LOCUS       NZ_CP064013            33309 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain EC90PP1 plasmid p90PP1-IncX4-mcr-1, complete
            sequence.
ACCESSION   NZ_CP064013
VERSION     NZ_CP064013.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16604551
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Leangapichart,T. and Sunde,M.
  TITLE     Co-occurrence of mcr-1, mcr-3, and blaCTX-M genes in Escherichia
            coli from pigs and humans in Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Leangapichart,T. and Sunde,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2020) Animal Health Welfare and Food Safety,
            Norwegian Veterinary Institute, Pb. 750 Sentrum, Oslo 0106, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061175.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 04:43:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 40
            CDSs (total)                      :: 40
            Genes (coding)                    :: 39
            CDSs (with protein)               :: 39
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC90PP1"
                     /isolation_source="rectal swab"
                     /host="Pig"
                     /db_xref="taxon:562"
                     /plasmid="p90PP1-IncX4-mcr-1"
                     /geo_loc_name="Thailand"
                     /lat_lon="16.7005814 N 102.5143558 E"
                     /collection_date="2018-12"
                     /collected_by="Thongpan Leangapichart"
     gene            86..304
                     /locus_tag="ISD27_RS00005"
                     /old_locus_tag="ISD27_00005"
     CDS             86..304
                     /locus_tag="ISD27_RS00005"
                     /old_locus_tag="ISD27_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            395..745
                     /locus_tag="ISD27_RS00010"
                     /old_locus_tag="ISD27_00010"
     CDS             395..745
                     /locus_tag="ISD27_RS00010"
                     /old_locus_tag="ISD27_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            1050..1298
                     /locus_tag="ISD27_RS00015"
                     /old_locus_tag="ISD27_00015"
     CDS             1050..1298
                     /locus_tag="ISD27_RS00015"
                     /old_locus_tag="ISD27_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            1295..1642
                     /locus_tag="ISD27_RS00020"
                     /old_locus_tag="ISD27_00020"
     CDS             1295..1642
                     /locus_tag="ISD27_RS00020"
                     /old_locus_tag="ISD27_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            2003..2506
                     /gene="taxA"
                     /locus_tag="ISD27_RS00025"
                     /old_locus_tag="ISD27_00025"
     CDS             2003..2506
                     /gene="taxA"
                     /locus_tag="ISD27_RS00025"
                     /old_locus_tag="ISD27_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            2510..3739
                     /gene="taxC"
                     /locus_tag="ISD27_RS00030"
                     /old_locus_tag="ISD27_00030"
     CDS             2510..3739
                     /gene="taxC"
                     /locus_tag="ISD27_RS00030"
                     /old_locus_tag="ISD27_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            3816..4109
                     /locus_tag="ISD27_RS00035"
                     /old_locus_tag="ISD27_00035"
     CDS             3816..4109
                     /locus_tag="ISD27_RS00035"
                     /old_locus_tag="ISD27_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            4106..4321
                     /locus_tag="ISD27_RS00040"
                     /old_locus_tag="ISD27_00040"
     CDS             4106..4321
                     /locus_tag="ISD27_RS00040"
                     /old_locus_tag="ISD27_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            4677..5297
                     /locus_tag="ISD27_RS00045"
                     /old_locus_tag="ISD27_00045"
     CDS             4677..5297
                     /locus_tag="ISD27_RS00045"
                     /old_locus_tag="ISD27_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            5311..5637
                     /locus_tag="ISD27_RS00050"
                     /old_locus_tag="ISD27_00050"
     CDS             5311..5637
                     /locus_tag="ISD27_RS00050"
                     /old_locus_tag="ISD27_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            5656..8403
                     /locus_tag="ISD27_RS00055"
                     /old_locus_tag="ISD27_00055"
     CDS             5656..8403
                     /locus_tag="ISD27_RS00055"
                     /old_locus_tag="ISD27_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            8415..9131
                     /locus_tag="ISD27_RS00060"
                     /old_locus_tag="ISD27_00060"
     CDS             8415..9131
                     /locus_tag="ISD27_RS00060"
                     /old_locus_tag="ISD27_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            9143..10237
                     /locus_tag="ISD27_RS00065"
                     /old_locus_tag="ISD27_00065"
     CDS             9143..10237
                     /locus_tag="ISD27_RS00065"
                     /old_locus_tag="ISD27_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            10264..10413
                     /locus_tag="ISD27_RS00070"
                     /old_locus_tag="ISD27_00070"
     CDS             10264..10413
                     /locus_tag="ISD27_RS00070"
                     /old_locus_tag="ISD27_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            10752..11438
                     /locus_tag="ISD27_RS00075"
                     /old_locus_tag="ISD27_00075"
     CDS             10752..11438
                     /locus_tag="ISD27_RS00075"
                     /old_locus_tag="ISD27_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            11435..12343
                     /gene="virB9"
                     /locus_tag="ISD27_RS00080"
                     /old_locus_tag="ISD27_00080"
     CDS             11435..12343
                     /gene="virB9"
                     /locus_tag="ISD27_RS00080"
                     /old_locus_tag="ISD27_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            12336..13454
                     /gene="virB10"
                     /locus_tag="ISD27_RS00085"
                     /old_locus_tag="ISD27_00085"
     CDS             12336..13454
                     /gene="virB10"
                     /locus_tag="ISD27_RS00085"
                     /old_locus_tag="ISD27_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            13456..14484
                     /gene="virB11"
                     /locus_tag="ISD27_RS00090"
                     /old_locus_tag="ISD27_00090"
     CDS             13456..14484
                     /gene="virB11"
