LOCUS       NZ_CP065127            10047 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica strain 172 plasmid p172-10k,
            complete sequence.
ACCESSION   NZ_CP065127
VERSION     NZ_CP065127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16807073
            Assembly: GCF_031036295.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica
  ORGANISM  Salmonella enterica subsp. enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Huang,T.-W.
  TITLE     Whole-genome sequencing of ceftriaxone-resistant Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Huang,T.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2020) Department of Microbiology and Immunology,
            School of Medicine, College of Medicine, Taipei Medical University,
            No. 250, Wuxin Street, Taipei 11031, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 230x
            Sequencing Technology             :: Illumina, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 16:22:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,726
            CDSs (total)                      :: 4,605
            Genes (coding)                    :: 4,500
            CDSs (with protein)               :: 4,500
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 37 of 105
            Pseudo Genes (incomplete)         :: 78 of 105
            Pseudo Genes (internal stop)      :: 24 of 105
            Pseudo Genes (multiple problems)  :: 28 of 105
            Pseudo Genes (short protein)      :: 1 of 105
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065127.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Salmonella enterica subsp. enterica"
                     /mol_type="genomic DNA"
                     /strain="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:59201"
                     /plasmid="p172-10k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2008-07"
     gene            172..417
                     /locus_tag="I0P30_RS23565"
     CDS             172..417
                     /locus_tag="I0P30_RS23565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256382264.1"
                     /translation="MQPQPAVHDGLPGIDARTVSAVVIRLEPFAGQQLRVRDQEVQLQP
                     PLVLVLHPQHAVLVFIESGHQNTLEARHQFFTLTGR"
                     /name="hypothetical protein"
     gene            complement(599..922)
                     /locus_tag="I0P30_RS23570"
                     /old_locus_tag="I0P30_23445"
     CDS             complement(599..922)
                     /locus_tag="I0P30_RS23570"
                     /old_locus_tag="I0P30_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1572..1730
                     /locus_tag="I0P30_RS23575"
                     /old_locus_tag="I0P30_23450"
     CDS             1572..1730
                     /locus_tag="I0P30_RS23575"
                     /old_locus_tag="I0P30_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            complement(1973..2278)
                     /locus_tag="I0P30_RS23580"
                     /old_locus_tag="I0P30_23455"
     CDS             complement(1973..2278)
                     /locus_tag="I0P30_RS23580"
                     /old_locus_tag="I0P30_23455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_223229636.1"
                     /translation="MPKPDNSCCRSEDIVCRFGGEEFVVFLPNTSVVTAECVAERIRSV
                     IESTVFPNNLRVTVSAGVATQEDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(<2264..3294)
