LOCUS       NZ_CP064256            11970 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JX-CR-hvKP-1 plasmid pJX1-4, complete
            sequence.
ACCESSION   NZ_CP064256
VERSION     NZ_CP064256.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16561274
            Assembly: GCF_015356055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Prevalence of the NTEKPC-I on IncFrepB plasmids among Hypervirulent
            Klebsiella pneumoniae isolates in Jiangxi Province, South China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Yong wai zheng jie No. 17, Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 06:54:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,852
            CDSs (total)                      :: 5,731
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 55 of 143
            Pseudo Genes (incomplete)         :: 90 of 143
            Pseudo Genes (internal stop)      :: 15 of 143
            Pseudo Genes (multiple problems)  :: 17 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064256.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JX-CR-hvKP-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJX1-4"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018/2019"
     gene            1..1488
                     /gene="ltrA"
                     /locus_tag="IS496_RS28945"
     CDS             1..1488
                     /gene="ltrA"
                     /locus_tag="IS496_RS28945"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            1593..3278
                     /locus_tag="IS496_RS28950"
     CDS             1593..3278
                     /locus_tag="IS496_RS28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3288..3545
                     /locus_tag="IS496_RS28955"
     CDS             3288..3545
                     /locus_tag="IS496_RS28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3630..3779
                     /locus_tag="IS496_RS28960"
     CDS             3630..3779
                     /locus_tag="IS496_RS28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5263..7227
                     /locus_tag="IS496_RS28965"
     CDS             5263..7227
                     /locus_tag="IS496_RS28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7227..7958
                     /gene="mobC"
                     /locus_tag="IS496_RS28970"
     CDS             7227..7958
                     /gene="mobC"
                     /locus_tag="IS496_RS28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7965..8495
                     /locus_tag="IS496_RS28975"
     CDS             7965..8495
                     /locus_tag="IS496_RS28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8522..8701)
                     /locus_tag="IS496_RS28980"
     CDS             complement(8522..8701)
                     /locus_tag="IS496_RS28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8727..9155)
                     /locus_tag="IS496_RS28985"
     CDS             complement(8727..9155)
                     /locus_tag="IS496_RS28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9319..9633)
                     /locus_tag="IS496_RS28990"
     CDS             complement(9319..9633)
                     /locus_tag="IS496_RS28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9621..9899)
                     /locus_tag="IS496_RS28995"
     CDS             complement(9621..9899)
                     /locus_tag="IS496_RS28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(10074..10439)
                     /locus_tag="IS496_RS29375"
     CDS             complement(10074..10439)
                     /locus_tag="IS496_RS29375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(10436..10807)
                     /gene="tolA"
                     /locus_tag="IS496_RS29005"
     CDS             complement(10436..10807)
                     /gene="tolA"
