LOCUS       NZ_CP063776             7937 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli O25b:H4-ST131 plasmid pHK02-2, complete sequence.
ACCESSION   NZ_CP063776
VERSION     NZ_CP063776.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16520835
            Assembly: GCF_015277555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Jovanovic,O. and Shang,C.
  TITLE     Inactivation mechanism and efficacy of UV-LEDs at 265, 285 and 300
            nm for inactivation of ESBL-E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Jovanovic,O., Amabile-Cuevas,C.F. and Shang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Civil and Environmental Engineering, The
            HKUST, Clear Water Bay, Hong Kong 999077, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: FALCON v. Jun-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/16/2023 14:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,045
            CDSs (total)                      :: 4,932
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 66 of 190
            Pseudo Genes (incomplete)         :: 142 of 190
            Pseudo Genes (internal stop)      :: 34 of 190
            Pseudo Genes (multiple problems)  :: 46 of 190
            Pseudo Genes (short protein)      :: 3 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063776.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /strain="ST131"
                     /serotype="O25:H4"
                     /isolation_source="wastewater"
                     /db_xref="taxon:941322"
                     /plasmid="pHK02-2"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2018-06"
                     /genotype="ESBL producer resistant to CAZ, CTX, AMC, CIP,
                     SXT, RL (EUCAST guidelines)"
     gene            complement(join(7776..7937,1..72))
                     /locus_tag="INT78_RS24840"
                     /old_locus_tag="INT78_24845"
     CDS             complement(join(7776..7937,1..72))
                     /locus_tag="INT78_RS24840"
                     /old_locus_tag="INT78_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(79..597)
                     /locus_tag="INT78_RS24845"
                     /old_locus_tag="INT78_24850"
     CDS             complement(79..597)
                     /locus_tag="INT78_RS24845"
                     /old_locus_tag="INT78_24850"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<635..1066)
                     /locus_tag="INT78_RS25345"
                     /pseudo=""
     CDS             complement(<635..1066)
                     /locus_tag="INT78_RS25345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1275..1598)
                     /locus_tag="INT78_RS24855"
                     /old_locus_tag="INT78_24860"
     CDS             complement(1275..1598)
                     /locus_tag="INT78_RS24855"
                     /old_locus_tag="INT78_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1663..1854
                     /locus_tag="INT78_RS24860"
                     /old_locus_tag="INT78_24865"
     CDS             1663..1854
                     /locus_tag="INT78_RS24860"
                     /old_locus_tag="INT78_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3460..3915
                     /locus_tag="INT78_RS24865"
                     /old_locus_tag="INT78_24870"
     CDS             3460..3915
                     /locus_tag="INT78_RS24865"
                     /old_locus_tag="INT78_24870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251120199.1"
                     /translation="MWHTFIQFTDAYTSFCEKYLGAYLHHYPNTNDMLKNEIRHVNEHG
                     ITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVRYSIKNINTIRIPKESISSDSYI
                     EKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKGCGAGCSCNSR"