                     /locus_tag="I0P30_RS23585"
                     /old_locus_tag="I0P30_23460"
                     /pseudo=""
     CDS             complement(<2264..3294)
                     /locus_tag="I0P30_RS23585"
                     /old_locus_tag="I0P30_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            3689..4345
                     /locus_tag="I0P30_RS23590"
                     /old_locus_tag="I0P30_23465"
     CDS             3689..4345
                     /locus_tag="I0P30_RS23590"
                     /old_locus_tag="I0P30_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(4664..5278)
                     /locus_tag="I0P30_RS23595"
                     /old_locus_tag="I0P30_23470"
     CDS             complement(4664..5278)
                     /locus_tag="I0P30_RS23595"
                     /old_locus_tag="I0P30_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            5440..>5622
                     /locus_tag="I0P30_RS23600"
                     /old_locus_tag="I0P30_23475"
                     /pseudo=""
     CDS             5440..>5622
                     /locus_tag="I0P30_RS23600"
                     /old_locus_tag="I0P30_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            5758..6303
                     /locus_tag="I0P30_RS23605"
                     /old_locus_tag="I0P30_23480"
     CDS             5758..6303
                     /locus_tag="I0P30_RS23605"
                     /old_locus_tag="I0P30_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            6440..7012
                     /locus_tag="I0P30_RS23610"
                     /old_locus_tag="I0P30_23485"
     CDS             6440..7012
                     /locus_tag="I0P30_RS23610"
                     /old_locus_tag="I0P30_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            7049..>7654
                     /locus_tag="I0P30_RS23615"
                     /old_locus_tag="I0P30_23490"
                     /pseudo=""
     CDS             7049..>7654
                     /locus_tag="I0P30_RS23615"
                     /old_locus_tag="I0P30_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(7582..8958)
                     /locus_tag="I0P30_RS23620"
                     /old_locus_tag="I0P30_23495"
     CDS             complement(7582..8958)
                     /locus_tag="I0P30_RS23620"
                     /old_locus_tag="I0P30_23495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900944.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_080196049.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFISAPYKSA
                     SGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISIVSDDGNV
                     IFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAWSVLVYSH
                     ADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKDIGGSLVD
                     IKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSNKIQNHKQ
                     GSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRGQPERKPE
                     "
                     /name="diguanylate cyclase"
     gene            complement(9157..9405)
                     /locus_tag="I0P30_RS23625"
     CDS             complement(9157..9405)
                     /locus_tag="I0P30_RS23625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658416.1"
