LOCUS       NZ_CP065170             3223 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPN114 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP065170
VERSION     NZ_CP065170.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16685806
            Assembly: GCF_015694085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L., Jenney,A. and Holt,K.E.
  TITLE     Diversity and transmission of ESBL plasmids in a Melbourne hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: KPN114
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 13:21:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,360
            Genes (coding)                    :: 5,212
            CDSs (with protein)               :: 5,212
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 61 of 148
            Pseudo Genes (incomplete)         :: 83 of 148
            Pseudo Genes (internal stop)      :: 30 of 148
            Pseudo Genes (multiple problems)  :: 22 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065170.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN114"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2017-05-18"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            complement(133..456)
                     /locus_tag="I3U24_RS27245"
                     /old_locus_tag="I3U24_27245"
     CDS             complement(133..456)
                     /locus_tag="I3U24_RS27245"
                     /old_locus_tag="I3U24_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1474..1806)
                     /locus_tag="I3U24_RS27250"
                     /old_locus_tag="I3U24_27250"
     CDS             complement(1474..1806)
                     /locus_tag="I3U24_RS27250"
                     /old_locus_tag="I3U24_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1974..2189)
                     /locus_tag="I3U24_RS27255"
                     /old_locus_tag="I3U24_27255"
     CDS             complement(1974..2189)
                     /locus_tag="I3U24_RS27255"
                     /old_locus_tag="I3U24_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2193..2678)
                     /locus_tag="I3U24_RS27260"
                     /old_locus_tag="I3U24_27260"
     CDS             complement(2193..2678)
                     /locus_tag="I3U24_RS27260"
                     /old_locus_tag="I3U24_27260"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     ARG             complement(1475..1806)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(553..744)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          724..1207
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(137..456)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="8.510000000000001e-64"
     Typing          complement(1877..3222)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.796"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgaggcgt cgataagctc ccggacttca tccggcttcc cctgcagaac gctggcgccg
       61 tcgcgctggc gatctttacc cagcagataa tcgaccggcc cgccgccgcc gccgcgcccc
      121 cgaggatgaa acttaacgat catcatcagc acccttttcc agtacggcat gccgcaaccg
      181 ctcgagtccg gcatcgatgg ccatcagcgc ggcgacaatc tgcacgcggt cgtgtccgct
      241 tccaccaccg gcgttaacct ggcgggcaat ctggttaagg ttgttcccca tgccggcaag
      301 ctgacgaagc agcgccggcg agatcgacgg aagtttgccg gcgcgtgcgg gcttctcgtc
      361 caggcacgtc tgccgcatcc aggccgcgag ttgcttaccc tcgcagcgct ccagcagacg
      421 ccggtgctcg tcctcagtca cccacatggt gagcattttg ctgcgtttgt ctgccaatcc