                     /locus_tag="ISD27_RS00090"
                     /old_locus_tag="ISD27_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            14471..16333
                     /gene="taxB"
                     /locus_tag="ISD27_RS00095"
                     /old_locus_tag="ISD27_00095"
     CDS             14471..16333
                     /gene="taxB"
                     /locus_tag="ISD27_RS00095"
                     /old_locus_tag="ISD27_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            16342..16764
                     /locus_tag="ISD27_RS00100"
                     /old_locus_tag="ISD27_00100"
     CDS             16342..16764
                     /locus_tag="ISD27_RS00100"
                     /old_locus_tag="ISD27_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            16748..17053
                     /locus_tag="ISD27_RS00105"
                     /old_locus_tag="ISD27_00105"
     CDS             16748..17053
                     /locus_tag="ISD27_RS00105"
                     /old_locus_tag="ISD27_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <17166..17360
                     /locus_tag="ISD27_RS00110"
                     /old_locus_tag="ISD27_00110"
                     /pseudo=""
     CDS             <17166..17360
                     /locus_tag="ISD27_RS00110"
                     /old_locus_tag="ISD27_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            17364..17936
                     /locus_tag="ISD27_RS00115"
                     /old_locus_tag="ISD27_00115"
     CDS             17364..17936
                     /locus_tag="ISD27_RS00115"
                     /old_locus_tag="ISD27_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            17941..20094
                     /locus_tag="ISD27_RS00120"
                     /old_locus_tag="ISD27_00120"
     CDS             17941..20094
                     /locus_tag="ISD27_RS00120"
                     /old_locus_tag="ISD27_00120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            20091..20297
                     /gene="hha"
                     /locus_tag="ISD27_RS00125"
                     /old_locus_tag="ISD27_00125"
     CDS             20091..20297
                     /gene="hha"
                     /locus_tag="ISD27_RS00125"
                     /old_locus_tag="ISD27_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            20315..20779
                     /locus_tag="ISD27_RS00130"
                     /old_locus_tag="ISD27_00130"
     CDS             20315..20779
                     /locus_tag="ISD27_RS00130"
                     /old_locus_tag="ISD27_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            20866..21570
                     /locus_tag="ISD27_RS00135"
                     /old_locus_tag="ISD27_00135"
     CDS             20866..21570
                     /locus_tag="ISD27_RS00135"
                     /old_locus_tag="ISD27_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            22353..22448
                     /gene="dqlB"
                     /locus_tag="ISD27_RS00140"
     CDS             22353..22448
                     /gene="dqlB"
                     /locus_tag="ISD27_RS00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            23111..23758
                     /locus_tag="ISD27_RS00145"
                     /old_locus_tag="ISD27_00140"
     CDS             23111..23758
                     /locus_tag="ISD27_RS00145"
                     /old_locus_tag="ISD27_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            23833..24102
                     /locus_tag="ISD27_RS00150"
                     /old_locus_tag="ISD27_00145"
     CDS             23833..24102
                     /locus_tag="ISD27_RS00150"
                     /old_locus_tag="ISD27_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            24263..24892
                     /locus_tag="ISD27_RS00155"
                     /old_locus_tag="ISD27_00150"
     CDS             24263..24892
                     /locus_tag="ISD27_RS00155"
                     /old_locus_tag="ISD27_00150"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            25066..26691
                     /locus_tag="ISD27_RS00160"
                     /old_locus_tag="ISD27_00155"
     CDS             25066..26691
                     /locus_tag="ISD27_RS00160"
                     /old_locus_tag="ISD27_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            26739..27485
                     /locus_tag="ISD27_RS00165"
                     /old_locus_tag="ISD27_00160"
     CDS             26739..27485
                     /locus_tag="ISD27_RS00165"
                     /old_locus_tag="ISD27_00160"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_104772353.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQINLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(27876..28403)
                     /locus_tag="ISD27_RS00170"
                     /old_locus_tag="ISD27_00165"
     CDS             complement(27876..28403)
                     /locus_tag="ISD27_RS00170"
                     /old_locus_tag="ISD27_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(28538..28942)
                     /locus_tag="ISD27_RS00175"
                     /old_locus_tag="ISD27_00170"
     CDS             complement(28538..28942)
                     /locus_tag="ISD27_RS00175"
                     /old_locus_tag="ISD27_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(28955..29821)
                     /locus_tag="ISD27_RS00180"
                     /old_locus_tag="ISD27_00175"
     CDS             complement(28955..29821)
                     /locus_tag="ISD27_RS00180"
                     /old_locus_tag="ISD27_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            31236..31751
                     /locus_tag="ISD27_RS00185"
                     /old_locus_tag="ISD27_00180"
     CDS             31236..31751
                     /locus_tag="ISD27_RS00185"
                     /old_locus_tag="ISD27_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            31856..32398
                     /locus_tag="ISD27_RS00190"
                     /old_locus_tag="ISD27_00185"
     CDS             31856..32398
                     /locus_tag="ISD27_RS00190"
                     /old_locus_tag="ISD27_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            32395..32646
                     /locus_tag="ISD27_RS00195"
                     /old_locus_tag="ISD27_00190"
     CDS             32395..32646
                     /locus_tag="ISD27_RS00195"
                     /old_locus_tag="ISD27_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            32633..32860
                     /locus_tag="ISD27_RS00200"
                     /old_locus_tag="ISD27_00195"
     CDS             32633..32860
                     /locus_tag="ISD27_RS00200"
                     /old_locus_tag="ISD27_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     ARG             25067..26688
