LOCUS       NZ_CP064193             8970 bp    DNA     circular CON 20-APR-2024
DEFINITION  Acinetobacter baumannii strain X4-65 plasmid pX4-65-1, complete
            sequence.
ACCESSION   NZ_CP064193
VERSION     NZ_CP064193.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16560991
            Assembly: GCF_029814975.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Hsieh,Y.C., Wu,J.W., Chen,Y.Y., Quyen,T.L.T., Liao,W.C., Li,S.W.,
            Chen,Y.C. and Pan,Y.J.
  TITLE     An Outbreak of tet(X6)-Carrying Tigecycline-Resistant Acinetobacter
            baumannii Isolates with a New Capsular Type at a Hospital in Taiwan
  JOURNAL   Antibiotics (Basel) 10 (10), 1239 (2021)
   PUBMED   34680819
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Pan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2020) Department of Microbiology and Immunology,
            China Medical University, Taichung, Taiwan, No.91 Hsueh-Shih Road,
            Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye (v2.7) v. Flye (v2.7)
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 295x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029814975.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:43:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,960
            CDSs (total)                      :: 3,864
            Genes (coding)                    :: 3,767
            CDSs (with protein)               :: 3,767
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 48 of 97
            Pseudo Genes (internal stop)      :: 20 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064193.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="X4-65"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pX4-65-1"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.99290100 N 121.30097200 E"
                     /collection_date="2020"
                     /collected_by="Chang Gung Memorial Hospital"
     gene            join(7348..8970,1..789)
                     /gene="znuD2"
                     /locus_tag="IS507_RS00005"
     CDS             join(7348..8970,1..789)
                     /gene="znuD2"
                     /locus_tag="IS507_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            1094..1555
                     /locus_tag="IS507_RS00010"
     CDS             1094..1555
                     /locus_tag="IS507_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_279446692.1"
                     /translation="MKLAEALLLRSDQHKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(1878..2051)
                     /locus_tag="IS507_RS00015"
     CDS             complement(1878..2051)
                     /locus_tag="IS507_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(2051..2422)
                     /locus_tag="IS507_RS00020"
     CDS             complement(2051..2422)
                     /locus_tag="IS507_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(2694..2948)
                     /locus_tag="IS507_RS19840"
     CDS             complement(2694..2948)
                     /locus_tag="IS507_RS19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            3632..4582
                     /gene="repM"
                     /locus_tag="IS507_RS00025"
     CDS             3632..4582
                     /gene="repM"
                     /locus_tag="IS507_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            4575..5150
                     /locus_tag="IS507_RS00030"
     CDS             4575..5150
                     /locus_tag="IS507_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            5170..5313
                     /locus_tag="IS507_RS00035"
     CDS             5170..5313
                     /locus_tag="IS507_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(5496..6323)
                     /locus_tag="IS507_RS00040"
     CDS             complement(5496..6323)
                     /locus_tag="IS507_RS00040"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            6632..6919