                     /name="hypothetical protein"
     gene            3954..5546
                     /locus_tag="INT78_RS24870"
                     /old_locus_tag="INT78_24875"
     CDS             3954..5546
                     /locus_tag="INT78_RS24870"
                     /old_locus_tag="INT78_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            5549..6442
                     /locus_tag="INT78_RS24875"
                     /old_locus_tag="INT78_24880"
     CDS             5549..6442
                     /locus_tag="INT78_RS24875"
                     /old_locus_tag="INT78_24880"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(6467..6829)
                     /locus_tag="INT78_RS24880"
                     /old_locus_tag="INT78_24885"
     CDS             complement(6467..6829)
                     /locus_tag="INT78_RS24880"
                     /old_locus_tag="INT78_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(6826..7065)
                     /locus_tag="INT78_RS24885"
                     /old_locus_tag="INT78_24890"
     CDS             complement(6826..7065)
                     /locus_tag="INT78_RS24885"
                     /old_locus_tag="INT78_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     Typing          complement(1931..2010)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          2043..2585
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(36..1285)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.367"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(1282..1631)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
ORIGIN
        1 gttcagccgc tgcatatagt cctgttgcag ttgctctaag gcgttcagca aatgtctttc
       61 cagctccgtc actctgtgtc actccgtcag ttgtacccag tccttcccct gatagggaat
      121 caccgttgcc gatttcccct gagggataac cagaaatttc cttctcccgt cctgcacaaa
      181 ctccacgccc catgtcttcg cgttcagttt ctgcaatgtt tcttcctgta tcctgatttc
      241 ttccaggttc gcctgtatca tcccgccaag ataccagagc gtcccgccac tcacggtaaa
      301 caggaaaagg accatcccca gtaacatcat gcccgtattc cctgccagct ttaacacgtc
      361 cttcctgtgc tgcatcatcg cctctttcac cccttcccgg tgttttttca gtgattcctc
      421 tgtcgaagct gtgaacaggg ctatagcgtc tttgattttc gtctcgtttg atgtcacagc
      481 cttgcttaca gatttttcga gcttgctgaa ctcgtcgttc agcattgtct ctgtagattc
      541 ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa
      601 atgctccttt cagcctgata ttcttcccac cgttcgggtc tgcaatgctg atactgcttc
      661 gcatcaccct gaccacttca agccctgcct ctgtgagcgc ctgaatcaca tcctgacgac
      721 cttttatctc cccgacatga taaagagcgt ctatcccgcg tgtgacgctc tctgcaagcg
      781 cctgtttcgt ttccggcagg ttatcaggga gagtcagtat ccggcggttc tccggggcgt
      841 tggggtcatg cagcccgtaa tggtgattca ccagcgtctg ccaggcatca attctcggcc
      901 tgtctgcccg gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atgttcggaa
      961 tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact
     1021 gattcttttc gagaccgggc atcagtacac gctcaaagct cgccatcacc ttttcacgtc
     1081 ctcccggcgg cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa
     1141 atggcgtggc atcgatgagt tcccggactt cttccggatt accctgaagc accgttgccc
     1201 cttcccggtt tcgctccctt cccagcaggt aatcaaccgg accactgcca ccgccttttc
     1261 ccctggcatg aaatttaaca atcatcccgc gcgccctgtt ccctgacggc cagacgcagc
     1321 cgacgcagct cctccccgat ggccatcagc gcagccaaca cctgaacccg atcaccggaa
     1381 gaccactgcc cgctgttcac cttcctcgct gtctgattca ggttattccc gatggcggcc
     1441 agctgacgca gtaacggcgg tgccagtgtc ggcagcttcc cggagcgtgc aaccggctca
     1501 cccaggcaga cccgccgcat ccataccgcc agctgtttac cctcacagcg ttccagtaac
     1561 cgggcatgtt catcatcagt aacccgtatg gtgagcatcc tctcgcgttt catcggtatc
     1621 attaccccat aaaacagaaa tcccccttac acggaggcat cagtgactaa acaggaaaaa
     1681 accgccctta acatggcccg ttttatcaga agccagacat taacgctgct ggagaaactc