                     /translation="MGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVA
                     RLNGIEAHREQERAAQHRKALELERSQRQRTYNGPSL"
                     /name="hypothetical protein"
     gene            complement(9483..9968)
                     /locus_tag="I0P30_RS23630"
                     /old_locus_tag="I0P30_23505"
     CDS             complement(9483..9968)
                     /locus_tag="I0P30_RS23630"
                     /old_locus_tag="I0P30_23505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     ARG             3690..4342
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1193..1739
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.652"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..609)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.044"
                     /coverage="100.0"
                     /evalue="3.29e-125"
ORIGIN
        1 ctcaaaaccc gcgttttcca gcgcctcagt aacgtcctga cgggttttca gctccccgga
       61 cgaggctaag gccagcaaac cccgggtgat cgcctgagct gcctcctgct tcgtttccgg
      121 cagcgcggac ggcgtgacca gcgcccgccg gttttccggc gcattcgggt cgtgcagccc
      181 cagcctgccg ttcacgatgg tctgccaggc atcgatgcgc ggacggtcag cgcggtcgta
      241 atacggctgg agccttttgc cggtcagcag ctccgtgttc gggatcagga agttcagctc
      301 cagccgcccc ttgtcctggt gctccaccca cagcacgctg tactggtctt tatcgagtcc
      361 gggcatcaga acacgctcga agctcgccat cagtttttca cgctgaccgg gcggtaagtc
      421 ctgttcggca aacgacagca ccccggaggt gtactttttg gcgtagggcg aggcgtcgat
      481 tagctcccgg acttcctccg gtttaccctg cagaacgctg gcgccgtcgc gcttgcggtc
      541 attacccagc agataatcca ccggaccggc accgccgccg cgccctcgtg ggtgaaattt
      601 aacgatcatc gtcagccccc ttttccagca cagcgtgccg caggcgctca agtccggcat
      661 caatggccat cagcgcagcg acaatctgta cgcgatcatg tccggtacca ccaccggcgt
      721 taacctgccg ggcaatctga ttgaggttgt ttcccatacc ggcaagttga cgaagcaacg
      781 ccggcgagat cgacggaagt ttggcggcac gagcaggttt ctcgtcgaga cacgtctgcc
      841 gcatccaggc cgcgagctgc ttaccgtcgc agcgatccag cagacgccgg tgctcgtctt
      901 cagttaccca tatcgtgagc attttgctgc gtttgtctgc caatccggcc tcctgataac
      961 gccgggacgg acggggaatt atttcccggt cggcgcggtg ttggacaagc cgcaggcgcg
     1021 tcagtgcctt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     1081 gtgtatactg gcttaaaatg taacatcgca gaaacatcaa gcatagctgc cacataaagc
     1141 aggtgaaaaa tgagattcgt atgcgtcagc agaagatgca ctgccggaga aatgccgctt
     1201 cctcgctcac tgactcgctc cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg
     1261 ggcggggcgg aaatttcctg gaagatgcta ggaagaaact taacagggaa gcgataaggc
     1321 cacggcagag ccgtttttcc ataggctccg cccccctgac aagcatcaca gaatctgacg
     1381 ctcaaatcag tggtggcgaa acccgacagg actataaaga tcccaggcgt ttccccctgg
     1441 tagctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1501 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     1561 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctcc tccggtaact
     1621 ttcaacttga ttccaacccg gaaaggcacg acaaagcgcc actggcagca gccattggta
     1681 actgggaatt agtggattta gatatcgaga gtcttgaagt ggtggcctga ctacggctac
     1741 actgaaagga cagtatttgg tatctgcgct ccactaaagc cagttacctt cggaagaaag
     1801 tgttggtagc tcttaattcc ggcaaacgaa ccaccgttgg tagcggtggt ttttttgttt
     1861 acagggcatg agcttacgac gacatcgaag ggataacaag aggatccgct tcttgaaccc
     1921 ttttccccac aaaaaaacgc cacactagcg cgtaaagcgt catcaaaaaa actcaaatag
