LOCUS       NZ_CP063985             3173 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain 25DN plasmid p25DN-B, complete sequence.
ACCESSION   NZ_CP063985
VERSION     NZ_CP063985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16622485
            Assembly: GCF_015290725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Cao,G.
  TITLE     Complete genome of 25DN
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Cao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2020) Shandong Medicinal Biotechnology Center,
            Shandong First Medical University, 6699 Qingdao Road, Jinan 250117,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 10-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 420.0x
            Sequencing Technology             :: BGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 15:09:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,872
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 171 of 310
            Pseudo Genes (incomplete)         :: 133 of 310
            Pseudo Genes (internal stop)      :: 68 of 310
            Pseudo Genes (multiple problems)  :: 54 of 310
            Pseudo Genes (short protein)      :: 4 of 310
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="25DN"
                     /isolation_source="LAB"
                     /db_xref="taxon:562"
                     /plasmid="p25DN-B"
                     /geo_loc_name="China:Jinan"
                     /collection_date="2017-05"
     gene            complement(138..461)
                     /gene="mobA"
                     /locus_tag="IR210_RS24645"
                     /old_locus_tag="IR210_24650"
     CDS             complement(138..461)
                     /gene="mobA"
                     /locus_tag="IR210_RS24645"
                     /old_locus_tag="IR210_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            745..939
                     /locus_tag="IR210_RS24650"
                     /old_locus_tag="IR210_24655"
     CDS             745..939
                     /locus_tag="IR210_RS24650"
                     /old_locus_tag="IR210_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1427..1558
                     /locus_tag="IR210_RS24655"
                     /old_locus_tag="IR210_24660"
     CDS             1427..1558
                     /locus_tag="IR210_RS24655"
                     /old_locus_tag="IR210_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023151671.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
                     /name="hypothetical protein"
     gene            complement(1774..2202)
                     /locus_tag="IR210_RS24660"
                     /old_locus_tag="IR210_24665"
     CDS             complement(1774..2202)
                     /locus_tag="IR210_RS24660"
                     /old_locus_tag="IR210_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2166..2336)
                     /locus_tag="IR210_RS24665"
                     /old_locus_tag="IR210_24670"
     CDS             complement(2166..2336)
                     /locus_tag="IR210_RS24665"
                     /old_locus_tag="IR210_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            2494..2922
                     /locus_tag="IR210_RS24670"
                     /old_locus_tag="IR210_24675"
     CDS             2494..2922
                     /locus_tag="IR210_RS24670"
                     /old_locus_tag="IR210_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(471..707)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          975..1499
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.896"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(142..461)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 taccccggaa aaacaggaag gatgcgtcag gatcgttttc agctcgctgc atagctatgc
