LOCUS       NZ_CP065415             3014 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Pseudomonas aeruginosa isolate P33 plasmid pP33-3, complete
            sequence.
ACCESSION   NZ_CP065415
VERSION     NZ_CP065415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16965915
            Assembly: GCF_015832055.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Cai,H.
  TITLE     Genetic characterization of a novel blaKPC-2 carrying plasmid in
            Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Cai,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2020) Sir Run Run Shaw Hospital, Sir Run Run Shaw
            Hospital, 3 East Qingchun Rd, Hangzhou, Zhejiang 300016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 02:28:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,696
            CDSs (total)                      :: 6,616
            Genes (coding)                    :: 6,541
            CDSs (with protein)               :: 6,541
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 53 of 75
            Pseudo Genes (internal stop)      :: 4 of 75
            Pseudo Genes (multiple problems)  :: 11 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /isolate="P33"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pP33-3"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019-08-27"
     gene            complement(289..600)
                     /locus_tag="I5U70_RS33355"
                     /old_locus_tag="I5U70_33365"
     CDS             complement(289..600)
                     /locus_tag="I5U70_RS33355"
                     /old_locus_tag="I5U70_33365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079391879.1"
                     /translation="MSVNTVENVEPVGKYQRSVALEKPMADRLHKVCEHLGVTVGAYLK
                     QAIGESVCRHEMTLFPKQQGQDMQAMLAKFFEEVAEAAGDELNAQAERPKRQRAVKAK"
                     /name="hypothetical protein"
     gene            complement(1358..1630)
                     /locus_tag="I5U70_RS33360"
                     /old_locus_tag="I5U70_33370"
     CDS             complement(1358..1630)
                     /locus_tag="I5U70_RS33360"
                     /old_locus_tag="I5U70_33370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043501020.1"
                     /translation="MAKPYPIVPLSWLDGFNDLNDQLVAYELAVRALFRMFAEDGSPYD
                     LETVLSGLHDLVQPVIEGYQAIAEQCRTAESMGMVGVAQLADDKE"
                     /name="hypothetical protein"
     gene            complement(1711..2766)
                     /locus_tag="I5U70_RS33365"
                     /old_locus_tag="I5U70_33375"
     CDS             complement(1711..2766)
                     /locus_tag="I5U70_RS33365"
                     /old_locus_tag="I5U70_33375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279028.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_231405956.1"
                     /translation="MGRRVNVFKPEEQFPGNVYRTFDCRHVRTAHFVGLHYRPEYLQAH
                     YRGIATCGSVWACPVCASVIQERRRLELEHLVQWAEDQGLQAIMVTFTFPHTGFDTLSE
                     LLDRQADAFKRFRTGGPFRRLKAALGFEGLVRSLEVTHGLRNGWHPHTHELWLVKSSVD
                     LATCSRISELWERACLASGLLQHDDQVKVEAFRKHSVDVRLNATSGEYLAKQDSSRKWG
                     ITHEVAKATSKAGKSKGVHPHEFLVRKAPGDEARYFEYLEAMKGRRQLFWSPGLKGRCG
                     LDEVDDQVIAEEASDAADLLGHLVQEEWDFIRSKRVVAQVLDAAEVGGWELVMRYLKRL
                     GFRDRPPPEFP"
                     /name="protein rep"
     Typing          complement(1711..2766)
                     /name="rep_cluster_836"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.342"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaagcccc cgattgccgc caagcctagg gggctttttc tttggtgcgg ttatcgccta
