LOCUS       NZ_CP063837            11970 bp    DNA     circular CON 29-NOV-2023
DEFINITION  Klebsiella pneumoniae strain CRKP66R plasmid pCRKP66R-5, complete
            sequence.
ACCESSION   NZ_CP063837
VERSION     NZ_CP063837.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16604318
            Assembly: GCF_015277755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   He,F.
  TITLE     Plasmid-mediated tetA gene is an important factor that cause
            tigecycline resistance in carbapenem resistant Klebsiella pneumoniae
            ST11 clinical strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2020) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/29/2023 04:07:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,877
            CDSs (total)                      :: 5,755
            Genes (coding)                    :: 5,581
            CDSs (with protein)               :: 5,581
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 66 of 174
            Pseudo Genes (incomplete)         :: 116 of 174
            Pseudo Genes (internal stop)      :: 24 of 174
            Pseudo Genes (multiple problems)  :: 32 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063837.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP66R"
                     /isolation_source="induction culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRKP66R-5"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-06-13"
                     /collected_by="Fang He"
     gene            complement(join(11254..11970,1..15))
                     /gene="mobC"
                     /locus_tag="IRT47_RS29065"
                     /old_locus_tag="IRT47_29065"
     CDS             complement(join(11254..11970,1..15))
                     /gene="mobC"
                     /locus_tag="IRT47_RS29065"
                     /old_locus_tag="IRT47_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(15..1979)
                     /locus_tag="IRT47_RS29070"
                     /old_locus_tag="IRT47_29070"
     CDS             complement(15..1979)
                     /locus_tag="IRT47_RS29070"
                     /old_locus_tag="IRT47_29070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3463..3612)
                     /locus_tag="IRT47_RS29075"
                     /old_locus_tag="IRT47_29075"
     CDS             complement(3463..3612)
                     /locus_tag="IRT47_RS29075"
                     /old_locus_tag="IRT47_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3697..3954)
                     /locus_tag="IRT47_RS29080"
                     /old_locus_tag="IRT47_29080"
     CDS             complement(3697..3954)
                     /locus_tag="IRT47_RS29080"
                     /old_locus_tag="IRT47_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3964..5649)
                     /locus_tag="IRT47_RS29085"
                     /old_locus_tag="IRT47_29085"
     CDS             complement(3964..5649)
                     /locus_tag="IRT47_RS29085"
                     /old_locus_tag="IRT47_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5754..7241)
                     /gene="ltrA"
                     /locus_tag="IRT47_RS29090"
                     /old_locus_tag="IRT47_29090"
     CDS             complement(5754..7241)
                     /gene="ltrA"
                     /locus_tag="IRT47_RS29090"
                     /old_locus_tag="IRT47_29090"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7886..8131
                     /locus_tag="IRT47_RS29095"
                     /old_locus_tag="IRT47_29095"
     CDS             7886..8131
                     /locus_tag="IRT47_RS29095"
                     /old_locus_tag="IRT47_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8405..8776
                     /gene="tolA"
                     /locus_tag="IRT47_RS29100"
                     /old_locus_tag="IRT47_29100"
     CDS             8405..8776
                     /gene="tolA"
                     /locus_tag="IRT47_RS29100"
                     /old_locus_tag="IRT47_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8773..9138
                     /locus_tag="IRT47_RS29495"
     CDS             8773..9138
