LOCUS       NZ_CP064429             9316 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain PR01996-12 plasmid unnamed_5, complete
            sequence.
ACCESSION   NZ_CP064429
VERSION     NZ_CP064429.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16327730
            Assembly: GCF_018219325.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Kinnear,C.L., Hansen,E., Morley,V.J., Tracy,K.C., Forstchen,M.,
            Read,A.F. and Woods,R.J.
  TITLE     Daptomycin treatment impacts resistance in off-target populations of
            vancomycin-resistant Enterococcus faecium
  JOURNAL   PLoS Biol 18 (12), e3000987 (2020)
   PUBMED   33332354
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Morley,V.J., Tracy,K.C., Kinnear,C.L. and Woods,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2020) Computational Medicine and Bioinformatics,
            University of Michigan, Palmer Commons, 100 Washtenaw Ave #2017, Ann
            Arbor, MI 48104, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57x
            Sequencing Technology :: Illumina NovaSeq + Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018219325.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:20:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,854
            CDSs (with protein)               :: 2,854
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 6 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 134 of 209
            Pseudo Genes (incomplete)         :: 66 of 209
            Pseudo Genes (internal stop)      :: 36 of 209
            Pseudo Genes (multiple problems)  :: 23 of 209
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064429.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="PR01996-12"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed_5"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.28 N 83.72 W"
                     /collection_date="2016"
                     /collected_by="University of Michigan"
     gene            complement(join(9067..9316,1..371))
                     /locus_tag="IUH71_RS15480"
                     /old_locus_tag="IUH71_15480"
     CDS             complement(join(9067..9316,1..371))
                     /locus_tag="IUH71_RS15480"
                     /old_locus_tag="IUH71_15480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(368..2494)
                     /locus_tag="IUH71_RS15485"
                     /old_locus_tag="IUH71_15485"
     CDS             complement(368..2494)
                     /locus_tag="IUH71_RS15485"
                     /old_locus_tag="IUH71_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(2548..2922)
                     /locus_tag="IUH71_RS15490"
                     /old_locus_tag="IUH71_15490"
     CDS             complement(2548..2922)
                     /locus_tag="IUH71_RS15490"
                     /old_locus_tag="IUH71_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3055..3516
                     /locus_tag="IUH71_RS15495"
                     /old_locus_tag="IUH71_15495"
     CDS             3055..3516
                     /locus_tag="IUH71_RS15495"
                     /old_locus_tag="IUH71_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3828..3998)
                     /locus_tag="IUH71_RS15500"
                     /old_locus_tag="IUH71_15500"
     CDS             complement(3828..3998)
                     /locus_tag="IUH71_RS15500"
                     /old_locus_tag="IUH71_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4014..4553)
                     /locus_tag="IUH71_RS15505"