                     /locus_tag="IS496_RS29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(11081..11326)
                     /locus_tag="IS496_RS29010"
     CDS             complement(11081..11326)
                     /locus_tag="IS496_RS29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..8278
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3883..4461)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7228..7955
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gtgatgtacg gagaggagaa gtcggactcg ctcatagtag cggcgaagca ggcgaacaac
       61 ccgaaaggag cggagtcagt ggagcgaagg agcggggcca aggggaacgc ggaacagcca
      121 cacatgcgcc ggacacagag ccgggaaagc atgtcacaga ggctgtcacg cgtgcgggaa
      181 gctgcgaagc agcggaagaa agaacggttt acagcattgt tccacctgct gacagtcgaa
      241 gcactggaag ccgcattcct ctccctgagc aggaaagcgg ccgccggagt ggatggcatc
      301 aggtggatgg actacgccgg aaacatgaag aacaacataa cagatctgca ccggaggcta
      361 catcagggca gctacagggc gcagcccggc aggcgtcact acatcccaaa agcggatgga
      421 aaacaacgcc cgctcggcat cgcctcgctg gaggacaaga tcgtccagta tgcgctggtg
      481 aaaatcctga acgcagtcta tgaaaacgac tttatggggt tctcatacgg gttcagaccc
      541 gggcgaagcc agcacgatgc actggacgca ctggccacag ggctggtacg cactaacgta
      601 aactgggtac tggatgccga catcagtcag ttcttcgaca gggtgagcca cgaatggctg
      661 atcaggttca cagagcatcg gatcggcgac cggagggtaa tcaggctcat acgtaagtgg
      721 ctcacagccg ggacgtcgga ggagggtcaa tggcgagcaa cggaggaagg caccccacag
      781 ggtgcggtca tctcaccgct gctggcaaac atatacctcc actacgtctt cgatctgtgg
      841 gcgcatcagt ggcgacgtcg ctatgccaca ggcaatgtgg taatggtcag atacgccgat
      901 gacatcgtca tcgggttcga caaacgatac gatgcccggc gcttccgtat agccatgcag
      961 cgcagactga gggagttcgg actcacggtt cacccggaga aaacccgtct gatggagttc
     1021 ggccgcttcg ctgccgaaaa ccgtgccatc aggggaaaag gcaaaccaga aacgttcaac
     1081 ttcctcgggt tcacgcacat cagcgggaaa gatcgcaacg gcaggttcat gctgatacga
     1141 aagacccgcc gggatcggat gacggcaact ctgaaagcca tcaaagacgg tctgcgaagg
     1201 cgctggcatt actcaatccc cgaacaggga aaatggctca ggagagtggt tcagggatac
     1261 ctgaactatc actcggtacc gggcaacttc cccaccatgc agaagttcag gacacacgta
     1321 acaaacctct ggcgccgggc gctcaggcgc aggagccaga aggatgatac gacctggacg
     1381 aaagcaaaca aactggcagc cgcatggcta ccaagggttc gggttcttca tccatggcct
     1441 gtggagcggt tcaccgccag acacccgagg caggagcccg gtgcgtaaat cgcgcacgcc
     1501 gggatctgtg cggggggtat ccggtaacgg gtatccctac cgcgacatgt tggctttaat
     1561 ttattgtttt aaatgtcaaa gaggaaaacg gtatgagtgg tggagacggt cgaggtccgg
     1621 gtaattcagg tctgggacat aatggtggtc aggccagtgg gaatgtgaac ggtacgtctg
     1681 gtaaaggtgg cccttcatca ggtggtggta cggatccaaa cagcgggccg ggctggggta
     1741 cgacgcatac gccgaatgga gatattcata actataatcc gggggagttt ggtaacggcg
     1801 ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca tagcggtagc tctggtggcg
     1861 gacagtcttc ggccactgcg atggccttcg gtctgcctgc cctggctact ccgggggctg
     1921 aaggactggc tttatcagtt tccggcgatg cgttgtcggc cgccgttgct gatgtgctgg
     1981 ctgccctgaa ggggccgttt aagtttggtc tgtgggggat tgcgatctac ggtgtgctgc
     2041 cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa aattatgacg tcattaccgg
     2101 ccgatgcggt gacggagact ccggcaagta ctttaccact ggaccaggcg acggttcgtg
     2161 tcagacaacg ggttgtggat gtggtgaagg atgagcggca gcatattgcg gttgtcgcag
     2221 gtcggccaat gagtgtccct gtggtggatg cgaaaccgac aaaacgtccg ggggtattca
     2281 gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt accgaaaggt gttccgacag
     2341 cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga ttcacgtccg gctggtttta
     2401 cagccggtgg taactcccgt gaggccgtta ttcgtttccc gaaagagacc ggacagaagc
     2461 cggtttatgt gtcggtgaca gatgttctta ccccggcaca ggtaaaacag cgtcaggagg
     2521 aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc ggaagagggg ctgaaaagag
     2581 actatgataa agcgaaagct gagctggatg ccgaagataa aaatattgcg accttaaaca
