LOCUS       NZ_CP065197             2990 bp    DNA     circular CON 09-FEB-2024
DEFINITION  Staphylococcus aureus strain GDC6P096P plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP065197
VERSION     NZ_CP065197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16871896
            Assembly: GCF_015767495.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ji,X.
  TITLE     Comparative Analysis of Genome, Fitness and Virulence of MRSA ST398
            and ST9 Isolated in Both China and Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Ji,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2020) College of Veterinary Medicine, China
            Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/09/2024 05:25:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 40 of 86
            Pseudo Genes (incomplete)         :: 39 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 18 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065197.1.
            Bacteria and source DNA available from Veterinary Pharmacology and
            Toxicology Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="GDC6P096P"
                     /isolation_source="pig farms"
                     /host="pig"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Guangdong"
                     /lat_lon="30.939972 N 121.33651 E"
                     /collection_date="2016-05-28"
                     /collected_by="Xing Ji"
     gene            complement(join(2685..2990,1..699))
                     /locus_tag="I3K80_RS14205"
                     /old_locus_tag="I3K80_14205"
     CDS             complement(join(2685..2990,1..699))
                     /locus_tag="I3K80_RS14205"
                     /old_locus_tag="I3K80_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_196848181.1"
                     /translation="MQYNTTKCIDENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPTARWLFLTLSTKNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQTEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     gene            complement(882..1703)
                     /locus_tag="I3K80_RS14210"
                     /old_locus_tag="I3K80_14210"
     CDS             complement(882..1703)
                     /locus_tag="I3K80_RS14210"
                     /old_locus_tag="I3K80_14210"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="WP_049948317.1"
                     /translation="MFFKCSICDEKLKRGYKTLACENNHTFDIAKEGYSNLISGHKKSK
                     YDKKLFIARRELNDSSNLYSILISKIIEIIKKYEIKTKNNVIDVGTGEGYILNEISKTF
                     LGENEFLGIDIAKEGVKKAAKSYKNINWLVADLADIPVMNGNVDFLINVLSPSNYDEFK
                     RVLDKEGKLIKIIPNSDYLKEIRNIVFSNHKNKDYDNSEVIDLFEANFNKIEMIDISYK
                     VKLNKEELNKLLDMTPLTWNLSDVEKEGIIKKNLSEITMSFKVLVGGFKTS"
                     /name="putative RNA methyltransferase"
     Typing          complement(2..699)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.857"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2686..2990)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.386"
                     /coverage="100.0"
                     /evalue="5.64e-145"
ORIGIN
        1 ttgaatatct ttatcgcctt tttgatttgg tttgatcgct ttaatatttg ctactggtcg
       61 ataattaact tgtaatgctt tttgccataa atcgacccat tccgtctgcg taatataatt
      121 ttcttttttt ctaaaatacg cattttcaac gcataacaaa acgtgcatat gttgattata
      181 actaccgtca tttttattaa ctgttacttc agttgagcgt aaaaatccta ccaaattctt