     1981 cgctatatac aatcacttta ttacgcccct ctcccttggc ttgataaaga gcatcatcag
     2041 catttttcag caaaaaatcg atcccaccaa gaggatcctc ttgtgttgcc acaccagcag
     2101 agacggtgac ccgcaagtta ttagggaaaa cagtactttc aataacactc ctaatacgct
     2161 ccgcaacaca ctccgctgtt accacagatg tattcggcag aaaaacaaca aactcttctc
     2221 caccaaaccg acaaacaata tcttcactac ggcagcaaga attatccggt ttcggcatga
     2281 tgctgatgta gtcccgtttt atcgtcatca cgaagctttc tgctatgcca ttgctttccg
     2341 ggcttcgcac tgctgtcgta caaggctcca gtcccagcaa ccgggcgaac gccggcactg
     2401 ttggcaactg ccttccgaag cgtttttcca ccgcacccag catgacatcc tgcaccgttt
     2461 ctttgtcata gcctccggtg ctcgctgccc aatcaatgat ctccctgtcg cagcagtcct
     2521 gcgcgaaggt cacccgcagc ttttcgccat tatcacagcg gaactcaaag ccatctgagc
     2581 accagcgccg gttactttcc gctactgcta cgcgcccctt atgggcccgc ttacggcaag
     2641 gatcagcggg tttacgttca agaagcagat tatgcgttct cataatgcga tacacccgct
     2701 ttgcattaac cacaggcagg ccgtcccgct ccgactcccg tcgcagcagc gcccacacac
     2761 ggcgataacc atacgtcggc aggtcagcca ccgccatgtt tatccgggac aatacggtgg
     2821 tatcgtcaga acggggctgc cgtctgcgat cctgtcagtc agatggccgg tgaacccgaa
     2881 tgctcaactg cgcacgcgac acgccaagac ttctgcaaac gtcgcttatt cgtcgttccc
     2941 aggcaacgag ggcgcatgcg caatccattt ttttgcccgg ccgaactcca cggcctcttt
     3001 gagtatctcg gcttccatcg tctttttgcc cagaaggcgc tggagttcgc gaatttgctt
     3061 attggcggca gcgagttcgg aggccggcac cacttcctcg cccgatgcaa cagccgtcag
     3121 tgaaccgtct taatattgct tgcgccattt gaatatctgg tttgcattga tgccatgcag
     3181 gcgcgcgaca tgagacacgg tcataccggg ctccatagtc tgctgaataa tggcaatttt
     3241 ttcctgcggt gtacgacgcc gacgccgctc aggtcctgat aacacttcaa ccatcttatc
     3301 gttctgactg gtattaaaca tagttccaag actacctctt attttaagag agtcgaagtg
     3361 tctggtgatc tatggggcca gtctagccct cttccaagtg tagggagata tagtcaaagt
     3421 ttgcttctcc gccgatgagt ccgccctgcg tcggatagtc cgatttttta ccggcgaagc
     3481 acggctctcc taaccctatc taaagcgaat aacttttttc aacagggccg cgtgccaatg
     3541 caagagcgat aggcgctcta cgcgccaatt tgaccactta aaacaggtaa attgaggggt
     3601 tgtaatgtgt tgatgtaaca ggcttttatt ttaatgtctt ggcatatgta taatggtagt
     3661 ctagccctcc tttcaacaag gagtactcat ggaaacctac aatcatacat atcggcacca
     3721 caacttttca cataaagact taagtgatct caccttcacc gcttgcacat tcattcgcag
     3781 cgactttcga cgtgctaact tgcgtgatac gacattcgtc aactgcaagt tcattgaaca
     3841 gggtgatatc gaaggctgcc actttgatgt cgcagatctt cgtgatgcaa gtttccaaca
     3901 atgccaactt gcgatggcaa acttcagtaa tgccaattgc tacggtatag agttccgtgc
     3961 gtgtgattta aaaggtgcca acttttcccg aacaaacttt gcccatcaag tgagtaatcg
     4021 tatgtacttt tgctcagcat ttatttctgg atgtaatctt tcctatgcca atatggagag
     4081 ggtttgttta gaaaaatgtg agttgtttga aaatcgctgg ataggaacga acctagcggg
     4141 tgcatcactg aaagagtcag acttaagtcg aggtgttttt tccgaagatg tctgggggca
     4201 atttagccta cagggtgcca atttatgcca cgccgaactc gacggtttag atccccgcaa
     4261 agtcgataca tcaggtatca aaattgcagc ctggcagcaa gaactgattc tcgaagcact
     4321 gggtattgtt gtttatcctg actaattgct ttgatgtgtg attttaaacg ctcaaattta
     4381 taaaacgaat aatattgtca accttgcaat aatagacata gggacaacca tgtataaatt
     4441 tttaccctca ggcgtcttca caatagagcc atatagcagt gggtcactta gtgggcttaa
     4501 tttttccatt aaagataata ttaatattgc tcagtataaa acatcatatg gtagcccatc
     4561 ttggcaagta aacataaggc tgccatttat aaggcactgt tgcaaaaatg tggaggtgat
     4621 aggggtacta cgccggaacc ccagattttt ccgtccgttc aaattatcca cggttgaaca
