LOCUS       NZ_CP064347            10814 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Enterococcus faecium strain VRE3363 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP064347
VERSION     NZ_CP064347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15566645
            Assembly: GCF_015476295.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Genomic surveillance of antimicrobial resistant bacterial
            colonisation and infection in intensive care patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Wyres,K.L., Hawkey,J., Mirceta,M., Judd,L.M., Wick,R., Gorrie,C.L.,
            Pratt,N.F., Garlick,J., Watson,K., Pilcher,D., McGloughlin,S.,
            Abbott,I.J., Macesic,N., Spelman,D.W., Jenney,A.W.J. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: Enterococcus faecium VRE3363
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 128.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 05:28:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 66 of 131
            Pseudo Genes (incomplete)         :: 67 of 131
            Pseudo Genes (internal stop)      :: 26 of 131
            Pseudo Genes (multiple problems)  :: 22 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064347.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE3363"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            join(9474..10814,1..153)
                     /locus_tag="IU206_RS15275"
                     /old_locus_tag="IU206_15275"
     CDS             join(9474..10814,1..153)
                     /locus_tag="IU206_RS15275"
                     /old_locus_tag="IU206_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            288..584
                     /locus_tag="IU206_RS15280"
                     /old_locus_tag="IU206_15280"
     CDS             288..584
                     /locus_tag="IU206_RS15280"
                     /old_locus_tag="IU206_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(608..1567)
                     /locus_tag="IU206_RS15285"
                     /old_locus_tag="IU206_15285"
     CDS             complement(608..1567)
                     /locus_tag="IU206_RS15285"
                     /old_locus_tag="IU206_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222573.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            1750..>2355
                     /locus_tag="IU206_RS15290"
                     /old_locus_tag="IU206_15290"
                     /pseudo=""
     CDS             1750..>2355
                     /locus_tag="IU206_RS15290"
                     /old_locus_tag="IU206_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2430..3110
                     /locus_tag="IU206_RS15295"
                     /old_locus_tag="IU206_15295"
     CDS             2430..3110
                     /locus_tag="IU206_RS15295"
                     /old_locus_tag="IU206_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            3308..3913
                     /locus_tag="IU206_RS15300"
                     /old_locus_tag="IU206_15300"
     CDS             3308..3913
                     /locus_tag="IU206_RS15300"
                     /old_locus_tag="IU206_15300"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            3929..4501
                     /locus_tag="IU206_RS15305"
                     /old_locus_tag="IU206_15305"
     CDS             3929..4501
                     /locus_tag="IU206_RS15305"
                     /old_locus_tag="IU206_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(4974..5546)
                     /locus_tag="IU206_RS15310"
                     /old_locus_tag="IU206_15310"
     CDS             complement(4974..5546)
                     /locus_tag="IU206_RS15310"
                     /old_locus_tag="IU206_15310"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_073120187.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGF
                     YNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="IS3 family transposase"
     gene            complement(5647..6809)
                     /locus_tag="IU206_RS15315"
                     /old_locus_tag="IU206_15315"
     CDS             complement(join(5647..6533,6533..6809))
                     /locus_tag="IU206_RS15315"
                     /old_locus_tag="IU206_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            complement(7215..7469)
                     /locus_tag="IU206_RS15325"
                     /old_locus_tag="IU206_15325"
     CDS             complement(7215..7469)
                     /locus_tag="IU206_RS15325"
                     /old_locus_tag="IU206_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(7662..7937)
                     /gene="prgO"
                     /locus_tag="IU206_RS15330"
