LOCUS       NZ_CP064168             3306 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain Wll001 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP064168
VERSION     NZ_CP064168.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16409547
            Assembly: GCF_015353135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Wang,L.
  TITLE     The attachment process and physiological properties of Escherichia
            coli O157:H7 on quartz
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2020) College of Resources and Environment,
            Huazhong Agricultural University, Shizishan, Wuhan 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. NOV-2015
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 14:49:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,466
            Genes (coding)                    :: 5,159
            CDSs (with protein)               :: 5,159
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 307
            CDSs (without protein)            :: 307
            Pseudo Genes (ambiguous residues) :: 0 of 307
            Pseudo Genes (frameshifted)       :: 124 of 307
            Pseudo Genes (incomplete)         :: 162 of 307
            Pseudo Genes (internal stop)      :: 69 of 307
            Pseudo Genes (multiple problems)  :: 42 of 307
            Pseudo Genes (short protein)      :: 4 of 307
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064168.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="Wll001"
                     /serovar="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:83334"
                     /plasmid="unnamed1"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2015-11"
     gene            310..558
                     /locus_tag="IMX21_RS27170"
                     /old_locus_tag="IMX21_27170"
     CDS             310..558
                     /locus_tag="IMX21_RS27170"
                     /old_locus_tag="IMX21_27170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000919062.1"
                     /translation="MAGSEQESARGSFRGETPGIFIVLSGFATSGLSVDFCDARQGGGA
                     YGKTPAVLASSGALLFAHMFFPALSPDSVDNRITAFE"
                     /name="hypothetical protein"
     gene            complement(686..880)
                     /locus_tag="IMX21_RS27175"
                     /old_locus_tag="IMX21_27175"
     CDS             complement(686..880)
                     /locus_tag="IMX21_RS27175"
                     /old_locus_tag="IMX21_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041624.1"
                     /translation="MNKQQQTALNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1152..1475
                     /gene="mobA"
                     /locus_tag="IMX21_RS27180"
                     /old_locus_tag="IMX21_27180"
     CDS             1152..1475
                     /gene="mobA"
                     /locus_tag="IMX21_RS27180"
                     /old_locus_tag="IMX21_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001511757.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFVDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(1710..2090)
                     /gene="lsoB"
                     /locus_tag="IMX21_RS27185"
                     /old_locus_tag="IMX21_27185"
     CDS             complement(1710..2090)
                     /gene="lsoB"
                     /locus_tag="IMX21_RS27185"
                     /old_locus_tag="IMX21_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(2087..3127)
                     /gene="lsoA"
                     /locus_tag="IMX21_RS27190"
                     /old_locus_tag="IMX21_27190"
     CDS             complement(2087..3127)
                     /gene="lsoA"
                     /locus_tag="IMX21_RS27190"
                     /old_locus_tag="IMX21_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     Typing          918..1143
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.583"
                     /coverage="99.0"
                     /evalue="1.05e-76"
     Typing          complement(129..657)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.227"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1153..1472