       61 accaaccgac cgtcctcgac atcaactcca ggaaaaccct ggagcaacgt cgcacacaat
      121 tgaagagcag cagcacctgc atctccatcg accccgcgaa gcgacagata ggtagcgcca
      181 cactgaccgc aataccccgt cactagcatc gccacaggct caccccacgg aacagcatga
      241 gcaacatgcc aatggccgca ggtagaacac tgatgcttga tcctcagatc acttggcctt
      301 aaccgcacgc tgacgcttgg gccgttctgc ttgggcattc aattcgtccc cggccgcctc
      361 ggcgacctct tcaaaaaact tcgcgagcat ggcctgcatg tcttgtccct gctgcttggg
      421 gaacagagtc atttcgtgcc ggcaaacaga ctcaccaatg gcttgtttca gataagcccc
      481 tacggtgacg cccaaatgct cacagacctt gtgcaaccga tccgccatag gcttttccaa
      541 agcaaccgat cgctgatact tacctacggg ctctacgttc tctaccgtgt ttacgctcat
      601 tctctagttc tcataccggt tcatatcaac cacccgtgaa ccaaacatat ccgacagtca
      661 gtgaaaagca caacaacttc catcaaagag cgcccctgga gcacgcccag ggacaaaacg
      721 gggccacctc gaagacaatc cagagacctc ctccttcgct ccggaacgat cgctccgtcg
      781 tcggccgatc tgatttcaga ttcttaaact cttccaggtg gtgttttccc acctgcgcgc
      841 cctcggtgag aaatcaccac ctggtcagca ccggattggt actaaatcat cgtcctgcag
      901 ggagaccagg tagagcgaac tggtaccacg gcagcgctgc tcgctgggct agacggcgga
      961 taacccctcg gaagtctgca tgccaggcgg ctgcatccac tcaacgccat ctgtaagccc
     1021 gcagaacgcc cctatgaggt gatcaggcgc gaaggtggcc cggagtggcc agaaatgaaa
     1081 aaggcccgcg aaggagcctc tgaggggctt ctaggcccat tgagcaaacg ggcggcgtct
     1141 cgctcaagac gagacgctcg cgccgccctc gaggagggga gggggttcca gggtctccct
     1201 ggacgcatcg accgtaaacg gtcggtcacg tgcctacgca tctcaacgaa ttatcgtgac
     1261 gtaacggaaa atgcccgtcg ggtgcggcac ggtggtgctc gcatgctcgc atccatccgt
     1321 gtcttacccg acgggcatta atcgtgacgc gtaacggtta ttccttgtcg tcggccagct
     1381 gggccacgcc aaccatgccc atcgattcag cggtgcggca ctgttcagca atcgcctggt
     1441 aaccctcgat caccggctgc accaggtcat gcaggcccga cagcaccgtc tccaggtcgt
     1501 agggcgatcc atcctcggcg aacatccgaa acagcgcccg gacggccagt tcgtaggcga
     1561 ccaattggtc gttgaggtcg ttgaaaccgt ccagccagga cagcgggacg ataggatatg
     1621 gcttagccat ggtgttctcc ttctcagagc atcgtggtta gccgggcggg tggattggcc
     1681 tccagcctgc ccggcgtctt ttccgtgacg tcacggaaat tcgggcgggg gtctatccct
     1741 gaacccaagg cgtttcaggt agcgcatgac cagctcccag ccgcctacct cggcagcatc
     1801 cagaacctgg gcgacaacgc gcttcgagcg gatgaaatcc cactcctcct gcaccaggtg
     1861 accgagcaag tcagccgcat cagaggcctc ttcggcaatc acctggtcgt ctacttcgtc
     1921 gaggccacaa cgccccttca ggccgggcga ccagaacagc tggcgacgcc ctttcatggc
     1981 ctccagatac tcgaaataac gggcctcatc accaggggcc ttgcgcacca ggaactcatg
     2041 cggatgcacg cccttggact tgccagcctt gctggtggcc ttggcaacct catgggtgat
     2101 gccccacttc ctggaggagt cttgcttggc caggtactca ccagaggtgg cgttcaagcg
     2161 gacgtccacc gagtgcttgc ggaaggcctc gaccttcacc tgatcgtcat gctgcaggag
     2221 gcccgaagcg aggcacgcgc gctcccacag ttcggagatc ctggagcagg tggcgagatc
     2281 caccgaggac ttcaccagcc agagctcatg ggtgtgcggg tgccatccgt tgcggaggcc
     2341 gtgggtgacc tccagggagc gcaccaggcc ctcgaagcct agggctgcct tcaggcgccg
     2401 gaaggggcca ccggtgcgga agcgcttgaa ggcgtcggcc tggcggtcga gcaactcgga
     2461 cagggtgtcg aagccggtat gcgggaaggt gaacgtcacc atgatcgctt gcagaccctg
     2521 atcctcggcc cactgcacca ggtgctcgag ctcaaggcgt cggcgctcct ggatcacgct
     2581 cgcacacacc gggcaggccc atacggagcc acaggtggcg atcccacggt aatgcgcctg
     2641 caggtactca ggccggtagt ggaggcccac gaagtgcgcc gtgcgcacgt ggcgacagtc
     2701 gaaggtgcgg tagacgttgc ctgggaactg ctcctcaggc ttgaacacgt tgacccggcg
     2761 acccatccag gaacgggcct gagcgagcgc ctgataccgc tcctggcgag cagaacgagc
     2821 accctgatcg cagccagggg gggtcgagga tttcgcagtt ataccaagag gagccccgcg
     2881 ccccccctca gatgccagtc tagagccgct ctgaggcgat gtttgacagc gcccctcaag
     2941 ggcatctgct ggcccgtata cgggctcttc gagtttgcta tgatgcacaa agtgtcctat
     3001 gcggatgcac cctc
//