     4681 gcttgatccc gtccgcccgc agttgaccaa caggggaaat atgcctgtac aaagtttgcc
     4741 gggttatccc aagttcctgg catagcgtac tgacctttgt ttctgattgt cccattgatg
     4801 ccattgccag tcgcagtttg actggcgtca ttttataagg tcggccgccg ttccgcccac
     4861 gggctcttgc tgatgccaga cctgcagtcg ttctttcagc aatcagttcg cgctcaaact
     4921 ccgccagtgc ggcaaagata ccgaagacaa gcttgccagc ggccgttgtt gtgtcgatag
     4981 tcgccccgtg accggtcagc actttcagac cggtccccct cgcggtcagg tcgtgcactg
     5041 tattaatgag atgacgaaga tcacgaccga ggcggtccag cttccatacg accagcgtgt
     5101 ctccgggtcg tagtgctttc agacaggctg taagtccagg acgctcttct cgtttgcccg
     5161 atgcccggtc ctcataaaaa tgtgcggcat caacacctgc ggcgatcagt gcatcacgtt
     5221 gtaaatcatt cgtctgcgaa ccatcagctt ttgacatccg aatatatccg atgaacatat
     5281 ttcctctgtc acatatacgt tcgattatgt gacattctga tttgggtacc ataatctgtc
     5341 aacggtagtc acattacccg taatttaaat tatgacacgt aaaggttcta tgttaatgat
     5401 aatgtgacaa gcgcgtttaa ataggcataa ggatcagaga tgcctactaa aaataagctc
     5461 ctcagtattc tttcggacgc cgagcaggaa gcgctctatg gtctacctga tttcgatgat
     5521 gcacagcggc tggagtttct ggcgctgaat ggatatgaac tggcgctggc ctgcagtcgc
     5581 cgggggcttc acgctcaaat ttattgcatc atccagatcg gagagcatga aaaacctcgg
     5641 ggcagccatc gaatgatgcc atctacaggg gttaaaataa ccaccatcat ttcataacaa
     5701 tacctgctga ctatgaagat ctacgtcatc aaaatagcag ttcatggagt cagtccgatg
     5761 gtctggcgtc ggctgagaat tgctgccgac acgtcactgg ccgcgcttca cttcattttc
     5821 cagatagtgc agggctgggg ggacgaccat ctccatcaat ttcacattta tggcaaagat
     5881 tacggtatct cctacgaagg aggcatcggt tttgtcgata atccgttccg ggtcgtgatt
     5941 gatgattttg cttttgatgc tggcgatcgc tttacctatg aatacaattt ctttgagcac
     6001 tggcttcatg acattcgtgt tgaggccatt tatgaaaact ctacgctgaa agcgccgttt
     6061 tgtataagcg gccatggtat gcccggagcc acagctgcgg atgagttcga taaaaccctg
     6121 gcgtttcttg aagctatcgt taatgcggat gatgaaacaa cggtcggcga gatccgccct
     6181 tttgctgacg acctggatgc tgtcaggttc aaccgccaca aaatcaaccg gcaactgagc
     6241 aggctcgacc ttgcatctcc ggtactggag cctgaagtta tctggctggg ccgtcgtcgc
     6301 tgactgatgt ctataaaggt atacgttttc agcatgcctc tcagatcccc gaaaaaatgt
     6361 ctataaacct ccctaaatca gtgtttatgg attattcttt aaatctctat gatgtttaaa
     6421 gaccttccgg aggttttgcg tgcaatatgc ctacatcagg gtaagctcgg ccgatcagaa
     6481 tacggcccgt caggaagagg cattgtcaaa ggcgggtttt cagcctgata aaatttgtgt
     6541 tgagcatgcc agcgcgaagg acacaaaccg cccggggtta caggaactcc tggggcaatt
     6601 acgccctggt gatacgcttc tggttcattc catcgatcgc ctttgccgga atatgtcgga
     6661 tatgtgcgct gtcaccaccc ggcttcgtga ccagggcgtt acccttattt ttctgaaaga
     6721 gcagctgacg ttcagcgccg gcacaaacaa tccgatgcag gaactgcagc tgcacatgat
     6781 gtcggcattc agccagtttg aacgagcctt actgaaggaa aggcaggccg acggcatcgc
     6841 cgcgaaaaaa gcacggggtg aaaaaacagg gcgccccggc gccgatatca aaaagatcca
     6901 ggaaattgac gcactcagaa gcagaggggt caggctcagg atcgcctgcg accatgcggg
     6961 ccttggcgtt tccacatatt ataagcttcg tcaccagatg aacaaccagt agccgggaga
     7021 tagtcatcca ccacagcagg aggcaatgat gcaactgacg ctccagatag tcatcaccga
     7081 tgaatcaggc tccagtcgaa cggaggaact gatgacaatc cagaaatcag gggagacccg
     7141 gaacgacatc ggattatcgg tgtcagaatc caagctgttg ctgaatacgg tccagcagtc
     7201 ggtggtccag ctgcaggcag acgaatatac tcaacaccat atccggtgcc ctcactgcct
     7261 tgctgcgcgc agaatcaaag gcaaacagaa aatacggtac cggacgctgt ttggcgttat
     7321 tccggtatcc gggcttcggg tgtaccggtg ccgttgtgaa gagagtgata caaagacagt