                     /old_locus_tag="IU206_15330"
     CDS             complement(7662..7937)
                     /gene="prgO"
                     /locus_tag="IU206_RS15330"
                     /old_locus_tag="IU206_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(7909..8862)
                     /locus_tag="IU206_RS15335"
                     /old_locus_tag="IU206_15335"
     CDS             complement(7909..8862)
                     /locus_tag="IU206_RS15335"
                     /old_locus_tag="IU206_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     Typing          9475..10814
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.851"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caactaagta aagaaaagag actagattat tcaacaagga tcacagaggt atttggactc
       61 aacgagagct tagtagaaga gacatttaga caattatcaa cccccttgaa ccctcttaaa
      121 gaagtgaatc tttttgaagt agatacaggt tgaaaaataa aaggtgcaaa ttgccttata
      181 caatatatct atgatacttc tgtaggtgtt gctccctttg cctagctacg tgtaggtggt
      241 atacttataa ctgcgtatgc agtcttatgt atcatggagg aagttgtatg tcgttaaaaa
      301 attatgtcta ttcacatcct gttaatgtgt ttatcattgg tcgtttggga ttatctgtgg
      361 aagtgttctg tgagctttat ggttttaagc aggggacttt gtcttcttgg gttaccaggg
      421 aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg gttgtcctta gcttctgggg
      481 agactatgga tcaggtatat gattgtttgt gtgtgttaga gcaggagtat attgagtata
      541 aaatagctaa cgagttaaga aaaaggaaga agtatatcca gtagtagttt ttatttactc
      601 ttaattgtca aattaagtta gacaaatcat caatacttac gtaactcaaa aatacttcca
      661 aaggtgttcg ataattcagt gattttctag gaatattatt tcgtttcgat gcgacagatt
      721 gaataaaaga ttcatctact gaattgaaat ccatagattt caataaacca tctctacgta
      781 acaatccgtt agaattctcg tttaggcctc tttgagacgg cgttcctgga tcagcgaaat
      841 aaatggcaat atcattggca ttactgatct gtttccaatt tgaaaattcc tttccacaat
      901 caaaagtgat ggatttgaat agatttttcg gtacagattc aaaccaatga tttaatttgt
      961 tttcaatatc aatcgcttgt ctaccacacg gtttcaatgt gatgataact tttgataatc
     1021 gctcaactaa ggtaattaca gcacttttat gtttcaaacc tacgatagtg tcaccttcaa
     1081 ggtgaccaaa ctcatttgag aattggctat aatctttttc acgttcatga atagagcgac
     1141 ggaaagcttg ttttccacgt ctttcttgat gtccatttgg tttacgcttg cctttcatcg
     1201 gtaagtcaga aggattaaat agcccctttt tgaacatacg ataaatggta cgagcagaac
     1261 aacgaatagg aaacgctgca cgaccaacaa tcacatcggg tgtccatcct tgaacaacct
     1321 ttctttgaat atattctgat tgttcctcag gtaaaacaag cgggcgtcta ccacagtttg
     1381 atttgttttt cttatactgt tgatagtact ctaaggctga ttttccttgc ttgaagaaca
     1441 ggtatacttt atggatcgtt tgtcttgaac gattcaagca atgcgcagtt ttcgcaacag
     1501 attgatttat tttgaaataa gactctatca ttacaagttc atccgttgta agatgggtat
     1561 aggtcatttg tactcactcc ttataattct ttggacggaa ctattttgag tgtagcacaa
     1621 atgacttttt tagttgtcta gcttaatttt acaatcggcg tacttaaaaa attggttcat
     1681 tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt aaaggaaaga atttagggaa
     1741 gaaatcgaaa tgaaatcgga cgactggatt gaacacacct ggcttcctct gaaaagaaat
     1801 agcaaacgaa agaaattttg ggtattgcaa ttatggccat taggaaaact ttgtagttac
     1861 ggggcacgac ttattcttga tttaaccgtt tactcgattg gagtatttgg aacggcttat
     1921 tacttgataa atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg
     1981 ttgatttttg tcatgctatt gtctattttt actcaattag tcatgcaatt taccaggcaa
     2041 aaattaacag caggaaaact tttcaaaagc ctattcgatt taattgttgt aaatgcaatt
     2101 ctagcgactt taataaaaca aattgaacaa tctggaagaa caacaaaagg agtgaaggat
     2161 tttttattga cgtttattca ctttgtaaat ggacacatgt atttgtttga agtgggtatt
     2221 attctctttt ttgtcgggta tgtatccacg aaacttatta aacgttcaat tgaaaaagaa
     2281 attgaaattg acctgattcc gacacttctt ggttatgagt acaatgttga ttttggaaga
     2341 tatgacgaat tgatcggttc tgttgcaaag ttttaaatct actatcaaat aaggtagaat
     2401 aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag caatttcagc
     2461 aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc tatcgtgaag
     2521 ttcaagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt tatcgttggg
     2581 tgcaagaata tggcaaacta ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt
     2641 attcatggaa aatggatgaa acgtacatca aaattaaagg aaaatggcat tatttgtatc
     2701 gagccatcga tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac
     2761 aagcagccta tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag
     2821 tcacagataa agccccctct attacaagtg cctttaagaa actaaaagaa tacggctttt
     2881 atcaagggac agaacatcgt accattaaat acctgaataa tttgattgaa caagaccatc
     2941 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct accacgatta
     3001 aaggcatgga agccattcga ggattatata agaaaacccg aaaagaaggc actctcttcg
     3061 ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata
     3121 ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccaactag actgtgcaaa
