LOCUS       NZ_CP065169             3514 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPN114 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP065169
VERSION     NZ_CP065169.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16685806
            Assembly: GCF_015694085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L., Jenney,A. and Holt,K.E.
  TITLE     Diversity and transmission of ESBL plasmids in a Melbourne hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Hawkey,J., Wyres,K.L., Judd,L., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: KPN114
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 13:21:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,360
            Genes (coding)                    :: 5,212
            CDSs (with protein)               :: 5,212
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 61 of 148
            Pseudo Genes (incomplete)         :: 83 of 148
            Pseudo Genes (internal stop)      :: 30 of 148
            Pseudo Genes (multiple problems)  :: 22 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065169.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN114"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2017-05-18"
                     /collected_by="Microbiology Unit & Department of Infectious
                     Diseases, Alfred Hospital"
     gene            complement(218..559)
                     /locus_tag="I3U24_RS27220"
                     /old_locus_tag="I3U24_27220"
     CDS             complement(218..559)
                     /locus_tag="I3U24_RS27220"
                     /old_locus_tag="I3U24_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1730..1921
                     /locus_tag="I3U24_RS27225"
                     /old_locus_tag="I3U24_27225"
     CDS             1730..1921
                     /locus_tag="I3U24_RS27225"
                     /old_locus_tag="I3U24_27225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071008427.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     gene            complement(2335..2550)
                     /locus_tag="I3U24_RS27230"
                     /old_locus_tag="I3U24_27230"
     CDS             complement(2335..2550)
                     /locus_tag="I3U24_RS27230"
                     /old_locus_tag="I3U24_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2557..3054)
                     /locus_tag="I3U24_RS27235"
                     /old_locus_tag="I3U24_27235"
     CDS             complement(2557..3054)
                     /locus_tag="I3U24_RS27235"
                     /old_locus_tag="I3U24_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     Typing          complement(645..817)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          742..1288
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..228)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="90.0"
                     /evalue="1.43e-40"
     Typing          complement(225..538)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          complement(2220..3514)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctggccatc agtttttcac gctgacccgg cggcaggtcc gcctccgcga atgacagcac
       61 cccggaggta tattttttca catacggcga cgcatcaatc agctcccgga cttcttccgg
      121 cttcccctgc aggacgctgg caccttcacg ctgccggtct ttacccagaa gataatccac
      181 aggaccggcg cccccgccgc gaccccgggg atggaattta acaatcatcg tccggccctt
      241 tttccagtac ggcatgccgc aaccgttcca gaccggcatc gatcgccatc agcgctgcca
      301 cgatctgcac ccggtcatga ccggcaccac cggcgttcac tttccgggcg atctggttca
      361 ggttattgcc catgccggca agctgacgca gcagcgcggg cgaaatcgag ggaagcctgc
      421 cggcgcgtgt cggtttctca tcaaggcagg tctggcgcat ccacgccgcc agttgttttc
      481 cgtcgcaccg ctcaagcaac cgccggtgtt cgtcttccgt cacccacatc gtgagcatct
