LOCUS       NZ_CP064176            22246 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 19110124 plasmid p19110124-4, complete
            sequence.
ACCESSION   NZ_CP064176
VERSION     NZ_CP064176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16659219
            Assembly: GCF_015353095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 22246)
  AUTHORS   Yao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2020) College of Animal Science and Veterinary
            Medicine, Henan Agricultural University, No.15, Longzihu University,
            Zhengzhou, Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 06:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,597
            CDSs (total)                      :: 5,475
            Genes (coding)                    :: 5,349
            CDSs (with protein)               :: 5,349
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 47 of 126
            Pseudo Genes (incomplete)         :: 80 of 126
            Pseudo Genes (internal stop)      :: 15 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064176.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22246
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="19110124"
                     /isolation_source="Anal swab"
                     /host="swine"
                     /db_xref="taxon:573"
                     /plasmid="p19110124-4"
                     /geo_loc_name="China:henan"
                     /collection_date="2019-06"
     gene            join(21697..22246,1..935)
                     /gene="ltrA"
                     /locus_tag="ISU39_RS27005"
                     /old_locus_tag="ISU39_27005"
     CDS             join(21697..22246,1..935)
                     /gene="ltrA"
                     /locus_tag="ISU39_RS27005"
                     /old_locus_tag="ISU39_27005"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004152765.1"
                     /translation="MYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRTQ
                     SRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMDY
                     AGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKILN
                     AVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIRF
                     TEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWAH
                     QWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEFG
                     RFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLRR
                     RWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTTW
                     TKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            1087..1784
                     /locus_tag="ISU39_RS27010"
                     /old_locus_tag="ISU39_27010"
     CDS             join(1087..1336,1336..1784)
                     /locus_tag="ISU39_RS27010"
                     /old_locus_tag="ISU39_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X3 family transposase"
                     /protein_id="WP_223229472.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYGRMCRTVVARVFGERTLATLERLPGLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNQNLRQHLARLGRKSLSFSKSVALHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X3 family transposase"
     gene            <1794..2102
                     /locus_tag="ISU39_RS27015"
                     /old_locus_tag="ISU39_27015"
                     /pseudo=""
     CDS             <1794..2102
                     /locus_tag="ISU39_RS27015"
                     /old_locus_tag="ISU39_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2474..3178)
                     /locus_tag="ISU39_RS27020"
                     /old_locus_tag="ISU39_27020"
     CDS             complement(2474..3178)
                     /locus_tag="ISU39_RS27020"
                     /old_locus_tag="ISU39_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            3292..4068
                     /gene="aac(3)-IVa"
                     /locus_tag="ISU39_RS27025"
                     /old_locus_tag="ISU39_27025"
     ARG             3293..4065
                     /name="aac(3)-IVa"
                     /gene="aac(3)-IVa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IVa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4297..5322
                     /gene="aph(4)-Ia"
                     /locus_tag="ISU39_RS27030"
                     /old_locus_tag="ISU39_27030"
     ARG             4298..5319
                     /name="aph(4)-Ia"
                     /gene="aph(4)-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5397..>5636
                     /locus_tag="ISU39_RS27035"
                     /old_locus_tag="ISU39_27035"
                     /pseudo=""
     CDS             5397..>5636
                     /locus_tag="ISU39_RS27035"
                     /old_locus_tag="ISU39_27035"
                     /inference="COORDINATES: protein motif:HMM:NF014571.3"
                     /GO_function="GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /name="AAC(3) family N-acetyltransferase"
     gene            complement(5744..6496)
                     /locus_tag="ISU39_RS27040"
                     /old_locus_tag="ISU39_27040"
     CDS             complement(5744..6496)
                     /locus_tag="ISU39_RS27040"
                     /old_locus_tag="ISU39_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_000602738.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRDL
                     EEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRYLY
                     RAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKTSVD
                     DGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLWLRKG
                     FGFSGGWTVNDQNDLLARLFGLQKVNKA"
                     /name="IS6 family transposase"
     gene            <6558..8081
                     /locus_tag="ISU39_RS27045"
                     /old_locus_tag="ISU39_27045"
                     /pseudo=""
     CDS             <6558..8081
                     /locus_tag="ISU39_RS27045"
                     /old_locus_tag="ISU39_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            <8115..8186
                     /locus_tag="ISU39_RS28130"
                     /pseudo=""
     CDS             <8115..8186
                     /locus_tag="ISU39_RS28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(<8244..8327)
                     /locus_tag="ISU39_RS27050"
                     /old_locus_tag="ISU39_27050"
                     /pseudo=""
     CDS             complement(<8244..8327)
                     /locus_tag="ISU39_RS27050"
                     /old_locus_tag="ISU39_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /name="SOS response-associated peptidase"
     gene            complement(8968..10044)
                     /locus_tag="ISU39_RS27060"
                     /old_locus_tag="ISU39_27060"
     CDS             complement(8968..10044)
                     /locus_tag="ISU39_RS27060"
                     /old_locus_tag="ISU39_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000227969.1"
                     /translation="MVDKVTEAAVVGGVDTHKDLHVAAVVDQNNKVLGTQYFSTTRQGY
                     RQMLAWMTSFGTLKRIGVECTGTYGSGLLRYFQNAGLEVLEVTAPDRMERRKRGKSDTI
                     DAECAAHAAFSGIRTVTPKTRDGMIESLRVLKTCRKTAISARRVALQIIHSNIISAPDE
                     LREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADLDVMI
                     AAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVSSGKTN
                     RYRLNRGGDRAANSALHIIAIGRLRTDAKTKEYVARRVAEGHTKMEAIRCLKRYISREV
                     YTLLRNQNRRINSIPITA"
                     /name="IS110 family transposase"
     gene            complement(<10326..11182)
                     /locus_tag="ISU39_RS27065"
                     /old_locus_tag="ISU39_27065"
                     /pseudo=""
     CDS             complement(<10326..11182)
                     /locus_tag="ISU39_RS27065"
                     /old_locus_tag="ISU39_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611389.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc15 family transposase"
                     /name="IS3-like element ISEc15 family transposase"
     gene            complement(11386..11802)
                     /locus_tag="ISU39_RS27070"
                     /old_locus_tag="ISU39_27070"
     CDS             complement(11386..11802)
                     /locus_tag="ISU39_RS27070"
                     /old_locus_tag="ISU39_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_000754566.1"
                     /translation="MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYA
                     EMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRAAVDATTDIRVALRLAGTPIGPNDT
                     AIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(11799..12029)
                     /gene="vapB"
                     /locus_tag="ISU39_RS27075"
                     /old_locus_tag="ISU39_27075"
     CDS             complement(11799..12029)
                     /gene="vapB"
                     /locus_tag="ISU39_RS27075"
                     /old_locus_tag="ISU39_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="WP_001261276.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRP
                     TWGSFAQLDRADPDFMAEREDVVSDEGRFEP"
                     /name="vapB"
     gene            12603..12953
                     /locus_tag="ISU39_RS27080"
                     /old_locus_tag="ISU39_27080"
     CDS             12603..12953
                     /locus_tag="ISU39_RS27080"
                     /old_locus_tag="ISU39_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000493378.1"
                     /translation="MFTAFNERNDFSYAFEKIRNAISAPGENNLYAATELGLGILLRKY
                     EQFRQELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEHKR
                     FVKLAEELAAGR"
                     /name="hypothetical protein"
     gene            13004..13747
                     /locus_tag="ISU39_RS27085"
                     /old_locus_tag="ISU39_27085"
     CDS             13004..13747
                     /locus_tag="ISU39_RS27085"
                     /old_locus_tag="ISU39_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000129823.1"
                     /translation="MTDIQAATESIVAATSHPVSVQLINQGAGIWGNVATGLITGLLTG
                     GITLTGIWLTHYFTLKRERQASEDKMKKELHYIATELVFMLERYAEGCFRVVTDDGQDD
                     DAPQPERKAVTNYPELNLIDVSGDWRTLEPRLMYRIRELPVLQDEAHRAIAYAAEYSDP
                     PWHKDYFRERQYQFTRLGINAVILAVRLRKATGMPETRLTGHDEWSAVSVFRKVWRRER
                     ALRAAEATRNREWNQLVIPDGMSNQ"
                     /name="hypothetical protein"
     gene            13744..14520
                     /locus_tag="ISU39_RS27090"
                     /old_locus_tag="ISU39_27090"
     CDS             13744..14520
                     /locus_tag="ISU39_RS27090"
                     /old_locus_tag="ISU39_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_000015958.1"
                     /translation="MSGLLSYSHPQQPAQMPVAIDYPAALALRQMALVQDELPKYLLAP
                     EVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLVPPYPFVQLATLKQRTEK
                     AARTAGRAPAGSQAHRLVPLSDHHYVSQLQMMVATLKIPLERRNKRTGRTEKARIWDIT
                     DRTVRTWIGEAVEAAAVDGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSIS
                     STEVYTKVFALDVAARHRVQFQMPEADAVAMLKGNI"
                     /name="site-specific integrase"
     gene            complement(14578..14835)
                     /locus_tag="ISU39_RS27095"
                     /old_locus_tag="ISU39_27095"
     CDS             complement(14578..14835)
                     /locus_tag="ISU39_RS27095"
                     /old_locus_tag="ISU39_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000764642.1"
                     /translation="MKLETIQIDNTVRSGLNSFTDGVIIRDTKVLITLNLPDGQKTTIE
                     GEWRHMSDANRLSERITMLQHAIYEQYEEYRADIPVSWSR"
                     /name="hypothetical protein"
     gene            complement(14964..15068)
                     /locus_tag="ISU39_RS27100"
                     /old_locus_tag="ISU39_27100"
     CDS             complement(14964..15068)
                     /locus_tag="ISU39_RS27100"
                     /old_locus_tag="ISU39_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032409716.1"
                     /translation="MRSFTIMSLLETFLTASQGATILFLAMLIYWQKR"
                     /name="hypothetical protein"
     gene            15598..16464
                     /locus_tag="ISU39_RS27105"
                     /old_locus_tag="ISU39_27105"
     CDS             15598..16464
                     /locus_tag="ISU39_RS27105"
                     /old_locus_tag="ISU39_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118283.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004118283.1"
                     /translation="MGKRSSSVLNYPLVDKVQIFPVDKYRDSLQFLFSLSLWVGKNLPI
                     GIEMDTQALLPATKTFKKRASIKQSNELTEAAYYLPLQAKRVLWLCLMQAYFNDSQEDD
                     SDVLPLFKISVSDYVKYFNVATSVASRDVKAGVNALGESTVTFYPKEGEFEEVKRPWLA
                     EAGMKRGRGSWQIEFNYKVMPFLVGLTSQFTTYSLYDCGQLNSVRVIRLYESLCQFRST
                     GVWITTHDWLCERFMLPASQKNNIAEMKRTFLEPALKKINEKTPLKVTYTTEEDGRLLF
                     NFLDKKQ"
                     /name="replication initiation protein"
     gene            complement(16641..16910)
                     /locus_tag="ISU39_RS27110"
                     /old_locus_tag="ISU39_27110"
     CDS             complement(16641..16910)
                     /locus_tag="ISU39_RS27110"
                     /old_locus_tag="ISU39_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000339857.1"
                     /translation="MDAPDYGKQVNFADGPDYGKQVNFTGGRECGEQVNFADGPDYGKQ
                     VNFAGGRDCGKQVNFADGPDCGKQVNFAGGRECGEQVNFADGPD"
                     /name="hypothetical protein"
     gene            17325..18530
                     /locus_tag="ISU39_RS27115"
                     /old_locus_tag="ISU39_27115"
     CDS             17325..18530
                     /locus_tag="ISU39_RS27115"
                     /old_locus_tag="ISU39_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000368714.1"
                     /translation="MDNIEQLRKVATRAGKLLTSLSESIRQQKEELKLTEFYQEYSKAA
                     LYKLPKLSKGSVEYAVSEMEAGGYIFKKKPSGNTMKYAMTIQNVIDLYNHRKVPKYRDR
                     FDKAFTIFVCNLKGGGSKTVSTASLSHAFRAHPQLLFEDLRILAIDFDPQASLTMFLSH
                     ENSVGLVENTAAQAMLQNVSREELLSDFIVPSIIPGVDVIPASIDDAFLAEGWKGLCEE
                     HLPGKNIHAVLKENIIDKLQHDYDFIFLDSGPHLDAFLKNCIGAADLMLTPLPPATVDF
                     HSSLKFVASLPALIDSIEMDGHTCNLIGNVGFMSKILNKSDHKICHSQAKEVFGADMLD
                     MVLPRLDGFERCGETFDTVISANPATYDGSTEALKSAKSAAEDFAKAVFDRIEFIRTNG
                     GM"
                     /name="AAA family ATPase"
     gene            18530..19504
                     /locus_tag="ISU39_RS27120"
                     /old_locus_tag="ISU39_27120"
     CDS             18530..19504
                     /locus_tag="ISU39_RS27120"
                     /old_locus_tag="ISU39_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="WP_000064119.1"
                     /translation="MSNEKRKTIGRQLNTQASIAEMTDTGRSQVFTLKTGRKVTFRFVR
                     VPASEVESKTFVNQENNGRDQLALTRESLKSIIQTIKFQQFFPCIGIKQSERIEILDGS
                     RRRASAIFVRTGLDVMVTEEHLSADEARQLAKDIQTAKEHNLREIGLRLIALKESGFNQ
                     KEIAELEGLSQAKVTRALQAAAVPQDLISLFPVQSELSFSDYKLLLEVNEKLSEKGLTP
                     EELIQSVSAQLDAILSEGERPEDEQKASILKLISQASQALIDPSPKEKSVVSPLWSFEE
                     KDKFARKRVKGRTLTYEFSRMSKVIQDELDKAINEVLDRNLSQ"
                     /name="ParB family protein"
     gene            complement(19586..20857)
                     /locus_tag="ISU39_RS27125"
                     /old_locus_tag="ISU39_27125"
     CDS             complement(19586..20857)
                     /locus_tag="ISU39_RS27125"
                     /old_locus_tag="ISU39_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_001754953.1"
                     /translation="MFALADVNSFYASCERVFRPDLKGKPIVVLSNNDGNVIARSAEAK
                     PWIKMETPWFQIKNERYPEKIYAFSSNYELYASMLARVMNSLEELAPRVEQYSIDEMFL
                     DLTGVEHCMELEDFGRQLRQHVYDCTHLTIGVGAGPTKTLAKSAQWASKEWKQFRGVLA
                     LTRGNPQRTRKLLSLQPVEEIWGVGNRIARKLNVLGIKTALDLALTNPAFIRKNFSVVL
                     ERTVRELNGESCLSLEEAPPTKQQIVCSRSFGVKITEYESLRQAICQHAERASEKLRKE
                     HQYCRHISVFIKTSPFAIKEPYYGNVATEKLLTPTQDTRDIIAAATTALERIWRDGHRY
                     AKAGVMLNDFTGSGVSQLQLFDERPPRPYSAELMKVLDGINNSGLGKVWFAGRGIAPEW
                     QMKREMLSPAYTTRWSDIPSAKLA"
                     /name="Y-family DNA polymerase"
     gene            complement(20857..21288)
                     /gene="umuD"
                     /locus_tag="ISU39_RS27130"
                     /old_locus_tag="ISU39_27130"
     CDS             complement(20857..21288)
                     /gene="umuD"
                     /locus_tag="ISU39_RS27130"
