LOCUS       NZ_CP064240             5596 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JX-CR-hvKP-4 plasmid pJX4-5, complete
            sequence.
ACCESSION   NZ_CP064240
VERSION     NZ_CP064240.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16561284
            Assembly: GCF_015355995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Prevalence of the NTEKPC-I on IncFrepB plasmids among Hypervirulent
            Klebsiella pneumoniae isolates in Jiangxi Province, South China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Yong wai zheng jie No. 17, Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 259x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 09:31:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,009
            CDSs (total)                      :: 5,887
            Genes (coding)                    :: 5,698
            CDSs (with protein)               :: 5,698
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 70 of 189
            Pseudo Genes (incomplete)         :: 128 of 189
            Pseudo Genes (internal stop)      :: 24 of 189
            Pseudo Genes (multiple problems)  :: 33 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064240.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JX-CR-hvKP-4"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJX4-5"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018/2019"
     gene            1..510
                     /locus_tag="IS499_RS29770"
     CDS             1..510
                     /locus_tag="IS499_RS29770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            517..729
                     /locus_tag="IS499_RS29775"
     CDS             517..729
                     /locus_tag="IS499_RS29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1396..1620
                     /locus_tag="IS499_RS29780"
     CDS             1396..1620
                     /locus_tag="IS499_RS29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1604..1870
                     /locus_tag="IS499_RS29785"
     CDS             1604..1870
                     /locus_tag="IS499_RS29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1940..2290)
                     /locus_tag="IS499_RS29790"
     CDS             complement(1940..2290)
                     /locus_tag="IS499_RS29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2356..2901)
                     /locus_tag="IS499_RS29795"
     CDS             complement(2356..2901)
                     /locus_tag="IS499_RS29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2927..3253)
                     /locus_tag="IS499_RS29800"
     CDS             complement(2927..3253)
                     /locus_tag="IS499_RS29800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3611..3769)
                     /locus_tag="IS499_RS30375"
     CDS             complement(3611..3769)
                     /locus_tag="IS499_RS30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4409..4585
                     /locus_tag="IS499_RS29805"
     CDS             4409..4585
                     /locus_tag="IS499_RS29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4575..4919
                     /locus_tag="IS499_RS29810"
     CDS             4575..4919
                     /locus_tag="IS499_RS29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5101..5346)
                     /locus_tag="IS499_RS30155"
     CDS             complement(5101..5346)
                     /locus_tag="IS499_RS30155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4244..4323
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3661..4058)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4..845
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.644"