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(28956..29827)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2511..3736
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9144..10210
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          10753..11435
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          11436..12340
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          14472..16330
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaatgaaa tgctcatctg tttttacttc aaccactaat catgttttca cattcgaacg
       61 agtaacgctt tgcactatta tccttatgca taaggatact ggacaacagt atgttgtcat
      121 atttactgat aacaataaaa ttcgtgacta taaagccgga attgttcctc agtttggcga
      181 acttaagcag agtgatgttg atttagtcct tttctacagg gacgaatatg aaaaatattt
      241 tgattcatta aaagatggcg atgaatgctt gagttttaaa gattttattg agtgtctttg
      301 ttaagcatta tgaatgcagg ggcatccctg cattttctgg aaatttcaat attgattctg
      361 aaattgtcta ttaacatcaa tgcaagggaa attcatgaaa acgttaaaac aagccgcaat
      421 gcagtttgca tctgatctgc gtaataatcg ctgttttaaa gctgcttata atgatgctct
      481 gttagagctt gatcgggaag atttaaacgc cattgtacaa tgcacatttc tttttgagcc
      541 aaaacgaaca ttagccgaac ttgataaatt aattaaaaag atagaagtag aagaagatta
      601 tgagttttgt gtatcccttg ataaaggtaa aggattacac gaagtcagaa catttaaaag
      661 tgagcgtgag gcgttggatg cttatcgcat tttgactctt tacggataca cagtaactat
      721 agaaaagaag gagggtaatg actgactatt caaggtgtga tcaggttgtt ccaccaacct
      781 gatcacgtta tcgacaatca aaactgaatg aggtttttaa atgtcaacgc ggcagatcgt
      841 acagcttgct gattcccttg tcaaaggctg ggaaaccaga attccggcaa tgaaatttag
      901 cgaatgggat caattccggt ggtggttaaa ctacttgcaa gggtatgaaa tgttttaaat
      961 aaaaaaaggg gctttctgcc cctttgtatt ttttgacaaa aatgccatgt gtaactataa
     1021 ttagttgcat atctataggg gatggattta tggggaaaac agataagcta ctggcaaagt
     1081 ttttaaacag taaaaaaacg tttgaatggg atgaactggt cgttttgttt tcctctttgg
     1141 gatatgtcaa aaaggaaatg caggggtcaa gagtgcggtt tttcaatgct gaaatcaatc
     1201 acaccatatt aatgcatcgt ccacatccgg aaagttatat caaaggtggt acgctgaaag
     1261 caattaaaca gaatctgaaa gaggctggat tattatgaaa catttaaaat ataaaggata
     1321 tttaggcacg gttgaacccg attttgaaaa taatgtcctg tatggaaaac tggcgtttat
     1381 tcgggacctg gtaacttatg aggcttctac attagctgaa ctggaacagg agtttaaaac
     1441 atctgttgat ctgtatttac agtcttgtgt ggaggatgga aaggaacctg ataccccgtt
     1501 taaaggcgtg tttaacgtca ggcttgatcc agaactgcat cgtcgggtag cagaaatggc
     1561 gatggaagaa gatttatcgt tgaatgcctt tgttaataag gctctggaaa aagaagtcag
     1621 caatcatcgc gcaggggctt aattgccctt gtttttatgc cctgtaaggg catgggaggc
     1681 gctttgcgcc ggaagccccg cagcgcagcg aggacttaaa aagcgttacg gcttcagccg
     1741 taacaattcc cagccctgcc aggcctgttt tttctttcac aaatctttca gctatacgtt
     1801 tgcctgatgg tcgaagacga aagaatcttt cagcaacgaa atctggacat atgcagcgac
     1861 gattttatag ccaggcacag tgccacatat ggtacaatca atgcaggata gacaatccga
     1921 tgtcaaatgt taacatttga atcagattgt ccaaaaacga tgtttttgcc tgtgcctgcc
     1981 gcaggctctg ttggggtgac ttatgaagat gatagcgttc aggctaagtg atgatgagtt
     2041 aaaatttgca gaacataacg ctattttaag tggatttacc agtatcaatg cttttgctaa
     2101 gcataacgtg cttaatattg aaaccaagcc tgttaacatc ccagttaata atgaaccagc
     2161 aaagctggta tcagtgcgtc tttaccctca tgaaatagaa cttgttaaac gtaatgctgc
     2221 tcttcacggc atgagcatga gcagagaaat tgctattcgt gtgcgtcagt ctttgttaaa
     2281 gagtgaagtc tgtctttacc ctgatgaagt taaggaactt aaaaaactat caactgctgt
     2341 agatcgtgtt ggtcgcaaca ttcattttat cataaaaggt gaaagattct gtacggttaa
     2401 cgatccagat ttcagaaaag acgttattga agtgattgaa ctgtgtaagc aaattgattc
     2461 aaaacttgaa acgttaacca agagtgttgt taacagattt gggtgattta tgggtgtgta
     2521 tgtagaacag gaataccgta ttaaacgcgc taaagggact gggcgtgatc cgaaatctcc
     2581 taagttatcg ggaagacata tacatgccag taaatcggct tttaatcata aagtcagaca
     2641 tggtgcggac aagaaagcat atacctggac acctacaaaa gaagtcactt ttaaaatcac
     2701 tggctcaggt aaaacagccg caggcatcaa aaatggtatt gattacatta cccgtaatgg
     2761 tgaacttgag gcttactgtt atgatggaaa aggttcagag caaacaggga aaggagaaga
     2821 ctttaatagg gagtttactt caactttatc taaaggaaat gattactcca ggacatatcg
     2881 tggagaaaac atcgatcatg tgaaaaacat ggtcttttca ccaccaccag aagctggagt
     2941 aagcagggaa gacgctctga aagctacggt agaatttctt aaagagactt atccaaacca
     3001 tgctttcgtt gccgtatacc atgatgataa agaagatcat cctcatgtac atgttaatat
     3061 taaacttcgt gatgaagaaa caggaagaag attaagactt actaaatctg aaaccaggaa
     3121 gttcagaaac ggttttcata gaaaactgaa aggtatggga tatgatgtta ccgctacctg
     3181 gaaaaaagat cccgaacgta aacgggagat tgagcgatta caggctgaaa atccgaaaag
     3241 actccggaat gtttacaagg tggtggattt tggggagact tcatatcaga ataaggcagg
     3301 ggaaaaacga acacctttcc tcacttatga aactttaaaa ggtggaaaac aggttacgat
     3361 ttggggtaaa gacctgaaaa atcatttcga gtctgaaaag ctccagccag gaacgcttat
     3421 caaagtaaaa aaactggctc ctacgcttgt acgtagtcca atgtttaatg atgatggaac
     3481 tgttgcaggc tacagggaaa cgcatcgcaa taactggcag attgaaaata ttgcccttga
     3541 gcggaacaga gaacgtcagg ttcatgagcg tgaaaccagg cagccggaag agagcgacgt
     3601 taaaaagcag ctatccagaa aacatgagca aggtcacaat atcgggtttg ctcttgaaca
     3661 cgggtttaca aaggattcag aagaacataa aaaactaaga atacagcaag agaggaactg
     3721 gaaaggcctg ggattttaat ttgattttgg cgcaggtgaa caaagcggct actttgctca
     3781 cctgctgatc acaacaacct acgagggagg ttatcatgac tgagcatgat gctatctgca
     3841 tttctggact tcatcaaatt tttagtgatg aagaacattt gagtgagcaa caaaaagaca
     3901 ttattctgat gtacgcttat ggctataccc tgaatgaaat tgctgatttc aaaggactaa
     3961 agccttcaac tgtcagaaaa tatctcgatt ctgtaagagc tgaactggga ggtgtctcgt
     4021 tggcagggat cagaacactg gtactcattc ggactaatgc ccttctcgtg tcaagtcttt
     4081 ccaggatcag tgagaggggg aatttatgaa aaaatatctg ctttttgctt tgccgttctt
     4141 cgttgttggt tgtagtgaag aagtaaaaag tgtggattgg tgggggcagc atcttacgga
     4201 agctaaacaa aagcaggcag agtgtgagaa gtctggctct gattctcaga actgtaagaa
     4261 tgttaaacaa gcattgttta ttcagtctca gaaggatgct ccagtcccta cattcgatta
     4321 actaatgttt caatcatact agagcgccca tatgggcgct tttttttgca tgatcacacg
     4381 aatcataata tgaccatttt gtcaatgtta tttgatcgta ttgttgagca ttgttatttt
     4441 gcgaatcatg ttcgcatcta aaacaaatcc aaaacacact tatagtggat tgattaaatg
     4501 aaggagaaat cagataagtc atcggaaaaa ggggtttatg tcacgataaa actcgatccc
     4561 aggttgaatg catttatcga agagaaaacg aaaacatctt tgcttagtaa gaggaagcaa
     4621 attatttact tattaatgca gagtatgcgt attgatggat atatggagcg ttagctatgg
     4681 cgtctttatt cccagccttg ttgctgtgtg cggcgtcagt tcatccggat acgataaatg
     4741 atattgcacg ggtcgagagt ggcttcaatc cttatgctgt ggctgaaatt attcctgaaa
     4801 gggaacgcgg tagctatggc aaaagtgtta tcagtcatat gcccaaaagt aaggaggaag
     4861 ctctaacagt tatcagagaa attgaaaaca gaaaaagacg ttattctgtc ggattgatgc
     4921 agattaccag cagtaatttt tctttttatt ccacgtccgc agaaaaactc cttgattcgt
     4981 gtgaaaactt gtctgttttc gaaaagatta tcgtggattg ttataaacga gggaggagcc
     5041 tggagaatgc gcttagttgt tactatacag gaaattttag taacggtaag cgaaaagaaa
     5101 aagaatttaa taatacaagt tatgttgagc gaattggtta tacagggaac gaaaaaaaat
     5161 atgttgtacc aggtacaaga agcaacgggg gagaacagcg aaaaaatcgt tcacacaatg
     5221 cgtcagttat ctggcccgaa accattctga aatctgcttt tgttgataac tcacatccaa