      541 tgttgcgttt atctgccaga atcgcctcct gataacgccg tgacggacgg gaaagccttt
      601 cccggtcggc gcggggttgg acaagccgca ggcgcgtcag tggggtttcg gggcgcagcc
      661 ctgaaccagt cacgtagcgc tagcggagtg tatactggct tagtatgttg gcatgagtgc
      721 gataatcagg aaagcgcctt atgcggcaga agaaaaatgc tgcagcagag cgtaagcaga
      781 atatgcgata cagaagatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      841 ggctgcggcg agcggtgccg gcttacgggc ggggcggaaa tttccaggaa gataccagga
      901 agaaacttaa cagggaagcg atgaggccgc ggcgaagccg tttttccata ggctccgccc
      961 ccctgacaag catcacaaaa tctgacgctc aaatcagtgg cggcgaaacc cgacaggact
     1021 ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgcctt
     1081 tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac
     1141 tcagttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1201 ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacacgcca
     1261 aagcgccact ggcagcagcc actggtaaca ggattagcag agcgagttaa tgtaggtgat
     1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctggaaggac agtatttggt
     1381 atctgcgctc tgctgaagcc agttacctgg ttaagcagtt ccccaactga cttaaccttc
     1441 gaaaaaccgc cctgcaaggc ggttttttcg tttacagagc aagagattac gacgaggctg
     1501 aatgaatctc aagatttcat ttagcctgcc acccgatcat tcaaagtttt ggcgtagttc
     1561 tgacccgctc cccgtgattt gttacatacc caccacaggg cattagtttt tttatttttt
     1621 tgatgatatg tacaaaatca gtggttagga ataatcttag cagccttaac aaacacacac
     1681 aaaaacaacg ataatgattc atctgacgac aacaaacaat gacaatgtca tgttaaaaaa
     1741 aaatgaaatt atcttaataa aatcatgtgg taactcaaaa acaaaaattt ttttacgagg
     1801 tgtggatgag ggactcatga ttgatgcaag ttttgaagat ataaaatttg ctatcaacaa
     1861 taacctccac gatcttctcg gaatccaagc cgaaaccaca aaactaaaac taacaaagtg
     1921 atacagcccc tgaagcatga aggatcgaaa taaagccacc gatttaccat aacgtcttct
     1981 tacccgcacc gcgccggagg cactcagggc gtttctgatg gctgacgggc aaaaggtggt
     2041 ggcaagtaca ccgaaaacca ccagaaacgc acctgattta ttttacgcat atgcatcact
     2101 tattcacatc actgcatagc cggtgatttc ggttaaaaaa gactctctgt gtgctgaaat
     2161 cgcagaaaat ggccatgatc cccgtcacgc tcaggccacc tgagacgggc gtttatcagt
     2221 ctccatactc cctgtcacca tgagataatg tgcgttgccg ttccggtacc agatctgctg
     2281 tcttctctgg ttccttttcc gttgtttgcc cgtatcgctg ttgctcaatc tcattcagcc
     2341 tgtcgataac cggcgcagct gctcgctcaa actctgcacc tgcctgctca agcccgacac
     2401 gcgctcgctc agcgccgcgt tctcccgttg cataagacca gacatcttcc gccattcctc
     2461 gaaggcgctc tcccactcgt ccagccttct cgagtagtcc tgctgtagct gctctaacgc
     2521 gctcagcaat tgtttttcca gttccgtcat tctgctctac tcccgtacca gcttcaccgc
     2581 gttccgcgtc ttgttgtcca ccgtccggcc cgtttcggct ttcatcccct ccggcagcac
     2641 cagaaaccgt ccgttgcggt tctccagata ggtcacgccc cacgtctgca tgtgcagttt
     2701 cgacagcgta tctctctgct gcctgatttc cgccaggttc gccgctatcc ggttcccctg
     2761 gtaccacagc accccgctca ggctggcgaa cagcagccct gacaccatgg tgatggtcag
     2821 ccacgtccgc ccgaccattc gcagcacgct cagccggttc gactgcatcg ccgcgttcat
     2881 cccctgttcg tgggcgctga tggcgtcgtt gattctcttc gcgcttgcgt tcagttccga
     2941 tttgacaacg ttctcgtgct tgctgaatgc ggctttcagc ctctcttcgg taagctgctg
     3001 ctgtgctttc gatttctcct cgaagtccct cgaggccgtt aaaagactgc tcataaatgg
     3061 ctcccctcag tcggatattt cgccccccgt ccgggtcggc aatgctgata ctgttttttg
     3121 tggttctgac cacctcaaaa ccggccgctg tgagcgcccc tgtgacgtca tcacgcgttt
     3181 tcagctctcc tgatgaggct aacatcagca aaccccgtgt aatcgtctct gcggcttcct
     3241 gcttcgtttc tggcagtcct gaaggcgtga ccagcgcccg ccggttctcc gggtcgttcg
     3301 gatcatgtag ccccagcctg ccgttgacca ctgtctgcca cgcatcgatg cgcggccggt
     3361 cggcacgatc gtaatacggc tggaggcgtt tccccgtcag taattcggta ttggggatca
     3421 gaaaattcag ctcaagacga cccttgtctg cgtgttccac ccacagaatg ctgtactggt
     3481 ctttatcgag tccgggcatc agaacccgct caaa
//