                     /locus_tag="IRT47_RS29495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9313..9591
                     /locus_tag="IRT47_RS29110"
                     /old_locus_tag="IRT47_29110"
     CDS             9313..9591
                     /locus_tag="IRT47_RS29110"
                     /old_locus_tag="IRT47_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9579..9893
                     /locus_tag="IRT47_RS29115"
                     /old_locus_tag="IRT47_29115"
     CDS             9579..9893
                     /locus_tag="IRT47_RS29115"
                     /old_locus_tag="IRT47_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10057..10485
                     /locus_tag="IRT47_RS29120"
                     /old_locus_tag="IRT47_29120"
     CDS             10057..10485
                     /locus_tag="IRT47_RS29120"
                     /old_locus_tag="IRT47_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10511..10690
                     /locus_tag="IRT47_RS29125"
                     /old_locus_tag="IRT47_29125"
     CDS             10511..10690
                     /locus_tag="IRT47_RS29125"
                     /old_locus_tag="IRT47_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10717..11247)
                     /locus_tag="IRT47_RS29130"
                     /old_locus_tag="IRT47_29130"
     CDS             complement(10717..11247)
                     /locus_tag="IRT47_RS29130"
                     /old_locus_tag="IRT47_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10649
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2782..3360
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11258..11970)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="98.0"
                     /evalue="1.21e-138"
ORIGIN
        1 ctgctccagt gtcatcagta aagccccgca aaatcatcat ccgcttttgg tccgccgccg
       61 tcggccggag gcaggtactc cgccagcggg tccgttttcg ccagcaccgg gaaggttttc
      121 agcgaaattt cccggcgctc aacccgcagc gggctgctga acgccagccg cgccacgccc
      181 agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc
      241 ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc
      301 cggctggccc ggcggcgctc cacgtccacc cggatctccc cggtctggcc cgccgcccac
      361 tgcgcggact cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc
      421 gtggttttca caaaatcagc gggcagatcc tgcccgcact gaccgaagtc gcccagcgac
      481 tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg
      541 tacttcgaca gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg
      601 aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg
      661 atcaccgcct catcgtccat cgagccgatg acgtacaggc agccgccgcc gttgattatc
      721 cccgccacat cgataccgct gtccgtctgc aggacgctca ggtcggccac gttctccagc
      781 ttggtgatca gccccttcac gtcctttttc agggcctccg gcaacgcgta cgccgccgcc
      841 agcacctgct gaatgtttgc cccctgcggg aactgctccg caatcagctt cgccgctttc
      901 tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cactcaggtt aaacccggca
      961 accagcagat tcttcagggc gtactggtcg agatcgcgga acagattcag ctgcgccggt
     1021 ttccccggcc gcagatccag cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc
     1081 gcccacgcgt cccctttggg gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag
     1141 cgaatgagcg cgttggtggc catcgcgctc ttgccgctcc cgggcaggcc gaggatctga
     1201 atattggtct tgcgccaggt atcacgaggg accagcaccg gctggccctg ctcatcctta
     1261 cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta
     1321 accggtaccg gctccccgaa atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg
     1381 ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa
     1441 tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc gacaagccct
     1501 ccggcacacc aggctatccc gaacaggtta gccggctgca gcaggtaata atctgcgttc
     1561 gcatagaggt ccgggcgcag gacggtttct ccccagctct cgataaacac ggcgacaacc
     1621 agccccccca gaagggccag accggcagcg ccggtcagcc agtacgcgta gttccagaca
     1681 tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg aggccagcac cgaaaatggc
     1741 acagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc cttatggctc
     1801 agccccacaa aacgcattcg gccggcccag agttcagcca gaccgaaaaa caggccgata
     1861 accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac ccacgggtaa
