LOCUS       NZ_CP064850             2101 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain 20 plasmid pHK01-3, complete sequence.
ACCESSION   NZ_CP064850
VERSION     NZ_CP064850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16520638
            Assembly: GCF_015649805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Jovanovic,O. and Shang,C.
  TITLE     Inactivation mechanism and efficacy of UV-LEDs at 265, 285 and 300
            nm for inactivation of ESBL-E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Jovanovic,O., Amabile-Cuevas,C.F. and Shang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2020) CIVL, The HKUST, Clear Water Bay, Hong Kong
            999077, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: FALCON v. NA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 13:47:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,168
            CDSs (total)                      :: 5,051
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 57 of 158
            Pseudo Genes (incomplete)         :: 121 of 158
            Pseudo Genes (internal stop)      :: 24 of 158
            Pseudo Genes (multiple problems)  :: 39 of 158
            Pseudo Genes (short protein)      :: 4 of 158
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064850.1.
            Bacteria and source DNA available from The HKUST, Clear Water Bay;
            prof. Chii SHANG; Civil and Environmental Engineering.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20"
                     /serovar="O11:H25"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pHK01-3"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2018-06"
                     /genotype="Strain is confirmed ESBL producer resistant to
                     CTX, CAZ, SXT, RL (EUCAST guidelines)"
     gene            590..1555
                     /locus_tag="IPT86_RS25630"
                     /old_locus_tag="IPT86_25630"
     CDS             590..1555
                     /locus_tag="IPT86_RS25630"
                     /old_locus_tag="IPT86_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1559..1825
                     /locus_tag="IPT86_RS25635"
                     /old_locus_tag="IPT86_25635"
     CDS             1559..1825
                     /locus_tag="IPT86_RS25635"
                     /old_locus_tag="IPT86_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          984..1215
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat ctccgagagt
       61 taatctcgtc aaatgctaaa tcgtgggggt cccctttggg gttccgattt agtgattgac
      121 gacaccaccg attaaaaaac ttatgcgggg tggatggttt cacgaagtga ggccatccac
      181 ctgtaagaca gggttttgtt tttattccct gttttagtga tcgggtgtgt ggaaaaggtt
      241 ggggtaagcc gttcgggggt gcttgttttg gggggttaaa attgtggtta ttttttgcgc
      301 aattctcgcg cgtgatcctt gtatttatac ttaagggata aatggcggat atgaaatagt
      361 ggtttagccc agtaatgacg aggctttgag tgggttttga caggtcaaag aaaatggagc
      421 agaattgagg cgtttttaat cggcgttggg gagtgcgtca acactcccca acatttcgaa
      481 tgtgtcacct cagcggcaaa ctctggtgac atgtactggc tcgcaatgca caggtacgtg
      541 atgaatatac cacatcaaat cacagcctgc ccagatcgga gcaggcttaa tgtcagaaga
      601 taaattcctt tcggactaca gcccccgtga tgcagtttgg gatacccagc gcacgcttac
      661 cgattctgtc gggggtatct accagactgc tgctgaattc gagcgctatg cactccgtat
      721 ggcctcctgt agcggtttgt tacgttttgg ttggtctacc atcatggaaa ccggagaaac
      781 gcgcctacgg cttcgtagtg cgcaattttg ccgtgtccgt cattgccctg tctgccagtg
      841 gagaagaacc ctcatgtggc aagcccgttt ttatcaggct ctaccgaaaa tcgttgtgga
      901 ttacccgtct tcccgatggt tgtttctgac gttaactgtc aggaactgcg agataggtga
      961 acttggaaca gtccttacag caatgaatgc ggcgtttaag cgaatggaaa agcgaaagga
     1021 gctatcacct gttcaggggt ggatcagggc tacggaggtg acgcgaggta aggatggcag
     1081 cgcacatccg cattttcact gtctgctgat ggtgcaacct tcttggttta aagggaagaa
     1141 ctacgttaag cacgaacgtt gggtagaact ctggcgcgat tgcttgcggg tgaactatga
     1201 gccgaatatc gatattcggg cagtaaaaac taagacaggt gaggttgtgg ccaacgttgc
     1261 cgagcaactg caaagcgcgg ttgctgaaac gctgaaatac tccgttaaac cggaagatat
     1321 ggcaaacgat cctgagtggt ttcttgagct gacgcggcag cttcacaagc gccgttttat
     1381 ctcgaccggt ggggcgctaa aaaacgtcct ccagttggat cgagaaacca atgaggatct
     1441 tgtcattgcc gacgatgtag gggatggcac tgatgacggg aagcggacgg cgtttgtctg
     1501 ggattcaggt aaacggcgtt acaaacgcgc ccctgagaag gataaatcgg attaacgtat
     1561 gaatattaat attgaatacc tgaatggaaa taagactatt ggtttatttt ttttaagaag
     1621 tgaagcggtg attcctgaca ggtttaaaaa ccttattttg cttattgatg gattaagttt
     1681 tggcacattt ggttttcatc cgcacgaagg ttttgaggat gaattaattt tatatattca
     1741 gaaaacaaac gagagggtaa aaactctttt tgtgaaaata gatttaaacg gtatcaagtt
     1801 tggattttta agaacgcatt cttagttctg gaaaagagcc agcggcaggc tgaggtgata
     1861 ggtacgagat tgcatgcaat ctctagtgct ctgtctatcc tgcattatcc tcagcattat
     1921 cctcagcatt atcctcagcc ttgccaactc gacaccaatg caggatagac aatccgatgt
     1981 caaatgttaa cactctgcga gtggtacatt ttccccggat tatcgtcctg agcctgccgc
     2041 tggctctctt tctaccgcct cgctttgctc gttgctcaac gcctcacaga cacggattaa
     2101 a
//