                     /old_locus_tag="ISU39_27130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229772.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_000776034.1"
                     /translation="MKLQLFSTGKELPPQAHPLFADLASCGFPSPAADYVESDLDLHDY
                     CIRHPSATYYLRASGDSMADGSLYNGDLLVVDSAEKPRHGDIVVASMQGEFTVKRLLLT
                     PRLTLQPMNASWSPIYPDPDELDIFGVVTHIIHRPREMY"
                     /name="umuD"
     Typing          15599..16493
                     /name="IncR"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.442"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgatgcact ggacgcactg gccacagggc tggtacgcac taacgtaaac tgggtactgg
       61 atgccgacat cagtcagttc ttcgacaggg tgagccacga atggctgatc aggttcacag
      121 agcatcggat cggcgaccgg agggtaatca ggctcatacg taagtggctc acagccggga
      181 cgtcggagga gggtcaatgg cgagcaacgg aggaaggcac cccacagggt gcggtcatct
      241 caccgctgct ggcaaacata tacctccact acgtcttcga tctgtgggcg catcagtggc
      301 gacgtcgcta tgccacaggc aatgtggtaa tggtcagata cgccgatgac atcgtcatcg
      361 ggttcgacaa acgatacgat gcccggcgct tccgtatagc catgcagcgc agactgaggg
      421 agttcggact cacggttcac ccggagaaaa cccgtctgat ggagttcggc cgcttcgctg
      481 ccgaaaaccg tgccatcagg ggaaaaggca aaccagaaac gttcaacttc ctcgggttca
      541 cgcacatcag cgggaaagat cgcaacggca ggttcatgct gatacgaaag acccgccggg
      601 atcggatgac ggcaactctg aaagccatca aagacggtct gcgaaggcgc tggcattact
      661 caatccccga acagggaaaa tggctcagga gagtggttca gggatacctg aactatcact
      721 cggtaccggg caacttcccc accatgcaga agttcaggac acacgtaaca aacctctggc
      781 gccgggcgct caggcgcagg agccagaagg atgatacgac ctggacgaaa gcaaacaaac
      841 tggcagccgc atggctacca agggttcggg ttcttcatcc atggcctgtg gagcggttca
      901 ccgccagaca cccgaggcag gagcccggtg cgtaaatcgc gcacgccggg atctgtgcgg
      961 ggggtatccg gtaacgggta tccctaccgc gacatattta acagcgtgac gataatccag
     1021 atcgcctgcc ggataatggt gccaacttac tgatttagtg tacgatggtg tctttgaggt
     1081 gctccagtgg cttccatctc catcagttgt ccctcctgct cagctactga aggcgtggtg
     1141 cgtaacggta aaagtactgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
     1201 tggcagctac agttcactta caccgcctct cagcccggta cgcaccagaa aatcattgat
     1261 atggccatga atggcgttgg atgccgggca accgcccgca ttatgggcgt tggcctcaac
     1321 acgattttac gtcacttaaa aaactcagac cgcagtcggt aacctcacgc atacaaccgg
     1381 gcagtgacgt cattgtctgc gcggaaatgg acgaacagtg gggctacgtc ggggctaaat
     1441 cacgtcagcg ctggttattt tacgcctatg gcaggatgtg caggacggtg gtggcacgcg
     1501 tctttggtga gcgtactctg gccacgctgg agcgtcttcc gggcctgctg tcggcctttg
     1561 aggtcgtggt atggatgacg gatggctggc cgctttatga atcccgcctg aagggagaac
     1621 tgcacgttat cagcaagcga tatacgcagc gaattgagcg gcataaccag aatctgaggc
     1681 agcatctggc aaggctgggc aggaagtcac tgtcgttctc aaaatcggtg gcgctgcatg
     1741 acaaagtcat cgggcattat ctgaacataa aacactatca gtaagttgga gtcattacca
     1801 accagttcac cacctactcg ctccgcgatt gtggcagtct tcgaaatccc cggacgatcc
     1861 gcctttatga aagtcttgct caattcaaat cttcaggctt atgggttact actcatgctt
     1921 ggttaaatga ccgtttcgtc ttaccggaat cccagcagaa gaacttggca gagttgaaac
     1981 gatctttcct tgatcctgca ctcaagcaga taaatgagaa aacaccttta cttgctaagt
     2041 atagtattga tgattcagga aaatttctgt tctcaataat tgataagcaa aatcccgtct
     2101 gacataaatc agcacacatg agcctgtcat ttgacaaatt tttgtcatga agatgggcgg
     2161 atttccacac agcaccggcg cccggtaagg tgggcggatt cccacacggc accggcgccc
     2221 ggcaagatgg gcggatttcc acacagcacc ggcgcccggt aaggtgggcg gatttccaca
     2281 cagcaccggc gcccggcaag gtgggcggat tcccacacgg caccggcgcc cggcaagatg
     2341 ggcggatttc cacacagcac cggcgcccgg taaggtgggc ggatttccac acagcaccgg
     2401 cgcccggcaa ggtgggcgga tggcactgtt gcaaagttag cgatgaggca gccttttgtc
     2461 ttattcaaag gccttacatt tcaaaaactc tgcttaccag gcgcatttcg cccaggggat
     2521 caccataata aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt
     2581 tgatggtggc gtaagccgtc ttcatggatt taaatcccag cgtggcgttg attatccgtt
     2641 tcagtttgcc atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa
     2701 cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg
     2761 ctttatccgt gttgatgaat cgtgggatct gccacttctt cacgttgttg aggattttac
     2821 ccagaaaccg gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc
     2881 ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacgtagg
     2941 tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag cgcagccgtt
     3001 tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga tcgacattca
     3061 ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat ttgcagtacc
     3121 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     3181 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     3241 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac
     3301 gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt
     3361 gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga
     3421 ctgatcgagg ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat gccctcgtgg
     3481 tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga
     3541 gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc
     3601 tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct
     3661 cactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc
     3721 ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc
     3781 tatggggtgc cgagacactg caccattctt caggatggca agttggtacg cgtcgattat
     3841 ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag
     3901 agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac
     3961 attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca
     4021 gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg
     4081 agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtggagcg
     4141 gatcggggat tgtctttctt cagctcgctg atgatatgct gacgctcaat gccgtttggc
     4201 ctccgactaa cgaaaatccc gcatttggac ggctgatccg attggcacgg cggacggcga
     4261 atggcggagc agacgctcgt ccgggggcaa tgagatatga aaaagcctga actcaccgcg
     4321 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     4381 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     4441 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     4501 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     4561 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     4621 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     4681 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     4741 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     4801 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     4861 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     4921 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     4981 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     5041 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     5101 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     5161 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     5221 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     5281 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac
     5341 aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc
     5401 gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg
     5461 gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc
     5521 agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat
     5581 cgccgcccgt gcctgccgat tgggtgcaga tcttgcgcaa cgagatgccg gcatacgatc
     5641 cgcaaaccac accaactcgg aacatgggtg cagtggctga attgttccgg gcgtgtcgca
     5701 aacacttttg gaggcagcta tcacccagcc aaggccggta ttttcatgct ttgttaacct
     5761 tttgcagtcc gaagaggcgc gcaagcaaat cattctggtc attgacggtc cagccgcctg
     5821 aaaagccaaa gcccttccgc agccacagca tcgcctcgaa acccgcgatg gttcgccgcg
     5881 ccgtgttaaa ggattggaaa ccgccgatct tcggcatgtt cttcttcacc cggaaatggt
     5941 cgctctcaat cccctgctgg agatgtttgg tcacataatg cacgggatcg ggatggagaa
     6001 gcccatcatc aaccgacgtt ttgatcgttg acgggaaggt gttggccccg tccgtcccaa
     6061 tcctgttcgg cgacaacaag ggttcatctt taagcatctt tcggaagaac cgcttggcag
     6121 cgtcgagatc gcgcttagcg gtcagcagga aatccaccgg attgccatgc ttatcgatgg
     6181 ctcggtacag atagcgccat ttgccgcgga tcttgacata ggtctcatca atccggaccg
     6241 agccgcaatg gggtcgacga aactgccgca gccgtttctc gatgacaggt gcataagcca
     6301 atacccagcg gttgatcgtg ctatgatcga cctcgaagcc ccgctcgcga aacatctctt
     6361 ccaagtcacg atagctgagc gggtagcgca agtaccaggc aaccgcctgt acaatcagcc
     6421 aggcctcgaa atgcctgccc ttgaaatcat ccttcgactg gcgcttcagc ttttcggcaa
     6481 tggtattcaa aatcatcggc tcgctccgca atatcaggag cggcaacttc tctccccatg
     6541 gtcaacatcg agttaacctg aagaatttgc gacaaggcca tgcgttccgc tccggagatg
     6601 tctggcttac taggtcccgg cgctatggcg atctgaaaca cgcactcgtt ccggcacaat
     6661 ccatcgcgga aggcggtcgt ctcgctgtgc cattgcggcc ggaggaatgg ctggcagacc
     6721 ggcaagctcg cctcgacatg cggttgcgcg agcttggccg tgccgctcgc gcaggcacga
     6781 tcccgggcgg gtcgattgaa aacggcgttc tgcatatcga gaaactcgaa gccgccgcgc
     6841 cgacaggcgc cgaagatctg gtgctcgatc tctacaagca gatcccgccc acgcgcatca
     6901 ccgatctcct gctggaggtg gatgcggcga ccggcttcac cgaagcgttc acccatctgc
     6961 gcacaggagc accctgcgct gaccggatcg ggctaatgaa cgttatcttg gcggaaggga
     7021 tcaacctcgg cttgcgcaaa atggcggatg cgacaaacac ccacaccttc tgggaattga
     7081 tccgcattgg acggtggcat gtcgagggcg aagcctatga ccgggcgctg gccatggtgg
     7141 tcgaggcaca ggcagcgtta cccatggccc ggttctgggg catgggcacg tcggcttcga
     7201 gcgacggaca gttcttcgtc gctacagagc aaggtgaggc catgaacctg gtcaacgcga
     7261 aatatggcaa taccccgggc ctgaaagcct atagccacgt ctccgaccaa tatgcgccgt
     7321 tcgcaaccca ggtgattcct gcaacggcaa gcgaagcgcc ttacatcctc gatggcctgc
     7381 tgatgaacga tgctggacgc catatccgcg agcagttcac cgacacgggc ggcttcaccg
     7441 atcacgtctt tgccgcatgt gccattctcg gctaccggtt cgctccgcgc atccgcgacc
     7501 tgccatccaa acggctctac gcgttcaatc cgtcggccgc cccggcgcac ctgcgagcgt
     7561 tgatcggcgg aaaggtcaac caagccatga tcgagcgcaa ttggcccgac atcctgcgca
     7621 tcgccgccac cattgctgcc gggaccgtcg cgccaagcca gattctgcgg aaactcgcct
     7681 cctatccgcg gcagaacgag ctcgcgacag ccctgcggga agtcggtcgc gtcgagcgca
     7741 ccctgttcat gatcgactgg attctggatg ccgaactcca acggcgtgcc cagatcgggc
     7801 tcaacaaagg cgaagctcat catgcgctga agcgggcaat cagcttccac cgccgcggtg
     7861 aaatccgcga ccgttccgcc gaaggccagc attaccgcat cgccggcatg aatctgctcg
     7921 ccgccatcat catcttctgg aacaccatga agctcggcga ggtcgttgca aaccagaaac
     7981 gcgatggaaa gctgctatcg cccgatctct tggcccatgt ttcgccgctc ggatgggaac
     8041 acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt ccgccccctc
     8101 ccgcaaacga ccccgctatg gccctggtac gagcgctgaa caaaatgacg aaagcaggta
     8161 tgcctgaaag cgtgcgtatt gcctgaaaac acaacccgct acgggggaga cttacccgaa
     8221 atctgattta ttcaacaaag cccgtaggca atatttcgat cggcttcatt ggcgaggaga
     8281 gaaagatact cttcccgcgt catagactga gaaaaacgtc cgcacatggt tacctccagt
     8341 ggccagactt aaagtataga taatgccgcg tcagcggcat ggaggcgcca gctgaagttg
     8401 aatggagtac tgcgggtaaa tggcgccgtc gcccggcgca gccggctgct gttttgacag
     8461 gttctgcctc acccaccccc ttccctgcaa agctataccc gctaaaaacg gctcagatgc
     8521 ctctgtacgc ggtcggagtg gtcccggcaa ggcgacagcc gcagtcgggg cgtatctccg
     8581 cgttagcggg ccgttagggc cgcttacgag cgtgtatccc agaacttacc gcgacactac
     8641 tcacagagat gacgaagagt agttacaaca cccatcatta aaagcgacac tactcccggc
     8701 cttcggccgg gggcggcggc gcttttcagt ggtggggagg ggctcctggc tggcaaaccg
     8761 gcgccggttt tatgatgaat tttcatttga atgctgtaac taacttggtt ctccggttgg