     1921 atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt attaaacatg
     1981 caatctctct ccttcagacg acgggtttac cggcgtgatg cgcagccccc gctgcacaag
     2041 ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct
     2101 ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac
     2161 aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt
     2221 gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc
     2281 agctttgctg cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg
     2341 agcctgacgg gggccgggtg aatttctggc gtggccggcg tccagccgga cagagccaga
     2401 aagaggggaa aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg tcttttcccc
     2461 tgaagtcagc cacgccccag cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa
     2521 cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc tatcctgata
     2581 attgatcgtc agggaataga aagacttaat caggggacaa tatcccacat cagcgcgtcc
     2641 ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct
     2701 ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg
     2761 gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc
     2821 ataggctccg ccctcctgcc agagttcaca taaacagacg ctttttcggt gcatctgtgg
     2881 gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga
     2941 tccccaccgt ttccggtagg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt
     3001 accggatacc tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca
     3061 cactggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc
     3121 cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggga
     3181 aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt
     3241 ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac
     3301 agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga
     3361 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     3481 cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt
     3541 tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt
     3601 tacctttttc atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg
     3661 aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc
     3721 aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa atggctgtag
     3781 taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat cctttaaagg
     3841 cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat actcttcccc
     3901 tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc ccatatttac
     3961 ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct gcttacccgt
     4021 tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt caagctcacc
     4081 gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc gggcccgctt
     4141 accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc caagtccctt
     4201 tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag tccccttgcg
     4261 aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt tctccttttg
     4321 tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg attcattcat
     4381 tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa tatccttatc
     4441 aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac
     4501 aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc
     4561 ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt
     4621 tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca gcccctcttc
     4681 cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac
     4741 ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc cggtctcttt
     4801 cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag ccggacgtga
     4861 atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa cacctttcgg
     4921 tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt
     4981 tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa ccgcaatatg
     5041 ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcctggtc
     5101 cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg acgtcataat
     5161 ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac cgtagatcgc
     5221 aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat cagcaacggc
     5281 ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg gagtagccag
     5341 ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag agctaccgct
