LOCUS       NZ_CP064257             5596 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JX-CR-hvKP-1 plasmid pJX1-5, complete
            sequence.
ACCESSION   NZ_CP064257
VERSION     NZ_CP064257.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16561274
            Assembly: GCF_015356055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Prevalence of the NTEKPC-I on IncFrepB plasmids among Hypervirulent
            Klebsiella pneumoniae isolates in Jiangxi Province, South China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Yong wai zheng jie No. 17, Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 06:54:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,852
            CDSs (total)                      :: 5,731
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 55 of 143
            Pseudo Genes (incomplete)         :: 90 of 143
            Pseudo Genes (internal stop)      :: 15 of 143
            Pseudo Genes (multiple problems)  :: 17 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JX-CR-hvKP-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJX1-5"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018/2019"
     gene            join(5393..5596,1..147)
                     /locus_tag="IS496_RS29015"
     CDS             join(5393..5596,1..147)
                     /locus_tag="IS496_RS29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(217..483)
                     /locus_tag="IS496_RS29020"
     CDS             complement(217..483)
                     /locus_tag="IS496_RS29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(467..691)
                     /locus_tag="IS496_RS29025"
     CDS             complement(467..691)
                     /locus_tag="IS496_RS29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1358..1570)
                     /locus_tag="IS496_RS29030"
     CDS             complement(1358..1570)
                     /locus_tag="IS496_RS29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1577..2086)
                     /locus_tag="IS496_RS29035"
     CDS             complement(1577..2086)
                     /locus_tag="IS496_RS29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2121..2825)
                     /locus_tag="IS496_RS29575"
                     /pseudo=""
     CDS             complement(<2121..2825)
                     /locus_tag="IS496_RS29575"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2764..3108)
                     /locus_tag="IS496_RS29045"
     CDS             complement(2764..3108)
                     /locus_tag="IS496_RS29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3098..3274)
                     /locus_tag="IS496_RS29050"
     CDS             complement(3098..3274)
                     /locus_tag="IS496_RS29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3914..4072
                     /locus_tag="IS496_RS29580"
     CDS             3914..4072
                     /locus_tag="IS496_RS29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4430..4756
                     /locus_tag="IS496_RS29055"
     CDS             4430..4756
                     /locus_tag="IS496_RS29055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4782..5327
                     /locus_tag="IS496_RS29060"
     CDS             4782..5327
                     /locus_tag="IS496_RS29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3361..3437)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3626..4023
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1243..2774)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcagagag ctgcttctga tgataactct gaggccataa tgtcttttat tatgcctgac
