LOCUS       NZ_CP065452             7148 bp    DNA     linear   CON 30-NOV-2023
DEFINITION  Klebsiella pneumoniae strain Ocean Ranger plasmid pOceanRanger_03.
ACCESSION   NZ_CP065452
VERSION     NZ_CP065452.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16872509
            Assembly: GCF_015992425.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Huang,Y.-T.
  TITLE     Study of Klebsiella Pneumoniae Resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Huang,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) Computer Science and Information
            Engineering, National Chung Cheng University, 168, University Road,
            Chiayi 62102, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 22:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,036
            CDSs (total)                      :: 5,912
            Genes (coding)                    :: 5,752
            CDSs (with protein)               :: 5,752
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 81 of 160
            Pseudo Genes (incomplete)         :: 77 of 160
            Pseudo Genes (internal stop)      :: 18 of 160
            Pseudo Genes (multiple problems)  :: 15 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065452.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Ocean Ranger"
                     /isolation_source="Taichung Veterans General Hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pOceanRanger_03"
                     /geo_loc_name="Taiwan"
                     /collection_date="2019-11-19"
     gene            complement(188..>1234)
                     /locus_tag="I5M61_RS29895"
                     /old_locus_tag="I5M61_29900"
                     /pseudo=""
     CDS             complement(188..>1234)
                     /locus_tag="I5M61_RS29895"
                     /old_locus_tag="I5M61_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA];
                     GO:0007165 - signal transduction [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /name="HAMP domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1420..>2358
                     /locus_tag="I5M61_RS29900"
                     /old_locus_tag="I5M61_29905"
                     /pseudo=""
     CDS             1420..>2358
                     /locus_tag="I5M61_RS29900"
                     /old_locus_tag="I5M61_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            complement(2399..3366)
                     /locus_tag="I5M61_RS29905"
                     /old_locus_tag="I5M61_29910"
                     /pseudo=""
     CDS             complement(2399..3366)
                     /locus_tag="I5M61_RS29905"
                     /old_locus_tag="I5M61_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     gene            3310..3747
                     /locus_tag="I5M61_RS29910"
                     /old_locus_tag="I5M61_29915"
     CDS             3310..3747
                     /locus_tag="I5M61_RS29910"
                     /old_locus_tag="I5M61_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_085281207.1"
                     /translation="MLCHGTVCVVGKIRDLILQLSKSSIYSTKPTNTTLLSEKVVIYYE
                     KRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQ
                     IKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGVYEKCLV"
                     /name="transposase"
     gene            3997..4872
                     /locus_tag="I5M61_RS29915"
                     /old_locus_tag="I5M61_29920"
     CDS             3997..4872
                     /locus_tag="I5M61_RS29915"