     5281 caaaagttat taattaacag gacttataaa atgtcattta agaaatcagt atgtaatcgt
     5341 atcagttcat ctgtcactgc tgtaatgcag cgtaaaaata aggtagttat ggcagtcgcc
     5401 ggggcaacag ttgcatcacc ggcctttgcg gatggatttt caaaagcgga aacactgctg
     5461 gaaaaggtta aaacagggct tagtggcctt tcactggtca cggtgactat tgcctgtctg
     5521 tgggtcggct acaaggttct gtttggtgga agcacgattc gtgaatgctc acctatcatc
     5581 atcggggcaa ttgttatcgc cagtgcggct gaagttgcat caatgatggt taactaataa
     5641 agcggggacg atagtatgtc tacactttat aaagcgatga cacgccctgc tatgtatgtg
     5701 ggtgttcctg tcgtccctct tactgtggtt gcaggggcgt tatttctggc gggggtttat
     5761 atcagcaagc tgatctggct ggctattcct gtcgcggttt ttgttctcag aatgatcact
     5821 aaacaggacg accacatttt taacctttac tttcttaagc tgaaaatgct cggaaactca
     5881 gtgtgtaatc gtttttttgg cgcacgagcg ttcctctccg gacaatatga agctgttgaa
     5941 attgatgagt ttgttaatgc catgaaactg aatgagcgaa tcactactgg taaatatata
     6001 ccctattcaa gccatgttga taaaaatatt gtcaaaacaa agaacggtga ttatgtagcg
     6061 acatggcaac tgatggggat taactttgag tcaatctctg cggagatgtt agaaactatt
     6121 gactcacagg ttgccactct tgttcgttca ttttccggac tgcccgtttc attttataac
     6181 cattcatgca gggcgagttt ttatgatgcc tttaccacaa aatcaggtaa taaatatgct
     6241 gatataatat cagattgtta ctatggttcg atgaagaaaa ataaattcaa ggggaacact
     6301 ctttacttta cgctgatcta cagaccagat gggcgtgtag aaaaactgga aaaacgcaaa
     6361 aaaagtatta aagaaaagaa agatgatatt aacattcatg tgaagagaat gaatgaaatg
     6421 attaatacat tctctggcgc tcttgataaa tttacctgta aacttcttgg tatgtatgag
     6481 gagaacggaa aggtattttc ttctcaactg tcattctata attatcttct gacagggaag
     6541 ttgcaaaaaa tcagagtcac tgattctccg gtgtataacg ttcttggtgg agtggatgta
     6601 ttttttaatc atgataccgg acagatatgc cgcattgatg gaaataaatt ctttcgttct
     6661 attgaaatta aagatttctg tagtgagact gcatccggtg tttttgatgt gctccagtat
     6721 tctgatgctg actatattat tacgcattca tatacatcca tgagcaaatc agaagcattg
     6781 agtaccatta aacgtgcgga aaaacagtta aagagtacag aagatgatgc ggttactcaa
     6841 ttgcaggaac tggaaaaggc aaaaaatgat attgtgtccg gtgatatatc ctttggttac
     6901 taccatttca cgctaatggt aatggcggac agtatcaggg agcttgacga atctgtaagt
     6961 aaaattacgg cagactttac tgacctgggc attattccgg cattgtcaac catgtcattg
     7021 ccagcggcat attttgccca acttccggcg gtttttcatt taaggccgcg tttatctccg
     7081 gtatcgaatg taaattttgt tgaactggca tcattccata atttctacca ggggaaacgc
     7141 gataaaaact gctggacaga agcggttgct attctcaaaa caccaagcaa acaggcgtat
     7201 tatctgaatt tacataactc agtactgttt aaagatgaaa caggtgaaaa gaatcttgct
     7261 aatactaaag ttataggaac ggcgggttca ggtaagacaa tgtttctgtc atatcttgcc
     7321 tgttcattac agaaatacaa taaccccgaa acttttgctg attctgcaaa aaataagaag
     7381 ttaacgtgtg tctttcttga taaggacaga ggggctgaat tatgtatccg aatgcttggt
     7441 ggggaatatt acaccgtaaa aagcggtgag ccgacaggat ggaatccttt tgctctggag
     7501 gcaacaaaac gtaaccgtat ttttgtcaag caactgatgg agattctgtg taccagaaat
     7561 ggtgaaagat tatcgacaag agaaagattg cttattagcg aaagtgttga tgctgtgatg
     7621 gattttcctc ctggcgaaat gcgggaatat ggcattacca gaatgcttga gcatcttatg
     7681 cagagagatg atcgggatga gcaggagaac gggattatcc ttcgtttgtc ccaatgggct
     7741 aatggtcagg ctcatggctg ggtttttgat aatgctaaag acacattcaa tattcagcat
     7801 gtcaataatt ttggcattga tggcaccgag tttcttgatg atccgatggt atgtgcacca
     7861 attactttct atctgctgta tcgcattacg cagcttctgg acggtcggcg tcttgttatc
     7921 ttcctggatg agttctggaa atggctacag gatgaagcat tcagcgactt tgtctataac
     7981 aagctgaaaa caattcgtaa gctgaacggg ctggttattc cggcaactca gtctccggat
     8041 gaaatactga agaataagat ttcacgggct gtggttgaag tatgcagcac cagtatttat
     8101 ctggctaacc ctgatgccga ttacaatgat tatgtggaag ggcttaagct gacaccagaa
     8161 gaatttaata ttgttaaaaa tcttgatcca atgtcgagac agttcctgat taaaaaaagc
     8221 agtcttaaaa agggggatgg taaatcattt tcagcacttg cgacactcga tttatcggga
     8281 cttggtggat atctgaaaat attgtctgcc agtgcagata atctggaaat ttttgaaagt
     8341 atttatcatg aaggaatgga gcctgatgac tgggttcctg aatatctcga acgggcaatc
     8401 tgacaggtga atttatgaaa cgtgtaaaaa cattgatgct gatatcatta ttgacggctt
     8461 cattttatag tagcgcagga attccggttg ctattgatgc cagtccggaa tgggctgttg
     8521 aagccgcaag atggacagaa cgactcaaac aatggtccga aactgcgcaa cattatcaga
     8581 gtcagattca ggcatataaa gaccagttag ccacggcaac aggtataagg aatattgcgg
     8641 cattcacaaa tgaactaagt aatctacaat cagaactgac aaatatttat aagcagggga
     8701 acagttatat ttctgatttt accagtaatc ccgaaggtgc attgagcagc caggctaaaa
     8761 atctgttcag caagtatgga gccttcgata tgtgtaatac gggctatgaa cgtaatgata
     8821 atctctgtaa agcacggatt gtgtcaacgg ctgccagtat tgaacaggga aatgagatca
     8881 ataagcagtt atcaagtgca atgtctcaga ttcagagttt aagtagccga attgaggctt
     8941 caaaggatat caaagaatcc caggatttgg ctaacgcatt acaggcgcag tcgttaaaaa
     9001 tgcaggcgat aaaaatgcag tatgatgtct ggaataataa aaataaagca gatcatgaga
     9061 tgttggtcac acaagaacaa gaggccttta ttaaacagca gaaagaagct gaaccattaa
     9121 cttttgatta aaaggtggat atatggctca ggggtttttc gttaaatata attctaccgt
     9181 tatggattcg gtagataaaa tcagctccag ttatcaaaca caatttgcta atgatattat
     9241 gtcgttagca acggtatcgg ttacgttata tgtactatgg aaaggatatc aaatacttgc
     9301 cagtaaaact cagacgccct tgcaggattt agtatgggat ctatcaaaat ttgcaatcat
     9361 aatcatgttt ataactaatg cggatgggta tttaactgcg gctactgatg cgttgcaagg
     9421 catgaaagat ggtttttctg gaggggtgag tgtctggcaa actttagata acttatggaa
     9481 aagtacgcaa aatcttggtg cggaaatata ctctttagat aaatcaacat atgttaaaga
     9541 tcaaggtgta gttggtcagt ttttaatatg gactggctcg cttattttaa tggctgttag
     9601 tgtggttgtt tttttaacag cagatgtaac gatgaaactc ctgattatta cagcaccgat
     9661 atttatcttt tgtctcatgt ttggttttat tcggagcatg tttaataatt ggttacaatc
     9721 gttattctcc agtattctga cagttctatt tgccagttta gtgattcgga tagcaatgga
     9781 ttttcaaggg atgatccttt cccatgctat tcgtgctgca caaacaggga atgtaaatct
     9841 tgtatctaca ggtgctatgg gctttatggc tgggtttttg ggggctttat tggttcttat
     9901 agctaaaggt tttgctgtcc agttagcagg tgcaggtgtt gaaggtgctg ttcagggcgc
     9961 agccatgatg gggctgggtg ctgctggtat ggctactggt aagtctctga tgcttggcgg
    10021 tcgtgctggt ttaggttttg gcttagggat ggccggaaga acgggcatga attctttatc
    10081 agggaaagca gggaatctga tggggcgtgg tgcgcgaaca gccgctgaat gggcgggtga
    10141 aaagggatat caggcgctgg ctccgacagg tgctttgggg atgaaggcga gaagacttgc
    10201 atccctggaa aaagcacgag cgaggaacgc cgcatgaaaa taatgaaacg ttttttttat
    10261 gtgatgtccc tgaactggat actggtgttc gttgataaac atatcacctc cggatggagc
    10321 aggaagagaa agctaataac ccagcttgtt attttaatca cgggaattac cttatttaag
    10381 gtcttaggtg gaatcagtca gagctggttg tgacatgctt cattattttg tcatgatact
    10441 ttattttgtc ctaatccttc ttttaggagt gtttacctgc ttcgcaggaa taagacttgg
    10501 tttagggctt gcggggaaat ggagaaaggg tatggatctt cgctttttgc ttcttattgg
    10561 tgggattctt tttcttcatc ttggccttta tctcttaaag gtgttcgtga acgcttaata
    10621 aggtgaattt atgcgatgtg tgattctttt gtttttcctg tttcagattt ccggatgtac
    10681 cacaaggaat aaccttcctt ctgatgtttc cggcgaactg gaacccatta accacagcca
    10741 ggtaatcagt tatgagtgaa aaaatcgaaa agaagattga gacagctaaa tccttcgaaa
    10801 gacaactcta tgcagaaaat gaacggtcta agaaaaatgc atggcgtgtt gcagtggcag
    10861 catcattact gtccttatgt cttggggctg caatttgtgt tcttgtgcca ttaaaagaaa