                     /locus_tag="IS507_RS00045"
     CDS             6632..6919
                     /locus_tag="IS507_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            6906..7220
                     /locus_tag="IS507_RS00050"
     CDS             6906..7220
                     /locus_tag="IS507_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     ARG             complement(5500..6323)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3633..4582
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattatatct tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg
       61 tatactcatg gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga
      121 aaagagaaat ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat
      181 caaatcagta cttactatta tgattttgat aactatatat atttacagac actcaatgaa
      241 gttttaggaa ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag
      301 tctgctgcta atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat
      361 cacggctctc tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta
      421 attgcatacg atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact
      481 cctagattac ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt
      541 aaaggtgaaa tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag
      601 gttacatcag gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac
      661 acagaatatg atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat
      721 gaaacatttt tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt
      781 aatttctaga taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa
      841 aatgattaga ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc
      901 gatttaggtt tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc
      961 agccttttca gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc
     1021 aatttttttg atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt
     1081 taataggtga aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacacaaaaa
     1141 aattatttca ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc
     1201 atctgaagat ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa
     1261 attaagttat gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct
     1321 acttgcttta ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc
     1381 aataagtaat acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa
     1441 agttattcca gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga
     1501 tttaaacgtg ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc
     1561 ctttagatat aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca
     1621 taggattgta aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag
     1681 tacaaatcaa tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta
     1741 cttataacta ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgaa
     1801 caagcatcag ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct
     1861 cttatacgaa gcccaggtta attctgggct ttttgttcaa tctcatcatt ggccgagaac
     1921 atacttggat tcgtaagcct gatattttcc tgacaacttc cacacagcat caatcctgca
     1981 aaaagttgta cattttgtct attttcacaa agcgtacatg gctttgaaag ctgaataata
     2041 ttatctgaca ctactttacc ctcttatagt tatcatctgc atatgaataa cctttagctt
     2101 gtatcattct aacctcatct ttagaagcgc catttttcat taagagccac aacatcatga
     2161 aatatagtcc agaaaagtct tcatagtatt tttcagaaaa attgttatct tcctcaaaat
     2221 catctattcg cttttctaat cgattcacca gatcctcaat acgatcggct tgctgtttaa
     2281 cagcaataga ttgaaaatca ttggtcaaaa ttttactgaa taatttactg gttctgattc
     2341 cttctgaatt agccaattga tcgtagaatt ctgctacttg tggatctaac ctgactgcat
     2401 aagttttgac tttttcgccc ataaattaca cctcttatta aaattagatt ttgtacaaaa
     2461 ttaaatatat ataattcaac taattaaata aaaaataata attagattac gtacaaactt
     2521 atttgtacag aattaattta ttctaaaaca aaaacttatt taaatataat gcttttttta
     2581 tgtacaatca tgagtacata ttttttctta aatttatcaa tatcttatct aaaaaaagtg
     2641 ttgttttgat gtacaaccat gagcacagca taaaaaatag ataattcaat cacttattct
