LOCUS       NZ_CP064262             5596 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JX-CR-hvKP-10 plasmid pJX10-4, complete
            sequence.
ACCESSION   NZ_CP064262
VERSION     NZ_CP064262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16561435
            Assembly: GCF_015355875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Prevalence of the NTEKPC-I on IncFrepB plasmids among Hypervirulent
            Klebsiella pneumoniae isolates in Jiangxi Province, South China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Yong wai zheng jie No. 17, Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 261x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 06:54:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,062
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,751
            CDSs (with protein)               :: 5,751
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 67 of 187
            Pseudo Genes (incomplete)         :: 129 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 33 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JX-CR-hvKP-10"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="pJX10-4"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018/2019"
     gene            join(5441..5596,1..171)
                     /locus_tag="IS497_RS30055"
     CDS             join(5441..5596,1..171)
                     /locus_tag="IS497_RS30055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            197..742
                     /locus_tag="IS497_RS30060"
     CDS             197..742
                     /locus_tag="IS497_RS30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            808..1158
                     /locus_tag="IS497_RS30065"
     CDS             808..1158
                     /locus_tag="IS497_RS30065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1228..1494)
                     /locus_tag="IS497_RS30070"
     CDS             complement(1228..1494)
                     /locus_tag="IS497_RS30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1478..1702)
                     /locus_tag="IS497_RS30075"
     CDS             complement(1478..1702)
                     /locus_tag="IS497_RS30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2369..2581)
                     /locus_tag="IS497_RS30080"
     CDS             complement(2369..2581)
                     /locus_tag="IS497_RS30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2588..3097)
                     /locus_tag="IS497_RS30085"
     CDS             complement(2588..3097)
                     /locus_tag="IS497_RS30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3132..3836)
                     /locus_tag="IS497_RS30625"
                     /pseudo=""
     CDS             complement(<3132..3836)
                     /locus_tag="IS497_RS30625"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3775..4119)
                     /locus_tag="IS497_RS30095"
     CDS             complement(3775..4119)
                     /locus_tag="IS497_RS30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4109..4285)
                     /locus_tag="IS497_RS30100"
     CDS             complement(4109..4285)
                     /locus_tag="IS497_RS30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4925..5083
                     /locus_tag="IS497_RS30630"
     CDS             4925..5083
                     /locus_tag="IS497_RS30630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          complement(4372..4448)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4637..5034