      481 ggcctcctga taacgccggg acggacggga aatcttttcc cggtcggcac ggtgttggac
      541 aagccgcagg cgcggcagtg gttttagcgg gtttcggggc gcagccctga accagtcacg
      601 tagcgctagc ggagtgtata ctggctaaac atgttatgac ggagtggcaa tttatgaaag
      661 tgcttcatgt gacaggggaa aaatgcggct gccgggcgta agtgcaaaat gcaggtggtg
      721 atgaaatgac gcctcctcgc tcactcggtc gctacgctcc tgccgtgaga ctgcggcggg
      781 cgttaccagc tcacagatta cgggataagc aaatgccgct cgaagcagga aaggccaaga
      841 aaaggatgcg gagttgccgt ttttccatag gctccgcccc cctgacgagt atcacgaaaa
      901 tgacgctcaa atcagcggtg gcgaaacccg atgcggctta aagataccag gcgtttcccc
      961 ctggtggctc cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt gtcattccgc
     1021 tgttatggcc gcggtttctc attccacgcc tgaaactcgg ttccgggtag gcagttcgct
     1081 ccaggatgga ctgtatgcac gaaccccccg ttcagtccga ctaccacgcc cgttccggta
     1141 actatcaact tgagtccaac ccggaaagac acgacaaatc gccagtggcg gtagccattg
     1201 gtaactgggt tcacggagca gagattgtag agaatgccta cagagttctt gaagtgaaag
     1261 cctgagtgcg gctacactga aaggacagtt taggtatctg cgctccgtga aagccagtta
     1321 ccatcggaac aaagagttgg tagctctgaa ttccggcaaa cgaaccaccg ttggtagcgg
     1381 tggttttttt gtttacaggg aaaaagatta cgcgcagaaa gaaaggatct caaggaaatc
     1441 cttttgcttt ggccaatatg cttcggtaaa aagttaatgc gcagaagatt ttgaaaaaat
     1501 attaataacc aaaactccaa cgcaaataag gaacattcct gcaattgcgg gccaatcaag
     1561 tttttgaccg ctaaccaacc aaccagctag gcttattagt acaattcccg caccagacca
     1621 aattgcgtaa gcaataccgg taggtatatt ttccaatgtt tgggaaagca gataaaaggc
     1681 agcgcaataa cttattgcag ttcctacaga cggccacagg cgtgtaaatc cttctgaata
     1741 tttcataaag gtggtgccta tgacttcagc cacaatagcc cctcccagga aaatatatgt
     1801 attcataaaa atacctcatg ttgaaaaatt taaatatctt tacataacaa ctaattaata
     1861 gaacccggct tatcatagcg aaggaccatg atgctgccac tgcgatcgtt ccagctccag
     1921 cgctttctgg tgctgcaccg cccgctcctg ctgccggtga gtttctatcg cgttcagcct
     1981 ttcgacaacc ggcgcagctg ctcgctcaaa ctctgcacct gcctgctcaa gaccgtgacg
     2041 cgctcgttca gcgccgcgtt ctcccgttga ataagaccag acatcgtccg ccattccgcg
     2101 aaggcgttct cccactcgtc cagccttttc gagtagtcct gctgtagctg ctctaatgcg
     2161 ctcagcaact gtttttccag ctccgtcatg tgctatttcc ccgccagtat catccagctc
     2221 gagtcctctc cgaactgtcc cgcttccggg ttcaccctca cgcagcggcg tctctgctcg
     2281 ccgcaggtcg agagctgtac gccgctgttc ctctctgaca gcatggcctg cgtgctcttc
     2341 tgctcccgga tggtcgtata gttctcgagt atctgctgcc cctgccacca cagaatgccc
     2401 gcgctcgacg ctatcagcag cgcggagacc agaacgatgg tcagccacgt ctggctgacc
     2461 atgcgcagca tccctttcgt gcgctggctc atggctgagg acagcttccg gtcgtggtcg
     2521 aggatggcgg cgctgattct cttctcgctt tcgctcagtt ccgctctgac agacttctcg
     2581 tgctcgctga atgcggcttt cagcatctcg ccggtagtct gctgctgcgc tttcgatttc
     2641 tgctctaagt ccttcgccag cgttaaaaga ctgttcatag atggctccct tcagtcggat
     2701 gtttcgcccc ccgtccgggt cggcaatgct gatgctgctt tttgtggtgc gcaccacctc
     2761 aaaacctgcg ctttccagcg cctcagtgac gtcctgacgc gttttcagct ccccggatga
     2821 ggcaagggca agcaggccgc tcgtaatcgc ctgggcggct tcctgcttcg cttccggcag
     2881 cgcggaaggc gagaccagca cccgccggtt ctccggcgcg ttcgggtcat gcagccccag
     2941 cctgccgttc actatggtct gccaggcatc gatgcgcgga cggtcggcgc ggtcgtaata
     3001 cggctgtagc cgcttgccgg tcagaagctc cgtgttcggg atcaggaagt tcagctccag
     3061 ccgccccttg tcccggtgct caacccacag cacgctgtac tggtctttat cgagtccggg
     3121 catcagaacc cgctcgaaac tcgccatcag cttttcgcgc tggccgggcg gtaaatcctg
     3181 ttcggcaaaa gacagaacgc cggaagtgta ctttttggcg tag
//