                     /old_locus_tag="I5M61_29920"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-14"
                     /protein_id="WP_001617865.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSG
                     GRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD
                     LVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETF
                     RLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAA
                     SIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLA
                     SAARIIAEGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-14"
     gene            4907..5875
                     /locus_tag="I5M61_RS29920"
                     /old_locus_tag="I5M61_29925"
     CDS             4907..5875
                     /locus_tag="I5M61_RS29920"
                     /old_locus_tag="I5M61_29925"
                     /function="17.3 : transposon functions"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_013362812.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            <5932..7028
                     /locus_tag="I5M61_RS29925"
                     /old_locus_tag="I5M61_29930"
                     /pseudo=""
     CDS             <5932..7028
                     /locus_tag="I5M61_RS29925"
                     /old_locus_tag="I5M61_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229248.1"
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /name="TonB-dependent receptor"
     ARG             3998..4869
                     /name="blaCTX-M-14"
                     /gene="blaCTX-M-14"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-14"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gggaaccgca gaattcggaa aaaatcgtac gctaagctaa cggtgttctc gtgacagctc
       61 tttgactagg ctttctaagg ccattctgat agccctgact tcctgaaaag ccatggctaa
      121 aatttgtgcg gctaaaaggg ataaccgatg gtaaagtaag ttatccctgt cgagatactg
      181 aaaagcgtta tcctcgtttt tcccaaaact gttttgccag ttcgctcaga gcgctagtta
      241 actgagcgac agatttcgca ctttgcaaat tattctgccc ggtctgtaca ttggtatcag
      301 cagcatcgcg tatattgata atactgcggt ttatgtcttc acttactgct ccctgctgct
      361 cgaccgcagt cgctatttgc gcgttcatgt cggtaatttc gttaacgcgt tggccaattc
      421 catcaagagc tgtagctgct tcctctgcgt gagctacact cgtgtgcgct tgccgactac
      481 tttgctccat gactgtaaca gcggattgcg ctcgctcttg tagagcgctg atcatgcttt
      541 gaatatccgt tgtcgattgc tgtgtgcgag cagcaagact gcgaacctca tcggcgacaa
      601 cagcaaaacc acgcccctgc tcaccagcac gcgcggcctc aattgctgcg ttgagtgcca
      661 acaaattcgt ttgctcggcg atccctcgta taacgtcaag aacttttgat atctcgttac
      721 tttgaccttc aagctcatga ataacctgag tggcttgcct aatttcacct tcaagggcag
      781 tgattgactg gcttgtgtgg gctaccagac gctggccaga tgccgtctca gtgtctgctc
      841 ttccggccgc atctgcagca tgctgtgcat tgctcgcaac ctcttgaatg cttgccacca
      901 tttggtttac tgccgttgct atttgatctg tctctgcctg ctgctcaact gtaagtacat
      961 tgcttgactc aatatccttt agtaggcctc gggtgtgttc gctaagccga tttgatgcat
     1021 cacctatgcg acctactatg gcgcctgttt cagcttgcat cattcgtaaa gcaaactcta
     1081 tttggccaaa ctcatcggtg cgcccagtgt agagggattg acttaatggg ttattggaaa
     1141 tattcctggc tctttcaacc agtcttccaa gaggagagag aatagccaaa acactaacag
     1201 agcttaagct tcctgacatt aaagtggcta acacctagat tctacgtcag tacttcaaaa
     1261 agcataatca aagccttgat aaatatgcat tccttcgaaa ttcagctttc acccattggg
     1321 tgaaagaaaa gtgctcaaaa atatgttaaa ttatcagctt ttatgactcg atatatggta
     1381 aaataatagt aagaaaagta gtaaaaaggg gttctaatta tgattaataa aattgatttc
     1441 aaagctaaga atctaacatc aaatgcaggt ctttttctgc tccttgagaa tgcaaaaagc
