LOCUS       NZ_CP065196             3400 bp    DNA     circular CON 09-FEB-2024
DEFINITION  Staphylococcus aureus strain GDC6P096P plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP065196
VERSION     NZ_CP065196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16871896
            Assembly: GCF_015767495.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ji,X.
  TITLE     Comparative Analysis of Genome, Fitness and Virulence of MRSA ST398
            and ST9 Isolated in Both China and Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Ji,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2020) College of Veterinary Medicine, China
            Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/09/2024 05:25:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,702
            CDSs (with protein)               :: 2,702
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 40 of 86
            Pseudo Genes (incomplete)         :: 39 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 18 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065196.1.
            Bacteria and source DNA available from Veterinary Pharmacology and
            Toxicology Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="GDC6P096P"
                     /isolation_source="pig farms"
                     /host="pig"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Guangdong"
                     /lat_lon="30.939972 N 121.33651 E"
                     /collection_date="2016-05-28"
                     /collected_by="Xing Ji"
     gene            complement(join(2170..3400,1..38))
                     /gene="mobV"
                     /locus_tag="I3K80_RS14190"
                     /old_locus_tag="I3K80_14190"
     CDS             complement(join(2170..3400,1..38))
                     /gene="mobV"
                     /locus_tag="I3K80_RS14190"
                     /old_locus_tag="I3K80_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331753.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053023830.1"
                     /translation="MAYSIIRVAKVKSKTNTKGIQKHVQRQNKNYENMDIDLEKSYLNY
                     DLVNDSPIDYNQKIEEKIEQNYNGKRKIRKDAVKHIDGLITSGNEFFKNQTLEETKQFF
                     EHAKTFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITEDGRLSAKEVLGNKKALTEFQ
                     DRFNEHINSCGYDLSRGITRGVTPRRHEQISRYKNLTDYHKEEYEHESRKLDRIKQESE
                     EVMEQYQNALDVLKKPINVPYELETEKVGGLFNKETQETGNVVIDKNEFDLLQEQVKAS
                     QLITDDYEYIKSGKALKDFEEKNKRLEDRLLDEQIKNGKVIDEYNDLADSYNNLLEQNQ
                     EKEKELNRSYKLFNNVFKLIKGVMKEETYHSLINHIDNHLESSKMRETMIVDDNDEQFF
                     KKKYQRHEPEIIFEDERDDGYTL"
                     /name="mobV"
     gene            complement(233..676)
                     /locus_tag="I3K80_RS14195"
                     /old_locus_tag="I3K80_14195"
     CDS             complement(233..676)
                     /locus_tag="I3K80_RS14195"
                     /old_locus_tag="I3K80_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_196848179.1"
                     /translation="MKNEIPSNYEELKKKSRNKKSWRQRLEAVNELKKYDSKQSRDIIL
                     NLALHDKVFTVKENAFRAAQAMGITKNGEQIKLHKKPKGKLDKNIEKKLTRVVKSLPEG
                     YTFDDFKNEYQKMYPESYDLYEGNKGKSFDKWLKNVESNIPRK"
                     /name="HEAT repeat domain-containing protein"
     gene            complement(722..1648)
                     /locus_tag="I3K80_RS14200"
                     /old_locus_tag="I3K80_14200"
     CDS             complement(722..1648)
                     /locus_tag="I3K80_RS14200"
                     /old_locus_tag="I3K80_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_196848180.1"
                     /translation="MIKKAEEIQAKQSLENENLNFSKTGYSNSRLSGQTLGKSYPKLSF
                     DAVTIVGNLNRINAQKLSEFMSVEPQIRLWDMLQTKFKAKALQERVYIEYDKIKADTWN
                     RRNMRVEFNPNKLTYAEMLWLKQNIVDYIEDDGFTRLDLAFDFEDDLSDYYAVSDKSLK
                     KTIFYGRNGKPETKYFGVRDSDRYIRIYNKKQERKDNADIEVISEHLWRIEIELKRDMV
                     DHWNDCFDDLHILKPAWTTLEKVNEQAMVYTLLHEESMWGKLSKNTKYKFKKLIKEISP
                     IDLTDLMKLTLKNNEKQLQKQIDFWLS"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(731..1580)
                     /name="rep_cluster_1078"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.599"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2174..3399)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.756"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatttcactt tagcaactct gataatagaa tacgccatat taaaattcct ccactgcaca