    10921 aagagcttgc tattgtggct gttggcgaaa aaacaggccg tacagaactg attactcagg
    10981 ttaaacagga aaagatcctg caatctgaag cacttgggcg gtattttgta aacacgtata
    11041 ttactttacg tgaaggttat aattatccgt ctcttcagta tgattatgag actgtacagc
    11101 tatatagctc gaatacagta aaggacgatt atcttcgtct gtataacagc gatatggctc
    11161 ctgataaaat ttatcataat aacggttctt acgttgcggt tgaggtcatc tccaatatta
    11221 tttctgacgc aaccgcgcca gataaactgg cctctgtgcg ctttaaaaaa acgacgagaa
    11281 acttcacgac cggacaggtt tcagtttcat actggacggc tcgtgtgaca tatcgttttg
    11341 aaccagaaaa gtcggttaaa agttccagtc gtgagcttaa tcctctcggt tttactgtaa
    11401 ccagttacca gacagatcgg gaagtcaggg gggaatgatg aagaaattat ttattgctac
    11461 atgtttattg cttcctgggc ttgtgtattc agcagcgact ccgttacctt ctggatttga
    11521 tgcaaggatg cagactgtga gttataacgg tgcgaatacg accgttatcc ggtcaaagac
    11581 aggatttctc acatcagttg tttttgatga aggtgaggct gttatcagtg caaaggctgg
    11641 ttttcctgcc ggatgggaaa tcacaaccga tgataatgtt gtttacatca atccgcgtcc
    11701 cgttgttcag gaacaggaag gcgatgaagg cgaaaaactg aaaaaggttt tccagccaac
    11761 agaaaaggaa tgggatacga atctttttgt cagaacaaca aagcggattt acagtctgga
    11821 tctaatcctt ctgtcagaag aaaaacaagc gcaacctgct tatgttgtac aattccgcta
    11881 tccatcagaa atagcgaaaa agaatgcaga agaagtaagg ctggcaaaag aaaaacagga
    11941 aaaactcaga cagaagaaac tgatttctga aagttttgaa aaagccgatg cgcctaaaaa
    12001 ctggaattat ttcatgcgag tcaatgaaaa atatgacagt cgtcgtatcg cccctgattt
    12061 tgcttatgat aatggcatat ttaccttttt aggttttaac tcagggaaag tattcccagc
    12121 tccatttgct gtaagagatg gtcaggaaca gacacttgct tttaatgtgg aaacaaaagg
    12181 caagtataaa attatggtta tccacaacgt taatgacaaa tttgttttac gctatggaaa
    12241 cagcgttgtt ggtgttgtga ataaatcttt cggaaaagta ttaaccgacc agaggaatac
    12301 atcttctcct gcggtagaac gtgttgaggt gaataatgac tgatatgaaa aaaacagatg
    12361 atttagttga tgatgcgaat cagtcaaagc tggaaagtaa acccgttcat gatatcggtg
    12421 atatcaggaa caaaaataat cgtatgcgtt ctggctttct gtttggtctg atggctgtat
    12481 tagttattgg tatttttgcg ctgaaaacat ataaaaatta ttttgcagat gaccagcaga
    12541 aagcatcaga atcaacaggc gataccagca tttctcaggt aagtaaaatc cgtactggtc
    12601 ttggtcagaa ttttgaccct gttgagaaca aagcaattat caatccaggc gctactggtt
    12661 ctggtaatac tgtttctgac ggtcataaag aagtgccaca ggaggggttc aggaaatatt
    12721 tgtcaatacc tgttgctggt cagggaggga gtcagactgg cgcgagtggc agcagcagga
    12781 catcgcagac atcagagcca caggaagaaa ggaaagaatc aaaagaaaat gtgcctggta
    12841 aatcaggaat gaaagtaact gcgattaatc tcgatcctga tctctatatt gaggaaaaca
    12901 ggcttatacc gtgtgcttta accacacgct ttgtttctga tgttgcaggt cgtatcagtt
    12961 gtgtttttac agaagatgta tggagtgcta atcaccacac aaaattactg gaacagggaa
    13021 ctaaggcttt tggtcgttac cagaccggaa cgctgaatca tggacagggc agaatgttcg
    13081 ttatgtggga gcagttgcgg acgcctgata ataaacgaat cgatatggta aatacagcag
    13141 cagctggtcc cctgggagaa gcgggcattg atggatggat cgattctcac ttctgggagc
    13201 gttttggtgg ttctcttatg ctcagtatgg ttcaggatgt agcggcggcg gcggcggata
    13261 atgcacctgg taaagatcgt aatgtggatt ataccgaaaa ctcacgacag gcaatggctg
    13321 aaatggctaa agtcgcactt gagaattcga taaatattcc tccaacaatg tataaaaacc
    13381 agggtgacat aatcactatt atggtcgggg aagacattga tttttctgat atctatgagt
    13441 taaaggtaaa atagtatgag tggcgttatt cctgtcagca ttagcaataa atcgctcgat
    13501 ttttataaaa atcaagtatt ccagtctttt cttgatattg aagggctaac agaaattgct
    13561 gtcaaccgtc ctggtgaaat ctggacggaa attaatggtg actggacatt ccataataat
    13621 gacagtgtga cctttgattt ttgtaaacgt ttttctcata cgctggcatc atttcgggga
    13681 gatgaaatcg gcgagaccaa gccattactt tcagcaacac tggaatcagg agaacgtgta
    13741 caggtcgttt ttcctcctgc atgtgagaga aatactattt caatcactat acgtaaacca
    13801 tcgacacggc agataacaca taaagaatat gtggcgaacg gtttttatga ttatgtccag
    13861 tcaggaaaaa aacacaggac atacgatgat gagcttcttg atttattcag agcgagaaat
    13921 atcgctgctt ttatggagct tgccgtagca gcagggaaaa ccattgtatt tgccggagca
    13981 acgggttccg gtaaaacaac ttatatgaag agtcttatcg atttcatacc attgaataca
    14041 cggctgatta cgattgaaga tgcagaggaa atcaaatttt ttatccataa aaattatgtg
    14101 catctttttt acccttcgga atccggcagt gattccggct ctatcataac agcagcgaaa
    14161 ttaatcaaat cctgtttgag gatgaaacct gacagaattt tactggcgga aattaaaggt
    14221 ggcgatgcct gggattttgt gaaagttgct ggttctggtc acagtggaag catgacgtcc
    14281 attcacgcgg cctcggcaaa agatgccatc attcagatgg tgacaaaatg ttatcagaac
    14341 agagaatgtc agaatcttcc ttttgatgtt cttcgtaaaa tcattatgga cagtattgat
    14401 attgttgttc atgtaggcag ggatggtgtt gtcaggcata tgagcgacat ctattacaaa
    14461 ggggctgaat gtgaaaactt ttaataagtc gcaatctgtt tttatattcg ctgttatgct
    14521 tgttgcggca tggtttgcag gttcatttat tttctttgtg ctttacttca gccagataaa
    14581 gaatctgaaa ccattgaagg ctgtgttcag atccattgat gcgtacagga tggatatttt
    14641 tacagacagc cttactttat ctgttgtgac tacggatatc agagttttag cctttgttgc
    14701 acttggagca ggatttgtcg tgtctcttat catccctgtt gcggcgttga taaaactgaa
    14761 cgagaagaaa gaaaatattt tcggtgatgc gcgatttgca acaattaatg atattcgtga
    14821 gtctaacagt ttcacgcttg acggtgacga aaaagacggg attatcgttg gtataaaaga
    14881 caagaaaatc atacgctatg tgggagcagc attcagtgct atgggagccg gaacaagggc
    14941 tggtaaaggt gctggtatag tcatcactaa tcttatgaag tactggtgga gtgttattat
    15001 tctcgatcca aaaagggagt gttttaatat caccagtctt atcaggaagg tgattttagg
    15061 tcatgaggtt tataaatttg atccctttag ttcggtaacg caccgcttta accctttgta
    15121 ttatgtcagc atgggaacga gtgaaggttt taatcagctt gaaaatcttt cgttgattat
    15181 ttacccctat aagacagatg gtgcggatgc tggtagctat cttaataaga cggcgggtgg
    15241 tgtttttaaa tcttatgctg tcgctttatg gtttatgatt aagaatgata aggctggact
    15301 aaaaacgctt gatattgagc ctgtcttctc aatgtctaaa attaatcagc tttttgagcg
    15361 tgcagaaccc gaacatcttc tttcattcat gaatgatatt cgttcagaat taagggggaa
    15421 agacaaaacg cttgctgata ttggtgtcgc tggccttaag gcattcattg agatggagga
    15481 taaaacaaaa gcacaactca aaagcaactt tcttaatgga ttatcaccgt ttgctaaccc
    15541 caatgtggca aatgctactg atggaaatga ttttgatttg cggcaggtgc ggaaaaagcg
    15601 gatgacaatt tatttctgta tttctggtga taatgccagg ttggctgaaa aaataacaaa
    15661 tattttcttt cagcttgcta tacaggtgaa tcttgaaaag atgccaacgg atgatccgga
    15721 aataaagcat gactgccttt ttctactgga tgaattcccg tcaattggtg ctgtagatta
    15781 catcaagagt aaatctggtc tcattgctgg ttacaagctg aaattgttga ttgtttacca
    15841 ggtcggctct cagcttgagg aaatctacag ttatgcaggc agtaaaacat tactggcaag
    15901 tgcaccctgc aaaattgttt attccgcttc tgatgttaaa gatgcgcggg agttatcaga
    15961 agcgatgggg acacgaacag taactatcgg ttcgaaaagc aaaagtcgta gtcgtggagg
    16021 aacatcgaga tctgaatcag aaagccttat tgagcgtcca ctggtaacaa caaatgaact
    16081 acttacactg aaatttagtg aagaaatcct ggcaatgaag ggagaaaacc cgattcgttg
    16141 tgaaaaggca ttctactatc tcaatgagta tttctttggt gactttgtga aggttgctcc
    16201 ggaacttgca aacattttcc ccaggaagaa aggaaagctg gttatgcctc cacagaaagt
    16261 atttgaaaat gaaatcgtcg caaaagggta tctggtagta aaagatgttc ctgatctcga
    16321 taaagcggca taaaggagaa catgatgaag aagttagtgt ttttattgat ttccattctt
    16381 gctggttgtt catcaccgcc agagccaaca cccgtgcagt ttgaaaaggc aaatgaagtg
    16441 atcaatccct cattgccata tgtccccgat tttcacggtg tcataaagtc agacgttagc
