LOCUS       NZ_CP064239            11970 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JX-CR-hvKP-4 plasmid pJX4-4, complete
            sequence.
ACCESSION   NZ_CP064239
VERSION     NZ_CP064239.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16561284
            Assembly: GCF_015355995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Prevalence of the NTEKPC-I on IncFrepB plasmids among Hypervirulent
            Klebsiella pneumoniae isolates in Jiangxi Province, South China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Yong wai zheng jie No. 17, Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 259x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 09:31:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,009
            CDSs (total)                      :: 5,887
            Genes (coding)                    :: 5,698
            CDSs (with protein)               :: 5,698
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 70 of 189
            Pseudo Genes (incomplete)         :: 128 of 189
            Pseudo Genes (internal stop)      :: 24 of 189
            Pseudo Genes (multiple problems)  :: 33 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064239.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JX-CR-hvKP-4"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJX4-4"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018/2019"
     gene            1..1686
                     /locus_tag="IS499_RS29700"
     CDS             1..1686
                     /locus_tag="IS499_RS29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1696..1953
                     /locus_tag="IS499_RS29705"
     CDS             1696..1953
                     /locus_tag="IS499_RS29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2038..2187
                     /locus_tag="IS499_RS29710"
     CDS             2038..2187
                     /locus_tag="IS499_RS29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3671..5635
                     /locus_tag="IS499_RS29715"
     CDS             3671..5635
                     /locus_tag="IS499_RS29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5635..6366
                     /gene="mobC"
                     /locus_tag="IS499_RS29720"
     CDS             5635..6366
                     /gene="mobC"
                     /locus_tag="IS499_RS29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6373..6903
                     /locus_tag="IS499_RS29725"
     CDS             6373..6903
                     /locus_tag="IS499_RS29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6930..7109)
                     /locus_tag="IS499_RS29730"
     CDS             complement(6930..7109)
                     /locus_tag="IS499_RS29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7135..7563)
                     /locus_tag="IS499_RS29735"
     CDS             complement(7135..7563)
                     /locus_tag="IS499_RS29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7727..8041)
                     /locus_tag="IS499_RS29740"
     CDS             complement(7727..8041)
                     /locus_tag="IS499_RS29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8029..8307)
                     /locus_tag="IS499_RS29745"
     CDS             complement(8029..8307)
                     /locus_tag="IS499_RS29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8482..8847)
                     /locus_tag="IS499_RS30150"
     CDS             complement(8482..8847)
                     /locus_tag="IS499_RS30150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8844..9215)
                     /gene="tolA"
                     /locus_tag="IS499_RS29755"
     CDS             complement(8844..9215)
                     /gene="tolA"
                     /locus_tag="IS499_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9489..9734)
                     /locus_tag="IS499_RS29760"
     CDS             complement(9489..9734)
                     /locus_tag="IS499_RS29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10379..11866
                     /gene="ltrA"
                     /locus_tag="IS499_RS29765"
     CDS             10379..11866
                     /gene="ltrA"
                     /locus_tag="IS499_RS29765"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6686
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2291..2869)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5636..6363
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgagtggtg gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag
       61 gccagtggga atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg
      121 gatccaaaca gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac
      181 tataatccgg gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc
      241 ggcaatcata gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggccttcggt
      301 ctgcctgccc tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg
      361 ttgtcggccg ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg
      421 tgggggattg cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg
      481 atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact
      541 ttaccactgg accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat
      601 gagcggcagc atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg
      661 aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag
      721 gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa
      781 aaaggtgatt cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt
      841 cgtttcccga aagagaccgg acagaagccg gtttatgtgt cggtgacaga tgttcttacc
      901 ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc
      961 gctcatccgg aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc
     1021 gaagataaaa atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc
     1081 ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa
     1141 gatgattttg tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt
     1201 cgctatcttg ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct
     1261 ttaaacgcaa tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag
     1321 agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag
     1381 aacaagcctc gcaaggggac taaagactac ggccatgatt atcatccagt acctaaaaca
     1441 gaagacataa agggacttgg agagttaaag cgaggtgatc ctaaaacacc taaacaaggt
     1501 ggtggtggta agcgggcccg atggtatggc gataaaaaac gtaaaattta tgaatgggat
     1561 tcccagcacg gtgagcttga agggtatcgt gccagtgatg gcgaacacct aggcgcattt
     1621 gatcccaaaa cgggtaagca ggttaaaggg ccagatccaa aacggaacat taaaaaatat
     1681 ctttgagagg taaatatggg gcttaaatta cgtttgacat ggtttgataa gaaaactgaa
     1741 gagtttaaag gggaagagta ttctaaagac tttggtgacg atggttctgt cattgaaagc
     1801 ttgggaatgc ctttaaagga taatattaat aatggtgagt ttgatttgaa gaaagaatgg
     1861 gttcctttac tacagccatt ttttaaaaat caaataaaac ctgatgtgga taattgttat
     1921 atagcttttg attatcggga cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa
     1981 tactgacttc cggtgttcag ggatgaatgc cggagtcttt aaatacaggg aaagtatatg
     2041 aaaaaggtaa agactatttt tctttttata ttgattgttg ctggctttct tctggccgca
     2101 tgtcaggcaa attatatccg ggatgttcag ggcggaacgg tggcaccatc gtcctcctct
     2161 gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt
     2221 ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg
     2281 aaggttaagt cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca
     2341 accaaatctg tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg
     2401 tttagctaaa caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt
     2461 tgcgtgcttt cccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     2521 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     2581 ataccagtgt gtgagctatg agaaagcgcc acacttcccg caagggagaa cggcggacag
     2641 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ctaccggaaa
     2701 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     2761 tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc
     2821 ggagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc
     2881 ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc cgcagcgccc gccgcagtct
     2941 cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca atttccccgg cacctgcaag
     3001 acggtcaccg gacgcgctga tgtgggatat tgtcccctga ttaagtcttt ctattccctg
     3061 acgatcaatt atcaggatag gtgaaccatg tcggtttttc gctgaacatt ttacgttaac
     3121 ctatctctgt tgcttttttc tccttgcgga gaaatgcaag tcatccccgc tggggcgtgg
     3181 ctgacttcag gggaaaagac cttgcttacg ctggctggac gccaccgacg cacggtcttt
     3241 tcccctcttt ctggctctgt ccggctggac gccggccacg ccagaaattc acccggcccc
     3301 cgtcaggctc acgcctgacc acccacaaaa atccacggtg ctggacgcac ctgacgaagc
     3361 agcaaagctg ctcctgattt tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg
     3421 ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt ttcctctcac acgctggggc
     3481 gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc
     3541 cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc cgccgccatg ccttggggca
     3601 tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg taaacccgtc gtctgaagga
     3661 gagagattgc atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc
     3721 ccggcagatt tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt
     3781 ttttctggtt atcggcctgt ttttcggtct ggctgaactc tgggccggcc gaatgcgttt
     3841 tgtggggctg agccataagg cgcggcggga gtatgccgat cgctataagc gcgataataa
     3901 ccggactgtg ccattttcgg tgctggcctc gctggattca cccgggcgga tggtcagggc
     3961 acggcgacat gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct
     4021 gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga
     4081 cctctatgcg aacgcagatt attacctgct gcagccggct aacctgttcg ggatagcctg
     4141 gtgtgccgga gggcttgtcg ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct
     4201 tgatggtcat tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg
     4261 gcgcagcagc cagcggaccg gagaaatgac agacgtccgc cacctgcatt tcggggagcc
     4321 ggtaccggtt aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt
     4381 gtttctgggt aaggatgagc agggccagcc ggtgctggtc cctcgtgata cctggcgcaa
     4441 gaccaatatt cagatcctcg gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc
     4501 gctcattcgc tgcgtacgcg atttcgggga cgctgtggtg tacttcgacc ccaaagggga
     4561 cgcgtgggcg ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg
     4621 gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa
     4681 tctgctggtt gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat
     4741 cagcgagcag aaagcggcga agctgattgc ggagcagttc ccgcaggggg caaacattca
     4801 gcaggtgctg gcggcggcgt acgcgttgcc ggaggccctg aaaaaggacg tgaaggggct
     4861 gatcaccaag ctggagaacg tggccgacct gagcgtcctg cagacggaca gcggtatcga
     4921 tgtggcgggg ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga
     4981 ggcggtgatc cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg
     5041 ggatgagttc cggaggtggc cgcacgcgag catcatgctc gatgagatta aatatctgct
     5101 gtcgaagtac gtgctgaacg cgctggggac gctgcgcagc cgggactgca acctgctgct
     5161 cgcgcaccag tcgctgggcg acttcggtca gtgcgggcag gatctgcccg ctgattttgt
     5221 gaaaaccacg gtactggata acacgccgat ccgctggttc tatcgcgcgg ccagccagga
     5281 gtccgcgcag tgggcggcgg gccagaccgg ggagatccgg gtggacgtgg agcgccgccg
     5341 ggccagccgg gaggcgggga acgtggagca catcagcggc gacagcttca tccagaagga
     5401 ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg cctgacgggt tcgcggtgat
     5461 gaccgggctg ggcgtggcgc ggctggcgtt cagcagcccg ctgcgggttg agcgccggga
     5521 aatttcgctg aaaaccttcc cggtgctggc gaaaacggac ccgctggcgg agtacctgcc
     5581 tccggccgac ggcggcggac caaaagcgga tgatgatttt gcggggcttt actgatgaca
     5641 ctggagcagt acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg
     5701 ctgaccctgc gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg
     5761 ctggggattg cggcggtaca gccggtttac cgcttcctgg acagtctggt ggcgaaaggg
     5821 ctgctggtcc gggcgaaata cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg
     5881 ccgcacgggg tggcgttctc gttcgacgag gacgagccgc tgacggacgt tattccgttt
     5941 cagccgtcaa gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc
     6001 ctctccatgg agggccgggg ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa
     6061 gggatgaaag tgccggacgc gctggcggag ctggacggca gaacggtggc atttgaggta
     6121 gagcgcaccg tcaagagccg caggcggtat caggaagtgg tgtccgggta tctgttcaac
     6181 cgccgggcga acggcatcga tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc
     6241 cgggtacagc gggcgattct ggcggtggat gaaatcgtga atccacagac cggcgaggcc
     6301 cgcaaaacgg tggagctgga ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg
     6361 gagtgaggga ttatggcgct gtacatggta tttattgagg acgtggaaga caaagacggc
     6421 gagcgctgca ttatgccaac cagcgagcat ctggaagaca tggtggtgct cgcctggcag
     6481 accggggcgt atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc
     6541 atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcaccggt
     6601 gaaaatccgc aatgggaacg ggacaaaccc acaggaatga agctgtgtga ttacctctat
     6661 cgaaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc
     6721 tcaccggagc agattaacgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg
     6781 gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg
     6841 ccggtgcctg aactgccgga ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa
     6901 taacgccagc accggcatgg ccggtgctgt tattcttccc cgagcttatc ccgcatcacc
     6961 tgccagagtg tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg
     7021 tccagctcgt tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata
     7081 ttgagggcag ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct
     7141 aaaccagccg gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac
     7201 ttcggcctgg agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt
     7261 tttatcttcc agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc
     7321 cagaagctgt tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat
     7381 ttgtttctca gggatattca catttccgta tacacggatc agttctgaca catccacgac
     7441 agggttgttt ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag
     7501 cgttcttctg gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc
     7561 cactgtatac ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta
     7621 tcgctgaatt ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc
     7681 aaaccggcga cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg
     7741 cccccggata cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt
     7801 cccggtcgtg gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct
     7861 tatattccag cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct
     7921 ggatagcccg gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga
     7981 gaaagtcctg aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca
     8041 tggcggagca tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc
     8101 cgcagtaagg tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag
     8161 gtactggctg cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc
     8221 gtgcgctgag cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc
     8281 gagcctgatg gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat
     8341 gactacttta atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt
     8401 cagcttcgct tccactaatc agggctgcta caggctcact catcatccag gactattgca
     8461 gcctgcgggt taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt
     8521 gactggtcgc ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg
     8581 tcgcattgcc gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg
     8641 aaaaaaaagg tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc
     8701 aaacgtggcg gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg
     8761 ctgcagtgtg gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc
     8821 cagcactgtc agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt
     8881 tgccaggtat tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct
     8941 gctttacaga gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt
     9001 tgtatggata tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc
     9061 atttcaacag cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga
     9121 gtataaattt tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg
     9181 cacaatgcca gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa
     9241 atgttacatg tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat
     9301 cgtatcgttt ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt
     9361 tacaatttac cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc
     9421 gtagccgccg gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta
     9481 aatacccctt accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat
     9541 ttctcccgac gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc
     9601 accctttccc gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc
     9661 atggccagtg cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt
     9721 ttcaggtgcg tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg
     9781 tcctctcctc atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga
     9841 tgaatgtctt aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct
     9901 gcttacaggt ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt
     9961 gacacctgaa aacgggagga gtaaggtaat aattatactg tgtatatgta cagtgtgcgc
    10021 cgggagaccg gttagagatc aaggggtgaa agtccccgac cattgaagga ccagcaatcc
    10081 acaaggtccc cgagtcatgc gttgcatacc gcgaggtatg gggcgaagcg ttgacagggg
    10141 tgttgacagg ccagccattg agccacgaaa tgtatattaa attaccgggt gccgacgttg
    10201 tactgttaac ggaaggcaac atcatagggt gcgatactgc gagtgccaca tggacccggc
    10261 ggggtctgag accctggcat gtcaatacga tctctacgcg ggaaccggga gatctcccct
    10321 ctgaccatct gccagtgtcg gagatggccc gcaccgggaa gacgaggagt catagccggt
    10381 gatgtacgga gaggagaagt cggactcgct catagtagcg gcgaagcagg cgaacaaccc
    10441 gaaaggagcg gagtcagtgg agcgaaggag cggggccaag gggaacgcgg aacagccaca
    10501 catgcgccgg acacagagcc gggaaagcat gtcacagagg ctgtcacgcg tgcgggaagc
    10561 tgcgaagcag cggaagaaag aacggtttac agcattgttc cacctgctga cagtcgaagc
    10621 actggaagcc gcattcctct ccctgagcag gaaagcggcc gccggagtgg atggcatcag
    10681 gtggatggac tacgccggaa acatgaagaa caacataaca gatctgcacc ggaggctaca
    10741 tcagggcagc tacagggcgc agcccggcag gcgtcactac atcccaaaag cggatggaaa
    10801 acaacgcccg ctcggcatcg cctcgctgga ggacaagatc gtccagtatg cgctggtgaa
    10861 aatcctgaac gcagtctatg aaaacgactt tatggggttc tcatacgggt tcagacccgg
    10921 gcgaagccag cacgatgcac tggacgcact ggccacaggg ctggtacgca ctaacgtaaa
    10981 ctgggtactg gatgccgaca tcagtcagtt cttcgacagg gtgagccacg aatggctgat
    11041 caggttcaca gagcatcgga tcggcgaccg gagggtaatc aggctcatac gtaagtggct
    11101 cacagccggg acgtcggagg agggtcaatg gcgagcaacg gaggaaggca ccccacaggg
    11161 tgcggtcatc tcaccgctgc tggcaaacat atacctccac tacgtcttcg atctgtgggc
    11221 gcatcagtgg cgacgtcgct atgccacagg caatgtggta atggtcagat acgccgatga
    11281 catcgtcatc gggttcgaca aacgatacga tgcccggcgc ttccgtatag ccatgcagcg
    11341 cagactgagg gagttcggac tcacggttca cccggagaaa acccgtctga tggagttcgg
    11401 ccgcttcgct gccgaaaacc gtgccatcag gggaaaaggc aaaccagaaa cgttcaactt
    11461 cctcgggttc acgcacatca gcgggaaaga tcgcaacggc aggttcatgc tgatacgaaa
    11521 gacccgccgg gatcggatga cggcaactct gaaagccatc aaagacggtc tgcgaaggcg
    11581 ctggcattac tcaatccccg aacagggaaa atggctcagg agagtggttc agggatacct
    11641 gaactatcac tcggtaccgg gcaacttccc caccatgcag aagttcagga cacacgtaac
    11701 aaacctctgg cgccgggcgc tcaggcgcag gagccagaag gatgatacga cctggacgaa
    11761 agcaaacaaa ctggcagccg catggctacc aagggttcgg gttcttcatc catggcctgt
    11821 ggagcggttc accgccagac acccgaggca ggagcccggt gcgtaaatcg cgcacgccgg
    11881 gatctgtgcg gggggtatcc ggtaacgggt atccctaccg cgacatgttg gctttaattt
    11941 attgttttaa atgtcaaaga ggaaaacggt
//