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2254..3785)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagaaacc ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct
       61 tcaccattaa aagaatacca agaaaaaatg gatgagatag attcaattat taatgacatg
      121 atggttctaa aggaatattt cagagctaag ttttacgacg aaataaaata ggcacgctat
      181 acggaggcgg agaattatgt tttttaaacg gtatttaacg gagaaggaaa tatacaaggc
      241 agcactccca attgttaggc ttcaagggag agttaacact tcattacgtt ttttatccaa
      301 atactcagat gtaaacaaaa atggattaat tgcattgcat cttaatttat tacattttat
      361 ctcatacaac atacacggtg tagagtctct gaaaatcact caagcaggca taaacaaagc
      421 tgtacaacaa agccctcacg cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa
      481 aggacctgag tacatcgaag gattaattag aaacccatgg gggaagaaaa atgataatcc
      541 actaatattt ataatgagca ttgaagaaag tataaaagaa acccttaact caggttatat
      601 gttatataga gtagctccca taactgaggg tgaagcagct gctttcatgg atgttcttaa
      661 acaattatta ttatttgagg ataattttcg cctttttggt agagacttgg tttataaaat
      721 agaaaaatta aaaatcagat aacaaagtat ggtgaacgaa aaatgaaaga gaggatagtg
      781 aaatgtgtaa taccaagatt ctcaactatg tattggttaa ttgcgttcct agcatttata
      841 ttccccggtc gatcttttgc tgacggttac accgcttact accgatgtaa tggtactcag
      901 gaagcatctc ttactgttaa agataacaaa ctaagtatgg ttgtaggtct ttctaagttt
      961 aataggcaac tggatactta tagattcaaa gaaaagggaa tcgaagtagt aatgcagaga
     1021 gctgcttctg atgataactc tgaggccata atgtctttta ttatgcctga cgttaacaat
     1081 atgaccttat tagttaaaaa taacggctac attatcgtta agacaacctg ctggttaact
     1141 aaaaccgaca aacagtagaa cacgtagtta gtcgagtatt taccataata cctgttgtcg
     1201 gcatcaaaaa agcccgcata gcgggcttca gtcgtaaact tcgcggcgat gtccaatcgt
     1261 ggcggcaagg ataacaagct cgtcatctcg gatctcacac agaacgcgat aatctcccac
     1321 ccgatagcgc cagaactcgc ccagctcacc tttgagcggc ttccctgact ggcgaggatc
     1381 ggcagcaact gcaatgcgta aactcataaa atccacaatc cgtcgtgcgt tctgtttgtc
     1441 catcttgcgt aacgatttga gcgcccgatc ggaatagtta atcgtccaga ccaagtgact
     1501 tcctcacgtc ttcagacgaa tgcactgctt catcaccacg gcgaacgcgt gcagcagttt
     1561 ctgctgaaag ataataatcc tccaggtctt ccagatgcgc cagtattgct tcccgcgcat
     1621 aaaacgtctt tgttctgccg gtttgcttcg ccagcgagtc cagacgggac tcaatctcat
     1681 cagataaccg gatagcaagc atataaacct cccgaacacc atttgctata catgtatatc
     1741 aacagtacca caatcaacct aaaagaacaa aaaataatct tgttagcccc tcttgataat
     1801 ccgtaacgcg gtcaatatat ctagcttgag ggctttcctc ttaactattg aaaggataac
     1861 gattatgggt cttaagccgg gtccgaaacg aattgccgat tcaacagggg aacctgataa
     1921 acgccagcgt gacaataaaa aaacgccggg aaatactgac aaattgaaac ccagcaaatc
     1981 ttcaaaaaaa taagtcagta gttaaaagcc ctccggggct tttttgtatc tgccgttcac
     2041 gctgattaaa cataacaccc gttgcccgca ccgccccgga cgggttcgcc gcgaacgggg
     2101 tggcagtcag gtaaaaccgg caacaaacca cccgataaac cgccccgttc gcgacagata
     2161 aattttacct ggctgcatag ccatgcagcc aggtgaatga catccggttt cagcgtaaat
     2221 gcccgatgag aaccctgttt tcctgcattt cagcccagcg acggaccgtc atagcttcgc
     2281 tgccgggaac gcgcttccgt cagttccttt tcgtgctgcc gttgctcgtg cgcccgttct
     2341 gcggccacgg ccatctcatg acgctgcact accggctcaa acgctgcagt tgcccgctca
     2401 agcgttccac ctgctgactc aagcgcgtca cgcgctcgct cagcgccgcg ttctcccgtt
     2461 gcataagccc tgacattttc cgccattccc cgaaggcgct ctcccactcg tccagccttt
     2521 tcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgtttttcc agctcggtca
     2581 tcggcactta ctccttcacc agtctcagcg cgttctgctt cgaccgcccg ttcccgaccg
     2641 tccagctctg cgtcctgtct acggccgtcc ccttcggcag caccagaaaa cgtccgttgc
     2701 tgtcttccag gtacgtcacg ccccaggtct tcgcgttcag cttcgacagc gctgcgttct