       61 atgaaagcga atggtgatca ctttgggagc ttacggtgtt cataccgtca gttttcgaca
      121 gttttctctc cgggaagcta atctgccata agcctggata acagggcacg gtgatactcc
      181 gtaatagcga tcagcacctc cgcatactcc ctgtccccga cacgcttgcc gtcgataaag
      241 agttttttct gtaacgcccc cagtctcata acttctttca gtacccgatc atcagtcagt
      301 gagttaactt tcttaccgag agctgccgcc cgtaaaaaac cggaaacggt cttgccgcat
      361 tcagcagccc tttttctgat ttcctggtct tcttctgccg tcagacgaac agggcgactg
      421 atagcccgcc tgcgcgtatt acttccactt cgttttgtca taaaacatgg ctccgtatct
      481 gacatgggtg tcggggcaaa gccctgacca gggcaattgt aatagcgtgc atgtatgcgc
      541 ggtataacaa ttgcacatcc tgtcctgtca gcaagctcga attaccgaca aaggcacggc
      601 agttttttgg cagtgacagt gttgtctgac agaaatgccc gtcaaaaagc caagcggcag
      661 aatagcggca gcaaaacagt cgaaaagtgg tacgcgatat aatcacacaa cgcagatgac
      721 agacagaacg gagaaaacga aaagatgaat aagcagcagc aagccgtact caacatggcg
      781 ggattcataa aaagtcagag cctgacgctg ctcgaaaaac tggacgcact caatgccgac
      841 gaacaggccg ccatgtgtga gaaactgcac gaactcgcag aagaactcca gaacagcata
      901 cagacacgct ttgaagcgga aaatcttacc gggatataac gcatccggac aggatgcaga
      961 acggcataga ggagcgtaag gagaaaatgt cacataaggc gctcttccgc ttcctcgctc
     1021 actgactcgc tacgctcggt cgttcgactg cggcgagcgg tgtcagctca ctcaaaggcg
     1081 gtaatacggt tatccacaga atcgggggat aaaaccggaa agaacatgtg agcaaaagac
     1141 aaagacccag aagaagggcg cgcgggaggc gtttttccat aggctccgcc cccctgacga
     1201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1261 ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1381 ttggtatctc ggttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1501 acacgactta acgccactgg caacggccac tggtaagcag gatgtgcgag agatgtaacg
     1561 agagttcttg aagtggcggc ctaactgagg ctacactgaa aggacagtat ttggtgacat
     1621 gtctcgcaca agactgttac cacggttaga gaatctgcca ggattaacta accttcgaaa
     1681 aaccacctgc cagggtggtt ttttcgtttt tacaatagtg agattacgac gagactgaat
     1741 gaatctcaag atttcattta gcctgccgct cgatcattca aagttttgag gtagcttcat
     1801 tgggtcatgg tatccaagct ctaaaagctt tatatggttc ggtgtcagcc gctggaaatg
     1861 aataacccag tcagaaacct tgtatttccc atgaaacgta tctttaaaaa ccggatcgcc
     1921 taaatgagca tgccataatt tgttagctct agcatacttc tcaatagctt ctttgttttc
     1981 gtaatgatca ggcactttcc acgatggcga tactttgcca gtccaagctg gatatccttt
     2041 taaaggatcg ccaaagagat tgtatttttc aaaatgctca atgaaatcat cgattttatc
     2101 gagtgttttt gcagaatatc tggatgtgta ataaaggttg aaggtctcag agaatgagac
     2161 aatattcaat tgtggatttt gcgcgcctgc tccgctgccc atgctcttct ctgctcccga
     2221 cttagaccag atggcattgt aacagacttc tcattcgctc tggaaatcaa cagagacaag
     2281 gatacgccaa aaacctcaat atctttaaca agatcatgac gttgttcggt tttcatatcg
     2341 ctaactccgt atctaattca ggcaggaaaa aatcttttcc tcgttttcag agtagtcttt
     2401 tttggttgca caataatggc gccagtgtca ccctattcat atgaatatac catataatat
     2461 atactttaaa tattttgggg cttaggaaaa tcaatgactt actccgttaa gaataaatgg
     2521 aaagctggcg gtttgtctct tggctggaca gcagtgccta ccgtattgtt ttttatacaa
     2581 aacgagaaaa agctaacttc agtggccttt aacacactac ttaacctgat cgttcactgg
     2641 tggagtttgc aagagtggcc gcatccgtca atggaaagtc tagccatacg tatgggggtt
     2701 tctgtacgga ctgttcagag agctatcaat gatcttgaaa aagctaatct cttggacaag
     2761 aaaccaacat cgaagagtga tcgccgatat ggtggtcgca atatttatga tctaacaaag
     2821 ctagttgact acctagatac aatgggtcca tcagtagctg aacaagttaa gaaaccacgc
     2881 cataaaaaac cggtatatac cgtaagaaaa acgacagctt aaaaaagcac aatggaactg
     2941 agcgcgagcg atgcacgata gcaaaacgat atattactta ttgcattggt cttggtagcc
     3001 ctgcttctgg ctaaaccact gtcaggagta attggctttg cctgtaatgg gttcatcgct
     3061 tcacagttaa agttatcgca tagctcatat atttaccata atcccttaat tgtacgcacc
     3121 actgaaacgc gctgaacgcg attctgacgc agacaccgca aaaagagatg ttt
//