       61 tgtttttgcg tagctttatt tattaaagta taacacacta gacttattta cttcgtaatt
      121 aagtcgttaa accgtgtgct ctgcgaggct agtttttgtg taaacacaaa aaatggactg
      181 aacaagtcag tccataagtt caaaatataa atcgtaaaat ggatatatca acttattttc
      241 taggtatatt agattccaca tttttaagcc atttatcaaa acttttacct ttatttcctt
      301 cataaaggtc ataactctcc ggatacattt tttgatattc atttttaaaa tcatcaaaag
      361 tataaccttc tggaagtgat ttcactactc tcgtaagttt cttttcaata tttttatcta
      421 atttaccttt tggtttttta tgcaacttga tttgttctcc atttttagtt attcccatag
      481 cttgagctgc acgaaaagca ttttctttta cagtaaacac tttatcatgt aaagcaagat
      541 tcaaaataat gtctcttgat tgtttactat catatttttt taattcattt actgcttcaa
      601 gtctttgacg ccaacttttt ttatttctag atttcttttt caactcttca taattacttg
      661 gaatttcatt tttcataaat ttattcttcc tttctttatc ttaaatatat atattaattt
      721 attatgaaag ccaaaaatca atttgctttt gtaattgttt ttcattattt tttaaagtca
      781 atttcattaa atcagtcaaa tcaattggag atatttcttt aatcaatttc ttaaacttat
      841 atttagtatt tttacttaat ttcccccaca tactttcttc atgtaacaaa gtataaacca
      901 tagcttgctc attaaccttt tctaaagtag tccaagcagg tttcaaaata tgtaaatcat
      961 caaaacaatc attccaatga tcaaccatat ctctttttaa ttcaatttct atacgccata
     1021 aatgttctga aataacttca atatctgcat tatctttacg ttcttgtttt ttattataaa
     1081 tcctaatata tctgtcacta tcacgcacgc caaaatactt tgtttctggt ttaccattac
     1141 gaccataaaa gatagttttc ttgagtgatt tatctgacac agcataataa tcactcaaat
     1201 cgtcttcaaa atcaaaagct aaatctaatc ttgtaaaacc gtcatcttct atatagtcga
     1261 caatattttg ttttaaccaa agcatttctg catatgtcag tttatttgga ttaaattcaa
     1321 cacgcatatt acgtctattc caagtatccg cttttatctt gtcatattca atataaactc
     1381 tttcttgcag agctttagct ttaaacttgg tttgtagcat atcccacagt cgaatttgtg
     1441 gctcaacact cataaactca gataattttt gagcattgat tctattaaga tttccaacaa
     1501 ttgtcacagc atcaaaactt aactttggat aagattttcc caaagtttga ccacttaacc
     1561 ggctattaga gtacccggtt ttagaaaaat ttaagttttc gttttctaag ctttgttttg
     1621 cctgaatttc ttctgctttt ttaatcataa aaaaacaact ccttaattta atttaagaag
     1681 tcgctcaccc gaatatatat cttgaagaat aaacttatat cgtttaatat ctagatatac
     1741 aagatataaa aaaacaactt agtgtttttt ctaggtaagt cgctatcaca aactttggcg
     1801 agggagtgag cgacttcttt ttattttgtt attaatataa cactatcaaa agacttggtc
     1861 taatcagatc aggtcttttt tttatttaag catttgtatt atctggtaaa cagttaaaac
     1921 tactaaaacc ccaagtacat acttacttgc tataaaattc tcaaaagctt ctctaaattt
     1981 catttcttca actcctttta ttgctttatt ccaattgctt tattgacgtt gagcctcgga
     2041 acccttaaca atcccaaaac ttgtcgaatg gtcggcttaa tagctcacgc tatgccgaca
     2101 ttcgtctgca agtttagtta agggttcttc tcaacatcaa taaattttct cggcataaat
     2161 gcgtcgtctt tataaagtgt agccatcatc tcgttcatct tcaaaaataa tttctggttc
     2221 atgcctttga tattttttct taaagaattg ttcatcgtta tcatctacaa tcattgtttc
     2281 tcgcattttt gagctttcta agtgattatc aatatggtta atcaacgaat gatatgtttc
     2341 ttctttcatc acacctttaa tcaacttaaa cacattgtta aatagtttat acgaacggtt
     2401 taactctttt tctttttctt gattttgttc caataaatta ttataactat ccgctaaatc
     2461 attgtattca tctattactt ttccattttt aatctgttca tctaataatc tatcttctaa
     2521 tcttttgttt ttttcctcaa aatcttttaa agcctttccg cttttaatat attcataatc
     2581 atcagtaatg agttgagaag cttttacttg ttcctgcaat aaatcaaatt catttttatc
     2641 aataacgacg ttacctgttt cctgtgtttc tttgttgaac agtccaccaa ctttttcagt
     2701 ttcaagctca tatgggacat ttataggctt ttttagaaca tctaaagcat tttgatactg
     2761 ttccattact tcttcgcttt cttgctttat acggtctaat tttcggctct catgctcata
     2821 ttcttctttg tgataatctg ttaaattttt ataacgactt atctgctcat gtcgtctcgg
     2881 tgtaacgccc cttgttatac ctctactcaa gtcataacca caactattga tatgttcgtt
     2941 aaatctatct tgaaattcag ttaatgcctt tttattacct aaaacctctt tcgcactcaa
     3001 tcgaccatct tctgtaatcg gaactacccc ataatgcata tgaggtgttt tttcgtccat
     3061 atgcactgtt gcatataata aattatcttt tccatactct tgttctaaaa acgttttagc
     3121 atgttcaaaa aactgctttg tttcttcaag tgtttgattt ttaaaaaatt cattaccaga
     3181 tgtaattaaa ccatcaatat gttttactgc gtcttttcta attttgcgtt tcccattata
     3241 attttgttca attttctctt cgattttttg attataatca atcggactat cattgactaa
     3301 atcgtaattt aagtatgatt tttctaaatc aatatccata ttttcataat ttttattttg
     3361 tctttgtaca tgtttttgaa tgccttttgt attcgttttt
//