     5401 atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc
     5461 cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct
     5521 gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt
     5581 cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc
     5641 accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc aacatgtcgc
     5701 ggtagggata cccgttaccg gatacccccc gcacagatcc cggcgtgcgc gatttacgca
     5761 ccgggctcct gcctcgggtg tctggcggtg aaccgctcca caggccatgg atgaagaacc
     5821 cgaacccttg gtagccatgc ggctgccagt ttgtttgctt tcgtccaggt cgtatcatcc
     5881 ttctggctcc tgcgcctgag cgcccggcgc cagaggtttg ttacgtgtgt cctgaacttc
     5941 tgcatggtgg ggaagttgcc cggtaccgag tgatagttca ggtatccctg aaccactctc
     6001 ctgagccatt ttccctgttc ggggattgag taatgccagc gccttcgcag accgtctttg
     6061 atggctttca gagttgccgt catccgatcc cggcgggtct ttcgtatcag catgaacctg
     6121 ccgttgcgat ctttcccgct gatgtgcgtg aacccgagga agttgaacgt ttctggtttg
     6181 ccttttcccc tgatggcacg gttttcggca gcgaagcggc cgaactccat cagacgggtt
     6241 ttctccgggt gaaccgtgag tccgaactcc ctcagtctgc gctgcatggc tatacggaag
     6301 cgccgggcat cgtatcgttt gtcgaacccg atgacgatgt catcggcgta tctgaccatt
     6361 accacattgc ctgtggcata gcgacgtcgc cactgatgcg cccacagatc gaagacgtag
     6421 tggaggtata tgtttgccag cagcggtgag atgaccgcac cctgtggggt gccttcctcc
     6481 gttgctcgcc attgaccctc ctccgacgtc ccggctgtga gccacttacg tatgagcctg
     6541 attaccctcc ggtcgccgat ccgatgctct gtgaacctga tcagccattc gtggctcacc
     6601 ctgtcgaaga actgactgat gtcggcatcc agtacccagt ttacgttagt gcgtaccagc
     6661 cctgtggcca gtgcgtccag tgcatcgtgc tggcttcgcc cgggtctgaa cccgtatgag
     6721 aaccccataa agtcgttttc atagactgcg ttcaggattt tcaccagcgc atactggacg
     6781 atcttgtcct ccagcgaggc gatgccgagc gggcgttgtt ttccatccgc ttttgggatg
     6841 tagtgacgcc tgccgggctg cgccctgtag ctgccctgat gtagcctccg gtgcagatct
     6901 gttatgttgt tcttcatgtt tccggcgtag tccatccacc tgatgccatc cactccggcg
     6961 gccgctttcc tgctcaggga gaggaatgcg gcttccagtg cttcgactgt cagcaggtgg
     7021 aacaatgctg taaaccgttc tttcttccgc tgcttcgcag cttcccgcac gcgtgacagc
     7081 ctctgtgaca tgctttcccg gctctgtgtc cggcgcatgt gtggctgttc cgcgttcccc
     7141 ttggccccgc tccttcgctc cactgactcc gctcctttcg ggttgttcgc ctgcttcgcc
     7201 gctactatga gcgagtccga cttctcctct ccgtacatca ccggctatga ctcctcgtct
     7261 tcccggtgcg ggccatctcc gacactggca gatggtcaga ggggagatct cccggttccc
     7321 gcgtagagat cgtattgaca tgccagggtc tcagaccccg ccgggtccat gtggcactcg
     7381 cagtatcgca ccctatgatg ttgccttccg ttaacagtac aacgtcggca cccggtaatt
     7441 taatatacat ttcgtggctc aatggctggc ctgtcaacac ccctgtcaac gcttcgcccc
     7501 atacctcgcg gtatgcaacg catgactcgg ggaccttgtg gattgctggt ccttcaatgg
     7561 tcggggactt tcaccccttg atctctaacc ggtctcccgg cgcacactgt acatatacac
     7621 agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga ttagttctca
     7681 tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct tttcccgaaa
     7741 atttctcatg gcaacaaatt ttattattta agacattcat cacacttaca tttcagatgg
     7801 tatagacaca tctgaaagct aactggcatg aggagaggac ctgcggttaa actgttcggg
     7861 aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa
     7921 gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag ggaacaaaac
     7981 gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga
     8041 acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac
     8101 cttcacctgg cagccacagc gccgccggta aggggtattt accataacgt cgcattgccc
     8161 gcaccgctca gacggtctga gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac
     8221 cacccggaaa accgccggaa acgcgcccgg taaattgtaa cccgctgcat gcctatgcac
     8281 gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat
     8341 gaacgtggcc gtaaagagtg acaaatatac atgtaacatt ttttactgac aggtcatcaa
     8401 cccgatgcgt ttaataccct ttatcctgct ggcattgtgc agccccttac tcctgacggc
     8461 ctgtaagcat tccaccaccc agccgccgaa aatttatact ctggataagt catctaaaat
     8521 cagtagctac gctgcggcca tcaagaccgc tgttgaaatg cagatgtttg aaccagaggc
     8581 ctatgcggga aaaatgtgca ccgtgcggat atccatacaa cgtgatggca ctctgaacag
     8641 cgccattgct gaaggaggag atccgaaact ctgtaaagca gcaatatcag ccgttaccca
     8701 cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa atattcaaaa acgcccctct