     2701 aaaaaaagac catttttctg tacacgtttc tgagacccta aaaaatcgct gaaatcctta
     2761 tcctgtaagg ctttgagggg tgtcatcgtt agatgcgtgg tgtgtgacaa aaaaatcccg
     2821 gcatgtgccg gatcttggat tggaaaattg gctaaagtga cgtagggccg gtgcttggtt
     2881 ttgcatggaa aaaagtattt atttccttgt ttagaaaaac gtaaaaaatc aattttttgt
     2941 ccattcatcc aggttaaaaa tttcaaccca aaactttaat tatgaaaagc ttcatagaac
     3001 gcgttcaaat gcgatttaag agcctttacc tagaaaacat agatctcata gtgaaaaaca
     3061 caaaacagct caaaaaacgc aaatgagagc gaaataaaga ggtattttga ctttagatag
     3121 atgcatagag cgagtgtcta cgagcgaact atcaaaattt gcgcctagac cctctgaaaa
     3181 acattttttt gtcctcttta gcctaagaaa gcgtaagttt cgtgcagaaa tttgctcctg
     3241 gatcgagcgt agcgagtaaa aaagttttat gagcgaagcg aattccaagt ttcttttgct
     3301 tttttttaaa gtcacgccag tattaaccaa aaaattgccc caacgaactg agcgaaagcg
     3361 aagttcaata gagtttgagc gaagcgaaaa ccaagggcaa tttttcattc cctgggcttt
     3421 taattatttt aaagttttta aatactttta aatggcttga aagccttatg aataaagggt
     3481 tttagctctt atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt
     3541 ccacgtttac cttgcaatat gtccacgttt accttgcatt agtacacaaa taatattaac
     3601 gtgtacttat acacaataaa aaatagtggc tatgagagat ttagttgtaa aggacaatgc
     3661 cttaatcaac gcaagctata acttagactt agtagaacaa cgtttaattt tattggctat
     3721 tgttgaagca agggaaagtg ggaaagggat taatgcaaat gatccattag aagttcatgc
     3781 agatagttat atcaatcaat ttggtgtaca ccgtaataca gcttatcaag ccttaaaaga
     3841 tgcttgtaag gatttattcg cgcgtcaatt tagctatcaa gagaaaaaag ctaatgggaa
     3901 tatccgaaat gttatgagtc gttgggtatc tcaaattgct tataacgaca atgaagcaac
     3961 tgtagactta atatttgcac ctgctgttgt tcccttcata acccgactgg aagaacaatt
     4021 tactaaatat gaattacagc aagttagtag tcttagtagc gcttatgcca ttcgcttata
     4081 tgagcttcta attcagtggc gaagcactgg taaaacccca accatagaac tacaagaatt
     4141 tagaaagaag ttaggcgttc ttgataatga atatttacgg atggctcatt taaaagagcg
     4201 tgttttagag ctttcaatta aacaaataaa tgagcatacg gatataactg taaaatatga
     4261 acagcataaa agaggacgtt ctatttcagg attttctttt acctttaaac agaagaagaa
     4321 ggatagccca tcaatagaaa gagatccgaa cactttggag cttttttcaa agatgaccga
     4381 tgctcaacgg catatgtttg caaataaact ttcagaactc cctgaaatgg gtcgctattc
     4441 acaaggaaca gaaagctacc aacagtttgc tgtacgtatt gctgagatgc tacaagatcc
     4501 cgctcaattc aaagagctat acccatacct aaaaaaagtg ggatacatgc catcaaataa
     4561 aaaggacacc gtaaatggct aagttatcac taagtgaagt atctaaaaaa tttcatgtgg
     4621 atagatcaac catttacaga gctgtacgta atggacgttt atcacgctcc agtgatggac
     4681 aattcgatct agcagaggtc atacgatgct ttggagaacc tgagcaaaca tctcaaaaaa
     4741 ttgaatcatc taagcaagaa ggtgatgaat ctacaaaaaa acttattgcc catttagaaa
     4801 atgaagtcaa aaaataccaa gaacgtgaag aacggttaat gcaacaaatt gaccgtatgc
     4861 aaacactcat tgagctgaaa agtgttgcac ctgccacagc agcaccacac caagatgcta
     4921 cggcatgcga caccaagatg ccacagcatg cgacaacaca acaagacact gataacaaaa
     4981 agaataatga attaaatatt gcagaaaatg tagcagtgcc acagcaagaa actacggcat
     5041 accacaccca aacgctacag catgccacgt tgcaaagtgt ggcagtgcca caacacaaaa
     5101 aacgtggttt atttggccgt gtgctgaatg ccgtctttga taatgactga gcaataaggg
     5161 tgaaagatca tgccgaaact gaaagacatt gccctgggaa ttattgtagc tccgctgctg
     5221 atcccgatca tgctgattgc atcgtaccag gataaaaagg cgctgaagaa agagctggat
     5281 gaatgccaaa aagaaaaaga ccaggcatcc tgattattca attgagctaa tcaaaagtgt
     5341 ggaacattga tgaaataata gcgtggaacg tgaaaagtca taaaaaaagc ccgaacaggg
     5401 atgctcgggc tataaactag aaactacagc gttgaattat aaggataatt atacagcatt
     5461 tcgtataagg cgtattatgt taattttaga aattattaaa tgattccaag attttctagc
     5521 gacttataag taatttcatt acgaatagaa ccagacattc cttctttcat ttctaagttg
     5581 agcgaaaagg ggattttttt tccattagct tgctccaccc aaccagtcaa ccaacctacc
     5641 tgtggagtaa catccattcc ccatccactt tttgcataaa tcttactacc atttacttct
     5701 ttaattagaa gcattttttt aacttcttct tgagtttcta atttaaaagg taatcggtta
     5761 tgtgcaaggt catcggcaaa attaacttct tgtactggtg taattttaag ggggccaact
