LOCUS       NZ_CP065416             2953 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Pseudomonas aeruginosa isolate P33 plasmid pP33-4, complete
            sequence.
ACCESSION   NZ_CP065416
VERSION     NZ_CP065416.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16965915
            Assembly: GCF_015832055.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Cai,H.
  TITLE     Genetic characterization of a novel blaKPC-2 carrying plasmid in
            Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Cai,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2020) Sir Run Run Shaw Hospital, Sir Run Run Shaw
            Hospital, 3 East Qingchun Rd, Hangzhou, Zhejiang 300016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 02:28:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,696
            CDSs (total)                      :: 6,616
            Genes (coding)                    :: 6,541
            CDSs (with protein)               :: 6,541
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 53 of 75
            Pseudo Genes (internal stop)      :: 4 of 75
            Pseudo Genes (multiple problems)  :: 11 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065416.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /isolate="P33"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pP33-4"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019-08-27"
     gene            join(2712..2953,1..733)
                     /gene="mobV"
                     /locus_tag="I5U70_RS33370"
                     /old_locus_tag="I5U70_33380"
     CDS             join(2712..2953,1..733)
                     /gene="mobV"
                     /locus_tag="I5U70_RS33370"
                     /old_locus_tag="I5U70_33380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(760..1110)
                     /locus_tag="I5U70_RS33375"
                     /old_locus_tag="I5U70_33385"
     CDS             complement(760..1110)
                     /locus_tag="I5U70_RS33375"
                     /old_locus_tag="I5U70_33385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(1107..1658)
                     /locus_tag="I5U70_RS33380"
                     /old_locus_tag="I5U70_33390"
     CDS             complement(1107..1658)
                     /locus_tag="I5U70_RS33380"
                     /old_locus_tag="I5U70_33390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     Typing          2704..2951
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.723"
                     /coverage="83.0"
                     /evalue="1.4299999999999992e-34"
ORIGIN
        1 ggaatggtgg gccaaggcca cgccccagca gcaggccgcg ttcttcgacc aggcgcaggg
       61 ctggctggcg gccaaatacg gcgcggatcg catcgtgacg gccagcatcc accgcgacga
      121 ggccaccccg cacctgtcgg ccttcgtcgt gccgctgacg caggacgggc ggctgtcggc
      181 caaggagttc atcggtggcc gcgacaagat gcgggccgac cagaccagct ttgccgaggc
      241 ggtgagggat ctcgggctgg aacgcggcat cgagggcagc cgggccaccc atcagcgcgt
      301 ccagagctac tacggcgcca tcgagcgcca gcccgggcac gccaccatca cgccgcaggc
      361 catcgagccg cgggtgctgc gcaaggggat tttctcgaag gacgtggaga cgccggaggc
      421 cgtggccgcc cgcgtgaccg ctgccgtcag ggaagggtat ggcccgaccg tcgcagcggc