     7381 cagtttgctc agcgactggg ccggcgatta ttctcacccg gcactgaaat atattgaaac
     7441 ccgctgggcc tcgatgatct cctatgaaat gacgactcgt ctgctgaaag atattctgcc
     7501 ggtgggccac agcctgaatg cctcaacggt gaggaatcat ttatgccagg tggcgcagcg
     7561 tcttgacgct gaggctgaag ctcattcagg ttttctttcc ggctgccccc gcgactgggg
     7621 gaacctgccc aggccaggaa aaccgcaatt aataacctga gccaaagtaa caagaacctc
     7681 atctccgacc ccatgcccga agttatcatt tattttttta aaaaaatcaa catcaatagc
     7741 aataactgaa gcccctgaac cctgcttgtg attttgtatt ttatttgaaa accccagtct
     7801 gttgttcacg ccggttaggg gatctttaag cgccacctca tttagagaat caactctttc
     7861 tatcattatc ttaatatgcg taaaaagtgc cttttttagt cggtcggctt cgacatacca
     7921 agcattgata cctttaatat caactagcga accccctata tctttagcat tggtagatat
     7981 agcaagcttt tctaaaggaa ttgaaatacg ggaagcaatg aaatatgaaa aaagtgaaaa
     8041 tgtaataact attacaatta gcagaacaag tacactctta gcataagtaa taagaatagt
     8101 agtcacgctg tcagcgtgac tataaaccaa cacactccaa gcagtatttt tcatatgagc
     8161 ataaccaaga agatatttat tccccgaaga aaaaaagata ccctcacctc tctcagacga
     8221 agttaacttc gatttcaaat tatcatccat aaccattggt cgaccaacag cactgttatc
     8281 gttattaaag atcacatttc catcatcact aacaatgcta atttcagtgt cgcttttgaa
     8341 aaaatgcctg ctcaaaacgt cgctaaataa actctgttcc tttagatata tagacccgcc
     8401 gacataccct atataattac tattcttatc ataaatagga tgtgacaaaa aaataatcaa
     8461 attcccagaa gctgatttgt aaggtgccga tataaatggt tttttagcct caatcgccaa
     8521 cttactggca cttgaattca agtgaactcc aactaaacca agtgattctg gcgaagtcgc
     8581 cagaacaaca gcaccatcac taacaacaag aactgaatta aacataccag actgcaatct
     8641 taacctatcc gcctcatttt taagtgagtc aatatcatta gttgactcta ttttttttgc
     8701 gccatacgcc aattcacgtt gagcaatttc aatataatta ctcattacat ctgacatttt
     8761 catagcgtac gatgaattag catcaagcaa gtctttttct atattttcgc gtttaaaaac
     8821 cataataacg acaatcagaa aaatagaagt caacaaaata ccaccaacgg acaaggttat
     8881 gactatcgga cgaaggtgag gctttttaat tctcataaaa atacactcta tataattaaa
     8941 aaattccaca tttaccataa catcccttgc ccgcaccgcc ccggacgggt tcggcgcgaa
     9001 tgcggcgtca gcccggtaaa accggcagta aactccccgc taaaccgcgc tttttgagcc
     9061 agataaattt ccccaagatg catagccatg cagccggggg gatggcaccg cctttcggcg
     9121 gggatctgcg ccgaaagacc cgttttatcc ggtttgttac agcgacggac cgttgtacgt
     9181 ccgctggcgc tgcgatcgtt ccagctccag cgctttccgg tgctgcgccg cccgttcctg
     9241 ctcccggtgc gcctcgatac cattcagcct tgcgacaacc ggcgcagctg ctcgctcaaa
     9301 ctctgcacct gcctgctcaa gcccgtgacg cgctcgctca gcgccgcgtt ctcccgttgc
     9361 ataagaccag acatcgtccg ccattccgcg aaggcgttct cccactcgtc cagccttttc
     9421 gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gtttttccag ctccgtcatg
     9481 tgctatttcc ccgccagtat catccagctc gaatcctctc cgaaccgtcc cgcttccggg
     9541 ttcaccctca cgcagctgcg tctctgctcg ccgcaggtcg agagctgcac gccgctgttc
     9601 cgctcggaca gcatcgcctg cgtgctcttc tgctcccgga tggtcgtgta attgtcgagt
     9661 atctgctgac tctgccacca cagaatgccc gcgctcgatg cgatcagcag gacggaaacc
     9721 aggacgatgg tcagccacgt ctggctgacc atacgcacca tgcctttcgt gcgctggctc
     9781 atggctgagg acagcttccg gtcgtggtcg aggatggcgg cgctgattct cttctcgctt
     9841 tcgttcagtt ccgctctgac agactgctcg tgctcgctga atgcggcttt cagcatctcg
     9901 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
     9961 ctgctcatag atggctccct ttagtcggat gtttcgcccc ccgtccgggt cggcaatgct
    10021 gatactgctt ttcgtggtgc gtaccac
//