    16501 ggtaaaggat gggtttatga gatcacttcc ttatccggag tacaggccag aacgccgacg
    16561 ttttattacg ctctggctca tgcggacagg attgtagtga caacacatga tgctgggctg
    16621 tggtttcgaa tcagagatct gttgaagatg gaaggggcaa cagcagtcgt ggaatggcga
    16681 aacgagaaaa gttttttacc tgaacaggta aaaatagttt tcattaaagc acaaaatgag
    16741 gtgaaaaatg actggaaaaa atagagtggt tttaccagcc ttgctggcgt taggattaat
    16801 atcaacaaat gctaatgcat ctgacccttg cgcttcggta ctttgcttat atggaaaagc
    16861 agtcgggcaa ggtggaggga gtgaatgcag aagtgctgaa aaggattttt ttaatattct
    16921 gaaaaagaaa aaaggaagca ttcgctggag taaaactttt gatgcccgta aagcatttct
    16981 gaatcagtgt tcaacggctg atcctgctgc tatatcaaaa atcatgagta agtttggtcg
    17041 tgtcagaggt taattaaaac ctgataaccg cgccaacggg tatcagggta ctgcaagtat
    17101 cacatttcgc gccaacgaaa tgtgatgatt agttacaatc actgaggtta gaaatgaaag
    17161 taaccaaatc tattatagct attttattgt gcttatctgc tggtagtgca taagtggatg
    17221 taataaaaaa tgctcgtgcc tttttgaaag gaaccctcga tctgagcgtt caggaagcag
    17281 atgaagatat agaggaaagg gaagaacttt ataaaaagaa cggggcacaa cctgactatt
    17341 taagttacct ggaggattaa cacatgcaat atgcattgtt tgatggattt gaacgaaagt
    17401 ttttgctgga tgctcttgaa ttcggtgttc tgaaggactg gaaagaaaat ccggtaaaag
    17461 aacttcctga tattgatgaa tctgctcacc ccttccatat ctgttatggt ggatatttat
    17521 taaaccctgg tgtttcagat tcagatatta gcagaaaaat aaaagaccag gcaggattct
    17581 ggctggcagc tattgatgat acccgcatgg attgtcattc aatagcttat tatgatattc
    17641 acacactccc tctgatttcg tgtggtcatc aaaagatagt tccttttgcc gcgttaataa
    17701 aagctgatga atgcatcatt tcaaaaattg cttcgtattc tggttttgct gtaacagcct
    17761 ttttgagaat taaagagtgg gatattgcaa ctaatatact taaccgtgag ggaatttttg
    17821 cctttaatgg ctgtgagcgc agattcagag tagtaagtaa agataactgg caacatacag
    17881 tatcggaaga acgcgctatc cgttgtgcca aaagattaat tcaatgtaaa ggataacaaa
    17941 atgagacttt ttatcgcaga aaaacccgca gtagcaaatg atattgttaa ggcacttggt
    18001 ggcaatttta cccgccatga tggctggttt gaaagtgata acgccattgt gactaactgt
    18061 tttggtcata ttatcgaatc acaaccgccg gaaaactata atcctgaata caaagcctgg
    18121 aaggttgaaa cgcttccttt acgtctttat cccgtgaagt atcagcctgt tgaaagtgca
    18181 gcaaaacagg ttaaaacgat tctcgaactt atcagacgtg gagacgtgac tgaaattgtt
    18241 cacgctggcg atcctgatga tgagggacag ctactggttg atgaagtcct ggaatatgca
    18301 ggcaacacaa aacccgtaaa gcgcgttctg attaacgaca acacgcttcc ggcagtgaaa
    18361 aaggcactgg caaatcttaa agacaatcgt gatttcaaag gactttacct taaggcgttg
    18421 gcgcggtcag ttgccgatgc cgtctatggc ttctctatga cacgtgcgta caccattccg
    18481 gcaaaagcca gaggatatca gggcgttctg tctgtcgggc gcgtccagac tcccgttctt
    18541 ggcctgattg tgaatcgtac ccgtgctaac cagaaccata aatccagttt ttactacacc
    18601 atgaccggag tctttcagcg tggtgctgat gttatcaggg cgaactggaa gccaggggaa
    18661 tttgctccgc tgacagaccg taaattactt gataaagcgt gggcagacgg aacggctgca
    18721 tcccttgcag gaaaaccggc tacagttgaa gcagcagcaa ctgatgataa aaaaacggct
    18781 gcgccgttgc cgtttaatct ggtcagactc cagcaataca tgaacaagaa gtttaaaatg
    18841 acggcacaaa aaacgctgga tattacgcaa caactacgcg aaaaatataa agcaattacg
    18901 tataaccgct ctgactgctc atatctttca gatgaacaat tcagcgaagc gccgcaggtt
    18961 atcgatgccc tgaaatcagt ctttcctcag tcgctggata ttgattctgc acgtaaaagc
    19021 aaagcgttta acagtgcaaa ggtgactgcg catactgcga taatcccgac cgccagtgtg
    19081 cctgatgtta acgcactcag caccgacgag cgcaatgttt acctggcgat cgcacaacac
    19141 tatcttgttc agttcatgcc tgaaaaagca taccaggaag tatcggttgc cattcagtgt
    19201 ggtgatgagt cgttctatgc ccgtgccaga aaaacaactg acagcggatt tgaggcgttt
    19261 cttggcgcgg aaaccacaga cgaaggtgaa tcagaagata atgatgattc cgcttttgaa
    19321 ctgctctgta aaattcgcac aggagaaaca ctgacgacaa aagaagttat tgttaatgag
    19381 aagaaaacaa caccgccgcc gttattcacc gaagcctcct tgcttgctgc gcttgttcgt
    19441 gtcgcggatt ttgtcactga tccaacaatt aaaaaattgt tgaaggataa ggataaagac
    19501 aaaaaagatg aacatggtgg cattggtacg ccagctaccc gcgcagccat tctggaaacg
    19561 ctgaagaaaa gaaactatat cacgctggaa aaagggaaac ttattcctac tgataccgga
    19621 tatgcgctta ttgatgccct gcctggtata gcggttaatc ctgatatgac agcattatgg
    19681 tctgaaaagc aggctgccat agaaaatggc gacctgacgg ttgaacagtt tattaatgat
    19741 ctgtacggtg aactgacagg catgatttct gatgttgacc tgggcgagat gaagattgaa
    19801 cccgctgcgc cagcagggca gtttcaacgc ctggactctc cctgcccttc ctgtggtaaa
    19861 catattgtta tcaggccgaa aggttatttc tgtaccggat gtgaatttaa aatctggagt
    19921 gagttttctg gtaagaaaat cacccaggca caggccgaaa aactggttaa atcagggaaa
    19981 accgatttga ttaagggatt taaaaagaaa agtggtggaa cgtatgatac agttcttgtc
    20041 cttgaggata agaaaacagg gaagctgggt tttccggcaa gggctaagaa gtgaaaacaa
    20101 agcaggaatg gctttttcag ttaagaaaat gtacatcaag agatactctt gaaaaagtta
    20161 ttgagattaa ccgttacaaa ctgcctttat cagaatcaga ggcattttat tctgccgcag
    20221 atcatcgccg agcagaactg gtgatgaata aactttatga taaggtgcct tccggcgtct
    20281 ggaagtacgt acactaaata agaggattaa ttatatgagc gaactgacta aagaagatga
    20341 atacggcatt atcagccgta ctatgatgaa tattcgttca ttgcgtgttt ttgcccgtga
    20401 gattgatttt gagcagttgc tcgaaatgca ggaaaagctc aacgttgtta ttgaagaacg
    20461 tcgtgaagat gctgaacgtg aagcggctga acgccaggaa cttgaagcca aacgtcagca
    20521 ggccattgaa tacatcatat ctttaggtct tgatcccgaa tcactccttg ctcctgtaac
    20581 agcagataca gtaaaaacca ggcggaaagc caaaggcgga gtaagaaagg ctaaataccg
    20641 ctttaaggat gaaaatggcg agatcagaga atggtctggt aatggcaagc gtccgcttgc
    20701 tttacagaag ctgcttgatg aggggcattt catggaagat ttcctcattg agaaaactaa
    20761 gccggaacag gcagaataac ccgccaagta tacatcacac agggcactgt tgcaaatagt
    20821 cggtggtgat aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa
    20881 ggccggcatt ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc
    20941 atcagttacc gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc
    21001 acgatttacc gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac
    21061 tggcgtaacc cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat
    21121 ggccgctggg cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat
    21181 ctctcctccc gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac
    21241 gtgaagaagt ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc
    21301 gcgcttgctc tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt
    21361 aagtaccgga acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc
    21421 acgctgggat ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg
    21481 cgtgcactac gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg
    21541 cgcctggtaa gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca
    21601 tcgctaactt tgcaacagtg cctcacacag acatacacat aggtatgtct gtgtgacata
    21661 cctatgtaca gtgccagatg gcgttgctac gtgaaaaata acctacccta cccctgcttt
    21721 tttcgcagat tttacgtagc acaccgttgc tatagcattc atctaaaaag ctgaatgtcc
    21781 ttttctgtcg ctgttgcatt ttaattcact gaaaaatatt gagtaatgaa aatgccggta
    21841 acgcgtcaag ctgttggcca acgcgacagg cagtattgga agcaacaaaa aaaatgaaag
    21901 aaaaatccct caaatgaagg agaaaaaacg gaactacagc cgaacgacag gcacaaaaaa
    21961 acccgactgg taatcgggct tttttgcgtt ttccagtctt gagttggtgg ctctgaccgg
    22021 agaaggttac tctacaccta acgtttacat tttattcact atggtgctgt aaagcaacta
