LOCUS       NZ_CP064849             5166 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain 20 plasmid pHK01-2, complete sequence.
ACCESSION   NZ_CP064849
VERSION     NZ_CP064849.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16520638
            Assembly: GCF_015649805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Jovanovic,O. and Shang,C.
  TITLE     Inactivation mechanism and efficacy of UV-LEDs at 265, 285 and 300
            nm for inactivation of ESBL-E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Jovanovic,O., Amabile-Cuevas,C.F. and Shang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2020) CIVL, The HKUST, Clear Water Bay, Hong Kong
            999077, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: FALCON v. NA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 13:47:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,168
            CDSs (total)                      :: 5,051
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 57 of 158
            Pseudo Genes (incomplete)         :: 121 of 158
            Pseudo Genes (internal stop)      :: 24 of 158
            Pseudo Genes (multiple problems)  :: 39 of 158
            Pseudo Genes (short protein)      :: 4 of 158
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064849.1.
            Bacteria and source DNA available from The HKUST, Clear Water Bay;
            prof. Chii SHANG; Civil and Environmental Engineering.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20"
                     /serovar="O11:H25"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pHK01-2"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2018-06"
                     /genotype="Strain is confirmed ESBL producer resistant to
                     CTX, CAZ, SXT, RL (EUCAST guidelines)"
     gene            1..915
                     /locus_tag="IPT86_RS25605"
                     /old_locus_tag="IPT86_25605"
     CDS             1..915
                     /locus_tag="IPT86_RS25605"
                     /old_locus_tag="IPT86_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1119..1517)
                     /locus_tag="IPT86_RS25610"
                     /old_locus_tag="IPT86_25610"
     CDS             complement(1119..1517)
                     /locus_tag="IPT86_RS25610"
                     /old_locus_tag="IPT86_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1694..2620
                     /locus_tag="IPT86_RS25615"
                     /old_locus_tag="IPT86_25615"
     CDS             1694..2620
                     /locus_tag="IPT86_RS25615"
                     /old_locus_tag="IPT86_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_097509673.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERYVWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGLD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2623..3156
                     /locus_tag="IPT86_RS25620"
                     /old_locus_tag="IPT86_25620"
     CDS             2623..3156
                     /locus_tag="IPT86_RS25620"
                     /old_locus_tag="IPT86_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3222..3431)
                     /locus_tag="IPT86_RS25625"
                     /old_locus_tag="IPT86_25625"
     CDS             complement(3222..3431)
                     /locus_tag="IPT86_RS25625"
                     /old_locus_tag="IPT86_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106106857.1"
                     /translation="MAKLGENPPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          2..776
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1695..2617
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.377"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc atcgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacata
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag
      601 tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt
      661 tcactggctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgcggtcgca agggcggttc taagtctaag cgcagcacag tagctacatc tgcacgcacg
      841 ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttc tggcgtcttt
      961 ttgagcagca ggttttttgc cgtttttccc cattcagccc tgcaatggcg cggctttccg
     1021 cgtgattgtg gtggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcac cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat
     1141 gtctccagat cgtacttaag ttccttaagt gcctgtaata tcctgtcttt tttatagcat
     1201 agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag
     1261 agccggtgat tgcgttcccg tcgttgtttt tttccctcga ttcgtttctg gaccgcctgt
     1321 tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggaga
     1381 agtttgcgtt cctgttctgt gagtccggct tcgttgtcga tcaggtcaat aatctgtctc