     8821 ggcccgtcgc gcagcgatgg gagctctctc tagcgcgcgc ggtattcctg caccgtctgg
     8881 gtgaaatcag ggatcgtaaa ccggaaaatc agagctaccg cgccagtgga ctgacgctgc
     8941 tgacagcaac tgacgccctt ctaagagtca agctgttatc gggatactgt tgatccgcct
     9001 gttttgattg cgcagtaacg tgtaaacttc gcgggagata tatcgcttta gacagcgtat
     9061 cgcttccatt tttgtatgtc cttcggctac acgcctggcg acatattcct ttgtttttgc
     9121 gtcagttcgt aagcgtccga tggcgatgat gtgaagtgca ctatttgcag cacgatctcc
     9181 accacgatta agtcggtaac ggttcgtttt tccagaagaa acggggaccg ggctgacacc
     9241 acacagtgcc gcaaatcctg attctgatct taaccgttga ggattgtctc cggcagtgat
     9301 cagcaactgt gaagcgcttt cgtatccgat agcattacgt ttaatcagct caggtgccaa
     9361 ctcgtcgaca attgccgcaa tcatgacatc cagatcagcg atttcgtcat gtaactcgag
     9421 atagcgtcgg gcaagggact ttaatgaaat gcgataaacg ttggttacat tgcggtattc
     9481 actggcatca ggtctccagg aacccagggt cctgatgagc tgcatgcgcg tcatatttct
     9541 gagctgttca cgtaattcat ccggggcaga gataatattt gaatggataa tctggagagc
     9601 gactctgcgg gctgatattg ctgtttttcg gcaagttttt aatacccgca gggactcaat
     9661 catgccatca cgcgttttgg gagtaactgt cctgatgcct gagaatgctg catgagcggc
     9721 acattcagca tcaattgtgt cacttttacc ccgtttgcgt cgctccatcc ggtctggagc
     9781 agtcacctca agaacttcta acccggcgtt ctgaaaataa cgaagcaaac cggaaccata
     9841 ggtacctgtg cactcaacac caattcgctt taatgtccca aacgaggtca tccatgccag
     9901 catctgccgg taaccttgtc gtgttgtgga gaaatactga gtcccaagga ctttattgtt
     9961 ctgatctacg acagcggcaa cgtgcaaatc tttatgtgta tccacgccac ccacaacggc
    10021 agcttcggta actttatcaa ccatgacaga gctcctgtga ggaaacagag ttgaatctcc
    10081 agacagataa cccggacagg acagtaacga gacaagccgt caggcccttc ttgagtcacg
    10141 cgcatcggtg aggagatgcc tcgcatgaag gcgcttccgg caaccgacgg gtccagggca
    10201 ggacacaaaa ggtcgatcgc tgtgtgagtc aggatgcggg aaggtcttca ctgcatcagt
    10261 aatcacacca atctggttaa acggcaaact aagtggtggt aaagactgac atcggcatta
    10321 ttactatcgc tgtgcacaat gacgttttct tcaggtttac gtcgccagac cgccatcagc
    10381 agcgcatcca gtgccagctc gcgcgacagt gtcggcttca tcgaccagcc caccacatta
    10441 cgggcaaaaa gatcgataac cactgccagg tacagccagc cctgccaggt gcggatgtag
    10501 gtgatgtcgg tgacccacac ctgattgggc ttcactacag taaattcacg ccgcacgtga
    10561 ttaggcgcta tcagtgacgg tcgtccccgg ctcccgcgtg gcactttata accatgtata
    10621 gcctgaatcc tgtttttcct catgattttg gcaacccggt ttctgctgca cacttcgcca
    10681 atttcgcgaa gatcgccatg aaccctgcga taaccgtaaa ctccaccact cagcgtatag
    10741 gagtcgcgga tgagttccag cagtcgctgg ttatcctttt ctccggcaga tacagggtta
    10801 tggagccaga cataaaatcc ggcacgggca accttaagga cccgacacat tgtaacgatc
    10861 gaccagattt cacggtgatc gttgataaag cgatacttca gtcgggctcc cttgcaaagt
    10921 accgcgctgc ctttttcaga atatcccgct cttcctcagt gcgtttaagc tgtgctttca
    10981 gcctgagaat ttctgttctg gcatccagta aatcctgtgc ctgctgtcca ctgttgtcag
    11041 gcttaacagc ccggagccat ttatataggc tgtgcgccga cacgccgagc cgttcagata
    11101 cttcagcaat agaatagccc cgctcggtga tctggcggac ggcctcttcc ttaaattcag
    11161 gggtaaaacg tgatgtgccc atagactcct cctatgctca aactataggg cagatttgtc
    11221 tacgggctcg gggtcactcc attaagtgat aacaggtgtc tggaaatata ggggcaaatc
    11281 cagtcgggag gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa
    11341 agcccattca tgtctgtcgt ctccgtcacg gaaattatta cgggattatt tcacccagtc
    11401 ttccagcacc aggcccggga cccgcgcaaa ctctctcaca ttgttcgtca ccaggatggc
    11461 gcccgccgcg atggcgtgcc cggcaatggc cgtgtcattc gggccgatcg gcgtcccggc
    11521 taggcgcagc gccacccgga tgtccgtcgt ggcgtccacc gcggcgcggt cccagggcag
    11581 gacggcatcg aggcgcgcgc agaacgcgtc gaccagctga atatggcgcg gcgaggcctt
    11641 cgggccggtg gcgccgaagc gcatctcggc atacgtcacg gccgagacca caatgcgatc
    11701 accgcgcagc accgcctgct ccaggcgctt cagcaccgct gccggctgct cgcgcatgat
    11761 gaacgagcag atgttggtgt cgagcatcca ggttttcttc atggctcaaa gcgtccttca
    11821 tcgctgacca catcctcacg ctccgccata aagtccggat cggccctgtc cagctgcgcg
    11881 aacgagcccc aggtcggccg gacgggacgc agaatgatgc tgtccccttc ccggacgatc
    11941 tccagctcgc tgacgccatc aaaatccagg tcccggggca gacggatggc gcggttattg
    12001 ccgtttttaa atatcgaaac tgtgcgcatg gtttttcctc atatgctgag tgtgccaggg
    12061 agtattcccg cataggtgaa gtatatgcct gcaagcccgg cttgtatagg tgttacatat
    12121 gcagatggta ggtatatgcg tatgctttgc ctatgcagat gcccggcata tggatatgtt
    12181 acacctatgt tggtttctca cttttcccgc tcagatccga aaaaaggcag ttccggatct
    12241 gattcagccc cgccggcgcg cagcggcccg gaaacacaag atgtagcggt gtttccagct
    12301 gtcagtgacc atatatgttg tgcccctccc ctgccccgtg accggccgct caccaccctg
    12361 aaagggcgct gtggttattc cgtgtgcgtg gtacacttcc ggaaactgtg gaataacata
    12421 aaagtcggat ccggccagag cggccgtcag gtaatttaat tacgttgtta tatggctgct
    12481 tattatttta gtatgtgaaa atcactctcg tgaaagtcac tgtcgcgaca gagtggcact
    12541 catctgacga tgattgaggt gtggtaaaat tgcacggcta actattcaac tgaggaaagg
    12601 acatgtttac tgcattcaat gagagaaatg atttcagtta tgcttttgaa aaaatccgta
    12661 atgcgatttc cgcgccagga gaaaataatc tttatgctgc aacagaatta ggtcttggca
    12721 ttctgctccg taaatacgag cagtttcgcc aggaactgga tgcggccggt gagctgggaa
    12781 actgggaata tgatctcgat acctacaatc actgtattgc ggtgctgcaa cgttacttca
    12841 ccggcaatcc atccggactg acagaacgtg atgcccggat ttacagccat tacctgcaga
    12901 ctgagcacaa aaggtttgtg aaactggctg aagagctggc tgccggccgc tgactacgga
    12961 cagagggcgg gatttctcag gaatataaag gggtaaatga tcaatgactg atatacaggc
    13021 cgcaactgag agtatagttg ctgcgacatc ccatccggtt tccgtacagc tgattaatca
    13081 aggggcgggg atctggggaa acgtggcaac cgggcttatc actggcctgc ttactggggg
    13141 tataacactc actggcatct ggctgacaca ttatttcacg cttaagcgtg agagacaggc
    13201 gtcagaagat aaaatgaaaa aggaactgca ttacattgcc accgagttgg tattcatgct
    13261 tgaacggtat gctgagggtt gtttccgggt tgtgacggat gacggccagg atgatgatgc