     2761 gccggtcgat ttcggccaga tttgacgcta tcaagctccc ctgataccac aacaccccgc
     2821 tcaggctggc gaacagcagt cctgacacca tcgtgatggt cagccatgtc cgcccgacca
     2881 tacgcatcac gctcaggcgg ttcgactgca tggccgcgct cataccctgc tcgtgggcgc
     2941 ggatggcggc gctgattctc ttcccgcttt cgctcagttc cgctctgaca gacttctcgt
     3001 gctcgctgaa tgcggctttc agcatttcgc cggtactctg ctgctgcgct ttcgatttct
     3061 gctctaagtc cttcgccagc gttaaaagac tgttcataga tggctccctt aagtcggatg
     3121 tttcgccctc cgtccgggtc ggcaatgctg atgctgctct ttgtggtgcg cacgacctca
     3181 aaacctgcgc tttccagcgc ctcagtgacg tcctgacgcg ttttaagctc ccctgacgag
     3241 gcgagggcca gtaagccccg cgtaatagcc tctgcggctt cctgcttcgt tttgggtaac
     3301 gaagatggcg tcaccagcgc gcgccggttc tccggtgcgt tcgggtcgtg cagccccagt
     3361 cgcccgttca ccacggtctg ccacgcatcg atgcgcggac ggtctgcccg gtcgtagtac
     3421 ggctggagac gcctgccggt cagcagttcc gtgttcggta ccagaaaatt cagttccagc
     3481 cgccccttgt cccggtgttc aacccacagc acgctgtact ggtctttatc gagtccgggc
     3541 atcagaaccc gctcgaagct cgccatcagc ttttcacgct ggccgggcgg taaatcctgt
     3601 tcagcaaaag acaggacgcc ggaggtgtac tttttggcgt agggcgaggc gtcgataagc
     3661 tcccggacct cctccggctt tccctgcagg acgatggccc cgtcgcgctg gcggtctttc
     3721 cccagcagat aatccaccgg cccggcaccg ccgcctcgcc ctcgcggatg aaacttaacg
     3781 atcatcgtct gccccctttt ccagcacggc atgccgcagc cgctcgagtt cggcatcaat
     3841 ggccatcagc gcggccacca cctgaacccg gtcagcgcct gaccactggc cgccgttaat
     3901 tttccgggcg atctggttga ggttgttccc catcccggca agctggcgca gcaaaaccgg
     3961 atcgatggac ggcagccgca acgaccgcgc cgggcgcgta tcgagacagg tttcccgcat
     4021 ccacaccgcc agttgccttc cattgcagcg ctccagcagc tgctggtact ccccatcggt
     4081 cacccggata cctaaaaaac gggtacgctt aaccgccatc ctctcctccg atatccgcaa
     4141 aatgtgaccg ggctgaacgc tcaagctctg ccatctgctc ccggcggtaa tcttcctcct
     4201 gtgcctgacg catcagattt gccatcactg ccggatgcca ggatcttttt tttacgacga
     4261 gcggcttctt cttcggcttc tgcattatcc ctccttcgca aacgccggac ggcacggaaa
     4321 agttttccgg gtcgctccgg cgttggacaa gcgccagcgc gtcagtggtg ttagcgggtt
     4381 tcggggcgca gccctgaacc agtcacagcg tttacgctgt gtatactggc ttactatgtt
     4441 ggcatgagag cacgattcat gaaagtgcct catgtggcag gtgtgatatg tggctcagga
     4501 gcgtccgcaa taccatgcac ggacagatga aatgacgcct cctcgctcac tcggtagctg
     4561 cgctcctgcc gttcggctgc ggcgggcgtg accgactcac ggataacggg ataaagcaaa
     4621 ggcgctcaaa tcagaaaggg ccggagaaaa ggatgcggcg ttgccgtttt tccataggct
     4681 ccgcctcccc tgacgagcat cacagaaatg gacgctcaag tcagaggagg acgaaacccg
     4741 acaggactta aagatgccgg tgatttctct ccggcggctc cctcgtgcgc tctcctgttc
     4801 cgaccctgcc gtttaccgga tacctgttcc gcctttttcc ctttcgggaa gcgtggcgct
     4861 ttctcatagc tcacgctgct ggtatctcgg ctcggtttag gtcgttcgct ccaagctggg
     4921 ctgtatgcac gaaccccccg ttcagcccga ctgctgcgcc ctttccggta actatcatct
     4981 tgagtccaac ccggacggac acgcaaaaac gccactggca gcagccattg gtaactggat
     5041 atatacgaga gagatattga gaggtcttga agtgggtgcc taactgcggc tacactgaaa
     5101 gaacagtttt ggtatctgcg gtctcgtata cccagttacc cggttaagca gttccccaac
     5161 tgacttaacc ttcgatcaaa ccgcctcccc aggcggtttt ttcgtttaca gtgcaagaga
     5221 ttacgcgcag aaaaaaagga tctcaagaag atcctttaaa tgatcgtcag ttctgttatc
     5281 cccggagctc catccatccc acggaaaaac acccgccact gatgccctca gcgcactgca
     5341 catcatgtac aaaccactcc ctccctcctt ttttattgcg tttcttaatg cgcaatggta
     5401 gctttgtgcg caacaaaaaa cgcaatagca agggggggta atgagagaaa gaacaaatat
     5461 catgctagat ataggattaa agcaatcctt acaacggctt tcgaataaaa ccaaaatgag
     5521 tctaagtgat cttattaacc agagattatc aggttcactt tcagttgaag aacgcattgg
     5581 cgaaaagcct gaatgg
//