     1501 aatgggattt ttgattttat tgaaaatgac ctcgtatttg ataatgactc aacaaataaa
     1561 atcaagatga atcatataaa gaccatgctc tgcggtcact tcattggcat tgataagtta
     1621 gaacgtctaa agctacttca aaatgatccc ctcgtcaacg agtttgatat ttccgtaaaa
     1681 gaacctgaaa cagtgtcacg gtttctagga aacttcaact tcaagacaac ccaaatgttt
     1741 agagacatta attttaaagt ctttaaaaaa ctgctcacta aaagtaaatt gacatccatt
     1801 acgattgata ttgatagtag tgtaattaac gtagaaggtc atcaagaagg tgcgtcaaaa
     1861 ggatataatc ctaagaaact gggaaaccga tgctacaata tccaatttgc attttgcgac
     1921 gaattaaaag catatgttac cggatttgta agaagtggca atacttacac tgcaaacggt
     1981 gctgcggaaa tgatcaaaga aattgttgct aacatcaaat cagacgattt agaaatttta
     2041 tttcgaatgg atagtggcta ctttgatgaa aaaattatcg aaacgataga atctcttgga
     2101 tgcaaatatt taattaaagc caaaagttat tctacactca cctcacaagc aacgaattca
     2161 tcaattgtat tcgttaaagg agaagaaggt agagaaacta cagaactgta tacaaaatta
     2221 gttaaatggg aaaaagacag aagatttgtc gtatctcgcg tactgaaacc agaaaaagaa
     2281 agagcacaat tatcactttt agaaggttcc gaatacgact actttttctt tgtaacaaat
     2341 acggctttgt tgaataaatc agatttcggg taagtctccc ccgtagcggg ttgtgttttc
     2401 aggcaatacg cacgctttca ggcatacctg ctttcgtcat tttgttcagc gctcgtacca
     2461 gggccatagc ctccgcaacc tgaccatcgt agtcacgcag cgtcagtgaa cccccgaaca
     2521 gctgttttac ccggtacatc gccgtttccg ctatcgagcg acggttgtaa tctgttgtcc
     2581 atttccaccg cgcattactc ccggtcattc gctgattagc cactgcacgg ttacggtctg
     2641 catattcacc gggccagtaa cccgcacctt ttcggggcgg gataagcgcg ctgattttct
     2701 tacgccgcag ttcatcgtga cagagccggg tatcgtaagc tccatcggcg gcggctgacc
     2761 tgattttccg gtgggtttgc cggattaacc cggggaaggc ctctgagtcc gtaacgttgt
     2821 tcagcgacag gtcagcgcag atgatttcat gtgttttact gtcaactgcc agatgcagct
     2881 tacgccatat acgacggcgt tcctggccat gctttttgac tttccactcg ccttcaccga
     2941 agaccttcag cccggtggaa tcaattacgt gtgtgcgatt tcaccccggg tgggcgtttt
     3001 gaaactgata ttaaccgact ttgcccgcct gctgacacag ctgtaatccg ggcagcgtag
     3061 cggaacgttc atcagagaaa aaatggaatc aataaagccc tgcgcagcgc gcagggtcag
     3121 cctgaatacg cgtttaatga ccagcacagt cgtgatggca aggtcagaat agcgctgagg
     3181 tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatagctt catcatccag
     3241 ccagaaagtt atggagccac ggttgatgag ggctttattg taggtgggcc agttggtgat
     3301 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatacg tgatctgatc
     3361 cttcaactca gcaaaagttc gatttattca acaaaaccaa caaatactac cttgctttct
     3421 gaaaaagtag ttatatacta tgaaaagcgt ggtaatgctg aaaactatat caaagaagcc
     3481 aaatacgaca tggcggtggg tcatctcttg ctaaagtcat tttgggcgaa tgaagccgtg
     3541 tttcaaatga tgatgctttc atataaccta tttttgttgt tcaagtttga ttccttggac
     3601 tcttcagaat acagacagca aataaagacc tttcgtttga agtatgtatt tcttgcagca
     3661 aaaataatca aaaccgcaag atatgtaatc atgaagttgt cggaaaacta tccgtacaag
     3721 ggagtgtatg aaaaatgtct ggtataataa gaatatcatc aataaaattg agtgttgctc
     3781 tgtggataac ttgcagagtt tattaagtat cattgcagca aagatgaaat caatgattta
     3841 tcaaaaatga ttgaaaggtg gttgtaaata atgttacaat gtgtgagaag cagtctaaat
     3901 tcttcgtgaa atagtgattt ttgaagctaa taaaaaacac acgtggaatt tagggaatac
     3961 tgatgtaaca cggattgacc gtattgggag tttgagatgg tgacaaagag agtgcaacgg
     4021 atgatgttcg cggcggcggc gtgcattccg ctgctgctgg gcagcgcgcc gctttatgcg
     4081 cagacgagtg cggtgcagca aaagctggcg gcgctggaga aaagcagcgg agggcggctg
     4141 ggcgtcgcgc tcatcgatac cgcagataat acgcaggtgc tttatcgcgg tgatgaacgc
     4201 tttccaatgt gcagtaccag taaagttatg gcggccgcgg cggtgcttaa gcagagtgaa
     4261 acgcaaaagc agctgcttaa tcagcctgtc gagatcaagc ctgccgatct ggttaactac