                     /old_locus_tag="IUH71_15505"
     CDS             complement(4014..4553)
                     /locus_tag="IUH71_RS15505"
                     /old_locus_tag="IUH71_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4581..5318)
                     /locus_tag="IUH71_RS15510"
                     /old_locus_tag="IUH71_15510"
     CDS             complement(4581..5318)
                     /locus_tag="IUH71_RS15510"
                     /old_locus_tag="IUH71_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            5963..6049
                     /locus_tag="IUH71_RS15515"
                     /old_locus_tag="IUH71_15515"
     CDS             5963..6049
                     /locus_tag="IUH71_RS15515"
                     /old_locus_tag="IUH71_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6169..6858)
                     /locus_tag="IUH71_RS15520"
                     /old_locus_tag="IUH71_15520"
     CDS             complement(6169..6858)
                     /locus_tag="IUH71_RS15520"
                     /old_locus_tag="IUH71_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6860..7138)
                     /locus_tag="IUH71_RS16050"
     CDS             complement(6860..7138)
                     /locus_tag="IUH71_RS16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(7098..8012)
                     /locus_tag="IUH71_RS15525"
                     /old_locus_tag="IUH71_15525"
     CDS             complement(7098..8012)
                     /locus_tag="IUH71_RS15525"
                     /old_locus_tag="IUH71_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7994..8374)
                     /gene="mobC"
                     /locus_tag="IUH71_RS15530"
                     /old_locus_tag="IUH71_15530"
     CDS             complement(7994..8374)
                     /gene="mobC"
                     /locus_tag="IUH71_RS15530"
                     /old_locus_tag="IUH71_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(8816..8953)
                     /locus_tag="IUH71_RS15535"
                     /old_locus_tag="IUH71_15535"
     CDS             complement(8816..8953)
                     /locus_tag="IUH71_RS15535"
                     /old_locus_tag="IUH71_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     BGC             1..7922
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4582..5318)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7102..8012)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttggcaaag aaaaatctgt taataaatta taaatatgat cattggaaat attactgtta
       61 aacttttttg ctaattcgaa attgtattgt actgtttgat tttcaagaag agcatagttt
      121 tgaaataaat agctaatatg atttcttcgt agacgttgta tatcatgttt agaaactttc
      181 ttttcaaaat agaataagtt accggtatat tgtttatcta ttaggcttaa attatttaac
      241 agagtagttt ttccactacc actttttcct gttactataa taaaatcttt ttctgatatt
      301 gaaaagttcc aattttgttt gatctttttt ttattaattg acagggtgaa attctcaact
      361 gataatatca taaaatattc tccttaaaat atttaaaact atttttttta taaataataa
      421 tagaagtaat tagatttatt aaaaagatca taaaaattat aaaatacaag taagtgtttt
      481 ttgaaaatat caattgaaaa cacatagtag caaataactc tattgtaaaa gggagtaaac
      541 ataaatctaa aacttgactt attggtgaat atccaaatat tttttttaaa gctatttttt
      601 ttgaatttat agtaaaacta acaaaaatca tttgaaaatt aattattata aataatccta
      661 atattaaaag atattgtaga tatgcattta atttatttac ttttttttga gataataatt
      721 caccagtttc ttgaaaaaca gaataagaaa aatttatgat attttctagg ttaaattctt
      781 ttatatacgg attaatttga ttaaaaaagt tttggtttga agtatgtttc tgatcaaaaa
