LOCUS       NZ_CP065065            11078 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica strain RG21060838 plasmid
            p838-11k, complete sequence.
ACCESSION   NZ_CP065065
VERSION     NZ_CP065065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16807069
            Assembly: GCF_031036675.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica
  ORGANISM  Salmonella enterica subsp. enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Huang,T.-W.
  TITLE     Whole-genome sequencing of ceftriaxone-resistant Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Huang,T.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2020) Department of Microbiology and Immunology,
            School of Medicine, College of Medicine, Taipei Medical University,
            No. 250, Wuxin Street, Taipei 11031, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: HGAP v. JAN-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 330.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 16:19:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,929
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 51 of 157
            Pseudo Genes (incomplete)         :: 111 of 157
            Pseudo Genes (internal stop)      :: 28 of 157
            Pseudo Genes (multiple problems)  :: 29 of 157
            Pseudo Genes (short protein)      :: 1 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065065.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Salmonella enterica subsp. enterica"
                     /mol_type="genomic DNA"
                     /strain="RG21060838"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:59201"
                     /plasmid="p838-11k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2017-07"
     gene            101..915
                     /gene="sul2"
                     /locus_tag="I0P42_RS25185"
                     /old_locus_tag="I0P42_25030"
                     /pseudo=""
     ARG             102..892
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="98.39"
                     /coverage="97.05"
     ARG             1361..1779
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             977..1776
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1779..2615
                     /locus_tag="I0P42_RS25195"
                     /old_locus_tag="I0P42_25040"
     CDS             1779..2615
                     /locus_tag="I0P42_RS25195"
                     /old_locus_tag="I0P42_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3002..3244
                     /locus_tag="I0P42_RS25200"
                     /old_locus_tag="I0P42_25045"
     CDS             3002..3244
                     /locus_tag="I0P42_RS25200"
                     /old_locus_tag="I0P42_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(3286..3936)
                     /gene="tetR(A)"
                     /locus_tag="I0P42_RS25205"
                     /old_locus_tag="I0P42_25050"
     CDS             complement(3286..3936)
                     /gene="tetR(A)"
                     /locus_tag="I0P42_RS25205"
                     /old_locus_tag="I0P42_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_012850383.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHSGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4042..5241
                     /gene="tet(A)"
                     /locus_tag="I0P42_RS25210"
                     /old_locus_tag="I0P42_25055"
     ARG             4043..5238
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(5172..5612)
                     /locus_tag="I0P42_RS25215"
                     /old_locus_tag="I0P42_25060"
     CDS             complement(5172..5612)
                     /locus_tag="I0P42_RS25215"
                     /old_locus_tag="I0P42_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025732385.1"
                     /translation="MSSNGRHFGEIRLSALKKAPEMFGSVYEHEEKKPMEAFAERLRDA
                     VAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLLRAVIDYGRE
                     HVEQIMLTVAEGNEAAINLYQRSARCPAPLQSPRRSAGRQSK"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(6071..6355)