     3181 atagagacac ttgaaaaatc tatcgggggt cagaaactta atagtttttg acacataaac
     3241 aaaatcgcga tataatgggg ttgagagtgt ttcataaact tatgtcataa agttaaggag
     3301 atttcgcatg attttagaga ataaattggg attaaccaat caagttgagt tggctaaggt
     3361 tgaagaaaaa ctcagtaaac aaaaagcgaa agaattgtac gattgtggaa aaataaatga
     3421 aatcgaagtt ggtacattta aaggtttatc tgaaattcat gaatttctat tttcggatat
     3481 ttacgatttt gccggaaaaa tccgatcggt caatattgca aaaggaaatt ttcgatttgc
     3541 accagttatg tatttagaac attcgttaca gcatattgat cagatgccac aaacttcttt
     3601 tgaagagatt atcaagaaat atgtggagat gaatatcgct cacccatttc gagaaggaaa
     3661 tggccgaagt actagaattt ggctggatct cattttaaag gaagaattgc aaaaagttgt
     3721 agattggaac ttaatcgata aagcagacta tctttctgca atggagcgaa gtcccataaa
     3781 cgatttggaa atacgctatc taatttccaa tgctctgact gataaaattg acgatcgcga
     3841 gttatatatg aaaggaatcg atgtcagcta tttctatgaa gggtattctg agtatacgat
     3901 agatgatttg taaaagtagg tggaccaaat gacgaaatat ggatatgcac gtgtctcaac
     3961 cgcttctcaa agtacaaaag aacaaattca acagttgctt gagaacggcg tagagaagaa
     4021 gaatattttt tcagaaaaat ttacgggtac aaaacgggat cggccaaaat tcaatcagct
     4081 gttggctggt ctgaagcctg gagatgaaat agttgttacg aagcttgatc gatttgctcg
     4141 taacacgaga gaagcattag agatcattga acctttactc gatgataacg taacgatcaa
     4201 ggttttgaat ctaggaacga ttgaaaatac gtccatgggg cgaatggtga cacggacgtt
     4261 gttgagtgta gcagaaatgg aacgtgatat gattgtggaa cgtacccaag aaggaaaaat
     4321 gtttgcaaga aagaataatc ctaatttcag agaaggacgt ccgaaagcaa caatcacacc
     4381 gaagaagagg cacgcatatg aactgcttac ttcaggtaaa tcgtataagg aagttgaagc
     4441 tataaccggt ttcagtcgca gtacgctttt tagaataaag aagaaaattg aagaaagtta
     4501 aaaaagaagt ctcagcgaac cagacaggta aatacttaat gcgttctaga tatgagtcta
     4561 ttagcatgtt gtgaaatata atattttttt atttacttga tttctgctca aaaaatgtgt
     4621 atggtaactg taaaaggtaa agtaattaaa ggtatgctag ccacgcttac tatattagga
     4681 taccatcata tatttataat gaacgatttg ttcgtctacc aaacaacaca tatcgttcag
     4741 cgtaagtcac gtttaggaac gtggcttttt tagtgttatt atcgataaat aatctattga
     4801 ctctattgat tttatctcca tatccctacg gtgttgattg tctttagatc tttcatttaa
     4861 gaaccaaata actatcataa aaatcagcac tgctgctaat ataataaaca gcattcctat
     4921 tgtgatatta ctggatttga gtcaatatct tggacacatt ttagtagaga cggttaagcc
     4981 attttttctt ttgctaattc ttcaaattcc tgtggggtta gatagtgaat cgagctatgg
     5041 attcgatttc ggttataaaa tccttcaatg tagctaaata gcgctcgatt tgcttcttca
     5101 aaatctgagt agctagtcgt gtagacttct tcttttttca atgaggcatg gaaagattcg
     5161 attccagcat tatcataagg tgttcctttt ctactataag aatgccttat tttgttggtt
     5221 ttaagccatt gttccacttc tcttgctgtg tattggctgc ccaagtcagt gtgtagaatc
     5281 attccttctg gaatgtgata atgtattttt gctttattca atgtttgaat gacacaatcc
     5341 acagtcattc gttttgaaaa tgtataacta ataattcttt tcgtgtgtaa atccatgatt
     5401 gaagataagt aacaccaacc atttttctta gtaggaatgt acgtaatgtc agctgcccat
     5461 ttctcacaga tcgtttcagt agaaaagtcc tgatttaaga gattctcttt tgaaatgatg
     5521 gaatggcaac agtttttttg acaaatttta taaggtgcag aacttctttc cgtatgctat
     5581 tccgattgtc cttgacaatg agcctcctcg gatgtgacga tcttcggcag gagctaacag
     5641 ttaaagttag actgcctcgc tagcgttagc acatccgagc tcattatcaa ggattcgctg
     5701 cgccaatctc tggttacggt aaccaaccgg tgtctcgtag ccaagtgtac cgtgcaaccg
     5761 aaggtggttc caccaattga catagtcaaa taactccaaa tccaattgtt gtaaggtttc
     5821 aaatgtgtat tgatagacaa attctacttt caacgactta taagttgatt cagctacggc
     5881 attatcaaaa ggacagcctt tatgactcaa tgatcgattg atgtcaaaag ttgttaataa
     5941 ttcatcaata gcttggttat caaactcttt tccacgatca gtatgaaaaa tctcaacctc
     6001 tgtcagaggt tgtttgatac ggctaaatgc tttttttact agaacggcat ctttatgttc
     6061 tccacaagaa tagccgagaa tttctcgatt gaacagatcc aaaatgaaac agacataatt
     6121 ccattttttc ccgactcgta cataagtcaa gtctgttacg atcgcttcta atgggttgtc
     6181 tcttaagaat ttacgattca atacgtttgt cgttttggct tcattgcaag tagaatgatg
     6241 tactttaaaa taagcaacag tatagctcga ttttaatcct ctatttttca tgattctact
     6301 aatttttcgt cggctgatct gaatgcctcg ttttgataag gcttttttta tttttcttga
     6361 gccgtaggcc tttcggctgc ggataaattc ttcagcgact acttcttcaa gttctgattc
     6421 gtctttcttt ggttttgatt gataataata ggactgacgt gatagaccta atattctgca
     6481 catcgctgat atagggtaaa gatgcttatt cgcatcgatt acttgtctct tcgtccgaat
     6541 atcagcgctg cttgctttaa aatatcattt tccatttcta attgctggtt tcttttacgt
     6601 agttctaaca attctttttg ttcaggcgta agattatctt tttctttgaa tgaaccactc
     6661 gttttaaatt gctttaccca tttgtcaaat gctgaagccg ttagttcata ttctcgaatg
     6721 atttctacac gtggctttcc agctaagtaa agattgacga tttgttgctt gaattcttgt
     6781 gaaaaagttc ttcgtgttct cttagacata aaaattcctc ctggtatgtt ttcttctagt
     6841 ctacacacct taatttttct gtctagttaa ttgtagccta tccatgatcg gtttattaga