     1741 aacgaactgg acgcagatga acaggccgat atttgtgaat cacttcacga ccacgccgac
     1801 gagctttacc gcagctgcct cgcacgcttc ggggatgacg gtgaaaacct ctgacacata
     1861 cagctcccgg agacggtaac agcttgtctg tgagcggatg ccgggagcag acaagcccct
     1921 cagggcgcgt cagggggttt cggggcgaag ccctgaacca gtcacgtagc gatagcggag
     1981 tgtatactgg cttaactatg cggcatcagt acgaattgta tggaaagcgc accatgtccg
     2041 atgtgaaatg ccgcacagat gcgtaaggag aaaatgcacg ttccggcgct cttccgcttc
     2101 ctcgctcact gactcgctac actcggtcgt tcggctgcgg cgagcggtgt ctgctcactc
     2161 aaaagcggag atgcggttat ccacagaatc aggggataac gccaaaagaa acatgtgagc
     2221 aaaaaacgag aaccaggaaa aggctgcgcc gttggcgttt ttccataggc tccgcccccc
     2281 tgacgagcat cataaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2461 acgctgtagg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2581 ggtaagacac gacaaagcgc cactggcagc agccactggt aactggacaa ggtggattga
     2641 gatattaaga gttcttgaag tggtggccta actgcggcta cactagaagg acagtatttg
     2701 gtatctgtgc tccaccaagc cagttacccg gttaagcagt ccccaactga cttaaccttc
     2761 gactaaaccg cctccccagg cggttttttc gtttactggc agcagattac gcgcagaaaa
     2821 aaaggatctc aagaagatcc tttgatcttt ttgagatccg cccactcctg gaacctacaa
     2881 cacatgtgta aaacgtcaat ggagggggct attatcggac tgcaacacat ttcgtacagc
     2941 ctttacactc ggtgaattag cggccctaga tgcatccact cattcaacca actcaattct
     3001 cttctcttag agtgggaaaa ttttgttttc cttgcagccc ctacccgtaa aaactggctg
     3061 caaaaacctt gtattactaa tggatcctat aaccattaaa aaacttgatt actatctcat
     3121 ttatagaatt aggcatattg acacaagcac aaatttaaca ataccataac aaatgataac
     3181 tattctcatc tacattcaaa tatataattg ggggtgttat gctacaacat aagatgaaca
     3241 gcagttctta tgcaaaagtt cataatgtta gctcattaga agatatcatg agttatcaca
     3301 atgatgatgt tcttctaaaa tttcgtaaag aatggaatgt aaccagaaga agctgatgat
     3361 atctttaatg aaacaaagaa attcatctgg ctagcctcaa catgcctaac ggaatgctac
     3421 aatataaaag ttcacgagca attacaaatt atagatgaaa tgtggcatac gtttattcaa
     3481 tttacagatg cttataccag cttttgtgaa aaatatcttg gtgcttacct ccaccattat
     3541 ccaaatacaa atgacatgct aaaaaatgag ataaggcatg ttaatgagca tggtataaca
     3601 ttccaagaat atcgttttaa cgaatataaa aatcaaattg aaaaaatcgc tttttaccta
     3661 ggtcatgaaa ctgtcgcaaa gtggtatggt gattatgctg taagatacag cataaagaac
     3721 attaatacta taagaattcc aaaagaatcc atatccagtg attcttacat cgaaaaagta
     3781 aagagtatca ctcaccttcc agccgcagaa tttgtaaaaa taataatgcg aaaagatgtt
     3841 tggaatgata atggttctgt ttgtggttgc agcggtaaag gatgtggcgc tgggtgctca
     3901 tgtaattcta gataacataa agcccgtaat atacgggctt taaggattat aaattgaatt
     3961 ttattgaaaa gtacatcatc acatttaata aatgtaattt gttttttatt atattattat
     4021 caactataag tagttttctt ttcgttctat tcggatattt aataaaaaca gttattgaca
     4081 ataaagattc aataggagaa ataaactcat tattaatatt tatagcatgt ttcttatcaa
     4141 ttagattcct tatgcctgct ggatatagca tatcggaata cttgactcaa aaaacaaata
     4201 tagaattatc tgtaaagctt agagagcaag ttattgataa tatacaaaat tctcaccaag
     4261 agcatttttt aaagaaaaat aaaggagaac taaataaggt tatagaaagc atgctttctt
     4321 ctgcatcatc attattttat acaatatgtt ctgatgtagt accattatta atacaaatga
     4381 ttggaataat cataacaatt tgtataagtg ttaatacatt aatagccatt gagtttataa
     4441 taataatggc aatctatata atatttgtca taaaaatgac acaacgtaga tttccaatga
     4501 tgaaatcagt tgcacttagc tccaaattcg catctggaag aatgtttgat atgatgcaca
     4561 tgtatcctat ggataaagct ttccatacca cggataaaag tcgtaaacga gtaattcaag
     4621 ctgtaaatac acattcagaa aaacaaagaa aagtaaataa tgaatttttc ctgtttggta
     4681 tcagctccgc ttttctttca gtcattttta gtagtctgat tatcctatca gcatactgga
     4741 tgtttcttca tggtagagca agccgtggta gtattattat gcttgccact tttttattcc
     4801 aagtttttct cccattaaat cgcattggtt atctatttag acaaataaaa atggcaagaa
     4861 cagaaattga tttatactgt gccgaaataa acgacataaa aaaaacaaat tacacagaca
     4921 agcaatatct tcatgtaaaa gacaatattt gcaatattga aatatacaac aaatcattca