                     /locus_tag="I0P42_RS25220"
                     /old_locus_tag="I0P42_25065"
     CDS             complement(6071..6355)
                     /locus_tag="I0P42_RS25220"
                     /old_locus_tag="I0P42_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            6692..>7144
                     /locus_tag="I0P42_RS25225"
                     /pseudo=""
     CDS             6692..>7144
                     /locus_tag="I0P42_RS25225"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            7302..7715
                     /locus_tag="I0P42_RS25230"
     CDS             7302..7715
                     /locus_tag="I0P42_RS25230"
                     /inference="COORDINATES: protein motif:HMM:NF036929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            7814..8683
                     /locus_tag="I0P42_RS25235"
     CDS             7814..8683
                     /locus_tag="I0P42_RS25235"
                     /inference="COORDINATES: protein motif:HMM:NF028103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            8744..8956
                     /locus_tag="I0P42_RS25240"
                     /old_locus_tag="I0P42_25075"
     CDS             8744..8956
                     /locus_tag="I0P42_RS25240"
                     /old_locus_tag="I0P42_25075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8958..9164
                     /gene="urfF"
                     /locus_tag="I0P42_RS25245"
                     /old_locus_tag="I0P42_25080"
     CDS             8958..9164
                     /gene="urfF"
                     /locus_tag="I0P42_RS25245"
                     /old_locus_tag="I0P42_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            9194..10033
                     /locus_tag="I0P42_RS25250"
                     /old_locus_tag="I0P42_25085"
     CDS             9194..10033
                     /locus_tag="I0P42_RS25250"
                     /old_locus_tag="I0P42_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            10020..10871
                     /gene="repC"
                     /locus_tag="I0P42_RS25255"
                     /old_locus_tag="I0P42_25090"
     CDS             10020..10871
                     /gene="repC"
                     /locus_tag="I0P42_RS25255"
                     /old_locus_tag="I0P42_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     ARG             1780..2612
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          6310..6465
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          9212..10033
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          6555..8680
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctaatgga tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc
       61 cgtatgatac tcatgctcga ttattattat agaagccccc atgaataaat cgctcatcat
      121 tttcggcatc gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc
      181 agacgcagcc attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct
      241 cggtccggca tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg
      301 tatcgcgccg gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta
      361 tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg
      421 cggttttcca gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt
      481 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
      541 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
      601 acgcaaccgc cttgtccttg atcccggcat ggggtttttt ctggggctgc tcccgaaacc
      661 tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt
      721 ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc
      781 ggggccgcga cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc
      841 acacacgagc cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc
      901 gcaagaattc gttaactgca cattcgggat atttctctat attcgcgctt catcagaaaa
      961 ctgaaggaac ctccattgaa tcgaactaat attttttttg gtgaatcgca ttctgactgg
     1021 ttgcctgtca gaggcggaga atctggtgat tttgtttttc gacgtggtga cgggcatgcc
     1081 ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc
     1141 atttggctca aaggtcgagg tgtggcttgc cccgaggtca tcaactggca ggaggaacag