     6901 cctttgaggt ctatattttt taactacaat cgaccttaaa tttaattgtt tcattagctt
     6961 ttggacatgt ttaagagata ctgaaatccc ttcttttaat aaaacttgat ggatctttgt
     7021 ggcaccatag cgttgtttat tcgtaaaaaa aattgttgaa atcttctttt tcaacttatt
     7081 atttctgatt tctgtagctg tcaacggttt attcttataa aaataataga cacttctagg
     7141 gatttctaat aaccgacaca acaaagaaac agtgtaatcc ttacaccatt tttcaataaa
     7201 ttgaacaagc gaatttaggt tttccgagag aatatggctg cggctttttt taagatttca
     7261 agttcctcat ttaaacgagc attttctttt ctcagttcag ctacttcttt tccagtcagt
     7321 cctgtggact gatcaggtaa atataaattc ttccatttgt aaatcgttgc ttcagataag
     7381 ccatattctt tcgctaaccc tcgaacagaa cgtccagttt gattcagttc aacaatcatt
     7441 tgtttaaaat tttcttcata tcgggtcata aaaattcatc ctctcgtaga taagtttaat
     7501 ttattcttgt ctctacgaaa agctgtctaa gatttgatac taacaccaaa tgttgtatac
     7561 ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt
     7621 atactaaata tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta
     7681 ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg
     7741 actatttcat acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg
     7801 ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg
     7861 acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc
     7921 gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat
     7981 tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta
     8041 ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc
     8101 tacataatca tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt
     8161 aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc
     8221 gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg
     8281 agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc
     8341 ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa
     8401 tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt
     8461 tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt
     8521 ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg
     8581 tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga
     8641 tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg
     8701 gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa
     8761 atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc
     8821 tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct
     8881 atactaaata tatttagtat acttcatata cttaatatat tcgatatatc taatatactc
     8941 caaattgata agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct
     9001 atattcagta tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac
     9061 aatttagagt gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta
     9121 caaagtagca aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg
     9181 aaataattga ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca
     9241 cgctcgtctc aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata
     9301 ccttttccga ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaacgttt
     9361 atttagtgta cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg
     9421 agattaaagc taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga
     9481 gcgaaattta ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca
     9541 ccatgaaacc tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta
     9601 gcaagcaatt aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag
     9661 agaacgaaaa gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg
     9721 atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat
     9781 ttgtagttga tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag
     9841 atcaagtggg ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt
     9901 ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag
     9961 caaaaaaaat ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc
    10021 taggatgtaa ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag
    10081 aaatgaacta tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag
    10141 atcaaaaaac aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa
    10201 ttcgtcaagt agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg
    10261 gagaaggacg tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt
    10321 caggttatgc attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg
    10381 aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc
    10441 aagcagcaag cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa
    10501 aaaataatga tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc
    10561 aaagaaaata tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa
    10621 aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa
    10681 cgatgcccaa gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct
    10741 tctacaaggt aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt
    10801 tcgcgagggt tatt
//