    13321 cccccagcct gagagaaaag ctgtcacgaa ttaccctgaa ctgaatctga tcgatgtctc
    13381 cggtgactgg cggactttag agccacgtct tatgtatcgc atccgtgaac tgccggtgct
    13441 ccaggatgag gcccacaggg caattgcata tgccgccgaa tactccgacc ctccttggca
    13501 taaggactat ttcagggaac ggcagtatca gttcacgcgc ttgggcatca acgctgtcat
    13561 tcttgccgtc cgtctgcgca aggcaaccgg gatgcccgaa acacgtctta caggtcatga
    13621 tgaatggtct gcagtttccg tgtttcgtaa agtctggagg cgcgaaagag ccctgcgcgc
    13681 tgctgaagct acccgcaaca gggagtggaa tcaattagtg atacctgatg gaatgtcaaa
    13741 ccaatgagcg gcctcttatc atattctcac ccgcagcagc cggcgcaaat gccggtggcc
    13801 attgactatc ccgccgcgct ggccctgcgc cagatggcgc tcgttcaaga tgaactaccg
    13861 aaatacctgc tggcaccgga agtcagcgcc ctgctccatt atgtgcccga tttgcaccgc
    13921 aagatgctgc tggcaaccct ctggaacacc ggcgcacgta tcaacgaggc gctggcgctg
    13981 acccgggggg atttttcgct ggtgccaccg tatccgttcg tgcagctggc cacgcttaag
    14041 cagcgcaccg aaaaagcggc caggacagcc ggccgagctc ccgccggtag ccaggctcat
    14101 cgtctggttc cgctttccga tcaccactac gtcagccagc tgcagatgat ggtggccacc
    14161 ctgaaaattc cgctggaaag acgcaacaag cgtaccggca gaacagagaa agcccgcatc
    14221 tgggacatca ccgaccggac agtacggacc tggattggcg aagcggttga agcggccgcc
    14281 gtcgacgggg tgacattctc ggtgccggtc accccgcata cgttccgcca ctcctacgcg
    14341 atgcatatgc tgtatgccgg cataccactg aaggttttgc agagcctgat gggccacaaa
    14401 tcgattagct cgacggaagt ctatacgaag gtgttcgcac ttgatgttgc tgcgcggcat
    14461 cgagttcagt ttcagatgcc cgaagcggat gcggtagcca tgctgaaagg gaatatttaa
    14521 gtacttagga gaatgatgct ggcgaacgtc ctgctcgccg gggaggtctg tcagacgtta
    14581 tctggaccag ctgacgggaa tatctgctct gtattcctca tactgctcat agatcgcatg
    14641 ctgaagcatc gtaatacgtt ctgacaacct gttggcgtcc gacatatgcc gccattcccc
    14701 ctcaatagtg gttttctgtc catccgggag attgagcgtg atcaatacct ttgtatcacg
    14761 aataatcacc ccgtcagtga aagaattaag accactcctt acggtattat ctatctgtat
    14821 agtttcaagt ttcatcttgt tacctcctgt tgaccggatc ccaagcatag atatctgaat
    14881 tcaaattgcc tagtgaaatg tctggatatg ccgcattttt ccccttatac agcgaagctg
    14941 gccaaaggcc agcccgcttg gatttatcgc ttttgccagt agatcagcat ggcaaggaac
    15001 aggattgttg ccccctgaga agcggtcagg aaagtttcca gcaatgacat tatcgtaaaa
    15061 ctccgcatta ttcaggtgtg tgattcaggg gccttagtcc cccttttaca ggacgtagtg
    15121 ttctgtcacg acggaagcca gggttaataa aaacgattcg aatatgtctg cagggacgat
    15181 agaaatcttc gtgcgggttg tctcgctgta gccggctctg gagagcgact cgggggagga
    15241 gtagtatctt ccggccacgg atatgatcga gccagcagtg ctcgccgcag tcgagcgaca
    15301 gggcgaagcc ccgagtgagc gaggaagcac cagggaacaa aacttataaa ttctgcttac
    15361 gctcaatgcc tgaaaaatca cctcccctcg gggttatcca cttatccacg ggcatatttt
    15421 tataaagtac ttttttattc tttttcttta ttttacatct tttagggagc ctgaaagccg
    15481 cgtacagtgc ggcctgagag ggggcctgta tgggcaactg gtaaacataa tatgggaaat
    15541 taatcacgta tcagtgggtt ttgtgtcaac aagctatggg atacgcatca acaagctatg
    15601 ggaaagaggt caagttcagt tctcaattat ccacttgtgg ataaagtgca gatttttccg
    15661 gtggataagt atcgcgatag cttgcaattt ctgttttcac tgtcattatg ggtgggtaaa
    15721 aatttaccca taggcattga aatggatact caagcactct tgccagctac taaaacgttt
    15781 aaaaaacgcg ccagtataaa gcaatctaac gaattgaccg aagctgcata ttacctgcct
    15841 cttcaggcaa aacgtgtgct gtggttatgc ctcatgcagg cctattttaa tgacagccag
    15901 gaagatgact ctgacgtatt gccgctcttc aaaatcagcg tttccgatta cgtcaaatac
    15961 ttcaatgtcg ctacgtctgt cgccagtcgt gatgtgaagg ctggggtaaa tgctctgggc
    16021 gaatcaacgg ttacctttta tccaaaagaa ggtgagtttg aagaagttaa acgtccgtgg
    16081 ctggctgaag caggaatgaa gcgaggccgg ggttcctggc agattgaatt taactacaag
    16141 gtcatgccat tcctggtcgg cttaacctcc cagttcacta cctattctct ctatgactgt
    16201 ggccagctta atagtgttcg tgtcatccgg ctttatgaaa gcctgtgtca gttccggagc
    16261 actggtgtct ggatcaccac acatgactgg ttatgtgaaa ggtttatgct ccctgcctcg
    16321 cagaaaaaca atattgctga aatgaagcgt accttcctgg aacctgctct gaagaaaatt
    16381 aatgagaaga cgccattaaa ggtaacatat accactgaag aagatggtcg cttgctgttt
    16441 aattttcttg ataaaaaaca atagtctgta atttttattt cctcccccct tagtttgttg
    16501 ctgacatatg gggggatact gaacttgctg tgtttcccat agtgcttgcc taacttgacc
    16561 tgtttcccac actcccgacc gtccgagaag ttgacctgtt tcccatagtc cgggccgtcc
    16621 gagaagctga cctgtttccc ttagtccggg ccgtccgcga agttgacctg ttccccacac
    16681 tcccggccgc ccgcgaagtt gacctgtttc ccacagtccg ggccgtctgc aaagttgacc
    16741 tgtttcccac agtcccggcc gcccgcgaag ttgacctgtt tcccataatc cgggccgtcc
    16801 gcgaagttga cctgttcccc acactcccgg ccgcccgtga agttgacctg tttcccatag
    16861 tccgggccgt ccgcgaagtt gacctgtttc ccatagtccg gagcgtccat taaagttgac
    16921 ctgtttccca cagaggatgg aaaactcttc ttgtgctgag cgtaagagct atctgccgga
    16981 acggttcctc ttcgcttcct cgccagttcg ctcgctacgc tcggttacat ggctgcggcg
    17041 agcgctgata cataaggctc agcattgcac tggtcgtaac taaaatcaga atccttctga
    17101 atgactctcc tgtcttgcaa gttgaatacc tttctcaaag cccttttgta tgagttgctg
    17161 ttgatggtca aaagtgcgtc tgcgtgttgc tgcagtgtga tttagtagca cgcccgtcga
    17221 gcttcatccc ccttctgttg agagcgaaat caaaacaata tgattttttt cttgcaacat
    17281 gacacttttt gttcaaaatg acaaaaaacg tcatgaggtg ataaatggac aatattgagc
    17341 agttacggaa agtcgctaca cgtgcaggta agcttctgac atctttgagc gaaagtatcc
    17401 gacagcaaaa agaggaattg aagcttaccg agttctatca ggaatacagc aaagccgctc
    17461 tgtataagct gcctaagctc agtaaaggca gtgttgagta tgctgtgtcc gaaatggaag
    17521 ccggtggcta catttttaaa aagaaacctt ctggtaatac gatgaaatac gcgatgacga
    17581 ttcagaatgt catcgatctg tataaccatc gtaaagtacc gaagtacagg gatcgcttcg
    17641 ataaagcctt tacgattttt gtatgtaatc ttaaaggtgg tggttccaag acagtttcta
    17701 ccgcttcatt atcacatgct tttcgagctc atcctcagct tctgttcgaa gacctgcgta
    17761 ttctggccat cgatttcgat ccgcaggcat ctctgacaat gttcctgagc catgagaatt