    22081 aatgtggtta aggaaccaca tttagtggtg taacgggtgt tttgtgtagc gtataatcag
    22141 tcagttagtc atgtagttgg cttgcaatcc tgctgttccc gtatctgcgg gtatgaacga
    22201 ttttaaagtc ctggctggat aaacaggtgg tgctgtattc ggtgctctgg caggaggagg
    22261 tcactctaca cctaacaatg cgagagagcc gttgtgacag gcagtgtatg agcaagcaac
    22321 ggtgatttcc tccagtttta ctggagaaac ggatgattaa tatgttaatc atcgttctca
    22381 gagctgtggt cgccgttgca aacgcgctga ttgcagttct ggaactgatc cgccaattca
    22441 tcgattgatg acaacggaaa cgtaactaag ggcaggaagt gaaccgcttc ctgcccttta
    22501 acaaaaaagg aggatttatg gattttatga gcattttggg gcgctgttta cttcttcctt
    22561 tccctgttgc gatgtttgtc ggttgcctgt ttcctggcat cgatgatcgc ttatccggaa
    22621 tgcttatgag tgttgctgtg ggatgtttat cgtggtatgt caccagaccg aagaaccgac
    22681 agacaaaggc cgcgtaagtg gcctttaatt gggataccct ttagttacaa cattctaata
    22741 gctcattcca tcctccgctc atccagcaaa cataacctcc ctgcccctcc ccactacaga
    22801 aagtggcgcg ccccgcccgt acgggcggaa tctaatcgct taattttgaa tgttgtaact
    22861 aaacatactc attgctgtat tgccacaagg tttcgcagca agggaggata ccgaaagtaa
    22921 cgctgtcact tttgctttca agtgaccaac caacgggtac atcacactga catacaataa
    22981 tgtatttctt catgatgtac atcattgtaa ttcattgtga catacactat agtatgtcca
    23041 tgtgacatac gcatgcgtac atcacataga aatacacatg agtaaatcac tgtgacatac
    23101 aaggggtact atgattatcg taatcggtgg tgataaaggc ggtacaggta aatcacatct
    23161 ggcaaccaac ttaactgttt gcctctcaca acagggaaag agaacggggc ttgttgaaac
    23221 agacctgaat ggctcgacca aaaaatggaa taaacgcaga gaacaggccg gattaccgcc
    23281 cgtcgcatta aatgaagcct atggcgacat cagtgccaaa ataacaaaaa tggcagatgt
    23341 agcagaaatc ctcattatag atactgccgg atatgacagc acggagttca gaactgcact
    23401 aaaagttgca gacattgtga ttgtcccgat cgacccgctt gcccaggtgg aagcagacag
    23461 tcttcagact gtgacaaaaa ttgtcaggga tgcgcaaaaa ataaatccca ggctggctgc
    23521 acatgtgctc ctgtataaat gccagccaaa tacgttcagt gaacaacaag aattaagaga
    23581 ctctctcaac agccatgatt actggcttaa accaatgaaa agcacggttt cttttctccg
    23641 ggcctttgta agagcaatga atcagggaat gggggttcac gagttaaaaa gtaatgttag
    23701 cggtgccagc caggcaaaag cacaaatcga actcttgctg aaagaactgg aactgtaaat
    23761 catagtgaca tacactaatg tacatcatca tgatatacat tagtgtgaaa caatcagatt
    23821 agaaggtaca tcatggcaaa gctggaacta ccagaactag aacaaaacca ggaagtgaac
    23881 gccacaaaat ttgttcagga cgcaggaaaa cgccccacag agaacaaaac aaaactggct
    23941 tcattccgca taccgaatga attgcttgaa ttcattgata acgaatcaaa aagactgcat
    24001 cgtcccaaga caaaaataat caaagcagca ttactggctt atcaggatct ggacgaaaac
    24061 gaactcatta atttgtggtt cagagctgac agaaatgatt aatcatagca agtggcccaa
    24121 taaactcttt atcattttct tttagatact tatctgaatg tactaacttc atcactttta
    24181 aatgtagttg tgattcattt ttagttactc ttgcaataaa atacccttgt acagttgttg
    24241 cacgggtatt taggagcact agatgagcct tatcgattta agtttatcag gattatcaga
    24301 accaggaaca aaacttattg aaaaaataag tgatgctatt ggtgtgcttt atgaacctac
    24361 aagaatcagg aaaaaagcga aggctgaagc tgaagctaaa cgcacagagc taatttctag
    24421 gttagagctt gagggaatag aaaaacgggc agttgagcgt tttctcaaac gtgaaacgaa
    24481 acgacaggaa aacattgaaa atataacaat gcaagcagca caaagtctat ctgagagtga
    24541 taatgtttct gatattgacg aagattggat agaggctttc ttcagggagt gtgaggatat
    24601 tagtgacgaa caaatgcaaa tgctttgggg aagaatttta tccgaagaag caaagtcaaa
    24661 aggttcgttt agtcgtagaa ccttaaaact attatccact ataagcaaag aagaagcgaa
    24721 ccttattact tattttggaa agtttgtttg gcaggcaaac aaactcactc caattttatt
    24781 taccgatgag aatggagata ctgaaggtat aactttcgat aaactttcgg ttttagactc
    24841 cttaggcgtt attcaacaag gcattggtta tagtttaaga tacaaattat aaatactctc
    24901 aagtgtatat tcagtatggg attgcgcaat gattgcctaa taaaatttct gaaatatttc
    24961 tgtatcgcat aattttttat atcagataaa ttgtactgga tttcttaaaa aattgcagta
    25021 tgattgccgc aattatccca ccgtttattt tttgagtagt ttctcatgat gcagcatact
    25081 tctgtgtggt accgacgctc ggtcagtccg tttgttcttg tggcgagtgt tgccgttttc
    25141 ttgaccgcga ccgccaatct tacctttttt gataaaatca gccaaaccta tcccatcgcg
    25201 gacaatctcg gctttgtgct gacgatcgct gtcgtgctct ttggcgcgat gctactgatc
    25261 accacgctgt tatcatcgta tcgctatgtg ctaaagcctg tgttgatttt gctattaatc
    25321 atgggcgcgg tgaccagtta ttttactgac acttatggca cggtctatga tacgaccatg
    25381 ctccaaaatg ccctacagac cgaccaagcc gagaccaagg atctattaaa cgcagcgttt
    25441 atcatgcgta tcattggttt gggtgtgcta ccaagtttgc ttgtggcttt tgttaaggtg
    25501 gattatccga cttggggcaa gggtttgatg cgccgattgg gcttgatcgt ggcaagtctt
    25561 gcgctgattt tactgcctgt ggtggcgttc agcagtcatt atgccagttt ctttcgcgtg
    25621 cataagccgc tgcgtagcta tgtcaatccg atcatgccaa tctactcggt gggtaagctt
    25681 gccagtattg agtataaaaa agccagtgcg ccaaaagata ccatttatca cgccaaagac
    25741 gcggtacaag caaccaagcc tgatatgcgt aagccacgcc tagtggtgtt cgtcgtcggt
    25801 gagacggcac gcgccgatca tgtcagcttc aatggctatg agcgcgatac tttcccacag
    25861 cttgccaaga tcgatggcgt gaccaatttt agcaatgtca catcgtgcgg cacatcgacg
    25921 gcgtattctg tgccgtgtat gttcagctat ctgggcgcgg atgagtatga tgtcgatacc
    25981 gccaaatacc aagaaaatgt gctggatacg ctggatcgct tgggcgtaag tatcttgtgg
    26041 cgtgataata attcggactc aaaaggcgtg atggataagc tgccaaaagc gcaatttgcc
    26101 gattataaat ccgcgaccaa caacgccatc tgcaacacca atccttataa cgaatgccgc
    26161 gatgtcggta tgctcgttgg cttagatgac tttgtcgctg ccaataacgg caaagatatg
    26221 ctgatcatgc tgcaccaaat gggcaatcac gggcctgcgt attttaagcg atatgatgaa
    26281 aagtttgcca aattcacgcc agtgtgtgaa ggtaatgagc ttgccaagtg cgaacatcag
    26341 tccttgatca atgcttatga caatgccttg cttgccaccg atgatttcat cgctcaaagt
    26401 atccagtggc tgcagacgca cagcaatgcc tatgatgtct caatgctgta tgtcagcgat
    26461 catggcgaaa gtctgggtga gaacggtgtc tatctacatg gtatgccaaa tgcctttgca
    26521 ccaaaagaac agcgcagtgt gcctgcattt ttctggacgg ataagcaaac tggcatcacg
    26581 ccaatggcaa ccgataccgt cctgacccat gacgcgatca cgccgacatt attaaagctg
    26641 tttgatgtca ccgcggacaa agtcaaagac cgcaccgcat tcatccgctg atttctccct
    26701 gtattttttc caaacccacc gcacactcca ttcgtattat gggcggtggg gtggggtttg
    26761 ttatgccgta tttatcaaat aaacgcctac ttgctgagat gagtatcgct cttgtcatgg
    26821 cgatcgttgc cacgctgacc cttgagcaca gtcagattaa tctgatggtc gctgattggt
    26881 tttatctggg tatggggcat tggatggttg ccaagcaagc ttttttgcca gatttgctac
    26941 tgtattctgg actaaaaaag ctgctgatgg cgatgctgat ctacttgctg gttgcgacca
    27001 tttgccgtgc ttatcatgag aaaaagggca atgctatcac tgccaagtgg cttgtcccag
    27061 tgacaaaatt tcgcgtgcgt gagcttgcgt atctggtgct gactttgatc ctagtgccga
    27121 cagttgtcgc gtcattgaag gcatatactc atgtggtctg ccctgtgcat ttgacgattt
    27181 ttgatggtac gctgccgtat ttgccgatgc ttgatagtat gcgtaacacc attcctgata
    27241 agtgctttcc tgcggcgcat gccagtagcg gatttgcgct gtttgccttt gcgtttgcgc
    27301 caagtttgcg ccgccgtcgt ggtgcgatca tcatcgtggt gatggcattg ggctgggcga
    27361 tgggctgcta taagatgatt attggcgatc attttttgag ccatacggtg gtgtcgatga
    27421 tgcttgcgtg ggcgatgtcg gcagggcttg cgtgggtgtt ttttaagaag ggtgaacaag
    27481 tttaatcggc tttaaaagta aaaacggtcg tatcggttat tacggaacgg ttttttcagt
    27541 tgagtttcaa agcgatgatt catcaacctg gaaactacca ataggaacgg ctttacttac
    27601 gcctatcggt caagaattga tgaaaatatg cggttccact ccagatattg cgtatttaaa
    27661 taaattcctc aacaaaataa atgttgaagg aagccaggta aaactatcaa ttattaatat