      841 ataatccacc atcactagtc aatatatttc cccaaaataa tgattctgtt atttctggat
      901 taataactct aactatggga tcagttattg tatagttatc ttttgccttt ccatataagg
      961 tattaaaact aaaatacttt tggttattat taacaaatat tagttttatt tttaaatttt
     1021 tcaaattagg atttttataa ggagcttgag gaggagagtc ctcaagtcta aactgtatgt
     1081 cttctaaata atttttttca atcatttttg catattcttt atatttttta ggtactagaa
     1141 tgtaagtagt tagtggatca gagtttatta aatttaagtc agtagattct tcaaaagaaa
     1201 taggattgta atattttaga tagtttaaat caacacatat tcctgcacca gcaggttgac
     1261 taacatagtc ttcaaaattt ggataaactt tttgtccaat aaaaaattct ttattattct
     1321 cttggatagt tgcgtaattg tatggcgcga taataaaagt tttataattc ttctgtattt
     1381 tataaacaaa tttactagct tttttcaagt agttattttc ttctacggtg ttgtttctat
     1441 ttaattgatt agtaatattt gttttgtata aattagtagt tctttcccaa tatttaagag
     1501 aattattttc ggcttcatta tgggtattta ttacttttgt agaagctatt aacagaagga
     1561 tcatgcttaa gaatagacag atatttaata tacaaagatt taacttaagt ggtcgtgaat
     1621 tttttagata attttttata ctagataaat gatagatact tagagtaact atgataaata
     1681 aaaaactaat tataaaatga aaaatactac atagaagaaa tgataggaaa aaattttcgt
     1741 tactgtaata tatgctcaaa aaaatagata aaatgaaagt tataatcaaa gaacaaaata
     1801 agaggtttaa ctctttaata gaatctttta tagtatcaaa agagttatag cctaacattt
     1861 tttttagagt gatttctttc atttttgaaa aagcaaatat aattgtagta attacaataa
     1921 atatactaag atagcagata acaagaacaa tattagtatt aatttgaaaa gtatctgatt
     1981 gattgatatt ggaaatagta ggtgttccat attttttcag tacaggaatt aatttgtttg
     2041 agtttccttg tacatataat atttcactta atccaatatt ttccacatga gaaaaaggat
     2101 aaatatttat atttaaaaaa ttatttggaa gttttaattg gtacttagaa tttttgttat
     2161 tagtataatg gactttagaa tcgtttcttg ctaatatatt ttttttagaa aatttccagt
     2221 gtttgttagc gttagggttt gtagcaagaa tagttagttg gttaacagca gtaaaactat
     2281 attgagaaat attaatattt tcttgtgtag atatttcata caaagcgttt atataactgt
     2341 taaagtccat atcatcttta tattgcatat gaatttcagc ggtattttta tatagcgctt
     2401 cagaagtctt tataccattt ataacattag tagaaaaaaa tagtattgta atgcagacaa
     2461 aaagtgataa aacagcgttt atttttttta acatattttg aatttccttt caaatgtaaa
     2521 taaaaaagag agtttaaact ctctttttta ataaacattc caccaaccag tatttccagt
     2581 aagtgtggca ttgcatgttg cataagctct tactccagct ttctgaactg atcgtacttg
     2641 accgcctcgt ccatttttag ctgttgcact atgtttagct ctagcatggt agtaatttgc
     2701 ataaacttta aatccattaa ccccacagtt gaaatccccg ccgccagcag catatctcaa
     2761 tttactagaa gaaaatcctt cttctgtagc ttccattcct cctccgtaaa tggcggtatg
     2821 gtcttcgggt acatctagat ttaaagcttc tgtggcacta gctattcctc cactagttaa
     2881 taagacaccc attaaagcag aaagacataa aaattttttc ataacttatt tctcctatct
     2941 tttttactag aattatttta ttttagtgtg ttttataaaa taaagaactt ttttggcgcg
     3001 ttagtatttt tatgataaaa ttttatgtaa ataatcgttt acataggaga aaaaatggag
     3061 gatttaggat ctaagctaag agaattaaga gtgaaaaaag ggtatactca agaatatgtg
     3121 gcaagaaaat ttaacgttac acgtcaaact atttcaaagt gggaaaataa caaaacaaca
     3181 ccagatatag agacactaaa aaaaatttgt gaattttata gtactaacat aaattctatt
     3241 ttaaatgaag ataattcaaa aaataaaaaa aataatttac tggtattgac agctattatt
     3301 tttaatatat ttgttactat attaggttta tttgaattga ttcaaatgaa tttttttagc
     3361 tttacacatg gaatatttac ttggtttgct ctatctggat taatagtaat agatatctta
     3421 aattattttt atatttacta ttcttattta aaaaatgaaa aattttttta tacaatgttt
     3481 ttccccctag ttattttgat tattttatta agctaatatg aagtagttag ttatttaatt
     3541 gcatagttga cataatgtac actatgccaa gtagcccctc taccaatgga tagagacgct
     3601 cactttgtga tttttaattg gtctagctaa aagcaacttt ctaaagcttg atttttcgcg