     2641 gccgcattgc atcgacagag aaggcgctcc ccggtgcaag ggctgctgtt caggaagccg
     2701 ataaaaaggt aaaagaggca gaggcaaata aagatgattt tgtgacttat aacccgcctc
     2761 atgaatatgg ctccgggtgg caggatcagg ttcgctatct tgataaggat attcagaatc
     2821 agaatgagaa attaaaagcg gctcaggcat ctttaaacgc aatgaatgaa tccttatcca
     2881 gagataaggc tgcacttacc ggggcgatgg agagccggaa acaaaaggag aaaaaagcga
     2941 aggatgcaga aaataagtta aatgaggaaa agaacaagcc tcgcaagggg actaaagact
     3001 acggccatga ttatcatcca gtacctaaaa cagaagacat aaagggactt ggagagttaa
     3061 agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg taagcgggcc cgatggtatg
     3121 gcgataaaaa acgtaaaatt tatgaatggg attcccagca cggtgagctt gaagggtatc
     3181 gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa aacgggtaag caggttaaag
     3241 ggccagatcc aaaacggaac attaaaaaat atctttgaga ggtaaatatg gggcttaaat
     3301 tacgtttgac atggtttgat aagaaaactg aagagtttaa aggggaagag tattctaaag
     3361 actttggtga cgatggttct gtcattgaaa gcttgggaat gcctttaaag gataatatta
     3421 ataatggtga gtttgatttg aagaaagaat gggttccttt actacagcca ttttttaaaa
     3481 atcaaataaa acctgatgtg gataattgtt atatagcttt tgattatcgg gacggtaact
     3541 ggtaacaaaa aattatcaag gatgatttga aatactgact tccggtgttc agggatgaat
     3601 gccggagtct ttaaatacag ggaaagtata tgaaaaaggt aaagactatt tttcttttta
     3661 tattgattgt tgctggcttt cttctggccg catgtcaggc aaattatatc cgggatgttc
     3721 agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg gttcagtaga
     3781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctttt gccctgtaaa
     3841 cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg gaactgctta
     3901 accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca gtgtagccgg
     3961 actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc
     4021 ccagttacca atggctgctg ccagtggcgt tttgcgtgct ttcccgggtt ggactcaagt
     4081 gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg cttacagccc
     4141 agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta tgagaaagcg
     4201 ccacacttcc cgcaagggag aacggcggac aggtatccgg taaacggcag ggtcggaaca
     4261 ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt cctgtcgggt
     4321 ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg caccgaaaaa
     4381 gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg ccaccggcgc
     4441 ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc tgtgggtaac
     4501 tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg accgagtgag
     4561 cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct gatgtgggat
     4621 attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat aggtgaacca
     4681 tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt tctccttgcg
     4741 gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag accttgctta
     4801 cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct gtccggctgg
     4861 acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa
     4921 aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg
     4981 gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg
     5041 gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc
     5101 ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg caatcaccac
     5161 ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag cgggggctgc
     5221 gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg
     5281 cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg
     5341 ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct gtttttcggt
     5401 ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa ggcgcggcgg
     5461 gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc ggtgctggcc
     5521 tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa ctacgcgtac
     5581 tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     5641 gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga ttattacctg