       61 gttaacaata tgaccttatt agttaaaaat aacggctaca ttatcgttaa gacaacctgc
      121 tggttaacta aaaccgacaa acagtagaac acgtagttag tcgagtattt accataatac
      181 ctgttgtcgg catcaaaaaa gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg
      241 tccaatcgtg gcggcaagga taacaagctc gtcatctcgg atctcacaca gaacgcgata
      301 atctcccacc cgatagcgcc agaactcgcc cagctcacct ttgagcggct tccctgactg
      361 gcgaggatcg gcagcaactg caatgcgtaa actcataaaa tccacaatcc gtcgtgcgtt
      421 ctgtttgtcc atcttgcgta acgatttgag cgcccgatcg gaatagttaa tcgtccagac
      481 caagtgactt cctcacgtct tcagacgaat gcactgcttc atcaccacgg cgaacgcgtg
      541 cagcagtttc tgctgaaaga taataatcct ccaggtcttc cagatgcgcc agtattgctt
      601 cccgcgcata aaacgtcttt gttctgccgg tttgcttcgc cagcgagtcc agacgggact
      661 caatctcatc agataaccgg atagcaagca tataaacctc ccgaacacca tttgctatac
      721 atgtatatca acagtaccac aatcaaccta aaagaacaaa aaataatctt gttagcccct
      781 cttgataatc cgtaacgcgg tcaatatatc tagcttgagg gctttcctct taactattga
      841 aaggataacg attatgggtc ttaagccggg tccgaaacga attgccgatt caacagggga
      901 acctgataaa cgccagcgtg acaataaaaa aacgccggga aatactgaca aattgaaacc
      961 cagcaaatct tcaaaaaaat aagtcagtag ttaaaagccc tccggggctt ttttgtatct
     1021 gccgttcacg ctgattaaac ataacacccg ttgcccgcac cgccccggac gggttcgccg
     1081 cgaacggggt ggcagtcagg taaaaccggc aacaaaccac ccgataaacc gccccgttcg
     1141 cgacagataa attttacctg gctgcatagc catgcagcca ggtgaatgac atccggtttc
     1201 agcgtaaatg cccgatgaga accctgtttt cctgcatttc agcccagcga cggaccgtca
     1261 tagcttcgct gccgggaacg cgcttccgtc agttcctttt cgtgctgccg ttgctcgtgc
     1321 gcccgttctg cggccacggc catctcatga cgctgcacta ccggctcaaa cgctgcagtt
     1381 gcccgctcaa gcgttccacc tgctgactca agcgcgtcac gcgctcgctc agcgccgcgt
     1441 tctcccgttg cataagccct gacattttcc gccattcccc gaaggcgctc tcccactcgt
     1501 ccagcctttt cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca
     1561 gctcggtcat cggcacttac tccttcacca gtctcagcgc gttctgcttc gaccgcccgt
     1621 tcccgaccgt ccagctctgc gtcctgtcta cggccgtccc cttcggcagc accagaaaac
     1681 gtccgttgct gtcttccagg tacgtcacgc cccaggtctt cgcgttcagc ttcgacagcg
     1741 ctgcgttctg ccggtcgatt tcggccagat ttgacgctat caagctcccc tgataccaca
     1801 acaccccgct caggctggcg aacagcagtc ctgacaccat cgtgatggtc agccatgtcc
     1861 gcccgaccat acgcatcacg ctcaggcggt tcgactgcat ggccgcgctc ataccctgct
     1921 cgtgggcgcg gatggcggcg ctgattctct tcccgctttc gctcagttcc gctctgacag
     1981 acttctcgtg ctcgctgaat gcggctttca gcatttcgcc ggtactctgc tgctgcgctt
     2041 tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctccctta
     2101 agtcggatgt ttcgccctcc gtccgggtcg gcaatgctga tgctgctctt tgtggtgcgc
     2161 acgacctcaa aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttaagctcc
     2221 cctgacgagg cgagggccag taagccccgc gtaatagcct ctgcggcttc ctgcttcgtt
     2281 ttgggtaacg aagatggcgt caccagcgcg cgccggttct ccggtgcgtt cgggtcgtgc
     2341 agccccagtc gcccgttcac cacggtctgc cacgcatcga tgcgcggacg gtctgcccgg
     2401 tcgtagtacg gctggagacg cctgccggtc agcagttccg tgttcggtac cagaaaattc
     2461 agttccagcc gccccttgtc ccggtgttca acccacagca cgctgtactg gtctttatcg
     2521 agtccgggca tcagaacccg ctcgaagctc gccatcagct tttcacgctg gccgggcggt
     2581 aaatcctgtt cagcaaaaga caggacgccg gaggtgtact ttttggcgta gggcgaggcg
     2641 tcgataagct cccggacctc ctccggcttt ccctgcagga cgatggcccc gtcgcgctgg
     2701 cggtctttcc ccagcagata atccaccggc ccggcaccgc cgcctcgccc tcgcggatga
     2761 aacttaacga tcatcgtctg cccccttttc cagcacggca tgccgcagcc gctcgagttc