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="7.369999999999999e-60"
ORIGIN
        1 ctggcttgga ggagcgcagt caccaaaaat ctgttctttc agtttagcct taacaggtgc
       61 ataacttcaa gacaaactcc tctaaatcag ttaccaatgg ctgctgccag tggcgataag
      121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      181 tgaacggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      241 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      301 atccggtaag tggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg
      361 cctggtatct ttatagtcct gtcgggtttc gccacctctg gcttgagcgt cgatttttgt
      421 gatgctcgtc aggggggcgg agcctatgga aaaacgcctg cggtgctggc ttcttccggt
      481 gctttgcttt ttgctcacat gttctttccg gctttatccc ctgattctgt ggataaccgt
      541 attaccgcct ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag
      601 tcagtgagcg aggaagcgga agagcgcctt atgtgacatt ttctccttac gctctgttgt
      661 gccgttcggc atcctgccct gagcgttata tctctgtgct attttctact tcaaagcgtg
      721 tctgtatgct gttctggagt tcttctgcga gttcgtgcag cttctcacac atggtggcct
      781 gctcgtcagc atcgagtgcg tccagttttt cgagcagcgt caggctctgg ctttttatga
      841 atcccgccat gttgagtgca gtttgctgct gcttgttcat ctttctgttt tctccgttct
      901 gtctgtcatc tgcgtcgtgt gattatatcg cgcaccactt ttcgaccgtc ttaccgccgg
      961 tattctgccg acggacattt cagtcagaca acactgtcac tgccaaaaaa cagcagtgct
     1021 ttgttggtaa ttcgaacttg cagacaggac aggatgtgca attgttatac cgcgcataca
     1081 tgcacgctat tacaattacc ctggtcaggg cttcgccccg acaccccatg tcagatacgg
     1141 agccatgttt tatgacaaaa cgaagtggaa gtaatacgcg caggcgggct atcagtcgcc
     1201 ctgttcgtct gacggcagaa gaagaccagg aaatcagaaa aagggctgct gaatgcggca
     1261 agaccgtttc tggtttttta cgggcggcag ctctcggtaa gaaagttaac tcactgactg
     1321 atgaccgggt gctgaaagaa gttatgcgac tgggggcgtt gcagaaaaaa ctctttgtcg
     1381 acggcaagcg tgtcggggac agagagtatg cggaggtgct gatcgctatt acggagtatc
     1441 accgtgccct gttatccagg cttatggcag attagcttcc cggagagaaa ctgtcgaaaa
     1501 cagacggtat gaacgccgta agcccccaaa ccgatcgcca ttcactttca tgcatagcta
     1561 tgcagtgagc tgaaagcgat cctgacgcat ttttccggtt taccccgggg aaaacatctc
     1621 tttttgcggt gtctgcgtca gaatcgcgtt cagcgcgttt tggcggtgcg cgtaatgaga
     1681 cgttatggta aatgtcttct ggcttgatat tatattggaa tgcctttttt caaagcaaat
     1741 gatgtggctt tggatagaag gtttacgttg atcttatcaa agtttttttt aaagaacgaa
     1801 gccgagagct cagataaatc attatattca tcagttttcg taactttgtt taatgtgtaa
     1861 cttgaaaact tctcgccatt aaatgacgta tagacgtaac gatctttttt tccaccgtta
     1921 ggaattatta aatcaaaaaa aacatcaccc ttgcttttct ttttcttcaa gtcggattcg
     1981 atttttgaga aaaattcgct cgggctataa atatcagtag catagacaat aaataaagtt
     2041 ttatctttat tttttattgc ttctatttga tattttttat cttttttcat aatttcaacc
     2101 tagctacgcc accatctatt aattggcaaa cggtatcgat gattgcgatt gattctaatt
     2161 tgttaattgt cttcgtgtca gctattcctg gtttcatatg aaacaaacca tgcctgttct
     2221 catgccagta agtgtagcat tcacacaaaa cttccgctat ttcaccattt atagtttctt
     2281 ggtgtatttc tctgattata tatttgggtt tgttttcagt gaagtattcg ccaaggttct
     2341 ttgatgatga tggattgcaa acatcattaa gtatttgata tataaagcct tctatggctc
     2401 ttaatgcaga gaagcagtat gttgagtaat cttccatttc gacatctatt tttttcatta
     2461 ttagcgagca tgatagctgt tttttgatat cttcatggat tttatcgatg ctttttggta
     2521 gtttgctatg caactcggac tcaatagttt ctttttttat gtcaacatta aattctttat
     2581 ttttttgttc gacaatctct ttcatgttta gtattgagca catgaaatcg ttaatcaaac
     2641 tcgcgatttg aaggtatttt ccttggaatt gaatagagcc gcgcttgtaa atttttgccc
     2701 tgaccctgtc accattgctg gtggtcataa tatattggtg tttacaatta ggatcgttat
     2761 tattatcttc tgttattgtt atcccctctt cagaaagaaa ttcaaataga tttgccctgt
     2821 catcatcact gaattttgga atggtgtatt caaagttctt tgtgtctgaa tacaaacagt
     2881 tttcttttat aatcaaggcg atttcatcaa agtaagtgtt attttgccca gacgctcttc
     2941 cgatagtggt atttccgcct gatggcatta gttttattaa gaagtctatt cctttatatg
     3001 tgccagatat gtgagtttct ctttcgtttt ttacattaga ggaatagttt gtgacgccat
     3061 tctgcgtcag agcagactca atcttgtcaa tattgatatt tagtgcttta aacgggttct
     3121 gtgccattgg gtcaatccgt tgtttttttt gaatatgtac agatcttgtt tttttgtcaa
     3181 cggaatagct gttcgttgac ttgatagacc gattgattca tcatctcata aataaagaaa
     3241 aaccaccgct accaacggtg gttttctcaa ggttcgctga gctaccaact ctttgaacca
     3301 aggtaa
//