                     /coverage="99.0"
                     /evalue="6.52e-159"
     Typing          4910..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.62e-147"
ORIGIN
        1 atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag
       61 agtaccggcg aaatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa
      121 ctgagcgaaa gcgggaagag aatcagcgcc gccatccgcg cccacgagca gggtatgagc
      181 gcggccatgc agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac atggctgacc
      241 atcacgatgg tgtcaggact gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg
      301 agcttgatag cgtcaaatct ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg
      361 aacgcgaaga cctggggcgt gacgtacctg gaagacagca acggacgttt tctggtgctg
      421 ccgaagggga cggccgtaga caggacgcag agctggacgg tcgggaacgg gcggtcgaag
      481 cagaacgcgc tgagactggt gaaggagtaa gtgccgatga ccgagctgga aaaacagttg
      541 ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc
      601 gccttcgggg aatggcggaa aatgtcaggg cttatgcaac gggagaacgc ggcgctgagc
      661 gagcgcgtga cgcgcttgag tcagcaggtg gaacgcttga gcgggcaact gcagcgtttg
      721 agccggtagt gcagcgtcat gagatggccg tggccgcaga acgggcgcac gagcaacggc
      781 agcacgaaaa ggaactgacg gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc
      841 tgggctgaaa tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa ccggatgtca
      901 ttcacctggc tgcatggcta tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt
      961 tatcgggtgg tttgttgccg gttttacctg actgccaccc cgttcgcggc gaacccgtcc
     1021 ggggcggtgc gggcaacggg tgttatgttt aatcagcgtg aacggcagat acaaaaaagc
     1081 cccggagggc ttttaactac tgacttattt ttttgaagat ttgctgggtt tcaatttgtc
     1141 agtatttccc ggcgtttttt tattgtcacg ctggcgttta tcaggttccc ctgttgaatc
     1201 ggcaattcgt ttcggacccg gcttaagacc cataatcgtt atcctttcaa tagttaagag
     1261 gaaagccctc aagctagata tattgaccgc gttacggatt atcaagaggg gctaacaaga
     1321 ttattttttg ttcttttagg ttgattgtgg tactgttgat atacatgtat agcaaatggt
     1381 gttcgggagg tttatatgct tgctatccgg ttatctgatg agattgagtc ccgtctggac
     1441 tcgctggcga agcaaaccgg cagaacaaag acgttttatg cgcgggaagc aatactggcg
     1501 catctggaag acctggagga ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt
     1561 ggtgatgaag cagtgcattc gtctgaagac gtgaggaagt cacttggtct ggacgattaa
     1621 ctattccgat cgggcgctca aatcgttacg caagatggac aaacagaacg cacgacggat
     1681 tgtggatttt atgagtttac gcattgcagt tgctgccgat cctcgccagt cagggaagcc
     1741 gctcaaaggt gagctgggcg agttctggcg ctatcgggtg ggagattatc gcgttctgtg
     1801 tgagatccga gatgacgagc ttgttatcct tgccgccacg attggacatc gccgcgaagt
     1861 ttacgactga agcccgctat gcgggctttt ttgatgccga caacaggtat tatggtaaat
     1921 actcgactaa ctacgtgttc tactgtttgt cggttttagt taaccagcag gttgtcttaa
     1981 cgataatgta gccgttattt ttaactaata aggtcatatt gttaacgtca ggcataataa
     2041 aagacattat ggcctcagag ttatcatcag aagcagctct ctgcattact acttcgattc
     2101 ccttttcttt gaatctataa gtatccagtt gcctattaaa cttagaaaga cctacaacca
     2161 tacttagttt gttatcttta acagtaagag atgcttcctg agtaccatta catcggtagt
     2221 aagcggtgta accgtcagca aaagatcgac cggggaatat aaatgctagg aacgcaatta
     2281 accaatacat agttgagaat cttggtatta cacatttcac tatcctctct ttcatttttc
     2341 gttcaccata ctttgttatc tgatttttaa tttttctatt ttataaacca agtctctacc
     2401 aaaaaggcga aaattatcct caaataataa taattgttta agaacatcca tgaaagcagc
     2461 tgcttcaccc tcagttatgg gagctactct atataacata taacctgagt taagggtttc
     2521 ttttatactt tcttcaatgc tcattataaa tattagtgga ttatcatttt tcttccccca
     2581 tgggtttcta attaatcctt cgatgtactc aggtcctttg cgtcctattg cctgcgcaat
     2641 attttttatt tcatctgcgt gagggctttg ttgtacagct ttgtttatgc ctgcttgagt
