LOCUS       NZ_CP065198             2362 bp    DNA     circular CON 09-FEB-2024
DEFINITION  Staphylococcus aureus strain GDC6P096P plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP065198
VERSION     NZ_CP065198.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16871896
            Assembly: GCF_015767495.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ji,X.
  TITLE     Comparative Analysis of Genome, Fitness and Virulence of MRSA ST398
            and ST9 Isolated in Both China and Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Ji,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2020) College of Veterinary Medicine, China
            Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/09/2024 05:25:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 40 of 86
            Pseudo Genes (incomplete)         :: 39 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 18 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065198.1.
            Bacteria and source DNA available from Veterinary Pharmacology and
            Toxicology Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="GDC6P096P"
                     /isolation_source="pig farms"
                     /host="pig"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Guangdong"
                     /lat_lon="30.939972 N 121.33651 E"
                     /collection_date="2016-05-28"
                     /collected_by="Xing Ji"
     gene            complement(336..1070)
                     /gene="erm(C)"
                     /locus_tag="I3K80_RS14215"
                     /old_locus_tag="I3K80_14215"
     ARG             complement(340..1070)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1774..2250)
                     /locus_tag="I3K80_RS14220"
                     /old_locus_tag="I3K80_14220"
     CDS             complement(1774..2250)
                     /locus_tag="I3K80_RS14220"
                     /old_locus_tag="I3K80_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             complement(337..1070)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(1765..2250)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaattcctt ataaacctta tcatctcaac ctatattaag attttaccta gttgaatctt
       61 cttttctata taaagcgtcg gagcatatca gggggttatc taacgtaaat gctacccttc
      121 ggctcgcttt cgctcggcat tgacgtcaga tactgcaccc cctgaacccc catgctccaa
      181 cagcaaaaag gaaacttttt gctgcttttc cgacgcttat tcgcttcgct catatttata
      241 tagaaaagaa gtgaatgcgc aaaagacata atcgattcac aaaaaatagg tacacgaaaa
      301 acaagttaag ggatgcataa actgcatccc ttaacttact tattaaataa tttatagcta
      361 ttgaaaagag ataagaattg ttcaaagcta atattgttta aatcgtcaat tcctgcatgt
      421 tttaaggaat tgttaaattg attttttgta aatattttct tgtattcttt gttaacccat
      481 ttcataacga aataattata cttctgttta tctttgtgtg atattcttga tttttttcta
      541 tttaatctga taagtgagct attcacttta ggtttaggat gaaaatattc tcttggaacc
      601 atacttaata tagaaatatc aacttctgcc attaaaaata atgccaatga gcgttttgta
      661 tttaataatc ttttagcaaa cccgtattcc acgattaaat aaatctcatc agctatacta
      721 tcaaaaacaa ttttgcgtat tatatccgta cttatgttat aaggtatatt accaaatatt
      781 ttataggatt ggtttttagg aaatttaaac tgcaatatat ccttgtttaa aacttggaaa
      841 ttatcgtgat caacaagttt attttctgta gttttgcata atttatggtc tatttcaatg
      901 gcagttacga aattacacct ctgtactaat tcaagggtaa aatgcccttt tcctgagccg
      961 atttcaaaga tattatcatg ttcatttaat cttatatttg tcattatttt atctatatta
     1021 tgttttgaag taataaagtt ttgactgtgt tttatatttt tctcgttcat tataaccctc
     1081 ttttttcctc cttataaaat tagtataatt atagcacgag ctctgataaa tatgaacatg
     1141 atgagtgatc gttaaattta tactgcaatc tgatgcgatt attgaataaa agatatgaga
     1201 gatttatcta gtttcttttt ttacaagaaa aaagaaagtt cttaaaggtt ttatactttt
     1261 ggtcgtagag cacacggttt aacgacttaa ttacgaagta aataagtcta gtgtgttaga
     1321 ctttaatgtt tttttaaggc attagtgcat ttaagcgtca gagcatggct ttatgccgag
     1381 aaaactattg gttggaatgg cgtgtgtgtt agccaaagct ttggcgagtt ggttgggggt
     1441 ttcatgggat taatcccatg aaagtaccaa ctcaacaaca cactaacgcc tgttggttcc
     1501 aaccaatagg aaattggaat aagcaattag tataatgaga gtataatgtt ggtataacgt
     1561 tagtataatg atgctttttt tcattatatt ttttatgtac tttaaacctg cacgcttatg
     1621 tgaattagaa aaagcttaat cgcatttcat agattgacct cccaataact acgtggtgtt
     1681 attgggaggt caatctattt catttgcgtt aagcttttga tctacattaa gcgtgcaggt
     1741 tttttgtcta tttgtagacg attttatgtt atactagtcc ttgaaagaat aataatcaga
     1801 taatgcattt tcttgttttt catttgcctc ttgctcaaag ttcccaaatt cgagtaagag
     1861 gtatttttgt ttttggtcgt cgcctctcat tagtagttca gggtttaaca ttaatactcc
     1921 agtttttctt tttataatat ttccttcttc taagatttta agtgttgtta ttactgtttg
     1981 tagacttgtt cctgtagctt ttgctatttc tcttgttgta gctatcattg tattgttact
     2041 taagtggaca ttatctagga tatagttaac gattttaagt ttttttccgc caatcatatc
     2101 taacatactt attaattgca ctatatatgc ctttacgaag ttaccagacg tttgtttacg
     2161 gtataacttg tctacctcta tgacttctcc actttcttcg tctatgagcc tctgagagcc
     2221 tttatagact gttccatatc tttctttcat ctttttctca ctccttattt taaactattc
     2281 taactatatc ataactgttc taaaaaaaaa agaacatttg ttaaaagaaa ttagaacaaa
     2341 atgagtgaaa aattagaaca aa
//