     5821 aaccaaaaat tatcgacctg tgttccaata tttgtatttc caaaattaac ccgctttact
     5881 tctttctgca ttagctctag gccagtccgt cttgcaagct cttgatatac tggaactgct
     5941 gacaatgcca ttgcctcacc taaagtcata tctttctccc acataggata agttcttttt
     6001 ttaccatccc atttgaaaat ctcatttgtt gttgctttat gattttctag cccgattaaa
     6061 gcatttagca tcttaaatgt tgatgcaggg acatattctt tatttgctcg tgcaagagca
     6121 ttaccatagg tgctaagatt tttaccctct ttaataataa ttacaccctg tgtttgagct
     6181 tcatcaaaat agcttttaat agctttttca tgttgctgag aagaaatatg aaaattatct
     6241 tcagatttag ttttaataga tgaacatgca ctgagagaaa ctagaataga aatgctgaat
     6301 ataggaagta taaatttttt cattacaaat tcatgttagg ggaaattttg gggcttagag
     6361 tataatattt ttattaaaaa agttaaaact cttttaattt aacataatgg acgttatgcg
     6421 aagtacactt gtatattaat ataaatatca actacttaat aaaaatagaa aaatcccaaa
     6481 atgcccaaaa agccgacttt gaaacaagtc ggctttttta tgagcagttt tgatacttct
     6541 tgccaataat tttgactaaa aattacacga atctggttga gtgatacaat tttgtgatac
     6601 attattgtat atacaaattt gaatgttttt tatggaacag tattttgaat gggatgaggc
     6661 taagaatcga aagaatcaga aaaagcatga tatctctttc gaaactgcaa gccttgtttt
     6721 tgaagatcca ttacggatct caatccaaga cagacatacc aatggtgaag aacgttggca
     6781 aaccattgga cgagttaaag gcgtactaat gttattggtg gctcacacca tttttgatga
     6841 agatgactgt gaaatcatcc gaatcattag tgcaaggcaa gtcactaaag cggagcgaaa
     6901 caaatatgag catggttaga tactcacaca aagagctgaa tgagaaattt ggtgagaagc
     6961 aagatgctga aattcaacgc ttgcttgcta aaggcacggt tcctgatgat cagctagatc
     7021 tatcagatat tcctgaaatt acagactgga gcaatgctgt acgccaaaat caattttacc
     7081 gcccagtaaa gcaacaaact tcaattcggt tagatgctga tgtacttgct tggttcaaag
     7141 cacaaggcaa aggttatcaa acacgtatga atgaaatttt acgagatgct atgctaaaag
     7201 aattaaaaaa tcatcaataa gaatgggagc ttaggctccc atttttgact tcatttttag
     7261 gggggattac cattcaaaaa tttaaatagt agattttata aatttttaag tgttatatta
     7321 taatataaca ttaaatttgg ttgttttatg ctaaataaaa gtaaactatt tttagcttta
     7381 attactttag gggcctcgaa aattttattg gcagctgaag ggcctgttac taccttaaat
     7441 acaatagtat taactgcaca atctgatgag ttagggtctg agctgttagg caaaagtcta
     7501 aatgttagta atcagtttat tgatactagt aaacttaaac agcgttcgac aactctaggg
     7561 gatgccttgg gtacggaatt aggaattcat tctaatcaat atggaggtgg agcatctact
     7621 cctattattc gtgggcagga aggaaaacgt attaaagttt tacagaataa tgctgacgtg
     7681 ttggatatgt ctaatatgtc cccagatcac gctgtcacag ttgaaccaag tttagctaag
     7741 tctattgaaa ttatacgtgg tgcaagtacc cttttgtata gttcaaatag tgcggcaggg
     7801 gtagtcaatg tcattgacta taaaattcca acccaaatgc ctcaagacgg attagagggt
     7861 aatactacat tacgttttaa tactggcagt aatgagaagt tgaccacagc aggtgtaaca
     7921 gttggcttaa gcccacatgt agctttacgg gcagaagggt tatatcgtaa tgctggtaac
     7981 tataaaactc ctcattacca gtctagctct tataattctt tagaagatct tgaaaaccaa
     8041 aacagtattt ataaaaattt aaaatattta ccagaaagct gggcagagtc gagagtaggt
     8101 acgcttggat tatcctggat agacgataat acttatttag gagtttctta tacacatcgt
     8161 catgatgaat atggtttacc agcccatagc catttatatg aaggttgtgg tgcctctgca
     8221 atcggtatcg attcacgaat tagtggatta aaaaattatc ttttatatta tcctcaattg
     8281 atggatgagc aagatattaa ttacataaat ccacgtcctg attgtcatca gcataatcat
     8341 attcatgaaa ctaatttctc tcataatgct ccttatattg atttaaatac tcgacgttat
     8401 gatgtgcgtg gagaattcac tcaaccattt accggtattg acaaaattcg tacctcattg
     8461 agttatatag attattttca caatgaactc gagggagata agattactaa cttttttaaa
     8521 aatacaggaa aagtaggccg catagagtta tcacatcagc cattagggga attaaccggt
     8581 atcttggggc tacaatattt ggaacaggat aatagtgctt tatcaccagt ccattctcaa
     8641 gaaggccata ccacctatct agataatcag cagctattaa atcgaaatgt cactaaaaac
     8701 tttagtgtat ttggcctaga aaaatataac tggaacgact ttactttcga actaggtgct
     8761 cgtatagaaa agcagaaagt tagtatggat tatgatatag aaaaaattaa agattcaatg
     8821 aaaccttggc ctaataaata taacagtcct tatgtagaaa aaaataataa aatcagggct
     8881 caaaatttaa aatctatttt agaagctgtt caacctaaca aagaaacagc tttttcttat
     8941 gcaggtacag tccactggag atttgctcca
//