     3661 gttatctata tctaattctt tgtgcatttt tatctgtcat ttttttaaga cttgttattt
     3721 tttgtaaaat atggtaaatt tttggtagcg cttcataagc gcgtgctttt tcaagaataa
     3781 acactcatta ttttttaact ggctgctcgg aagagcagtc ttttttatta tttatccgtc
     3841 tcttttacaa acaattcttc gttaggactg ttgtaataat ttggattcaa tttcaattca
     3901 ccatgtttat tccctttatt gattactgac aataacatcg gactgaagcc tagaattcct
     3961 agtaaaatag aaccgaatag aagatttttt cttgtcatga atgttccctc cagttatttc
     4021 caaagttgcc accatttttg cttcttcttt tttgtgtgtt tttctatcga ttctgtttct
     4081 tcagaaagaa tagtagttga ttcatcttct ggagtttctt ccgtataagc taattgtaac
     4141 tgttcttgca gtttttcgat ctgttgattt gcctgtaggg tgagctgttg ttgctggtcg
     4201 agtaattttt gcatattaga attattttgc tctttgaggg aaagttgttc tcgtaaaaat
     4261 tcattttctt cacgcaactt atctagcaac tcgtttacaa tcacgttttt gcttgatata
     4321 taaacgtttt tatcgttttc ttgttcatac tcatgcaacc tatatagtaa cctttggtta
     4381 cctaagttgc tagcaagttg caatactcca gaacgtaata caaccttata accacgctca
     4441 tttttagcta ataaggacgg gtctaattta tcaacatgtt tagagattgc ttgcctactt
     4501 atttcaaata attcagataa ttcctttatt gtaaaggttt ctttgggttg catgataatt
     4561 gcctcctaaa ttgttaggta ttatttctct aaccaattat ctaaggtaat tgaaggtata
     4621 ttttcttcaa aataaatttg cagagatcct atcttatttc ctttgtaggc tttaattttt
     4681 ttgatttcaa aagttttaaa aatagcaggg tgagttgaat taacgtttaa aaattctttt
     4741 ttcgcttggg caaaaatttg tttatcaata tctctcatgc gataactttc tggtattgct
     4801 aatagagctc taaaatcttc tatttttaca aaccagtgac cttttttaga tcttctgtct
     4861 ttatgagcca taagttggcg atataactct ttaacgtagc tactctttaa gtttgtcata
     4921 ttttctaatt cgaaacgagt aaaattagtt ccgattgaat taagtatata ttcaaattct
     4981 tcagaaacct ttattaataa tgtttcattc actttatcga ttttaaattt ttgaaacaga
     5041 tttaattgtt catatgtttt ttcgtcttca taaataaaag acaaagtaat gatttttttt
     5101 gacattttta ttaaatcatt agcaaaacgg tcgccacgtg catttatatt gtaatcaatt
     5161 aattctttta tttgtttaaa tgtaaaaatt tcttcttttg taccttttct tttcatttta
     5221 gaacatattc cccaaaataa atccatatct ataggggtaa attttcttaa agggacagaa
     5281 tttaatccat tattatatcg aactatatcg ttactcaata aaaatcacct caataaatta
     5341 tgttatcata gacataagag gatgtaaaac acaatttatc ccccatagtg tgacaattta
     5401 tcccccatag tgtgacaatt tatcccccat agtgtgacaa tttatccccc atagtgtgac
     5461 aatttatccc ccactaccta tgtcaatact tactctccca taaggttgct aatccctaac
     5521 agttattatt aacagtatat ctaacagtat atctaacagt atatcttaag agcacattga
     5581 atttcccgcc taaaaaactc ctactttttc ctccaaaaag gttcgcgaat ttttctaaac
     5641 tggaaaactt gctccctacg gtcgctaaaa aggtgtgatc gctatgctct catattatac
     5701 tgcgcagtcg cctccggctc ctttgctaaa accgttgttc aaagaggagg tgtgagactc
     5761 tgaaagcttg tctaagccgt tctaagacaa ttcttgatta atttccctta atctagtcga
     5821 gaagcgcctt acaggcggtc agggcaagcc ctaacaaccc taaaacaagt gatacttaaa
     5881 aacaccacac aaatttaatt ttttaagata taataacttg tcgcctatcc gacagtcttg
     5941 cgactggaag gaggtgtatc taatgtttga actgttcatt tgcccccttc ttgtaggagt
     6001 ttgtgtctct ttattcagtt attggttgaa taaccgaaac aagaagtaaa atcaataaag
     6061 gcgacatcaa cctgaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     6121 aatgtttgat catttgcaca ttcagtttaa catgatttta aatcaaattc aattttgttg
     6181 ttgctgatct aaccattcct tgacactttt tagatagttt aaggcggttt ctttttttgt
     6241 cggattttgc atgttagttg cttgttgaag taagatttca tagcgttcat atgtgacaga
     6301 ttgttgtttt tctttttttt cttcttcact ggcaaaccaa gggtttgtgt ctccaaatag
     6361 atcactacag gcttcttcta tctttgtatc ttttacatat tgcacgtctt ttttggttcg