     4981 accataaatt aatattaaca aaaggcgttg ttacctttat aaccggtgag aatggttcgg
     5041 gaaaaacaac tattgcaaaa attctctctg gcaatataac aacagaagaa aacacaataa
     5101 aaatcaatgg gatacaacaa gaaaaagcaa attctccttt tgttaatgtc ttatacgttc
     5161 ctcaagatct agatctcatg cctggaacta tagaggaaaa tatattacat tattccggca
     5221 ttaataattt aaatacaatt aaaaagctgc tacacagatt taagttcgac aaaccactag
     5281 attatgagat caaaggttac ggacataatt tatctggtgg acaaaaacaa aaattaggag
     5341 tatctcttac atctggaaaa aacgtagact tgattatttt cgatgaaccg acaaaagggt
     5401 ttgattcact tggtataaaa ataatcagtg attatataaa agaggaaaac aaggaaaagc
     5461 atatcattgt tatatctcat gatgaacaac ttataaataa tatacctaaa gcacagatta
     5521 tagatatcac aaaacatgag gtttaaaaat gaataatctt ataaaaaagg aaatcataga
     5581 aaaatttaag aaatataatt tccagaaata tccgtttgta ttcacggatg ttaattataa
     5641 aaacttaatc aactggaatg atcttaataa attgcttgaa aaagatatat tgcactatcc
     5701 tagagttaga atggcaaatg acaactttcc cgaaattagg gggtataaag gatttataag
     5761 atacacatat agccaaacag gcgatagaac accacatata aatcgccatc agctatataa
     5821 atgcttacgt gatggcgcaa cccttatagt agatcgatgc cagtcattct ttgaatctgt
     5881 agatgaaagc agactatggt tatctaaaga gttagaatgt acatgtagtg ctaatctata
     5941 tgctgcattt acagcaacac caagctttgg gcttcatttt gacaatcatg atgtaatagc
     6001 tgttcaaatt gagggaataa aaaaatggaa agtttacaac cctacctatt cataccctct
     6061 cgaagatgaa agaagcttcg attatctacc acctaatacc tccccagatt atgagtttga
     6121 tataacccca ggtcaggcca tatatcttcc tgctggatac tggcacaatg ttacgactca
     6181 gagcaaacac tctcttcata tatcttttac agttataaga cctcgtcgat tagaactatt
     6241 taaaacatta tttgatgaat taaaaaacaa tccatatatg cgggaaccta tagagcatgg
     6301 tgattcatta tctgataaag aaaaaataaa aactataata actaatgcta taaatagttt
     6361 tgatattcag tttgcagaaa acatgcttaa agctcaaagc agaacttatc gctataaaaa
     6421 aataaaactt gaacatatat aaccattaat ctaagggggc ttccccttag attttttcat
     6481 acgatataaa ctgcattttc catccaccat taacctttat ccaattctca ataaaagcca
     6541 ttaatatatt tccatcattt tgctttattt cagctttccc tgaaacacaa gcaatgcctt
     6601 taaactcacg cattacgaca tcatattcat atctttctat gctaggtaaa atcctcgctg
     6661 gacgcatatc aatctttcgt aactgatgtt ttttatagat aactttttga ccatttgttg
     6721 aaataaacca atcagcttcc aagtaatcta gcatttcagt attacctgaa taaaatgcat
     6781 tataccattc ttttcttatt agatttaagt ctgatttaaa attagtcaca ctaccttcct
     6841 catatctaat tcctcctcta atctatcccc aacattcctt actgatttta tagcatcatc
     6901 aatagccaca ttatatatga ctgggattac tcttttaaga atctcatcaa gtaattcctc
     6961 tgattcaaac tgtccaaggc atatatcatg ttcatctctg attttttgtg aaataaattc
     7021 agctagtcct ttatatatgt ttttttcaat atcagaaatt ttcattcttt atgaagcctc
     7081 acaaataaat taataatata acatttttcc tatcatagga aaattacaac tcaaccatac
     7141 tgcaacctgg aatttcccaa gcaagcatat ttaaataagt gcaggatacc acctcaaatc
     7201 attctctaag gaacaagata atttaccata atcccttatt gtacgcaccg ctgaaacgcg
     7261 ttcggcgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg aaaaactgcc
     7321 gcgatcgcac ccggtaaatt ttaaccacat gcatagctat gcagccatgt gaatcacgca
     7381 ggaattgcca gccagagaca gctgaaacgg atttttttca tgctttcgga agcgaagaag
     7441 acggggaccg gagcgggaaa acagaagttc aggggaatga gcgcgatctg gcaatagagg
     7501 caaaacacag caacaaaaga cacaccagaa tcgcgcccgt atgcgtttta acgcgttcct
     7561 gtgttttcag caaggtctga ctgaagcgcg ttcccggtgc ccccgaacca accattatcc
     7621 caaaaaatgc accggaaacg cgctgacaac attttagcct gtctctgatt accatcccag
     7681 cgaagggcca tcctgtgtcc gttcctgtat ttccagctgg cgctcacgct ccagggataa
     7741 ctcatgttcg cgttcctgta tcagccgttc atcccttatc tcctgttctc gctgtataac
     7801 tggctcaagc gttctgtctg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc
     7861 cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acattttctg caattccacg
     7921 aaggcgctct cccattc
//