      481 cgcaggagcg cgtcaggagc gcgagaaggc ccggcaggca caggagacag cccggagcct
      541 gcgtgaccgc ctgaagcccg ttctggacgc tctggggccg cttaatcgcg atatgcaggc
      601 caaggcggcc cagatcatca aggccgtggg cgagaagctg ctcgccgaac agcgcgaggc
      661 gacacgccag cggcaggccc aacggcttcg cagtcgcggc cgggatcgcg acgacggcgg
      721 gctgtctcgt tgaccctaca ggtggccgac ggaaggggtt tatggcttgc agctactgag
      781 gacggtttgc tccttcccgc catcgtgggt cgaaaggttc atgtcgttgc ccttacccca
      841 cagttcgtag gtgtagtttt tgctagcgga gatgtagcgc actcctgatg cgctcttggc
      901 gcgtttcagt gggacaagct catccatttg cagcatcacg gcatagtctc cgacgtacac
      961 gacatccaac gtctttccgc cttcgcaagc gtacttggtc tggatcatct catccgctgc
     1021 tgaagcaaga ttcggagcga ttgccgccat catggcagca agagcgaacg tttttttcat
     1081 aaagattttc tccttcgttt cgttgctcat tggggagtgc tttcaaacat gtcaatggtg
     1141 ttcgggtcgc ggtcgtcgtt ctgcttctcg ccgctgaaca tcgacatgat ccggcggcgg
     1201 atatcgccat ccttgttgac caaggttttg gtcacgaaag ctgcgtaatc cttggcgttg
     1261 tcgtagccct tccagacctc gtgaggatcc agcgcatagg cgcaaacctt gccagcaaca
     1321 gggatgcgga caagcgcgcg attttcttcc aggtaagaca accaacggct gattgtccgc
     1381 ctgttgacgt tcatcttgtc tgccaaaaac tgctgatcgc agaccaccgc gccgcaggtt
     1441 gggtcgatat tttcagcaaa gaatgaatac aggccgaagg ccccagcatt gcctttagca
     1501 agctcaatga tgcgcttcca acccaaggga tacacctgcg tgaacccctt gttcttccgc
     1561 gcctcttcgc gctcctgagc ctctttctcg tccagcatgg ccagctctcg ctggcggata
     1621 cgacgctggg cgtcggtcgc caccgtgatc tgctgcatgg ggttcctcca aagtcgggaa
     1681 atttgtccgc tcattgcgcc atttcgagtc gcaatgatac gacaaattat gtctcacgtc
     1741 gagacagttt gcgtctcaac cttgctacct gttgagacat ccatgcatgt ctcaacgtta
     1801 ggacacccta tgtgtctcaa tgcatttcta caaatcctta taaatcaata acttagcatt
     1861 tccccctttc tcctctaagg ggctgccgcc ccgccggcgg ccgcgtagcg gccttggcac
     1921 cccgctgacg gcccctgcgg ggcctctgga aaaggcccac ccggccaccg gccgggtgac
     1981 gttgtttggg gggcctccgg cggccgctgc gcagccttgg ggcggtgggc gctgcccccc
     2041 gccacccccc gcgttcgcca cgtggcaggg gtgcaggggc tacgggcctg cggcccttca
     2101 ctcctgcacc cctaccgggg agcgtggggt cgttccaggc gcgtcaaggg ccgctgcgct
     2161 ctccgggggt ggcccgtgtc ctcgccctgc gggctgcggg ccgcgccagc caccccctgc
     2221 gcccttgacc cgccttccac tccggcgctg tcggaaattt cctacccggc catgaggcga
     2281 tttccgacac gccagcgggc cactggctga cgccttcagc ggagggcagg gtcaagggcg
     2341 cgcccgcagg ccgcagcccg gagcgaagcg cagggaccgg gcgaggacgc gggcgaagcc
     2401 gaacccttga cgctgaccgc agcgccccag cctgtgcgag tgggggcagg gacgaagggc
     2461 gaagcccgcc acccctgccc cccgagcctc ccggcggcga gggtggggag tccagagggg
     2521 caaagcccct ttgggcaagg ccgaaggccg ccgcaggagc aagcccgaag ggcgctgcaa
     2581 ccgccgcagg cgggcacgac tttgcttcgc aaagtctagt gagtacacta tcatctgata
     2641 gtggcccgcc ggaggcaccg ccttgcgctg ccccgactgg ccggattcgg acaccaaggg
     2701 gaggggcagg catggcggcc tacgcgatca tgcgatgcaa gaagctggcg acgatgggca
     2761 gcgtggcggc ggcgttgcag cactgctacc gcgagcgcga aacgcccaac gctgacgccg
     2821 aacgcacgcc ggacaacgag caccgggcgg ccacctcaac cgatgcggcg atggggcggc
     2881 tgcgcgagtt gctgccggag aagcggcgca aggacgcggt gctggcggtc gaatacgtga
     2941 tgacggccag ccc
//