    27721 gtaaagaggt cgagttacct gtctgagttc cactaaccat aattaaaagc gaccgcaaaa
    27781 actgcccagt gaaaactgtg ttgccagtaa tcacaaatgc agcaagtact aataaagagc
    27841 ctgttaagcc cgcaaatatg tgggcttatt tttaattact cttcatcatc ccgcaggaac
    27901 tgacaaaaat cttcgctccc catcatatcg acagtgtttt taaattcgaa aaaagaaatt
    27961 cctgtcatag cacacacgcg ctggataatc agttttttct tttgctgtct gacggttgcg
    28021 gtttcaccaa actggataac agccaggact gtttcatcat atgatttcag gacaaaatca
    28081 caggttatat tctgcacttc acggattaaa tcttttacag cttcctcgct accatgagga
    28141 cgaacaaatt caatgagccg tattttggga aaaacggaat aatcaggtat aatattacgt
    28201 gaaagaatgc ctaaaaactc tgattcatta tttgagagta agggtacttt agtaaaacaa
    28261 tcaacctccc caatggctgt aaaagcattg gttgtcaaag ctggattgtt cttaataaaa
    28321 tcagagaata cattttcagc tgatggcaaa acaacagcag aattatgttg ttcatctcgt
    28381 tttttattac ggttaaatat catttcatat tccttttaaa agagcctgcg gcaggcacag
    28441 gcaaaaacat cgtttttgga caatctgatt caaatgttaa catttgacat cggattgtct
    28501 atcctgcatt gttctttact gtctgtaaga tcggcgctca ttcttccggc gttctcccga
    28561 taccaaaaag acggaacgca aaacggcttt cgatccacca ttcaaaaatc tgctctgcgc
    28621 aaccacaaac agttaaggcg atccatgcag caagaacaac gtaactactg acatctgcac
    28681 ccatgtacca ggcgatagtt aaacccatca ctgcgatacc gcctaaaaac tgcaacaaga
    28741 aacgaaatcc cgcaagaatc cccaatggaa taacaacaac cagataaagg gaagcagcca
    28801 gaaggtatct gacaggtgtc agcagataaa agaacctgga tattttcttt ttgtcagaat
    28861 aaacggcatc atttgcaaca tcggttgaat gatcattcat gctgtccctg attctttgaa
    28921 aatctacgat attatccttc atattgttat gacctcaatt ttcaggtggg tacattttat
    28981 caaacatatt agtaaactct gagtcttccc tggtatcgcc ctcataacca attgtataag
    29041 agaactttaa tttataaact tttctcccaa ttttttctga aactacgaaa cttaagttct
    29101 ttacttccgt atgtttcatg atttctttta ctgatttatt aagaacatct cttttaaaag
    29161 caggataatc tggatactta taatccttca ccccccccgc ccctattgta taaagattca
    29221 attcatcttt aagttcatct attgtcattt caaaactatt cgcgcgagaa ttattactat
    29281 atattttacg aatgagttga tatagtgatg cagcattaac acttgtcagt cgaacaacag
    29341 ataataaaac ttgcgttgta taacgttttt cttcaccaat aagtttacat aaaaatcttt
    29401 tcgcttcttt cgtaaacttg atccccactc taccttcatt cctgtaataa gtagaaaaaa
    29461 cagtcagact caaattcatt gaatcaggta cattactttt agtgaactta aatcccaaat
    29521 cactactcag ttctaatatt tcatcatgtt tcagcttaag aagcgtcgta tctaatatat
    29581 tagagccatc cctcaaagca agataagccg catcaggctt aacctgaacc catttaatgt
    29641 aatcggcaac tgtcacataa aatatgtgat catcatcgaa ttcattttta gagtttatct
    29701 ggcacatggc taaaaataag acttttttag cagccatagg caatgttgac agcgttgaat
    29761 tcaattcatt tcgatgtctc acttttgtta agcgagaaag ggaagtctta tttgtcgtca
    29821 ttctcattgt tccaacactc ttgataatca gactttaaca ccagaaacat gatattacaa
    29881 cacaatcaat atcatgttta accgagtatc ttctcatgtt tcatatctgt gatttatctc
    29941 acaaaggtaa tccatgctca aaatccacac tggattcaag gcagattaac aacagattca
    30001 aaatagattc accagaaaga acaacgaatt agtattctta tgacacattt tgtggatatc
    30061 accgaggaag ctctcatgtt taaccgagga agctctcatg tttaaccgag gaagctctca
    30121 tgtttaaccg aggaagctct catgtttaac cgaggaagtt atcatgttta accgaggaag
    30181 ctctcatgtt tagacaaaaa acacgcttaa aatcaataaa ttagagctac tctaagtttt
    30241 taagttttta agaaaaacaa gaaaagataa ggcttttaat gcgtgcgaat attcaggaac
    30301 tgtggataac tcaacaaaac cagcaacgat gatggttcct taccataaat ctgtcccctg
    30361 atgattatta cccgcaagct aaaacaaaca tcatcaatca ttaaaactca taactttcaa
    30421 taacataaaa tcatcaaaaa aatatttgat gccttatgta tctcaatttt caagaccata
    30481 aatttgtccc tagttaactt taacttattg aaataaatat aatattcgtg cgtactgatc
    30541 ctaatgtcac atcgcaaaaa ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
    30601 tcggctgcgg cgagcggtat ctcctttctc aaaaccagag agattcagat catatgatcc
    30661 gttatttgat cctttttgga tcttccacta agaagccctg aatcgcgctc tgccgcttcg
    30721 ttcgatacaa ccatgaggaa aaccatgcga aatgaaaaaa atcgcttaaa atgcgattca
    30781 gagcgtttta gggaggttct gttagttctc ctgcattgcg cccgtagttc gctaacgctc
    30841 acacgcgcag tacagcgccc cgccatgctg caatgcacca gggcggggta aactcgcttg
    30901 cgctcgcgct aactgcggca ttctgcggcg caaatcagca taatcatggc ctgctggtca
    30961 gttgggcttt gcagggtgaa gggatggcct gtgcaccctg gtacgttcgt cagcggctgc
    31021 gccgcgcttc ggggcgatgt ggctcctgtg ctgccctggt attttcgttc gccccttcca
    31081 tccgcttacg cggattaaaa aacgtgataa caatcactta tcgagtaaaa aaacgggaga
    31141 aaacttgcaa atacgactta aaggtcgtat tattgtttca tcgaaagggg aatggcccct
    31201 ctcacaatag cccagcaggg caggagatta aaaaaatgaa tatccaggaa gcattaaacg
    31261 tttttggatt atccggcgaa ttaactgaaa aagatatcaa agcagcatac agaaaagccg
    31321 ctttaaaata tcatccagat cgtaacccgt taggggctga actgatgaaa gcggtaaatg
    31381 cagcttttga tgtattgatg gcaaatattg ataaaataaa tcagttccag agcactgatg
    31441 aacatgcacg atataattac ggtgatgacc tggaaaaagt attaaacgtt ctttctggtt
    31501 tatctggtct ggtatttgaa gtgataggta actgggtctg gattagtgga gaaaccatta
    31561 cacataagga aactttaaaa gaaatcgggt gtaaatgggc ggcaaagaaa aaacaatggt
    31621 tttatcgtcc agacgaacat aaaagttact ggaatcgtga agaacacacg atagaagaaa
    31681 tccgcgcaaa atacggtaca accggacagc gcagggcgac agggtggcaa cgcgtggaaa
    31741 ccagagcgta accagaacgg gggcgagaag cccccgatat tatagccagc ccgtcttttg
    31801 gggcatcgaa cccaaaaaga cgggaacagc agccacgtag caaaaaggga aacagatgaa
    31861 ctcttttttt gaacaatatc accctgtctt tgaagttgtc tgccgtatcc tggggaacgg
    31921 ctggcgcgtg aacaaacttg atgattgttc gtctcgtata aaactgacgt caccgcagtt
    31981 taaaaattac tctgtgcata ttcggatgga aaaagaccgc ttttctgtgg tggggagtgt
    32041 ggatagtcgt tcctggcgta gtccgcatca tgtttgcacg ttatccagaa aacggaatcc
    32101 cgttgatatt gcagccgata tcgagcgaaa aattctggta aatgcttcgc aggaggtgtt
    32161 acaggcgatt gagtatgaga aacaccaggt ggaaaaaaag gacgaaattc tgatcctgaa
    32221 aggtatgtta tcgcaacttg tccagcttga aagctggtat ggggcattga caggctttaa
    32281 agctgaaaac ggattgaacg gtaaagtcac cgaacagggc gacagttatg atttacagat
    32341 tcggggcttg agtatagatc aacttgttaa aattacaggg tatttgaaac agttatgaga
    32401 aaaaataaag gtgatgtgac ttattttctt gaaaaagaag gtgacaatta caggcttaca
    32461 aaaaggataa aagccagaac aaatgttaaa atcgggaaca aaactacgaa aattacattg
    32521 tatgatgctg tactgaatga gaatgaatta caacatattg attttacgtg tgccggatta
    32581 agggaagacg atgagacgcc agttaagaat ttaattaagg agtttatgtt aaatgaaacc
    32641 cgataataca cctgaaaatg taaaaaaatt acgtttaaaa gcaggattaa cacaaaagga
    32701 atgcagtcat atttatggtg ttgggctaag aacctggcag aaaaaagagg aagttaatac
    32761 tcaaaacagt caaagtctgt cactggttga gtttgagttt cttttgttac ttgcaggaga
    32821 acatcctgaa tacgtgctct gcaaaaggga gtcaaaataa aatgacctgt gatggtttta
    32881 tattagtacc tggagttgcg ctcgtagctc gctgacgctc acacgcgcag tacagcgccc
    32941 cgccatgctg caatgcacca gggcggggta aactcgcttg cgctcgcgct aactgcggca
    33001 ttctgcggcg caaatcagca taatcatggc ctgctggtca gttgggcttt gcagggtgaa
    33061 gggatggcct gtgcaccctg gtacgttcgt cagcggctgc gccgcgcttc ggggcgatgt
    33121 ggctcctgtg ctgccctggt attttcgttc gccccttcca tccgcttacg cggattaaaa
    33181 aacgtgataa caatcactta tcgagtaaaa aaacgggaga aaacttgcaa atacgactta
    33241 aaggtcgtat tattgtttca tcgaaagggg aatggcccct ctcacaatag cccagcaggg
    33301 caggagatt
//