     5701 ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt cgccggcgtc
     5761 cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc cgctgacgag
     5821 gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac cggagaaatg
     5881 acagacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt
     5941 tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag
     6001 ccggtgctgg tccctcgtga tacctggcgc aagaccaata ttcagatcct cggcctgccc
     6061 gggagcggca agagcgcgat ggccaccaac gcgctcattc gctgcgtacg cgatttcggg
     6121 gacgctgtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg
     6181 cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     6241 ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt
     6301 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     6361 gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc gtacgcgttg
     6421 ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac
     6481 ctgagcgtcc tgcagacgga cagcggtatc gatgtggcgg ggataatcaa cggcggcggc
     6541 tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg
     6601 tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg
     6661 agcatcatgc tcgatgagat taaatatctg ctgtcgaagt acgtgctgaa cgcgctgggg
     6721 acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggt
     6781 cagtgcgggc aggatctgcc cgctgatttt gtgaaaacca cggtactgga taacacgccg
     6841 atccgctggt tctatcgcgc ggccagccag gagtccgcgc agtgggcggc gggccagacc
     6901 ggggagatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag
     6961 cacatcagcg gcgacagctt catccagaag gaggcgcggc cgctgtttga cgtgaacacc
     7021 ctgcaacacc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc gcggctggcg
     7081 ttcagcagcc cgctgcgggt tgagcgccgg gaaatttcgc tgaaaacctt cccggtgctg
     7141 gcgaaaacgg acccgctggc ggagtacctg cctccggccg acggcggcgg accaaaagcg
     7201 gatgatgatt ttgcggggct ttactgatga cactggagca gtacaacgtc agccacgcga
     7261 agcggcaggc gcggaacgcg gagaaaacac ggctgaccct gcgctggctg cgggaagagc
     7321 tgtgttccac ggcggagctg gttgcgcgac ggctggggat tgcggcggta cagccggttt
     7381 accgcttcct ggacagtctg gtggcgaaag ggctgctggt ccgggcgaaa tacccggttg
     7441 acggccgtca ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc tcgttcgacg
     7501 aggacgagcc gctgacggac gttattccgt ttcagccgtc aagggtgtcg gcggcgcagc
     7561 tgccgcaccg gctggccgtg cagtccctgc gcctctccat ggagggccgg ggggccaccg
     7621 gctggcggta cctgcaccgt atggccctga aagggatgaa agtgccggac gcgctggcgg
     7681 agctggacgg cagaacggtg gcatttgagg tagagcgcac cgtcaagagc cgcaggcggt
     7741 atcaggaagt ggtgtccggg tatctgttca accgccgggc gaacggcatc gatgaaatct
     7801 ggtacatctg ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg
     7861 atgaaatcgt gaatccacag accggcgagg cccgcaaaac ggtggagctg gaccgggaga
     7921 ggctgttcgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg ctgtacatgg
     7981 tatttattga ggacgtggaa gacaaagacg gcgagcgctg cattatgcca accagcgagc
     8041 atctggaaga catggtggtg ctcgcctggc agaccggggc gtatgttttc tgttttgtcg
     8101 accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag cggctcaaca
     8161 gcgacgaaaa agccacgtac tttttcaccg gtgaaaatcc gcaatgggaa cgggacaaac
     8221 ccacaggaat gaagctgtgt gattacctct atcgaaaggt ggagcggttc gccatcgaga
     8281 acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac gcgatgattg
     8341 aggtggtcag cgagcggcac ggggaaagcg cggtgaacga gaagctgaaa gcggagctga
     8401 tgcgggaata taacagccgc tattttaacg tgccggtgcc tgaactgccg gaggacaaac
     8461 ccgcccgccg gtggtggccg ttcggcaaaa aataacgcca gcaccggcat ggccggtgct
     8521 gttattcttc cccgagctta tcccgcatca cctgccagag tgtttccgca tcctcgtgaa
     8581 gccgttcgca catgtcggcc ggggtatcca tgtccagctc gttcagcttt tccagcaaca
     8641 gcagcgactg atccttaatg aatttagcca tattgagggc agttttttcc tgcttcgtca
     8701 tgttctccat tcccttcggt aagttctcag ctaaaccagc cggaccagaa gcgttttttc
     8761 ttctgtgtca gttctgttgt caatctgtcc acttcggcct ggagtttccg gcgctcatca