     2701 gattttcaga gactctacac cgtgtatgtt gtatgagata aaatgtaata aattaagatg
     2761 caatgcaatt aatccatttt tgtttacatc tgagtatttg gataaaaaac gtaatgaagt
     2821 gttaactctc ccttgaagcc taacaattgg gagtgctgcc ttgtatattt ccttctccgt
     2881 taaataccgt ttaaaaaaca taattctccg cctccgtata gcgtgcctat tttatttcgt
     2941 cgtaaaactt agctctgaaa tattccttta gaaccatcat gtcattaata attgaatcta
     3001 tctcatccat tttttcttgg tattctttta atggtgaagg gagcggtaac tcaatatttg
     3061 aaaagtgatt ggcgagatag ggatcaaggt ttctcatcca ttcaggcttt tcgccaatgc
     3121 gttcttcaac tgaaagtgaa cctgataatc tctggttaat aagatcactt agactcattt
     3181 tggttttatt cgaaagccgt tgtaaggatt gctttaatcc tatatctagc atgatatttg
     3241 ttctttctct cattaccccc ccttgctatt gcgttttttg ttgcgcacaa agctaccatt
     3301 gcgcattaag aaacgcaata aaaaaggagg gagggagtgg tttgtacatg atgtgcagtg
     3361 cgctgagggc atcagtggcg ggtgtttttc cgtgggatgg atggagctcc ggggataaca
     3421 gaactgacga tcatttaaag gatcttcttg agatcctttt tttctgcgcg taatctcttg
     3481 cactgtaaac gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg
     3541 aactgcttaa ccgggtaact gggtatacga gaccgcagat accaaaactg ttctttcagt
     3601 gtagccgcag ttaggcaccc acttcaagac ctctcaatat ctctctcgta tatatccagt
     3661 taccaatggc tgctgccagt ggcgtttttg cgtgtccgtc cgggttggac tcaagatgat
     3721 agttaccgga aagggcgcag cagtcgggct gaacgggggg ttcgtgcata cagcccagct
     3781 tggagcgaac gacctaaacc gagccgagat accagcagcg tgagctatga gaaagcgcca
     3841 cgcttcccga aagggaaaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg
     3901 agagcgcacg agggagccgc cggagagaaa tcaccggcat ctttaagtcc tgtcgggttt
     3961 cgtcctcctc tgacttgagc gtccatttct gtgatgctcg tcaggggagg cggagcctat
     4021 ggaaaaacgg caacgccgca tccttttctc cggccctttc tgatttgagc gcctttgctt
     4081 tatcccgtta tccgtgagtc ggtcacgccc gccgcagccg aacggcagga gcgcagctac
     4141 cgagtgagcg aggaggcgtc atttcatctg tccgtgcatg gtattgcgga cgctcctgag
     4201 ccacatatca cacctgccac atgaggcact ttcatgaatc gtgctctcat gccaacatag
     4261 taagccagta tacacagcgt aaacgctgtg actggttcag ggctgcgccc cgaaacccgc
     4321 taacaccact gacgcgctgg cgcttgtcca acgccggagc gacccggaaa acttttccgt
     4381 gccgtccggc gtttgcgaag gagggataat gcagaagccg aagaagaagc cgctcgtcgt
     4441 aaaaaaaaga tcctggcatc cggcagtgat ggcaaatctg atgcgtcagg cacaggagga
     4501 agattaccgc cgggagcaga tggcagagct tgagcgttca gcccggtcac attttgcgga
     4561 tatcggagga gaggatggcg gttaagcgta cccgtttttt aggtatccgg gtgaccgatg
     4621 gggagtacca gcagctgctg gagcgctgca atggaaggca actggcggtg tggatgcggg
     4681 aaacctgtct cgatacgcgc ccggcgcggt cgttgcggct gccgtccatc gatccggttt
     4741 tgctgcgcca gcttgccggg atggggaaca acctcaacca gatcgcccgg aaaattaacg
     4801 gcggccagtg gtcaggcgct gaccgggttc aggtggtggc cgcgctgatg gccattgatg
     4861 ccgaactcga gcggctgcgg catgccgtgc tggaaaaggg ggcagacgat gatcgttaag
     4921 tttcatccgc gagggcgagg cggcggtgcc gggccggtgg attatctgct ggggaaagac
     4981 cgccagcgcg acggggccat cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc
     5041 gacgcctcgc cctacgccaa aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat
     5101 ttaccgcccg gccagcgtga aaagctgatg gcgagcttcg agcgggttct gatgcccgga
     5161 ctcgataaag accagtacag cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa
     5221 ctgaattttc tggtaccgaa cacggaactg ctgaccggca ggcgtctcca gccgtactac
     5281 gaccgggcag accgtccgcg catcgatgcg tggcagaccg tggtgaacgg gcgactgggg
     5341 ctgcacgacc cgaacgcacc ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc
     5401 aaaacgaagc aggaagccgc agaggctatt acgcggggct tactggccct cgcctcgtca
     5461 ggggagctta aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc
     5521 gtgcgcacca caaagagcag catcagcatt gccgacccgg acggagggcg aaacatccga
     5581 cttaagggag ccatct
//
