LOCUS       NZ_CP063778             4073 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O25b:H4-ST131 plasmid pHK02-4, complete sequence.
ACCESSION   NZ_CP063778
VERSION     NZ_CP063778.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16520835
            Assembly: GCF_015277555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Jovanovic,O. and Shang,C.
  TITLE     Inactivation mechanism and efficacy of UV-LEDs at 265, 285 and 300
            nm for inactivation of ESBL-E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Jovanovic,O., Amabile-Cuevas,C.F. and Shang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Civil and Environmental Engineering, The
            HKUST, Clear Water Bay, Hong Kong 999077, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: FALCON v. Jun-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 14:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,045
            CDSs (total)                      :: 4,932
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 66 of 190
            Pseudo Genes (incomplete)         :: 142 of 190
            Pseudo Genes (internal stop)      :: 34 of 190
            Pseudo Genes (multiple problems)  :: 46 of 190
            Pseudo Genes (short protein)      :: 3 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063778.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /strain="ST131"
                     /serotype="O25:H4"
                     /isolation_source="wastewater"
                     /db_xref="taxon:941322"
                     /plasmid="pHK02-4"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2018-06"
                     /genotype="ESBL producer resistant to CAZ, CTX, AMC, CIP,
                     SXT, RL (EUCAST guidelines)"
     gene            join(2856..4073,1..942)
                     /gene="mobQ"
                     /locus_tag="INT78_RS24915"
                     /old_locus_tag="INT78_24920"
     CDS             join(2856..4073,1..942)
                     /gene="mobQ"
                     /locus_tag="INT78_RS24915"
                     /old_locus_tag="INT78_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1188..1889)
                     /locus_tag="INT78_RS24920"
                     /old_locus_tag="INT78_24925"
     CDS             complement(1188..1889)
                     /locus_tag="INT78_RS24920"
                     /old_locus_tag="INT78_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2423..2677)
                     /gene="mobC"
                     /locus_tag="INT78_RS24925"
                     /old_locus_tag="INT78_24930"
     CDS             complement(2423..2677)
                     /gene="mobC"
                     /locus_tag="INT78_RS24925"
                     /old_locus_tag="INT78_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2679..2855)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2856..3214
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1190..1889)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          20..939
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2770..4037
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.764"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cgccggaagg agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc
       61 aggaaaaatg acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat
      121 ggcgaggccc ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg
      181 aacggagaat acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag
      241 cgtgttgatt taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa
      301 catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt
      361 tgccagacat ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct
      421 ttctgtcttg gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat
      481 tttacgcagg gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa
      541 acccgccagc ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc
      601 cggcttaatg gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg
      661 gtcatcagtg cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat
      721 cacgatgcag acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc
      781 cgtgaggcac cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc
      841 cgctcgctgg gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca
      901 cacgccaccg agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt
      961 tttgtttaac atcgtgttat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     1021 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     1081 gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     1141 acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgattcta gatcgtcatc
     1201 ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg cccctttttg
     1261 atcgtctcaa ccgttacaac cagatcgctt ttttcgttca gttcggcgat ggctggcttg
     1321 attatcatct tattcaagtc tttgaattgt tgatatttat cttcaacggc aagcatggtt
     1381 cggaaatcat cgatagctat cgttctgtca ccggtgctgc ggaactgctg gagcagctcg
     1441 taaatgcgga tgctgtaggt gctggagagg ctggagacgt tcttcacgac tacccgcgta
     1501 aactgcccct tgagctgtgt cagatacggc atgatggcat cggagaaggt gatctctact
     1561 ttcgcctcac ctttgaaata ctgcgcccgg tactgcaccc agcggaactc ttctcgtttt
     1621 tcctcgttct tgatgattac tgacctgtcc cagaggcgat ctatcgcttc cttgagccta
     1681 gcttcagcgt gctgccgtcc catgtcagga aaggcatcat gaaactctgc agccgtgatg
     1741 gtcatggttt tttgttcatc cggcgaaggg gaatcttctc cggatttcag gcggctgata
     1801 cagaccagca ggaagcgctg ttcctgcaaa gtgagcttgt agcttgcctc gataagggcg
     1861 tttgatttgt aagctatcag atctgacatt tcgcttgctc gttgctattg gtggcgtttt
     1921 tttactgtac gccacccatc atattggtgg aagttttttt tttgcaaccc cttttttctg
     1981 tggattacac ccccaaaagc tccacctatc acccccaaaa gctccaccta tcacccccaa
     2041 aagctccacc tatcaccccc aaaagctcca cctatctcgc cagaacacct ttaaaatcat
     2101 gtggttacaa gacctaaaaa ggttttaaaa aaaaagatct aaaaataaga tcttaaaaac
     2161 ttaacggcga gcctgtggat aacttggggt gctgaaaata aaaatccaat aaaaacaatg
     2221 tgttgaaaaa aatcaggatt gaagattccg aatacacccc ctccaaccct gtggaaatcc
     2281 agtattcatg cgggttcagc gcatatcacc tcccgaaaaa ccgtgcgtat gtgctcaatc
     2341 aggcgacgga ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctggga
     2401 ctgcggcgag cggacgatac aatcactgtc ctggcagcgc ttccagctcg ttaatcagcg
     2461 gcagtatgcg cgtcacatca gcctgtcgtg tcacctctgc cttcaggaac tggagtaacc
     2521 aggcacgcaa ctgcacctcc tccggctttc tgacctttgc cagcaatgct gcacccacaa
     2581 tgaccttctg cgctgtgtcc agactcctgc tcttcgcctt caggcgctgt aatctggcct
     2641 cagcatcagc aatcttctgt tcgagtgttc tgctcatgat gtaactccgt gcgcgatgaa
     2701 aaatcgcatt ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggaaa
     2761 atcacgaagt gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg
     2821 ctgaaagaaa accacaaaaa ggcattacgg cagaaatggc gatttatcat ctcagcatga
     2881 aaatcatctc cagaaacagc ggctacagcg ccgttgcttc tgctgcctat cgttccggtt
     2941 cactgatgct tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg
     3001 ctgaagctgt gattctgact cccgcgacag cccctgcctg gtgtacgaac cgtgccgaac
     3061 tctggaatgc cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg
     3121 aactcgccat cccccgcgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg
     3181 tcagagaaaa ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata
     3241 aaacaaatcc ccatgcgcac atcatgctga ccacgagagc tgtcggacct gctggattcg
     3301 gtggaaaggt cagggactgg aacgaccgga cacatgccga gacgtggcgt gcatcatggg
     3361 ctgaccatgc gaacagggca ctggcgaacg ccggttatca ggaagagata gaccatcgtt
     3421 catacgagcg acaggggctt gagaaaacgc ctggtattca cctcggaaag tctgcctgtg
     3481 cgatggaaac acgcggtatc gaaacagaac gcggcgaaca gaatcgtctg attaacagac
     3541 ttaacctgga aatacagatt tcccgtaccg aactcagaaa cagcgggctg acagagcctg
     3601 cccgccgtgt ggctgacctt ctgaatatcg tgcttcctga caacgcaact tcgtacacac
     3661 tacgtgacat catcgaagcg ttgccgaagg acagtaatgc ggcatggaaa ctcaccagta
     3721 atgccatgtc gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagctgaaca
     3781 ccgaacgaaa ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg ccgttttcgt
     3841 ccggtttttc ccgtattccc ctgatgcaat gggcggcacc ggaataccgt agcgaacagg
     3901 gaaaaatcca gtcgctgagc cagcaaatgg aaaaattgcg acagcattac cggaaggtgg
     3961 agaaacagga cattcccgcg agtcagacgg catttgaaaa acagtggcag cagtgggggg
     4021 catccggcct tgcagctctc agagtccagt tgcttaaacg tgaagaggaa ttg
//
