tatttgttgg ttttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgta
     2581 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggcc
     2641 cacctacaat aaagccctca tcaaccgtgg ctccataact ttctggctgg atgatgaagc
     2701 tattcaggct ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgctattct
     2761 gaccttgcca tcacgactgt gctggtcatt aaacgcgtat tcaggctgac cctgcgcgct
     2821 gcgcagggct ttattgattc cattttttct ctgatgaacg ttccgctacg ctgcccggat
     2881 tacagctgtg tcagcaggcg ggcaaagtcg gttaatatca gtttcaaaac gcccacccag
     2941 ggtgaaatcg caccctggta attgattcca ccgggctgaa ggtcttcggt gaaggcgagt
     3001 ggaaagtcaa aaagcatggc caggaacgcc gtcgtatatg gcgtaagctg catctggcag
     3061 ttgacagtaa aacacatgaa atcatctgcg ctgacctgtc gctgaacaac gttacggact
     3121 cagaggcctt ccccgggtta atccggcaaa cccaccggaa aatcaggtca gccgccgccg
     3181 atggagctta cgatacccgg ctctgtcacg atgaactgcg gcgtaagaaa atcagcgcgc
     3241 ttatcccgcc ccgaaaaggt gcgggttact ggccagtgaa tatgcagacc gtaaccgtgc
     3301 agtggctaat cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa cagattacaa
     3361 ccgtcgctcg atagcggaaa cggcgatgta ccaggtaaaa cagctgttcg ggggttcact
     3421 gacgctgcgt gactacgatg gtcaggttgc ggaggctatg gcctggtacg agcgctgaac
     3481 aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg cctgaaaaca caacccgcta
     3541 cggggagact tacccgaaat ctgatttatt caacaaagc
//