     1201 gagggtgcat gcttggtgat aacggcaatt ccgggagtac cggcggctga tctgtctgga
     1261 gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac ttggcgctgt tcacagccta
     1321 tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat
     1381 gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg acgaggacaa gagtacgccg
     1441 cagctcgatc ttttggctcg tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc
     1501 accgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt ggaccctaaa
     1561 actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga tcgctatgcc
     1621 gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga tgaagcagag
     1681 cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg cgaacgcctt
     1741 gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg cctgtttttc
     1801 ctgctcattg gcacgtttcg caacctgttc tcattgcgga caccttttcc agcctcgttt
     1861 ggaaagtttc attgccagac gggactcctg caatcgtcaa gggattgaaa cctatagaag
     1921 acattgctga tgaactgcgc ggggccgact atctggtatg gcgcaatggg aggggagcag
     1981 tccggttgct cggtcgtgag aacaatctga tgttgctcga atatgccggg gagcgaatgc
     2041 tctctcacat cgttgccgag cacggcgact accaggcgac cgaaattgca gcggaactaa
     2101 tggcgaagct gtatgccgca tctgaggaac ccctgccttc tgcccttctc ccgatccggg
     2161 atcgctttgc agctttgttt cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg
     2221 actacgtcca cgcggcgatt atagccgatc aaatgatgag caatgcctcg gaactgcgtg
     2281 ggctacatgg cgatctgcat catgaaaaca tcatgttctc cagtcgcggc tggctggtga
     2341 tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc
     2401 cggctgacag agacgacctt tgtctcgatc ctagacgcat tgcacagatg gcggacgcat
     2461 tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt
     2521 gcctttccgc agcttggaac gcggatggag aagaggagca acgcgatcta gctatcgcgg
     2581 ccgcgatcaa gcaggtgcga cagacgtcat actagatatc aagcgacttc tcctatcccc
     2641 tgggaacaca tcaatctcac cggagaatat cgctggccaa agccttagcg taggattccg
     2701 ccccttcccg caaacgaccc ctttctggcg cgtttgcagg gccatagacg gccgccagcc
     2761 cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctggaagcc ccgtagcgac
     2821 gcggagaggg gcgagacaag ccaagggcgc aggctcgatg cgcagcacga catagccggt
     2881 tctcgcaagg acgagaattt ccctgcggtg cccctcaagt gtcaatgaaa gtttccaacg
     2941 cgagccattc gcgagagcct tgagtccacg ctagatctat ctcatctgcg caaggcagaa
     3001 cgtgaagacg gccgccctgg acctcgcccg cgagcgccag gcgcacgagg ccggcgcgcg
     3061 gacccgcgcc acggcccacg agcggacgcc gcagcaggag cgccagaagg ccgccagaga
     3121 ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc ccgtagcggg
     3181 ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca tggctctgct
     3241 gtagtgagtg ggttgcgctc cggcagcggt cctgagcggt cctgatcaat cgtcaccctt
     3301 tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac
     3361 cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc
     3421 ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct
     3481 gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc
     3541 attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc
     3601 cgtttccatc tgcggtgcgc ccggtcgcgt gccggaatgg atgcgcgcgc catcgcggta
     3661 ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc
     3721 gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc
     3781 gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg
     3841 ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc
     3901 ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa
     3961 tataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg
     4021 aggctggtcc ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact
     4081 gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc
     4141 gatctggttc actcgaacga cgtcgccgcc cactatggca ttctgctggc gctgtatgcg
     4201 ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg
     4261 ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg
     4321 cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg
     4381 gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc
     4441 ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg
     4501 atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat
     4561 ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc
     4621 cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc
     4681 gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg
     4741 gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc
     4801 gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg
     4861 ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg
     4921 cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt
     4981 ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag
     5041 gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc
     5101 ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca
     5161 ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca
     5221 gggcaacgag ccgatcgctg atagaggttt atagcggctt cgttcccttc cgcaaccgtc
     5281 agcatgatct gctccacatg ctcgcggcca tagtctatca ccgcccgcag cagcataccg
     5341 gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga
     5401 gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg
     5461 taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc
     5521 tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg
     5581 atctcgccga aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccgtac
     5641 taactgtcac gaacccctgc aataactgtc acgcccccct gcaataactg tcacgaaccc
     5701 ctgcaataac tgtcacgccc ccaaacctgc aaacccagca ggggcggggg ctggcggggt
     5761 gttggaaaaa tccatccatg attatctaag aataatccac taggcgcggt tatcagcgcc
     5821 cttgtggggc gctgctgccc ttgcccaata tgcccggcca gaggccggat agctggtcta
     5881 ttcgctgcgc taggctacac accgccccac cgctgcgcgg cagggggaaa ggcgggcaaa
     5941 gcccgctaaa ccccacacca aaccccgcag aaatacgctg gagcgctttt agccgcttta
     6001 gcggcctttc cccctacccg aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc
     6061 ggtcgatcat tcagcccggc tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt
     6121 cgtggtcgcg ttcaaggtac gcatccattg ccgccatgag ccgatcctcc ggccactcgc
     6181 tgctgttcac cttggccaaa atcatggccc ccaccagcac cttgcgcctt gtttcgttct
     6241 tgcgctcttg ctgctgttcc cttgcccgca cccgctgaat ttcggcattg attcgcgctc
     6301 gttgttcttc gagcttggcc agccgatccg ccgccttgtt gctcccctta accatcttga
     6361 caccccattg ttaatgtgct gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc
     6421 gaccctactt tgtaggggag ggcgcactta ccggtttctc ttcgagaaac tggcctaacg
     6481 gccacccttc gggcggtgcg ctctccgagg gccattgcat ggagccgaaa agcaaaagca
     6541 acagcgaggc agcatggcga tttatcacct tacggcgaaa accggcagca ggtcgggcgg
     6601 ccaatcggcc agggccaagg ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat
     6661 ggatgaagtc ttgcacgccg aatccgggca catgccggag ttcgtcgagc ggcccgccga
     6721 ctactgggat gctgccgacc tgtatgaacg cgccaatggg cggctgttca aggaggtcga
     6781 atttgccctg ccggtcgagc tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc
     6841 ccagcacctg actggtgccg agcgcctgcc gtatacgctg gccatccatg ccggtggcgg
     6901 cgagaacccg cactgccacc tgatgatctc cgagcggatc aatgacggca tcgagcggcc
     6961 cgccgctcag tggttcaagc ggtacaacgg caagaccccg gagaagggcg gggcacagaa
     7021 gaccgaagcg ctcaagccca aggcatggct tgagcagacc cgcgaggcat gggccgacca
     7081 tgccaaccgg gcattagagc gggctggcca cgacgcccgc attgaccaca gaacacttga
     7141 ggcgcagggc atcgagcgcc tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga
     7201 aggccggggc atccgcaccg accgggcaga cgtggccctg aacatcgaca ccgccaacgc
     7261 ccagatcatc gacttacagg aataccggga ggcaatagac catgaacgca atcgacagag
     7321 tgaagaaatc cagaggcatc aacgagttag cggagcagat cgaaccgctg gcccagagca
     7381 tggcgacact ggccgacgaa gcccggcagg tcatgagcca gacccagcag gccagcgagg
     7441 cgcaggcggc ggagtggctg aaagcccagc gccagacagg ggcggcatgg gtggagctgg
     7501 ccaaagagtt gcgggaggta gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg
     7561 cgtcgcgggg gtggcactgg aagctatggc taaccgtgat gctggcttcc atgatgccta
     7621 cggtggtgct gctgatcgca tcgttgctct tgctcgacct gacgccactg acaaccgagg
     7681 acggctcgat ctggctgcgc ttggtggccc gatgaagaac gacaggactt tgcaggccat
     7741 aggccgacag ctcaaggcca tgggctgtga gcgcttcgat atcggcgtca gggacgccac
     7801 caccggccag atgatgaacc gggaatggtc agccgccgaa gtgctccaga acacgccatg