     1441 tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg
     1501 atgttgctga tgcgcatgtt cgtgatgctc cctggtgttt tttgcggtgt gttgtggtcg
     1561 tgcagtatat cgcttgcggt cggtgggtgc aggggggtat cttccgtgct aattctaaaa
     1621 attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg
     1681 gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggtggca aggggcaggc
     1741 tgcaaaccac gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc aggatttgga
     1801 atattccgca cacggaaaca tgccggaatg ggccagggat aatccttccc atttctggca
     1861 ggcggctgac cagtttgaac gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct
     1921 gccccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga
     1981 agccggcgaa cggtacgtct ggagttttgc cattcacaat ccgaaagcga gcattgacgg
     2041 cggcgaacag cctcatgcgc atatcatgat gagccagcgt gtgaatgatg gcatagaacg
     2101 tactccggaa cagtatttcc ggcgctataa cgccaggtat cccgaccggg gaggtgcgaa
     2161 aaaggattcc ggaagcctga ctcccactca gcaaaaagag cagttgcggg aattgcgaaa
     2221 acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa ggggcttcat
     2281 tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc atctgggttt
     2341 tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa aacggtctga
     2401 accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca tcagggaatg
     2461 gctggacact ccggttaagc aacatgaact ggtcagaagg cttcaacatg atgttgcgcg
     2521 ggagcatggc cttgacatga gagacagagt gagcagacag gaccgaactg aacctgaacg
     2581 ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag cacagaagaa
     2641 aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat ggagcaacag
     2701 aaagctgttg ctgattctct ggctgtggca agggaaacac ttcgcggata tcgggatatt
     2761 tctgacggcg tggcaaaaca ggtcagggat gaagtccgaa atgaggtgca gaagctggat
     2821 gtggcagggc tgataggtga gcgcatcagc caggattttc agcggctgga agagtcagtc
     2881 gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat tcacagcaat
     2941 atcgtacagg aatacaacac ccttaaaggg tatagctgga aatggctggg tggggtgtca
     3001 gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat actcgacagg
     3061 aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga taaactggtg
     3121 gagaaacctg tcacggggaa gagccggaaa aactgacgcg cttcgcttgt ccaacgcctc
     3181 acgacccgcg aaaggatttc gcgaaccgtt caccgttttt ttcaatatga ttctgtctgc
     3241 tttggtcgat aaagcagacg atagtattcc agccttcgta tatataacgg tacgtgctca
     3301 tcagtaagag ctggcggtat tgtgttatca gggggaagtt ttttcagata ctcacgaaat
     3361 gcctggcttg atgccataaa atctacagta gcatcgataa tatgtggggg gttttcacct
     3421 aattttgcca tgatgataat ccttatatta tcacggtttg tgaatgatag ggtgtcacta
     3481 ttatttatgc gttagtgaac cagaaaaaat cccccgttgg cataggggga tatgtaagga
     3541 gaaataactt cgcaggtgtt cttgtttgta taacgtgaaa agactgcacg caacttaata
     3601 agtctgccgt acttactttc agttttcaac ataaggttta taacaatgta tggaatggaa
     3661 gtgagcttta aggtttcagc tctaaaaaat aaaatattta ttagttggct ggttgttgtt
     3721 cttgttctgt atgtatataa acactatcga catattatga ccgggaacat tccttacccc
     3781 ctctgcgctc gcagaggttt tttttgtcat taattaataa tgaactgaaa tgcagttccg
     3841 atacctccat ttgcacatta tccctgacat atggtaccgg aaccgcatta attttatgtt
     3901 ataagagttt atttttaatg tccagatggt ttaatagtat ttatatgtaa ttagctgtat
     3961 ggatagtact ggttttattg tataacttgt cgtttttttt attgtttttg tgtggtgcgc
     4021 taatctttaa gttataaaaa aaccccttgg aatacagggg caaaagtaag gaaaacaaat
     4081 ggatgctcag tttgtgtgac actttaaact aatggaacta tagagtggag gttgttacag
     4141 tcgcttttat aaaacagcaa ccagtttttt ttcaatagtc tgatgatggt tgtgacagtt
     4201 ttgtgatatt gttcaaaatg tttcttgttg aatgtttgtt ttttggtcta ttggtgtctg
     4261 gttttatttt ttctgctatt acatatcaag aaatgtgatt ggtgtagcaa gagtggaatg
     4321 cgatgtggat aaactaattg gttgtcgaat ctaatttact tagaaaacta cacttgtttt
     4381 tgtgtatgaa tttagtgaat ttgtctgtct atgtgttttc gtgcatttat aaatccttcg
     4441 tcaggatctt aaatttagtg ttgaatatgt agggcctgta cggtaattat tatttagcgt
     4501 agctacttca tcgttgttcg aacacaacca acaacctgta attgttgttt ctcttggttc
     4561 cccacttttg tggggttttt tatctcttcc gtgcatcgta aagcgcggac tccttctgga
     4621 aaggttgttt tagggcatca ctgaggcatt ttttgcgttt gtgaatattg agcatccgat
     4681 aactaaaata tttcccatag actctgattc ttgccaccga ccccaggtcg gttttttttg
     4741 tcctggcgag atgtgaagtt tggaaacagg aaaccccgtc agagcgccac gcaccagggg
     4801 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg
     4861 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
     4921 ctggtgtttc cttgtctttt tgtgtttttt gcgtcagaaa gcgactgagg gcgttttaat
     4981 gggtacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
     5041 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
     5101 ccggcaagat tcaacatgaa atacagaggg cgtgtaggtc gcataacccg gagagaatcg
     5161 taacac
//