     6421 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     6481 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     6541 ttcctcataa caagtggtaa actcttccat tttttgtgct aatgcttgct cttttttctt
     6601 tttatcacgc gctgattcat acttttgagt gacatttcct aataaaatac ttctagaaac
     6661 gcgcttattt ccctctaaaa cgccgaataa gccgcttgaa acgattatca aggaaaatat
     6721 acagccaaga aagaaaaatc gcttaaatcg aattttagag ccttttaatg aatatcttgt
     6781 ttgaaaaatt ttatagatga gccaaatact tcctgaaata ctaacaaaaa tgagtagata
     6841 gggtaaataa aatgccattt tagcctcccc atactaacca aaatgcgaac agtgtcagga
     6901 ctaagcaaca taagggggca agaaaatcca atgatcgttg aataagcacc tttttctgtt
     6961 ccttatttcg ctggtctaat agctcttgat ggagtttttt aacttgtaaa tcaatcgtag
     7021 tttgtatcct ttcttgcgta taaggaacga gaaaagcacg ttgtttcttt atttcgtttt
     7081 ctatgtcttt taacgtttta gagcgataat tcttgttggt ggtctgttcc tctttcaacg
     7141 tgttgttcat ggtttcttcg agccggtcta atttccggtt gttcaggcgt aagagttcgt
     7201 tgttctcttc gtacttcttc tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg
     7261 ttggttcgct gttggttata ggttcgttct cgtctgtcca gctcatgtaa aatcgtctcc
     7321 ttttcgtagt cctttcctag tctgttggat cgtacttttt tctttttgtg gatcaattcg
     7381 taagtgaaat ttttcccgcg attgaagcaa tgaatcccca atgttttgag ggaattacaa
     7441 aattggtcat aggttctggt ttttttgttt ttcattgttt gatcgatcgc aatccgaatt
     7501 tcgtctttcc aaacaggttc atttttttct tttaatttta tttcagacat ggttcgtttt
     7561 tctggtttct ttcctcgctc agtgattggt aagttatgtg caagacaaat ctcgtcattt
     7621 aggtcataac atttgtttag aaatgcttga ccatgagctt gaaatttttt tccagtctcc
     7681 agattaacgg agttaatgac aatgtgatta tgaatatggt ctttgtctgt atgtgtgtaa
     7741 atagctattt ggtgatcggg ggctattttt tttgcatact ctgcccctag ttgattagct
     7801 ttttcagcgg taatttctcc tggtcgaaaa gattgaatca ataagtgtgc ctgtacacgg
     7861 tcgtcttttc catacagcat gcgaatttgt ttcatttcac ttttggcata atcgacgtca
     7921 cagtttattc ctgttttaat ggttgctcgt ggttctgcgt aattaattgc acgactacaa
     7981 gaagtggttc ggcttacctt aattgttgcc atatcttttg tagctccttt tgcatgattt
     8041 ttaggttttc ggataaacgt tgagcgtcac cagaagcaac gtcatgttgg tgtgtattgc
     8101 accacttagc aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct
     8161 gggcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca
     8221 cgaaagccgg cacactcatt tgcaaagctt cagccgattg cttcagcttt gcaaattcgg
     8281 attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaag
     8341 aattgctagc caaattttga ttttgttcgc tcataagtcc caccctttca gtacgtccta
     8401 cggacgtatc gttcgcaata ccattgtacc taaaaaggta caatgagtaa agctcactaa
     8461 aagggtttgg caagctctat atttgctagc caaataccta cgtatttagt gcaaataacc
     8521 gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc aaaagccacg ctccacaata
     8581 caaaaaaagc tagcaaatgc tagccacttt ttgtaaagtt tcgcttattt caccgaagat
     8641 gaaattgttg ctttgcaaca aaaaacataa atgctttttt gtgtaaacta ctaactattt
     8701 agctaaaact gatagtttta ttaaacattc agcaatattt ttagccaatt ttttatcaga
     8761 ctttcggcga gtcattatga taatatgtcc actttttgtt acttctacca aaaatctagc
     8821 aataactctc ccattcttca gttgatttag tttcataagt cttttgtaat tgacggttta
     8881 atttttttgg caattgcaaa gaataaataa gtctatcata aaatttttca tactcttttt
     8941 tcaataaaat cacttgggtg cctccttttt attaagtgat tatttaaatt ttgggatagg
     9001 agagatggaa tataaaataa atacccgtgg tgctagtttt aaatcagcta gcatcacggg
     9061 taaaaattat aattctataa tattagttcc atagtttaga acatttaagt catgtgttac
     9121 tagtacaacg gctttttttt ctttttctgt taaatttttc agaaagttca ttataaaagt
     9181 tgagttttct ttatctaaat ttcctgtagg ttcatctgca aataatatat caaatggttt
     9241 taacatattt ctaattaatg ctattctttg ttgttctcca ccgcttaaga gaaagatttt
     9301 ttcttttaag atatct
//