     8821 tcgtgctggc ggcgttcttc ttcccgggat gttttatctt ccagcatcag ggtgacagcc
     8881 tgccggagat cggccagttc cttttgcatt gccagaagct gttcctgtgg gaaatttttt
     8941 tgtgtatttt cacggtgtga aatgccggga atttgtttct cagggatatt cacatttccg
     9001 tatacacgga tcagttctga cacatccacg acagggttgt ttttcccgtc acgtgacacg
     9061 gtaacctttc cctgttttac atgattgtat agcgttcttc tggtaatgcc cgcggcttgt
     9121 gctgcctgtg aaaggttgag caatgttttc gccactgtat acccctgatt ttatcgggtg
     9181 tgaaaggtgt gtaatttctc acggtaaatg tatcgctgaa ttttacccgt ttttccgttc
     9241 aggatgtggt tttctgacgc gctgcgcttg tcaaaccggc gacggccacg caatgaattg
     9301 cgcccccgtc atcggttatt agaggctgtc tgcccccgga tacccggatt ctttctgatg
     9361 cctgacggtg acgatcacaa tctggtccat ttcccggtcg tggcggtaga gcatcacata
     9421 gccactgtcg ccaaacccga tcaccagttc cttatattcc agcggcagaa acggcaccgg
     9481 gcggccaatg tctggcaatg ttttcagttg ctggatagcc cggacgatca cctcacctcc
     9541 ttttctggct gccagccgat ttttggtttt gagaaagtcc tgaaggcgtt gtaaatcgcg
     9601 ctgtgccagc gcggaaatcg ttacctgtgg catggcggag catcctgctc gttctcagtt
     9661 ccccaggtac ttatccaggc ttccgcttct tccgcagtaa ggtgcagacc cgtttcctga
     9721 tagtgctgcc atgcggcctg tccgtcccgc aggtactggc tgcgcttctc ttcccggtcg
     9781 atgtattcgg tgattgcctc cagcattagc gcgtgcgctg agcggtgccg gtcatcggcc
     9841 agcgttttca gccggtcctt aagttcttca tcgagcctga tggatgttgc tgatgacata
     9901 aaagcccctt tgtagtcata tgtattacac atgactactt taatccgtgg cggcgctatt
     9961 tgccagaaat acggcttttg ctgcccattt ttcagcttcg cttccactaa tcagggctgc
    10021 tacaggctca ctcatcatcc aggactattg cagcctgcgg gttaaaggac ctgttactgg
    10081 cggcaaccat catcattgag cttttgggtg gtgactggtc gcggatcctg taagccgtca
    10141 tttttcagac tgaattcaaa atttataacc tgtcgcattg ccgggtgccc tgcgggatat
    10201 agccagtgcc tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac
    10261 acgttgtcca ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg accttcgatg
    10321 cgttttgctg cggcctgtac cgggtaggtt ggctgcagtg tggttacggg ttttagtggt
    10381 atcgcatgta ccggagtgag gaacatggcc gccagcactg tcagagtaaa agctttcatg
    10441 gtctgaaatc cagaggggcg tttttgaata tttgccaggt attttcatcc ggtgccggcg
    10501 gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt
    10561 cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt
    10621 ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag
    10681 cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg
    10741 aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta
    10801 aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta
    10861 cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc
    10921 catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg
    10981 ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt
    11041 ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct
    11101 gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc
    11161 tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg
    11221 cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt
    11281 ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc
    11341 gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag
    11401 atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg
    11461 ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt
    11521 gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta
    11581 ataattatac tgtgtatatg tacagtgtgc gccgggagac cggttagaga tcaaggggtg
    11641 aaagtccccg accattgaag gaccagcaat ccacaaggtc cccgagtcat gcgttgcata
    11701 ccgcgaggta tggggcgaag cgttgacagg ggtgttgaca ggccagccat tgagccacga
    11761 aatgtatatt aaattaccgg gtgccgacgt tgtactgtta acggaaggca acatcatagg
    11821 gtgcgatact gcgagtgcca catggacccg gcggggtctg agaccctggc atgtcaatac
    11881 gatctctacg cgggaaccgg gagatctccc ctctgaccat ctgccagtgt cggagatggc
    11941 ccgcaccggg aagacgagga gtcatagccg
//