      241 tttaactttt gtatacatct ttaatttatt aaaagcctta gacatatgct ttaaactttg
      301 ttctaaatgg tcaccgtcta ttgcattttt agtcgataaa gtaagaaata accaacgagc
      361 agttggcttt tctttaacca cttcttcaat cactttttga gcttgataac tatttttcat
      421 agcacgcctc caattacaaa ctggacacaa ttttgattta caaaaccatg tcttatgtaa
      481 cttcaaataa ccttcatcag taggcttgaa ctctaagacg ttaccacatt gttttacgtt
      541 aaaagccttt ttaattttta agatttctag tatatctgca taacttacat tatctatctt
      601 cttttctctc catgggcgtt gtttcccact tttcgtcata tcttttaatg ttttattatc
      661 ttgattttcg tctatacatt tagtagtatt atattgcata taaaaagacc tccagtattg
      721 atttaagcgt cgtaacttaa tcatattact ggagcttttt tatgtcaatt ttttctttca
      781 cgcacaaaaa tacataaaaa tactgtcata tcaacgataa aacgactttt aatatctttg
      841 agttatttct atatagtatc aagacaagaa gaaactcgtt ttcaactcgt tttaaaacca
      901 ccaaccaaaa ccttaaaact catcgtaatt tcacttaagt ttttcttaat tattccttct
      961 ttttctacgt cacttaaatt ccacgttaaa ggcgtcatat caagtaattt attcaattct
     1021 tctttattta atttaacctt ataactaatg tctatcattt caattttatt aaaattagct
     1081 tcaaataaat ctataacctc agaattatca taatccttat ttttatgatt actaaagact
     1141 atattcctta tttcttttaa ataatcacta ttaggaatta tttttatcaa tttcccctct
     1201 ttatctagaa ctcttttaaa ctcatcataa ttagaagggc taagcacatt aattaaaaag
     1261 tctacattcc cattcataac tggtatatca gctagatcag caaccaacca atttatattt
     1321 ttatatgatt ttgctgcttt ttttacacct tcttttgcaa tgtctatacc taagaattca
     1381 ttttcaccaa gaaatgtttt actaatttca tttaaaatat aaccctcacc agtaccaaca
     1441 tctatcacat tatttttagt tttaatctca tattttttaa taatttctat gatttttgaa
     1501 attaatatcg aatacaaatt gctactatca ttcaattctc ttctagcaat aaacaatttt
     1561 ttatcgtatt tacttttttt atgccctgaa attaaattcg aataaccctc ttttgcaata
     1621 tcaaaagtat ggttattctc acatgctaaa gttttataac ctctctttaa tttttcatca
     1681 catatagaac acttaaaaaa catattcaca cctccaatca aataattata aataacaaag
     1741 ttgctttatt ccaattgctt tattgacgtt gagcctcgga acccttaaca atcccaaaac
     1801 ttgtcgaatg gtcggcttaa tagctcacgc tatgccgaca ttcgtctgca agtttagtta
     1861 agggttcttc tcaacatcaa taaattttct cggcataaat gcgtgttctt ttcttgtctg
     1921 cccagtagac aaagtagacc caactgttat tgctttacag gtctattttt cgtcgttata
     1981 atctagttaa atcccgtaag ggcgcactta tgcgttctaa aaagaacgcg taaaaaacca
     2041 taatcgacct tcgtcaattt ttatcttagt tttaaggaat cggcgttgtg gcgcagatat
     2101 aaaaattaat actgcacgaa ctagaatcga cgttgtagcg tagatataaa aattaatact
     2161 acatagaaat ttaactaaga tttaagctac tagttttttg ctagtagctt ttttattttc
     2221 ttaattttta aatgccttta ttttgaattt taagggggta tttagagcaa actctatatt
     2281 tatatatttt tatacctgta tatttatata tgtttcttaa aacgcaaata tgagcaaaat
     2341 aagggtacat ctttctgaca tttaacttgt attcaaaaga catattactt aagttgctaa
     2401 agtcatttta aatacaatca cttgtacttt ttaaacataa gccagaaaaa cgatataact
     2461 tttttatata tttttctaat ttgattggaa ctccctaaaa tttatataac ttaaaaagtt
     2521 aaattgttta tctcattttt tgtacataac cagtggcgaa tttttcagac actgcccatt
     2581 tacatgcaaa ttaaaatttt catggtaaaa ctggtcaacc atgccggttg aacgctatag
     2641 ttcctgcagg ggcaaaaata cataaaaaaa cgctagcttt taagctaacg tttcaaatct
     2701 tttaagtaat aattttgttt ttcaaaattc caaattgcag taattgaatg tgctttttct
     2761 tcttcgtctg ttgttttatc ttcgtcactt gtattaatta aattgccatc ttcggcatcg
     2821 tctaaattta aaattttatg tttttgttta agcaatccac cataactcaa catacgtttt
     2881 cggtataaac ctttttctaa atcattcacg atttcctgat ttctttcgtc atcatcagtt
     2941 aaaaaatcag atgatttaac cgaatatttc gaggtttctt tgattgctgc
//