     7861 gctcaagcgg atgaatgccc agggcaatga cgtgtatatc aggcccgccg agcaggagcg
     7921 gcatggtctg gtgctggtgg acgacctcag cgagtttgac ctggatgaca tgaaagccga
     7981 gggccgggag cctgccctgg tagtggaaac cagcccgaag aactatcagg catgggtcaa
     8041 ggtggccgac gccgcaggcg gtgaacttcg ggggcagatt gcccggacgc tggccagcga
     8101 gtacgacgcc gacccggcca gcgccgacag ccgccactat ggccgcttgg cgggcttcac
     8161 caaccgcaag gacaagcaca ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga
     8221 atccaagggc aagaccgcca ccgctggccc ggcgctggtg cagcaggctg gccagcagat
     8281 cgagcaggcc cagcggcagc aggagaaggc ccgcaggctg gccagcctcg aactgcccga
     8341 gcggcagctt agccgccacc ggcgcacggc gctggacgag taccgcagcg agatggccgg
     8401 gctggtcaag cgcttcggtg atgacctcag caagtgcgac tttatcgccg cgcagaagct
     8461 ggccagccgg ggccgcagtg ccgaggaaat cggcaaggcc atggccgagg ccagcccagc
     8521 gctggcagag cgcaagcccg gccacgaagc ggattacatc gagcgcaccg tcagcaaggt
     8581 catgggtctg cccagcgtcc agcttgcgcg ggccgagctg gcacgggcac cggcaccccg
     8641 ccagcgaggc atggacaggg gcgggccaga tttcagcatg tagtgcttgc gttggtactc
     8701 acgcctgtta tactatgagt actcacgcac agaagggggt tttatggaat acgaaaaaag
     8761 cgcttcaggg tcggtctacc tgatcaaaag tgacaagggc tattggttgc ccggtggctt
     8821 tggttatacg tcaaacaagg ccgaggctgg ccgcttttca gtcgctgata tggccagcct
     8881 taaccttgac ggctgcacct tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa
     8941 gtttctcggt gactgatatg aaagaccaaa aggacaagca gaccggcgac ctgctggcca
     9001 gccctgacgc tgtacgccaa gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc
     9061 agcgcaagtt ctggctgacc gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac
     9121 tcagagcggc gcagggcggg ggtagtgacc ccgccagcgc ctaaccacca actgcctgca
     9181 aaggaggcaa tcaatggcta cccataagcc tatcaatatt ctggaggcgt tcgcagcagc
     9241 gccgccaccg ctggactacg ttttgcccaa catggtggcc ggtacggtcg gggcgctggt
     9301 gtcgcccggt ggtgccggta aatccatgct ggccctgcaa ctggccgcac agattgcagg
     9361 cgggccggat ctgctggagg tgggcgaact gcccaccggc ccggtgatct acctgcccgc
     9421 cgaagacccg cccaccgcca ttcatcaccg cctgcacgcc cttggggcgc acctcagcgc
     9481 cgaggaacgg caagccgtgg ctgacggcct gctgatccag ccgctgatcg gcagcctgcc
     9541 caacatcatg gccccggagt ggttcgacgg cctcaagcgc gccgccgagg gccgccgcct
     9601 gatggtgctg gacacgctgc gccggttcca catcgaggaa gaaaacgcca gcggccccat
     9661 ggcccaggtc atcggtcgca tggaggccat cgccgccgat accgggtgct ctatcgtgtt
     9721 cctgcaccat gccagcaagg gcgcggccat gatgggcgca ggcgaccagc agcaggccag
     9781 ccggggcagc tcggtactgg tcgataacat ccgctggcag tcctacctgt cgagcatgac
     9841 cagcgccgag gccgaggaat ggggtgtgga cgacgaccag cgccggttct tcgtccgctt
     9901 cggtgtgagc aaggccaact atggcgcacc gttcgctgat cggtggttca ggcggcatga
     9961 cggcggggtg ctcaagcccg ccgtgctgga gaggcagcgc aagagcaagg gggtgccccg
    10021 tggtgaagcc taagaacaag cacagcctca gccacgtccg gcacgacccg gcgcactgtc
    10081 tggcccccgg cctgttccgt gccctcaagc ggggcgagcg caagcgcagc aagctggacg
    10141 tgacgtatga ctacggcgac ggcaagcgga tcgagttcag cggcccggag ccgctgggcg
    10201 ctgatgatct gcgcatcctg caagggctgg tggccatggc tgggcctaat ggcctagtgc
    10261 ttggcccgga acccaagacc gaaggcggac ggcagctccg gctgttcctg gaacccaagt
    10321 gggaggccgt caccgctgat gccatggtgg tcaaaggtag ctatcgggcg ctggcaaagg
    10381 aaatcggggc agaggtcgat agtggtgggg cgctcaagca catacaggac tgcatcgagc
    10441 gcctttggaa ggtatccatc atcgcccaga atggccgcaa gcggcagggg tttcggctgc
    10501 tgtcggagta cgccagcgac gaggcggacg ggcgcctgta cgtggccctg aaccccttga
    10561 tcgcgcaggc cgtcatgggt ggcggccagc atgtgcgcat cagcatggac gaggtgcggg
    10621 cgctggacag cgaaaccgcc cgcctgctgc accagcggct gtgtggctgg atcgaccccg
    10681 gcaaaaccgg caaggcttcc atagatacct tgtgcggcta tgtctggccg tcagaggcca
    10741 gtggttcgac catgcgcaag cgccgccagc gggtgcgcga ggcgttgccg gagctggtcg
    10801 cgctgggctg gacggtaacc gagttcgcgg cgggcaagta cgacatcacc cggcccaagg
    10861 cggcaggctg accccccccc actctattgt aaacaagaca tttttatctt ttatattcaa
    10921 tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag aaaaggctta
    10981 acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg ccgctttcct
    11041 ggctttgctt cccagatgta tgctcttctg ctcctgca
//