     8761 ggatttcaga ccatgaaagc ttttactctg acagtgctgg cggccatgtt cctcactccg
     8821 gtacatgcga taccactaaa acccgtaacc acactgcagc caacctaccc ggtacaggcc
     8881 gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg atattctgga cgatggacga
     8941 gtggacaacg tgtcgcttac cggctgtcac cttttttttc gggaaacgct gacggcaatg
     9001 aggcactggc tatatcccgc agggcacccg gcaatgcgac aggttataaa ttttgaattc
     9061 agtctgaaaa atgacggctt acaggatccg cgaccagtca ccacccaaaa gctcaatgat
     9121 gatggttgcc gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc tggatgatga
     9181 gtgagcctgt agcagccctg attagtggaa gcgaagctga aaaatgggca gcaaaagccg
     9241 tatttctggc aaatagcgcc gccacggatt aaagtagtca tgtgtaatac atatgactac
     9301 aaaggggctt ttatgtcatc agcaacatcc atcaggctcg atgaagaact taaggaccgg
     9361 ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg cgctaatgct ggaggcaatc
     9421 accgaataca tcgaccggga agagaagcgc agccagtacc tgcgggacgg acaggccgca
     9481 tggcagcact atcaggaaac gggtctgcac cttactgcgg aagaagcgga agcctggata
     9541 agtacctggg gaactgagaa cgagcaggat gctccgccat gccacaggta acgatttccg
     9601 cgctggcaca gcgcgattta caacgccttc aggactttct caaaaccaaa aatcggctgg
     9661 cagccagaaa aggaggtgag gtgatcgtcc gggctatcca gcaactgaaa acattgccag
     9721 acattggccg cccggtgccg tttctgccgc tggaatataa ggaactggtg atcgggtttg
     9781 gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga aatggaccag attgtgatcg
     9841 tcaccgtcag gcatcagaaa gaatccgggt atccgggggc agacagcctc taataaccga
     9901 tgacgggggc gcaattcatt gcgtggccgt cgccggtttg acaagcgcag cgcgtcagaa
     9961 aaccacatcc tgaacggaaa aacgggtaaa attcagcgat acatttaccg tgagaaatta
    10021 cacacctttc acacccgata aaatcagggg tatacagtgg cgaaaacatt gctcaacctt
    10081 tcacaggcag cacaagccgc gggcattacc agaagaacgc tatacaatca tgtaaaacag
    10141 ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg tcgtggatgt gtcagaactg
    10201 atccgtgtat acggaaatgt gaatatccct gagaaacaaa ttcccggcat ttcacaccgt
    10261 gaaaatacac aaaaaaattt cccacaggaa cagcttctgg caatgcaaaa ggaactggcc
    10321 gatctccggc aggctgtcac cctgatgctg gaagataaaa catcccggga agaagaacgc
    10381 cgccagcacg atgatgagcg ccggaaactc caggccgaag tggacagatt gacaacagaa
    10441 ctgacacaga agaaaaaacg cttctggtcc ggctggttta gctgagaact taccgaaggg
    10501 aatggagaac atgacgaagc aggaaaaaac tgccctcaat atggctaaat tcattaagga
    10561 tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac atggataccc cggccgacat
    10621 gtgcgaacgg cttcacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg
    10681 ggaagaataa cagcaccggc catgccggtg ctggcgttat tttttgccga acggccacca
    10741 ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc acgttaaaat agcggctgtt
    10801 atattcccgc atcagctccg ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc
    10861 gctgaccacc tcaatcatcg cgttaatctg ctccggtgag cacttcccgg catcaaccac
    10921 ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga tagaggtaat cacacagctt
    10981 cattcctgtg ggtttgtccc gttcccattg cggattttca ccggtgaaaa agtacgtggc
    11041 tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg
    11101 ctgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat
    11161 gtcttccaga tgctcgctgg ttggcataat gcagcgctcg ccgtctttgt cttccacgtc
    11221 ctcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca
    11281 cgcgaacagc ctctcccggt ccagctccac cgttttgcgg gcctcgccgg tctgtggatt
    11341 cacgatttca tccaccgcca gaatcgcccg ctgtacccgg acaagcgtcg gccggtcagg
    11401 gcagatgtac cagatttcat cgatgccgtt cgcccggcgg ttgaacagat acccggacac
    11461 cacttcctga taccgcctgc ggctcttgac ggtgcgctct acctcaaatg ccaccgttct
    11521 gccgtccagc tccgccagcg cgtccggcac tttcatccct ttcagggcca tacggtgcag
    11581 gtaccgccag ccggtggccc cccggccctc catggagagg cgcagggact gcacggccag
    11641 ccggtgcggc agctgcgccg ccgacaccct tgacggctga aacggaataa cgtccgtcag
    11701 cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc gtcagccccc acacgctgac
    11761 ctgacggccg tcaaccgggt atttcgcccg gaccagcagc cctttcgcca ccagactgtc
    11821 caggaagcgg taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa ccagctccgc
    11881 cgtggaacac agctcttccc gcagccagcg cagggtcagc cgtgttttct ccgcgttccg
    11941 cgcctgccgc ttcgcgtggc tgacgttgta
//