     2821 ggcatcaatg gccatcagcg cggccaccac ctgaacccgg tcagcgcctg accactggcc
     2881 gccgttaatt ttccgggcga tctggttgag gttgttcccc atcccggcaa gctggcgcag
     2941 caaaaccgga tcgatggacg gcagccgcaa cgaccgcgcc gggcgcgtat cgagacaggt
     3001 ttcccgcatc cacaccgcca gttgccttcc attgcagcgc tccagcagct gctggtactc
     3061 cccatcggtc acccggatac ctaaaaaacg ggtacgctta accgccatcc tctcctccga
     3121 tatccgcaaa atgtgaccgg gctgaacgct caagctctgc catctgctcc cggcggtaat
     3181 cttcctcctg tgcctgacgc atcagatttg ccatcactgc cggatgccag gatctttttt
     3241 ttacgacgag cggcttcttc ttcggcttct gcattatccc tccttcgcaa acgccggacg
     3301 gcacggaaaa gttttccggg tcgctccggc gttggacaag cgccagcgcg tcagtggtgt
     3361 tagcgggttt cggggcgcag ccctgaacca gtcacagcgt ttacgctgtg tatactggct
     3421 tactatgttg gcatgagagc acgattcatg aaagtgcctc atgtggcagg tgtgatatgt
     3481 ggctcaggag cgtccgcaat accatgcacg gacagatgaa atgacgcctc ctcgctcact
     3541 cggtagctgc gctcctgccg ttcggctgcg gcgggcgtga ccgactcacg gataacggga
     3601 taaagcaaag gcgctcaaat cagaaagggc cggagaaaag gatgcggcgt tgccgttttt
     3661 ccataggctc cgcctcccct gacgagcatc acagaaatgg acgctcaagt cagaggagga
     3721 cgaaacccga caggacttaa agatgccggt gatttctctc cggcggctcc ctcgtgcgct
     3781 ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttttccc tttcgggaag
     3841 cgtggcgctt tctcatagct cacgctgctg gtatctcggc tcggtttagg tcgttcgctc
     3901 caagctgggc tgtatgcacg aaccccccgt tcagcccgac tgctgcgccc tttccggtaa
     3961 ctatcatctt gagtccaacc cggacggaca cgcaaaaacg ccactggcag cagccattgg
     4021 taactggata tatacgagag agatattgag aggtcttgaa gtgggtgcct aactgcggct
     4081 acactgaaag aacagttttg gtatctgcgg tctcgtatac ccagttaccc ggttaagcag
     4141 ttccccaact gacttaacct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag
     4201 tgcaagagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttaaat gatcgtcagt
     4261 tctgttatcc ccggagctcc atccatccca cggaaaaaca cccgccactg atgccctcag
     4321 cgcactgcac atcatgtaca aaccactccc tccctccttt tttattgcgt ttcttaatgc
     4381 gcaatggtag ctttgtgcgc aacaaaaaac gcaatagcaa gggggggtaa tgagagaaag
     4441 aacaaatatc atgctagata taggattaaa gcaatcctta caacggcttt cgaataaaac
     4501 caaaatgagt ctaagtgatc ttattaacca gagattatca ggttcacttt cagttgaaga
     4561 acgcattggc gaaaagcctg aatggatgag aaaccttgat ccctatctcg ccaatcactt
     4621 ttcaaatatt gagttaccgc tcccttcacc attaaaagaa taccaagaaa aaatggatga
     4681 gatagattca attattaatg acatgatggt tctaaaggaa tatttcagag ctaagtttta
     4741 cgacgaaata aaataggcac gctatacgga ggcggagaat tatgtttttt aaacggtatt
     4801 taacggagaa ggaaatatac aaggcagcac tcccaattgt taggcttcaa gggagagtta
     4861 acacttcatt acgtttttta tccaaatact cagatgtaaa caaaaatgga ttaattgcat
     4921 tgcatcttaa tttattacat tttatctcat acaacataca cggtgtagag tctctgaaaa
     4981 tcactcaagc aggcataaac aaagctgtac aacaaagccc tcacgcagat gaaataaaaa
     5041 atattgcgca ggcaatagga cgcaaaggac ctgagtacat cgaaggatta attagaaacc
     5101 catgggggaa gaaaaatgat aatccactaa tatttataat gagcattgaa gaaagtataa
     5161 aagaaaccct taactcaggt tatatgttat atagagtagc tcccataact gagggtgaag
     5221 cagctgcttt catggatgtt cttaaacaat tattattatt tgaggataat tttcgccttt
     5281 ttggtagaga cttggtttat aaaatagaaa aattaaaaat cagataacaa agtatggtga
     5341 acgaaaaatg aaagagagga tagtgaaatg tgtaatacca agattctcaa ctatgtattg
     5401 gttaattgcg ttcctagcat ttatattccc cggtcgatct tttgctgacg gttacaccgc
     5461 ttactaccga tgtaatggta ctcaggaagc atctcttact gttaaagata acaaactaag
     5521 tatggttgta ggtctttcta agtttaatag gcaactggat acttatagat tcaaagaaaa
     5581 gggaatcgaa gtagta
//