     4321 aatccgattg ccgaaaaaca cgtcaacggc acaatgacgc tggcagaact gagcgcggcc
     4381 gcgttgcagt acagcgacaa taccgccatg aacaaattga ttgcccagct cggtggcccg
     4441 ggaggcgtga cggcttttgc ccgcgcgatc ggcgatgaga cgtttcgtct ggatcgcact
     4501 gaacctacgc tgaataccgc cattcccggc gacccgagag acaccaccac gccgcgggcg
     4561 atggcgcaga cgttgcgtca gcttacgctg ggtcatgcgc tgggcgaaac ccagcgggcg
     4621 cagttggtga cgtggctcaa aggcaatacg accggcgcag ccagcattcg ggccggctta
     4681 ccgacgtcgt ggactgtggg tgataagacc ggcagcggcg actacggcac caccaatgat
     4741 attgcggtga tctggccgca gggtcgtgcg ccgctggttc tggtgaccta ttttacccag
     4801 ccgcaacaga acgcagagag ccgccgcgat gtgctggctt cagcggcgag aatcatcgcc
     4861 gaagggctgt aactggtttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     4921 cacgtatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     4981 ctggcccacc tacaataaag ccctcatcaa ccgtggctcc ataactttct ggctggatga
     5041 tgaagctatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg
     5101 ctattctgac cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct
     5161 gcgcgctgcg cagggcttta ttgattccat tttttctctg atgaacgttc cgctacgctg
     5221 cccggattac agctgtgtca gcaggcgggc aaagtcggtt aatatcagtt tcaaaacgcc
     5281 cacccggggt gaaatcgcac acctggtaat tgattccacc gggctgaagg tcttcggtga
     5341 aggcgagtgg aaagtcaaaa agcatggcca ggaacgccgt cgtatatggc gtaagctgca
     5401 tctggcagtt gacagtaaaa cacatgaaat catctgcgct gacctgtcgc tgaacaacgt
     5461 tacggactca gaggccttcc ccgggttaat ccggcaaacc caccggaaaa tcaggtcagc
     5521 cgccgccgat ggagcttacg atacccggct ctgtcacgat gaactgcggc gtaagaaaat
     5581 cagcgcgctt atcccgcccc gaaaaggtgc gggttactgg cccggtgaat atgcagaccg
     5641 taaccgtgca gtggctaatc agcgaatgac cgggagtaat gcgcggtgga aatggacaac
     5701 agattacaac cgtcgctcga tagcggaaac ggcgatgtac cgggtaaaac agctgttcgg
     5761 gggttcactg acgctgcgtg actacgatgg tcaggttgcg gaggctatgg ccctggtacg
     5821 agcgctgaac aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg cctgaaaaca
     5881 caacccgcta cgggggagac ttacccgaaa tctgatttat tcaacaaagc cacgcgctgg
     5941 acacaccacg atgacagcgg ttttatccct tacgccatca ccaccggcct tgattacgag
     6001 gcgatgaccg aacagcgcaa aggctatgag aactttgttt atcagaacgg cgtttacgat
     6061 ctcggcacca aaggtgatat gcgacgtaac gaacgtaacc tgatgtggaa cctcgatccc
     6121 tacattcaaa cgcactggca gctcacctca gccctggggc tggacgccgg ggcgcgctat
     6181 agctcggtat ggtttgattc caacgatcgt tatgttgtag gtaaaaatgg tgatgatagc
     6241 ggcgaagcga gctatcacaa gtggcttccg gcggcagcgc tgaagtatgc catgacgccg
     6301 cagtggaacg tttaccctct gccgggaggg gatttgaaac gccgaccatt aacgagctgt
     6361 cctatcgtcc cgatggtcag tccgggctta actttgctct gcaaccggcc actaacacca
     6421 ccgttgaagc aggcagtaaa tggcaggtgg gcaccgggat ggcgagcctg agcgtcttca
     6481 atacggacac gaaaaatgaa attgtcgttg ccgccagcga cagtggccgt acgtcctatc
     6541 agaacgcggg cgagacgcgc cgccgcgggg tcgaactggg atgggatcag caatttgcgc
     6601 ccgcctggcg cgccaaactg gcgtggacgc tgctggatgc gacctaccgt gaaaacgccg
     6661 gcgatgctat tcagagcggc aatcggatcc cggggattgc acgtaatagc gcctatgcct
     6721 cctttggctg ggtgccggaa gagggatggt acgctggcgc tgaagtccgc tatatgagcg
     6781 atattcaggt caacgatgcc aatagcgagc aggctccagc ctatacggtg acggcactga
     6841 ataccggcta taaatatgtg ctagataact ggaccgtcga cctctatacg cgcgttgata
     6901 acctgtttga taagcgctac gtcggctctg ttatcgtcaa cgagagcaac ggacgctatt
     6961 atgaaccggc gccgggccgt aactatggcg ttgggctatc ggtcagctat cagtttgatt
     7021 cgctgtaaca atacggctgc cggagagtgc tattgaggtg aggctccggc agcgggtaaa
     7081 ggcgttgatt ccctggtaac aataagctgc ttattgatgt atcagtaagc atgccggcag
     7141 ccattgcg
//