    17821 cagtgggctt ggttgaaaat acggctgctc aggccatgct ccagaacgtg tctcgtgaag
    17881 aattactttc cgattttata gtaccgtcta ttatccctgg tgttgatgta atccccgctt
    17941 ccattgacga tgcattcctt gctgaaggct ggaagggatt gtgtgaagaa catctgccag
    18001 ggaaaaatat tcatgcggtg cttaaagaga acattatcga caagctccag catgactacg
    18061 actttatttt ccttgatagt ggtcctcacc ttgatgcgtt cctgaaaaac tgcattgggg
    18121 cggcagacct gatgttgacc cctcttccac cggcgacggt tgattttcat tcttccctga
    18181 agtttgtggc cagcctccct gccctcatag attccatcga aatggatggg cacacctgca
    18241 atctcatcgg taatgtcggc ttcatgtcca agatcctgaa caagtctgat cacaaaattt
    18301 gccatagtca ggccaaagag gttttcggtg ccgatatgct agacatggtt ctgccaagac
    18361 tggatggttt cgaacgttgt ggggagactt ttgacacagt tatttcagca aatccagcaa
    18421 cctatgatgg aagtacagag gcactaaaaa gcgcaaaatc agcagcggaa gactttgcga
    18481 aggcagtctt tgatcgcatc gagttcattc gtactaacgg aggtatgtga tgtcgaatga
    18541 gaaaagaaaa accatcggca gacagttaaa tacccaggcg tcaattgcgg aaatgacgga
    18601 caccggtaga agccaggtat ttaccttaaa aaccggcagg aaggtaacgt tcaggtttgt
    18661 tcgggtgcct gcatctgaag ttgaaagtaa gaccttcgta aaccaggaaa acaacggaag
    18721 agatcaactt gcactgacca gggaatccct gaaatccatt atccagacga ttaagtttca
    18781 gcagttcttt ccctgtattg gtattaagca aagtgaaagg attgagatac tggatggttc
    18841 aagacggcga gcctcagcaa tttttgtccg tacaggcctt gatgtaatgg ttaccgaaga
    18901 gcatttatca gctgatgaag ctcgccaact ggctaaagat atccagacag ctaaagaaca
    18961 taatcttcgg gagattggcc tgagattgat cgctcttaaa gagtccggat ttaatcaaaa
    19021 ggaaattgct gaactggagg ggttatctca agctaaagtt accagggcgc tgcaggcggc
    19081 ggccgtaccg caagacttga tttctctgtt cccggttcag tcggagctat cgtttagtga
    19141 ctataaactt cttttagaag ttaatgaaaa actcagtgaa aagggattaa cgcctgaaga
    19201 actcattcag tctgtatcag ctcagctcga tgcaattttg agtgaaggcg agcgaccaga
    19261 agacgaacag aaagccagta tactgaagct gatttctcag gcatctcagg cattgataga
    19321 tccatctccc aaggaaaagt cagtggtttc cccactctgg tcttttgaag aaaaggacaa
    19381 gttcgcgcgt aagcgtgtga aagggcgtac gctgacttat gagtttagcc gtatgtcaaa
    19441 agtgattcag gatgaactgg acaaggctat caacgaggtc cttgacagaa atttgagtca
    19501 ataatttcgc catggcgatt tcacatctaa cttattgtaa ttaggcagtt taggctgaga
    19561 atttcaaggt gaaatcgcca aagttttacg ccagtttcgc tgaaggaata tctgaccatc
    19621 gtgtcgtata agcaggagaa agcatctccc gtttcatctg ccattccggg gcaatacccc
    19681 gccccgcaaa ccatacttta cccagccctg agttgttgat cccgtccaga accttcatca
    19741 gctcagcact gtaagggcgt ggcgggcgtt cgtcgaacag ctgcagctgc gaaacgccgg
    19801 agccggtgaa gtcattcagc ataactccgg ctttggcata ccggtgccca tccctccaga
    19861 tacgctccag agccgttgta gcggctgcaa tgatgtccct ggtgtcctga gtgggtgtaa
    19921 gaagtttttc cgtagccacg ttgccgtagt aaggctcttt aatggcgaac ggagacgttt
    19981 tgatgaaaac ggaaatatgc cggcaatact ggtgctcttt acgcagtttt tcagaggccc
    20041 gctctgcgtg ctgacagata gcctgacgga gtgactcgta ctccgtgatc ttcacgccaa
    20101 aactgcggct gcagacaatt tgctgtttcg tcggaggggc ttcctcaagc gaaaggcagc
    20161 tttccccgtt cagttcccgt accgttcgct caaggaccac agaaaaattt ttgcggatga
    20221 aggccgggtt cgtcagggcg agatcgagcg cggtcttaat gcccagtaca ttcagcttgc
    20281 gtgcaatacg gttacccacc ccccagattt cttcaaccgg ctgcagtgaa agcagtttcc
    20341 gcgttctctg cggatttccc ctggtcagag caagtacgcc acgaaactgt ttccactctt
    20401 ttgaggccca ctgggctgat tttgcgaggg tcttcgttgg tccggcgccc acgccaatgg
    20461 tcaggtgggt acagtcatac acgtgctgac gcagctgccg gccgaagtcc tcaagctcca
    20521 tacagtgctc cacgccggtc aaatcgagga acatctcatc aattgaatac tgttccactc
    20581 tgggggccag ttcctccagg ctgttcatca cgcgcgccaa catcgaggca taaagttcat
    20641 agttacttga aaacgcatat attttttccg ggtatctttc gttttttatc tgaaaccacg
    20701 gagtttccat tttgatccag ggcttcgcct cggcactgcg agcgattaca ttcccgtcgt
    20761 tgttgctcag gacgactatc ggctttcctt tcaaatccgg gcggaaaaca cgttcacagc
    20821 tggcatagaa gctgttcaca tcggccaggg caaacatcag tacatctccc tcgggcggtg
    20881 gatgatatgc gtgacgacgc caaagatatc cagctcgtcg ggatcaggat aaatcggcga
    20941 ccaggaggca ttcatgggct gcagcgtcag tcgcggcgtc agcagaagcc gtttcaccgt
    21001 aaactccccc tgcatgctgg cgacaacgat atcgccgtgc cgcggctttt cagcactgtc
    21061 cacaaccagc agatcgccat tgtacagact gccgtcggcc atgctgtctc cgctggcacg
    21121 caggtagtac gtcgcgctcg gatgccgtat gcagtaatca tggagatcca gatcggactc
    21181 gacatagtca gcagcagggc tgggaaaacc gcagctggcc agatcggcaa acaatggatg
    21241 ggcctgtggc gggagttctt tcccggtgct gaaaagttgt agtttcatct ttcctcctga
    21301 cactctaatt ttactggtta tacatacagt gttcaccggg agaccggtag agatcaaggg
    21361 gtgaaagtcc ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc
    21421 ataccgcgag gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca
    21481 cgaaatgtat attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat
    21541 agggtgcgat actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa
    21601 tacgatctct acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagac
    21661 ggcccgcacc gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac
    21721 tcgctcatag tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga
    21781 aggagcgggg ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa
    21841 agcatgtcac agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg
    21901 tttacagcat tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccctg
    21961 agcaggaaag cggccgccgg agtggatggc atcaggtgga tggactacgc cggaaacatg
    22021 aagaacaaca taacagatct gcaccggagg ctacatcagg gcagctacag ggcgcagccc
    22081 ggcaggcgtc actacatccc aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg
    22141 ctggaggaca agatcgtcca gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac
    22201 gactttatgg ggttctcata cgggttcaga cccgggcgaa gccagc
//
